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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Estudo Comparativo das t?cnicas de Imunofluoresc?ncia Direta, Imunofluoresc?ncia Indireta e Colora??o de Sellers em amostra de tecido nervoso de c?es e de animais silvestres para o diagn?stico da cinomose. / Comparative study of the techniques of Direct Immunofluorescence, Indirect Immunofluorescence and Stain Sellers in samples of nervous tissue of dogs and wild animals for diagnostic of the canine distemper

Barreto, Michelle Gomes 27 July 2006 (has links)
Made available in DSpace on 2016-04-28T20:17:24Z (GMT). No. of bitstreams: 1 2006-Michelle Gomes Barreto.pdf: 634856 bytes, checksum: 61550da2cbc0a0c4fbc8b6781fe93da0 (MD5) Previous issue date: 2006-07-27 / It was performed a study comparative in the diagnostic of the canine distemper through techniques of Direct Immunofluorescence (IFD), Indirect Immunofluorescence (IFI) and Stain Sellers in samples of nervous tissue of dogs and wild animals that died with suspicion clinical of the viruse. Were utilized 118 samples of central nervous system (SNC) of domestic dogs and 3 wild animals, 2 skunks and 1 monkey, utilizing Ammon?s horn (CA), cerebellum (CB) and brain stem (TE). The TE was the region of larger percentage of positive, following of CA. The comparation between Stain Sellers and IFD, this last demonstrated a large sensitive. The IFI observed what to the realization of the technique was necessary the utilization of fresh materials. The utilization of the 117 samples in conditions of autolysis presented negative result. However 1 sample not autolysate, it was result positive in the 3 brain regions (CA, CB, TE). It was not possible the comparative with the technique of IFI, because in the work the technique alone was possible in materials conserved, with the advantage of a read more easy. In the work was detected also canine distemper in a skunk through technique of IFD. / Foi realizado um estudo comparativo do diagn?stico da cinomose pelas t?cnicas de Imunofluoresc?ncia Direta (IFD), Imunofluoresc?ncia Indireta (IFI) e Colora??o de Sellers em amostras de tecido nervoso de c?es e de animais silvestres que morreram com suspeita cl?nica da virose. Foram utilizados 118 amostras de Sistema nervoso central (SNC) de c?es dom?sticos e de 3 animais silvestres , dois gamb?s e um mico, utilizando-se Corno de Ammon (CA), Cerebelo (CB) e Tronco Encef?lico (TE). O TE foi ? regi?o de maior porcentagem de positivos, seguido do CA. A compara??o entre a Colora??o de Sellers, e a IFD, revelou uma maior sensibilidade nesta ?ltima. Na IFI, observamos que para a realiza??o da t?cnica torna-se necess?rio ? utiliza??o de material fresco. Tendo em vista a utiliza??o das 117 amostras em estado de aut?lise apresentaram resultados negativos e apenas 1 n?o autolisado, teve seu resultado positivo nas 3 regi?es cerebrais (CA, CB e TE). N?o foi poss?vel a compara??o com a t?cnica de IFI, porque nesse trabalho, a t?cnica s? foi poss?vel em materiais conservados, com a vantagem de uma leitura mais f?cil. Nesse trabalho foi detectado tamb?m cinomose em um gamb? pela t?cnica de IFD.

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