No description available.

This paper examines two English polyverbal constructions, COME to V2 and GET to V2, as exemplified in Examples 1 and 2, respectively. (1) the senator came to know thousands of his constituents (2) Little Johnny got to eat ice cream after every little league game. Previous studies considered these types of constructions (though come and get as used here have not been sufficiently studied) as belonging to a special class of complement constructions, in which the infinitive is regarded as instantiating a separate, subordinate predication from that of the “matrix” or leftward finite verb. These constructions, however, exhibit systematic deviation from the various criteria proposed in previous research. This study uses the American National Corpus to investigate the statistical propensities of the target phenomena via lexico-syntactic (collostructional analysis) and morpho-syntactic (binary logistic regression) features, as captured through the lens of construction grammar.

Proteins exist in two conformers. The trans conformation is favored by the most of the amino acids. The proline residue due to its unique geometry has a high probability of being in the cis conformation. Thus the cis/trans isomerisation of the peptide bond preceding the proline residue becomes a rate limiting step in the folding and unfolding of the proteins. The enzymes which catalyze this rate limiting step were discovered by Fischer in porcine kidney and called as peptidyl prolyl cis trans isomerases (PPIases). There are four families of the PPIases. They are the parvulins, cyclophilins, FKBPs and trigger factors. All the four families catalyze a common reaction and the give rise to a stable trans product. We therefore wanted to analyse if cross complementation exists across the PPIase families. Our analysis has shown that the prokaryotic and the PPIase domain of the eukaryotic parvulins show a high structural similarity. The catalytic residues were found to be conserved across the genera. Our study has shown that a single domain 92 amino acid long prokaryotic parvulin PpiC from E.coli could complement for the function of Ess1 in Saacharomyces cerevisiae. We have also shown that under conditions of over expression the carboxy terminus of NifM from Azotobacter vinelandii could functionally replace Ess1 in S. cerevisiae. However the complete nifM was unable to do so. We have shown that the amino terminus of NifM acts as a regulatory unit not only for the PPIase activity of its carboxy terminus domain but also for the PPIase activity of PpiC and human Pin1. Using random mutagenesis we have identified the potential docking sites on amino terminus of NifM. These sites are defined as the residues which are responsible for the regulatory activity of NifM. Further we have found that FKBPs which show a high similarity with human Pin1 was unable to isomerise substrate specific to the parvulins. Our analysis has shown, the substrate binding pocket in FKBP is large due to its aromatic nature. Hence it is unable FKBPs to complement for the function of the parvulins.

The focus of this thesis is the description and analysis of clausal complementation in Sundanese, an Austronesian language spoken in Indonesia. The thesis examined a range of clausal complement types in Sundanese, which consists of (i) yén(/wi)/réhna 'that' complements, (ii) pikeun 'for' complements, (iii) sangkan/supaya/ngarah/sina 'so that' complements, (iv) raising complements, (v) crossed control complements, and (vi) nominalizations. This varied set of complement structures display distinct properties in terms of the sort of elements admitted in the complements. The theoretical aspect of the thesis is the examination of two important generalizations: (i) that complementation is a universal feature of human languages (Noonan 1985, 2007); and (ii) the well-accepted precept that finiteness plays a role in the world's languages. This thesis provides evidence that Sundanese evinces (syntactic) complementation and that any claim to the contrary is unfounded. In terms of finiteness, despite the lack of overt morphological manifestations of finiteness, the thesis argues that finiteness seems to be at work in Sundanese and that it operates as it does in other languages to account for the distribution of overt subjects. In addition, the body of data presented herein is also germane to a host of other theoretical issues, especially with regard to Austronesian languages. The first is inclusion of VoiceP in a clausal structure. Following (Sukarno 2003, Son 2006, Son & Cole 2008, Cole et al. 2008, Ko 2009 and Legate 2011), the thesis adopts an additional functional layer above vP, i.e. VoiceP, to harbor voice marking. It is proposed that in Sundanese transitives, both actor DPs in active sentences and actor PPs in passive counterparts are arguments and are therefore merged in the same slot, i.e. Spec,vP. The second theoretical point investigated in this thesis is whether Raising to Object and Proleptic NP constructions are alike or different. In this thesis, I claim that the two types of constructions should be analyzed as instantiations of two distinct structures, mainly due to structural properties: Raising to Object involves movement, while prolepsis does not. The next theoretical issue has to do with a subset of control predicates, which exhibits behaviors atypical of canonical control. I propose a slightly different analysis that draws upon earlier accounts (Polinsky& Potsdam 2008; Fukuda 2008; Nomoto 2008, 2011, Sato & Kitada 2012). On the basis of (a) the presence of a plural marked-verb inside the crossed control complement and (b) the apparent parallelism between the ordinary control and the crossed control, I postulate that the structure for the two types of control of the same predicates is identical, in which case their complement includes VoiceP. The last theoretical concern is related to the fact certain nominal structures display verb-like properties. Following Alexiadou (2001), the present thesis proposes that, like verbal structures, some nominals contain functional projections such as AspP, VoiceP and VP. This naturally explains why nominals exhibit verbal properties that they do.

Thesis (Ph.D.)--University of Delaware, 2007. / Principal faculty advisor: John T. Koh, Dept. of Chemistry & Biochemistry. Includes bibliographical references.

No description available.

Thesis (Ph. D.)--Cornell University, May, 2009. / Vita. Includes bibliographical references.

Commonly, when it is desirable to replace an essential gene with an allelic series of mutated genes, or genes with altered expression patterns, the complementing constructs are introduced into heterozygous plants, followed by the selection of homozygous null segregants. To overcome this laborious and time-consuming step, the newly developed two-component system utilizes a site-specific recombinase to excise a wild-type copy of the gene of interest from transformed tissues. In the first component (the first vector), a wild-type version of the gene is placed between target sequences recognized by FLP recombinase from the yeast 2 μm plasmid. This construct is transformed into a plant heterozygous for a null mutation at the endogenous locus, and progeny plants carrying the excisable complementing gene and segregating homozygous knockout at the endogenous locus are selected. The second component (the second vector) carries the experimental gene along with the FLP gene. When this construct is introduced, FLP recombinase excises the complementing gene, leaving the experimental gene as the only functional copy. The FLP gene is driven by an egg apparatus specific enhancer (EASE) to ensure excision of the complementing cDNA in the egg cell and zygote following floral-dip transformation. The utility of this system is being tested using various experimental derivatives of the essential sucrose-proton symporter, AtSUC2, which is required for photoassimilate transport.

Linguistics / This dissertation examines the complexity of complementation in O’dam, also known as Southeastern Tepehuan (SET), based on a corpus of twenty-seven hours of naturally recorded speech (105 texts). This complexity is due in part to the fact that the same subordinate marker, na, encodes complements, adverbial and relative clauses, and, in some instances, non-embedded clauses. That is, distributional patterns indicate that na is a polyfunctional marker in SET. In addition to using the na marker, SET conveys adverbial and complement clauses through using non-embedded clauses (i.e., juxtaposition), supporting the notion that subordination does not always involve an embedded association (Cristofaro 2003). Crucially, juxtaposition is used as a coordination strategy. Therefore, investigating clause linkage in SET highlights the formal and semantic categories in which SET differentiates embedded clauses. It further suggests that SET has a continuum of features that distinguish these dependent relationships (e.g., aspect, second position clitics, inherent control, an overt subordinate marker, negation, and focus); thus, this research contributes to recent work on the typology of complementation. All embedded clauses in SET can be distinguished by means of a second position clitic and by the morphology attached to the embedded predicate or to the subordinate marker. More specifically, complements and relative clauses require second position clitics, but adverbials only use them if they are marking switch-reference. This behavior is unique, because adverbials use second position clitics as an indicator of thematic continuity for subjects, suggesting that the development of these clitics evolved independently with the function of marking switch reference. Also, ‘when’ clauses do not have a fixed order compared to locative and manner adverbial clauses, because locative and manner adverbial clauses, along with complements and relatives, always follow the main clause. As for the morphology encoded in complement clauses, SET distinguishes between embedded clauses with or without a complementizer, and on the basis of internal aspectual morphology and inherent control. As a result, it is not the form, but the interface of morphosyntactic, semantic and pragmatic information that helps us identify the type of embedded clause we are facing. / text

Eukaryotic protein kinases are one of the most important classes of human proteins, and a great deal of research has focused on the development of small molecule inhibitors as biological probes for the determination of their cellular function or as therapeutics for the treatment of disease, such as cancer. The need for new selective inhibitors and a better understanding of the selectivities of existing small molecules is readily apparent. Towards the goal of better understanding protein kinases and the molecules that inhibit them, I have developed a split-protein-based approach for the investigation of these kinase-small molecule interactions. Employing split-firefly luciferase as a reporter domain, we engineered a three-hybrid system capable of determining kinase inhibition through competitive interactions between an active site-directed ligand and a small molecule of interest. This method measures luciferase activity as a function of ligand binding, as opposed to the more traditional assays which quantify kinase activity directly, and alleviates the laborious process of protein purification. The model kinase PKA and the promiscuous ligand staurosporine were used in an initial test case to successfully validate the general design principles of our assay. The modular nature inherent to the assay's design enabled us to adapt it to roughly 300 additional protein kinases and two different ligands. We were able to establish a protocol for rapidly ascertaining the inhibition of a kinase by a library of 80 commercially available kinase inhibitors in a 96-well, high-throughput format. This protocol was then systematically applied to the AGC group of kinases to observe patterns of inhibition across similarly related kinases. We have further shown how these results might be correlated with the sequence identity between kinases to better anticipate inhibitor promiscuity. Finally, we were able to illustrate how a kinase-centric approach could be applied to correlate alterations to the kinase domain with changes in luminescence. This has use for the interrogation of different modes of inhibition as well as in identifying the specific determinants of inhibitor binding. In total, these efforts represent the optimization of a new, general platform for determining kinase inhibitor selectivity across the kinome, and it could potentially be applied universally to the interrogation of protein-ligand interactions.