Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-01-10T12:12:36Z No. of bitstreams: 1 LucianaAraujoNascimento_TESE.pdf: 3313657 bytes, checksum: 8d85adb333c8ffbf5a14771e4ac06475 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-01-11T11:48:21Z (GMT) No. of bitstreams: 1 LucianaAraujoNascimento_TESE.pdf: 3313657 bytes, checksum: 8d85adb333c8ffbf5a14771e4ac06475 (MD5) / Made available in DSpace on 2017-01-11T11:48:21Z (GMT). No. of bitstreams: 1 LucianaAraujoNascimento_TESE.pdf: 3313657 bytes, checksum: 8d85adb333c8ffbf5a14771e4ac06475 (MD5) Previous issue date: 2016-02-24 / O objetivo do trabalho ? observar o efeito da purifica??o da quitosana (Qt) sobre a forma??o de filmes automontados utilizando complexos inorg?nicos: Na2[Fe(CN)5NO] (NPS) e cis-[Ru(bpy)2ImN(NO)](PF6)3 (RubpyNO), caracteriz?-los pelas t?cnicas de Uv-V?s, Infravermelho e voltametria c?clica. A Quitosana comercial foi purificada em tr?s diferentes formas: neutra, cloridrato e acetato. A caracteriza??o dessas apresentaram diferen?as significativas com rela??o as suas caracter?sticas como umidade, grau de desacetila??o, viscosidade e massa molar. Foi realizado o monitoramento pela t?cnica de Uv-vis do crescimento dos filmes de QtNeutra/NPS; QtAcetato/NPS; QtCloridrato/NPS; QtNeutra/ RubpyNO; QtAcetato/ RubpyNO e QtCloridrato/ RubpyNO. Dentre eles, a quitosana purificada na forma acetato mostrou melhor intera??o com os complexos, formando filmes com melhores ?ndices de correla??o R e deposi??o homog?nea dos materiais a cada bicamada formada. Essa intera??o foi confirmada pela an?lise do espectro do infravermelho dos filmes que apresentou bandas de ambos os compostos. Os estudos eletroqu?micos mostraram que os filmes formados possuem grande estabilidade, melhor resolu??o quando analisados a uma taxa de varredura de 50 mV.s-1 e que as amostras QtNeutra e QtAcetato com NPS apresentam os potenciais de pico melhores definidos sofrendo processos quase revers?veis de degrada??o. Os testes de rea??o de ?cido asc?rbico com os filmes de QtAcetato/NPS e QtAcetato/ RubpyNO mostraram que o primeiro apresentou uma melhor resposta eletroqu?mica. / Chitosan (Qt) has great technological application due to present atoxic features, biodegradable and renewable. The fact that a cationic polymer makes it capable of forming films by selfassembly technique, allowing its interaction with various compounds. In this context, this study aimed to evaluate the influence of purification of Qt in the neutral, acetate and hydrochloride forms in building self-assembled films by their interaction with nitrosyl complex (SNP and RubpyNO). Analysis of the degree of deacetylation, viscometry, infrared and thermal analysis showed that the purified Qt have different characteristics. The formation of the films was monitored by spectroscopy in the Uv-Vis region and characterized through spectroscopic techniques infrared (FTIR), thermal analysis (TG/DTG), cyclic voltammetry (CV), scanning electron microscopy (SEM) and energy dispersive X-ray (EDS). The results showed that Qt neutral and acetate forming films linear and homogeneous growth, with the interaction of both materials confirmed by FTIR technique. The results show that cyclic voltammetry of the neutral and acetate Qt films and the complex behave as semi-reversible processes, except the film QtAcetato/RubpyNO, which presents itself as an irreversible process. It was observed that chitosan affects the purification method presented in the redox process for the films due to the shape of weaker or stronger interaction of the particles according to the load presented by polycations and polyanions in solution during the formation of automounted film. The images obtained by SEM and EDS confirmed the presence of layers arranged with uniform coverage and homogeneous distribution of the elements in the film surface. The TG/DTG analysis confirmed thermal stability of the films before and after being subjected to VC. Reaction rolls of film with ascorbic acid (AA) and cysteine showed that the film QtNeutra/RubpyNO showed the best response to both substances. A straight line equation has been suggested for the determination of AA by QtNeutra/RubpyNO film and for the determination of cysteine, additional studies are required.