Darwinian sexual selection predicts that males with the most extravagant secondary sexual traits suffer elevated mortality. Although correlative evidence has generally not borne this idea out, recent research, including a field cricket study, showed that investment in sexually selected traits is costly to survival. I investigated male survival, ornamentation (song) and mating success in a North American grylline, Gryllus pennsylvanicus, to test the generality of previous work and highlight the importance of ecology differences to resource allocation. As the calling songs of older male G. pennsylvanicus are highly attractive to females, in Chapter 2 I tested whether male age correlated with calling song and found a weak but statistically significant correlation, thus leaving open the possibility that choosy females use an age-based indicator mechanism. In Chapter 3, I tested the condition dependence of male survival and calling effort. In contrast to previous work, I found that high condition males both called more and lived longer than low condition males, although there was no trade-off between survival and calling effort. The substantial condition dependence of calling effort suggests that calling effort is under strong directional selection. In Chapter 4 I tested whether female mating preferences resulted in strong selection on male calling effort. I also tested for the condition dependence of female mating preferences. I found that female choosiness was condition-dependent, but the rank of preferred male songs (preference function) was not. Both low and high condition females preferred high calling effort over low calling effort song. In Chapter 5 I tested for evidence of nonlinear selection on male survival that might explain the nonlinear pattern of male investment in survival seen in Chapter 3 (i.e. male survival leveled-off with increasing condition). I found that socially experienced females, but not virgin and naive females, exerted linear selection on male age. I discuss these and the other results of my thesis in the context of previous work on field crickets and condition-dependent ornamentation. Finally, Appendix A reports results that confirm ancient Chinese cultural knowledge that large headed male crickets are more successful in male-male combat.

Darwinian sexual selection predicts that males with the most extravagant secondary sexual traits suffer elevated mortality. Although correlative evidence has generally not borne this idea out, recent research, including a field cricket study, showed that investment in sexually selected traits is costly to survival. I investigated male survival, ornamentation (song) and mating success in a North American grylline, Gryllus pennsylvanicus, to test the generality of previous work and highlight the importance of ecology differences to resource allocation. As the calling songs of older male G. pennsylvanicus are highly attractive to females, in Chapter 2 I tested whether male age correlated with calling song and found a weak but statistically significant correlation, thus leaving open the possibility that choosy females use an age-based indicator mechanism. In Chapter 3, I tested the condition dependence of male survival and calling effort. In contrast to previous work, I found that high condition males both called more and lived longer than low condition males, although there was no trade-off between survival and calling effort. The substantial condition dependence of calling effort suggests that calling effort is under strong directional selection. In Chapter 4 I tested whether female mating preferences resulted in strong selection on male calling effort. I also tested for the condition dependence of female mating preferences. I found that female choosiness was condition-dependent, but the rank of preferred male songs (preference function) was not. Both low and high condition females preferred high calling effort over low calling effort song. In Chapter 5 I tested for evidence of nonlinear selection on male survival that might explain the nonlinear pattern of male investment in survival seen in Chapter 3 (i.e. male survival leveled-off with increasing condition). I found that socially experienced females, but not virgin and naive females, exerted linear selection on male age. I discuss these and the other results of my thesis in the context of previous work on field crickets and condition-dependent ornamentation. Finally, Appendix A reports results that confirm ancient Chinese cultural knowledge that large headed male crickets are more successful in male-male combat.

The ultimate goal of my thesis is to develop a better understanding of the contribution of indirect benefits (i.e. good genes) to the evolution of female mate preferences. It is genetic variance in, and genetic correlations (covariances) among, male sexual displays, female preferences for them, and fitness that in part determine the degree to which females preferring certain male displays over others will gain an indirect benefit by having higher fitness offspring. Recent advances in quantitative genetic theory provide the mathematical means for quantifying the strength of indirect selection for female mate preferences (Kirkpatrick and Hall 2004), at least under certain conditions, but there are few empirical systems for which such data exist (Brooks and Endler 2001; Qvarnström et al. 2006). I have undertaken a classic half-sibling breeding design with the ultimate goal of estimating the specific parameters of this model in a population of the Australian fruit fly Drosophila serrata. The breeding design was performed across two environments - one to which the population was well adapted and a novel environment to which it was not - thereby also providing insight into genotype-by-environment interactions for this suite of traits and their effects on good genes indirect benefits in a novel environment. General insight is also gained into the genetic covariance of male and female fitness and the prevalence of intralocus sexual conflict, the quantitative genetic basis of female mate preferences for multiple male traits, the condition-dependence of these traits, and the genetic association between sexual displays and fitness when mutation-selection balance is inferred. My results advocate caution in the application of existing theory to quantify the strength of indirect selection, suggesting that a good genes process may be fundamentally different when the exaggeration of sexual displays is eventually halted and an equilibrium is reached between opposing selection.

The ultimate goal of my thesis is to develop a better understanding of the contribution of indirect benefits (i.e. good genes) to the evolution of female mate preferences. It is genetic variance in, and genetic correlations (covariances) among, male sexual displays, female preferences for them, and fitness that in part determine the degree to which females preferring certain male displays over others will gain an indirect benefit by having higher fitness offspring. Recent advances in quantitative genetic theory provide the mathematical means for quantifying the strength of indirect selection for female mate preferences (Kirkpatrick and Hall 2004), at least under certain conditions, but there are few empirical systems for which such data exist (Brooks and Endler 2001; Qvarnström et al. 2006). I have undertaken a classic half-sibling breeding design with the ultimate goal of estimating the specific parameters of this model in a population of the Australian fruit fly Drosophila serrata. The breeding design was performed across two environments - one to which the population was well adapted and a novel environment to which it was not - thereby also providing insight into genotype-by-environment interactions for this suite of traits and their effects on good genes indirect benefits in a novel environment. General insight is also gained into the genetic covariance of male and female fitness and the prevalence of intralocus sexual conflict, the quantitative genetic basis of female mate preferences for multiple male traits, the condition-dependence of these traits, and the genetic association between sexual displays and fitness when mutation-selection balance is inferred. My results advocate caution in the application of existing theory to quantify the strength of indirect selection, suggesting that a good genes process may be fundamentally different when the exaggeration of sexual displays is eventually halted and an equilibrium is reached between opposing selection.

The ultimate goal of my thesis is to develop a better understanding of the contribution of indirect benefits (i.e. good genes) to the evolution of female mate preferences. It is genetic variance in, and genetic correlations (covariances) among, male sexual displays, female preferences for them, and fitness that in part determine the degree to which females preferring certain male displays over others will gain an indirect benefit by having higher fitness offspring. Recent advances in quantitative genetic theory provide the mathematical means for quantifying the strength of indirect selection for female mate preferences (Kirkpatrick and Hall 2004), at least under certain conditions, but there are few empirical systems for which such data exist (Brooks and Endler 2001; Qvarnström et al. 2006). I have undertaken a classic half-sibling breeding design with the ultimate goal of estimating the specific parameters of this model in a population of the Australian fruit fly Drosophila serrata. The breeding design was performed across two environments - one to which the population was well adapted and a novel environment to which it was not - thereby also providing insight into genotype-by-environment interactions for this suite of traits and their effects on good genes indirect benefits in a novel environment. General insight is also gained into the genetic covariance of male and female fitness and the prevalence of intralocus sexual conflict, the quantitative genetic basis of female mate preferences for multiple male traits, the condition-dependence of these traits, and the genetic association between sexual displays and fitness when mutation-selection balance is inferred. My results advocate caution in the application of existing theory to quantify the strength of indirect selection, suggesting that a good genes process may be fundamentally different when the exaggeration of sexual displays is eventually halted and an equilibrium is reached between opposing selection.

In many species carotenoid-based ornaments plays fundamental role in sexual selection. It is believed that carotenoid-based ornaments act as indicators of individual quality and condition during mate-choice, as associations between their expression, individual fintess and various condition indicators were observed. Despite the long-term research, mechanisms ensuring signalling honesty remain unknown. Currently, the most accepted hypothesis suggests antioxidant properties of carotenoids, and, therefore, a trade-off between their allocation to ornamentation or defence against free radicals. The antioxidant function of carotenoids in vivo was challenged in birds, however, and alternative hypotheses were proposed, assuming either carotenoid loss due to free-radical oxidation, or harmful (pro- oxidant) effects of high carotenoid levels in the body, or interconnection of metabolic pathways for both, the carotenoid conversion and homeostatic control of mitochondrial respiration and redox state. These hypotheses assume differing allocation of carotenoids under elevated oxidative stress, which we tested using experimental manipulations of oxidative state and carotenoid intake in adult male zebra finches (Taeniopygia guttata). The results are inconsistent with all the alternative hypotheses, but partly...

Environmental factors can have profound effects on the strength and direction of selection and recent studies suggest that such environment-dependent selection can be sex-specific. Sexual selection theory predicts that male fitness is more condition dependent compared to female fitness, suggesting that male fitness is more sensitive to environmental stress. However, our knowledge about the effect of environmental factors on sex-specific reproductive performance and on sex differences in the opportunity for selection is still very limited. In the present study, we investigated the sex-specific effects of diet quality (yeast deprivation and flour type) in the red flour beetle Tribolium castaneum. Specifically, we manipulated yeast supplementation in wheat and whole-wheat flour in competition assays allowing us to test for sex-specific effects of food quality (i) on reproductive success and (ii) on the opportunity for selection. Our data show that yeast deprivation in wheat flour had significantly negative effects on body mass and reproductive success of both sexes, while high-quality flour (whole-wheat flour) was able to buffer the negative impact to a large extent. Importantly, our data suggest no sex-specific effect of dietary stress on reproductive success because the magnitude of the negative effect of yeast deprivation was similar for males and females. Moreover, our study demonstrates that low food quality inflated the opportunity for selection and did not differ between sexes neither under benign nor stressful dietary conditions. We discuss the implications of our findings for the adaptation to stressful environments.

Sexual ornaments important for mating success in many species are often assumed to evolve as condition-dependent signals of individual quality. Ornament expression can be associated with age and survival, thereby signalling individual viability. Here, we have tested viability signalling function of tail streamers and their importance for within-pair and extra-pair fertilisation success in the European barn swallow (Hirundo rustica rustica). In contrast to previous studies on this subspecies, our data suggest that tail length is not associated with fertilisation success in our population. Instead, the most important predictors of within-pair and extra-pair fertilisation success were female and male age, respectively. Our data supported viability signalling function of male tail streamers, as documented by age-related within- individual increase in their length. There was no evidence for senescence in this trait. Contrary to some previous studies, the viability signalling function of tail streamers was further supported by observed selective disappearance of males with shorter tails. Several physiological mechanisms have been proposed as maintaining signalling honesty. Among them, oxidative stress from highly reactive species (RS), including free radicals, attracted a considerable attention. Given...