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31	Vliv povlakování na lomové chování ledeburitické oceli / The influence of coatings on fracture behaviour of ledeburitic steel Šafář, Martin January 2010 (has links) This work deals with tool steel Vanadis 6. It describes the preparation of samples for the three-point bending test, with which it examines the influence of surface roughness, the effect of nitriding, coating and duplex coatings on fracture behaviour of the steel. First, it examines bending strength and the total energy required to work of fracture. The work also includes mapping and measurement of surface roughness on different layers. This measurement is performed on a confocal microscope.
32	Zařízení pro měření koncentrace intracelulárního vápníku v LabVIEW / System for measurement of concentration of intracellular calcium in LabVIEW Pochlopeň, Timotej January 2014 (has links) Master‘s thesis deals with the methods and system for measurement of concentration of intracellular calcium in LabVIEW. The thesis covers the theory of fluorescence principles, fluorescence methods and indicators for measurement of concentration of intracellular calcium. The procedure of measurement by means of fluorescence microscope, camera and indicators is also mentioned. The theory dealing with confocal microscopy is presented and two techniques are compared. Literary solution is presented. The practical part describes the program with a graphical user interface used for acquisition and analysis of data measuring dynamics of intracellular calcium concentration. The experimental part describes the preparation and measurement of cells and evaluation of experiments.
33	Preclinical exploration of novel small molecules as anticancer agents in triple-negative and HER2/neu-positive breast cancers Weng, Shu-Chuan January 2008 (has links) No description available. Biomedical Research Cellular Biology Pharmacology breast cancer HER2/neu triple-negative breast cancer tamoxifen estrogen receptor HDAC Akt PDK-1 hsp90 fluorescent polarization autophagy LAMP-2 LAMP-1 apoptosis celecoxib SAHA MS-275 Confocal microscope
34	Micro-Spectroscopy of Bio-Assemblies at the Single Cell Level Kera, Jeslin 01 January 2017 (has links) (PDF) In this thesis, we investigate biological molecules on a micron scale in the ultraviolet spectral region through the non-destructive confocal absorption microscopy. The setup involves a combination of confocal microscope with a UV light excitation beam to measure the optical absorption spectra with spatial resolution of 1.4 μm in the lateral and 3.6 μm in the axial direction. Confocal absorption microscopy has the benefits of requiring no labels and only low light intensity for excitation while providing a strong signal from the contrast generated by the attenuation of propagating light due to absorption. This enables spatially resolved measurements of single live cells and bio-molecules with less than 10^9 molecules in the probe volume. Employing a multichannel detection system, the absorption spectrum of hemoglobin in a single red blood cell is measured on the timescale of seconds. We also extend the spectral range from the visible range to the experimentally more challenging ultra-violet region where characteristic absorption bands of bio-molecules are observed. Exploiting the ultra-violet range, amino acids, nucleic acids solutions, and plant cells are investigated. We measure the spatially resolved absorption spectra at the nucleus of an onion cell and cytoplasm to probe DNA base-pair absorption. Small variations in our micro-absorption data are seen around 260 nm, possibly due to the abundance of DNA in the nucleus. This thesis contributes to the goal of spectroscopic identification of spatial heterogeneities at the single cell level and the label-free detection of proteins and nucleic acids. Spectroscopy Confocal Microscope Absorption and Transmission Amino Acids Nucleic Acids Nitrogen bases Micro experiments Analytical Chemistry Biochemistry Bioinformatics Biological and Chemical Physics Biophysics Botany Cancer Biology Cell Biology Investigative Techniques Molecular Biology Molecular Genetics Optics Organic Chemistry Plant Biology Polymer Chemistry Structural Biology

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