Global ETD Search

1	Populationsgenetische Aspekte von Inkompatibilitätssystemen bei Basidiomyceten / Krafzig, Dirk. January 1995 (has links) (PDF) Techn. Universiẗat, Diss.--Braunschweig, 1995.
2	Elucidation of the product synthesis of the sesquiterpene synthase Cop6 isolated from <em>Coprinus cinereus</em> Andersson, Marie January 2009 (has links) <p>Mushrooms are believed to have a great potential for production of bioactive metabolites e. g. terpenes, a group of interesting compounds with diverse chemical properties such as antitumour and antibacterial activity. Cop6 is a terpene cyclase isolated from the mushroom <em>Coprinus cinereus</em> that catalyzes the cyclization of farnesyl diphosphate (FPP) to mainly α-cuprenene. In this study gas chromatography combined with mass spectroscopy (GC-MS) is used to analyze the product profile of Cop6 mutants created by PCR based site directed mutagenesis. The goal is to produce trichodiene, the parent hydrocarbon in the biosynthesis of trichothecene antibiotics and mycotoxins. Valine instead of tyrosine in amino acid position 195 resulted in cyclisation of (E)-β-Farnesene and (3Z,6E)-α-Farnesene besides the products of the wild type enzyme. Another mutant with aspartic acid instead of asparagine in position 224 resulted in the synthesis of β-Bisabolene except for α-cuprenene and methionine in position 74 instead of isoleucine killed the activity of the cyclase. Furthermore, an attempt to saturation of position 98 was made, resulting in four mutants. Two of them essentially killed the activity of the cyclase whereas two had minor effect of the product profile compared to the wild type. </p> Site directed mutagenesis PCR Gas Chromatography-Mass Spectrometry sesquiterpenes Coprinus cinereus Molecular biology Molekylärbiologi
3	Elucidation of the product synthesis of the sesquiterpene synthase Cop6 isolated from Coprinus cinereus Andersson, Marie January 2009 (has links) Mushrooms are believed to have a great potential for production of bioactive metabolites e. g. terpenes, a group of interesting compounds with diverse chemical properties such as antitumour and antibacterial activity. Cop6 is a terpene cyclase isolated from the mushroom Coprinus cinereus that catalyzes the cyclization of farnesyl diphosphate (FPP) to mainly α-cuprenene. In this study gas chromatography combined with mass spectroscopy (GC-MS) is used to analyze the product profile of Cop6 mutants created by PCR based site directed mutagenesis. The goal is to produce trichodiene, the parent hydrocarbon in the biosynthesis of trichothecene antibiotics and mycotoxins. Valine instead of tyrosine in amino acid position 195 resulted in cyclisation of (E)-β-Farnesene and (3Z,6E)-α-Farnesene besides the products of the wild type enzyme. Another mutant with aspartic acid instead of asparagine in position 224 resulted in the synthesis of β-Bisabolene except for α-cuprenene and methionine in position 74 instead of isoleucine killed the activity of the cyclase. Furthermore, an attempt to saturation of position 98 was made, resulting in four mutants. Two of them essentially killed the activity of the cyclase whereas two had minor effect of the product profile compared to the wild type. Site directed mutagenesis PCR Gas Chromatography-Mass Spectrometry sesquiterpenes Coprinus cinereus Molecular biology Molekylärbiologi
4	Light effects on fruiting body development of wildtype in comparison to light-insensitive mutant strains of the basidiomycete Coprinopsis cinerea, grazing of mites (Tyrophagus putrescentiae) on the strains and production of volatile organic compounds during fruiting body development Chaisaena, Wassana 17 July 2008 (has links) No description available. 000 Allgemeines Wissenschaft Forest Sciences and Forest Ecology Coprinopsis cinerea Coprinus cinereus AmutBmut Tintlingsartige Fruchtkörperentwicklung Tyrophagus putrescentiae Milben dst gen VOCs Coprinopsis cinerea Coprinus cinereus AmutBmut Inkcap mushrooms Fruiting body development Tyrophagus putrescentiae Mites dst gene VOCs 48.00 Land- und Forstwirtschaft
5	Growth, fruiting body development and laccase production of selected coprini / Navarro González, Mónica Navarro González, Mónica 30 April 2008 (has links) No description available. 570 Biowissenschaften, Biologie WA 000 Biologie Allgemeines Mathematics and Natural Science Coprinus Coprinopsis Coprinellus Parasola Tintlingsartige Coprinopsis cinerea Coprinus cinereus Coprini Fruchtkörperentwicklung Coprinoid Pilze Anamorph Phenoloxidase Laccase Kupfer Laccasegenexpression Milben Tyrophagus putrescentiae Coprinus Coprinopsis Coprinellus Parasola Inkcap mushrooms Coprinopsis cinerea Coprinus cinereus Coprini Fruiting body development Coprinoid mushrooms Anamorph etiolated stipes Phenoloxidase Laccase Copper Laccase gene expression Mites Tyrophagus putrescentiae 42.00 Biologie: Allgemeines 42.13 Molekularbiologie 42.51 Mycophyta Lichenes

1

Page generated in 0.0605 seconds