Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2018-03-27T18:48:03Z No. of bitstreams: 1 2017 - Tays Araujo Camilo.pdf: 3555895 bytes, checksum: 8b6818596f8654186472296bc3b528aa (MD5) / Made available in DSpace on 2018-03-27T18:48:03Z (GMT). No. of bitstreams: 1 2017 - Tays Araujo Camilo.pdf: 3555895 bytes, checksum: 8b6818596f8654186472296bc3b528aa (MD5) Previous issue date: 2017-03-07 / CNPq / Infectious Bronchitis Virus (vBIG) is a highly contagious viral agent among the species Gallus gallus, being considered one of the pathologies that most affects small and lare world poultry producers. Vaccination as a form of prevention to this disease, has not been effective, due to the great genetic variability found. As in the world, in Brazil there are already several studies characterizing the genetic variability found in this virus. In Rio de Janeiro, poultry farming suffers from the disease, but there is no recent study to analyze the genetic variability of the strains found, as a way of supporting epidemiological studies that may help in the future, in the choice of Protocols. The objective of this study was to characterize the S1 gene of the Chicken Infectious Bronchitis virus (vBIG) in chickens farms in the southern region of Rio de Janeiro, as well as to evaluate the genetic variability of the S1 gene in a BIG outbreak on a farm Of laying hens of the northern region of. Tissue samples were collected from animals presenting BIG-compatible clinical signs, such as weight loss, respiratory problems and low productivity. Samples of collected tissues were stored in solution of RNA Later and formalin 10% buffered for molecular and histopathological diagnoses, respectively. In order to perform the molecular analyzes, RNA extraction techniques, as well as RT-PCR (Reverse Transcriptase, Polymerase Chain Reaction), real-time PCR (RT-qPCR), Nested PCR (RT-nPCR), and Sequencing of some samples collected. For the histopathological analyzes, cleavage and preparation of microscopy slides were performed. The results obtained from the sequencing were compared to the sequence of the Ma5 vaccine strain as reference strain, in addition to other sequences deposited on GenBank, exhibiting a variable identity percentage of 72.41% to 100%. Among the samples from each farm, there was variability, even within the same bird. All sequences analyzed were described as genotype 1, belonging to strains 1 and 11; In the comparison of amino acids, there were changes in the hypervariable regions of the virus in the sequences classified as BR-1, being able to explain the low cross-protection that have been occurring in Brazilian plants. These results demonstrated the importance of virus identification, since it becomes an important tool in epidemiological surveillance and in the elaboration of efficient vaccine protocols. / O v?rus da Bronquite Infecciosa das Galinhas (vBIG) ? um agente viral altamente contagioso entre a esp?cie Gallus gallus, sendo considerado uma das patologias que mais acomete a avicultura mundial. A vacina??o como forma de preven??o a esta doen?a, n?o tem sido eficaz, devido a grande variabilidade gen?tica encontrada. Assim como no mundo, no Brasil tamb?m j? existem diversos estudos caracterizando a variabilidade gen?tica encontrada neste v?rus. No Rio de Janeiro, a cria??o av?cola sofre com a doen?a, por?m ainda n?o existe um estudo recente com o objetivo de analisar a variabilidade gen?tica das cepas encontradas, como uma forma de apoio a estudos epidemiol?gicos, que possam auxiliar no futuro, na escolha de protocolos vacinais mais adequados. Este estudo teve como objetivo realizar a caracteriza??o molecular do gene S1 do v?rus da Bronquite Infecciosa das Galinhas (vBIG) em granjas de frangos de corte na regi?o Sul fluminense, como tamb?m avaliar a variabilidade gen?tica do gene S1 em um surto de BIG em uma granja de poedeiras da regi?o Norte fluminense. Foram realizadas coletas de tecidos dos animais que apresentavam sinais cl?nicos compat?veis com BIG, como perda de peso, problemas respirat?rios e baixa produtividade. Amostras de tecidos coletados foram armazenadas em solu??o de RNA Later e formalina 10% tamponada para os diagn?sticos molecular e histopatol?gico, respectivamente. Para realiza??o das an?lises moleculares foram utilizadas t?cnicas de extra??o de RNA, bem como RT-PCR (?Reverse Transcriptase, Polymerase Chain Reaction?), PCR em tempo real (RT-qPCR), ?Nested? PCR (RT-nPCR), e sequenciamento de algumas amostras coletadas. Para as an?lises histopatol?gicas foram realizadas clivagem e elabora??o de l?minas de microscopia. Os resultados obtidos do sequenciamento foram comparados a sequ?ncia da cepa vacinal Ma5, como cepa refer?ncia, al?m de outras sequencias depositadas no GenBank, apresentando um percentual de identidade vari?vel de 72,41% a 100%. Dentre as amostras de cada granja, houve variabilidade, at? mesmo dentro de uma mesma ave. Todas as sequencias analisadas foram descritas como gen?tipo 1, pertencentes as linhagens 1 e 11; Na compara??o de amino?cidos, houve mudan?as nas regi?es hipervari?veis do v?rus nas sequ?ncias classificadas como BR-1, podendo explicar a baixa prote??o-cruzada que v?m ocorrendo nos plant?is brasileiro. Estes resultados demonstraram a import?ncia da identifica??o g?nica do v?rus, pois se torna uma ferramenta importante na vigil?ncia epidemiol?gica e em elabora??es de protocolos vacinais que sejam eficientes.