Seventeen Earth system models (ESMs) from phase 5 of the Coupled Model Intercomparison Project (CMIP5) were evaluated, focusing on the seasonal sensitivities of net biome production (NBP), net primary production (NPP), and heterotrophic respiration (Rh) to interannual variations in temperature and precipitation during 1982-2005 and their changes over the twenty-first century. Temperature sensitivity of NPP in ESMs was generally consistent across northern high-latitude biomes but significantly more negative for tropical and subtropical biomes relative to satellite-derived estimates. The temperature sensitivity of NBP in both inversion-based and ESM estimates was generally consistent in March-May (MAM) and September-November (SON) for tropical forests, semiarid ecosystems, and boreal forests. By contrast, for inversion-based NBP estimates, temperature sensitivity of NBP was nonsignificant for June-August (JJA) for all biomes except boreal forest; whereas, for ESM NBP estimates, the temperature sensitivity for JJA was significantly negative for all biomes except shrublands and subarctic ecosystems. Both satellite-derivedNPP and inversion-based NBP are often decoupled from precipitation, whereas ESM NPP and NBP estimates are generally positively correlated with precipitation, suggesting that ESMs are oversensitive to precipitation. Over the twenty-first century, changes in temperature sensitivities of NPP, Rh, and NBP are consistent across all RCPs but stronger under more intensive scenarios. The temperature sensitivity of NBP was found to decrease in tropics and subtropics and increase in northern high latitudes in MAM due to an increased temperature sensitivity of NPP. Across all biomes, projected temperature sensitivity of NPP decreased in JJA and SON. Projected precipitation sensitivity of NBP did not change across biomes, except over grasslands in MAM.

No description available.

The dynamics of spin and mechanical displacement in ionic materials are independently well understood. This abstract work aims to investigate the spin and the displacement dynamics of two ions, while including coupled interactions between the ions’ spin and displacement. I have found that the dynamics depend on the speed of both the spin and the displacement. / Joners spinn- respektive förflyttningsdynamik har väl etablerade teoretiska grunder. Med syftet att utöka den teoretiska kunskapen undersöker jag hur spinn- och förflyttningsdynamiken hos två joner påverkas av kopplade interaktioner mellan deras spinn och förflyttning. Resultatet visar att dynamiken beror på spinnets och förflyttningens farter.

No description available.

published_or_final_version / abstract / Chemistry / Master / Master of Philosophy

published_or_final_version / Statistics and Actuarial Science / Master / Master of Philosophy

No description available.

No description available.

The hypothalamic peptide arginine vasopressin (AVP) is an important regulator of adrenocorticotropin (ACTH) release from the anterior pituitary. AVP stimulates ACTH secretion from corticotroph cells by activating the pituitary vasopressin receptor (V1b-R), a member of the G protein-coupled receptor (GPCR) family. In vitro, repeated stimulus of anterior pituitary cells with AVP results in rapid desensitisation. The aim of this research was to develop methods needed to use RNA interference (RNAi) to investigate the role of G protein-coupled receptor kinase 5 (GRK5) in this desensitisation process. This required the development of a model system using human embryonic kidney (HEK) 293 cells transfected with the pituitary vasopressin receptor, V1b-R. AVP binding to the V1bR activates the phosphoinositide signalling pathway, leading to production of inositol phosphates (IPs), which can be measured following radiolabelling of cells with myo-[³H]inositol. Stimulation of V1b-R-transfected cells for 15 min with AVP (100nM) increased IP production to 235.5 ± 23.4 % (n=3, p<0.02) of that seen in un-stimulated control cells. Following a 5 minute pre-treatment with 5nM VP, the IP response to stimulation with 100nM VP for 15 min was reduced to 62.8 ± 9.1 % (n=4, p<0.02) of that seen in control cells that were not pre-treated. These data indicate that AVP-desensitisation can be induced and measured in V1bR-transfected HEK293 cells following a brief pre-treatment with a physiological concentration of AVP. This model system will enable RNAi to be used to investigate the role of GRK5 in AVP-desensitisation. When using RNAi, it is essential to establish that the effects observed are the result of small interfering RNA (siRNA) specific degradation of the target mRNA. Quantitative reverse transcription PCR (qRT-PCR) was used to measure the expression of GRK5 at the mRNA level in HEK293 cells. Human GRK5 mRNA was amplified using qRT-PCR with GRK5 specific primers, providing confirmation that GRK5 is expressed endogenously in HEK293 cells. GRK5 expression studies were carried out to evaluate whether the qRT-PCR methods developed would be suitable to measure knockdown of GRK5 mRNA using RNAi. These experiments were also designed to assess the impact of HEK293 cell culture methods on expression of GRK5. Expression of GRK5 did not vary with passage number (2-26 passages). The GRK5 expression in HEK293 cells that were maintained in culture for 5 days (grown to a confluence of approximately 100%) was 7.4 ± 0.9 fold greater (n=2, p<0.05) than for cells cultured for 3 days (grown to a confluence of approximately 65%). These data indicate that GRK5 expression is affected by HEK293 culture conditions. Furthermore, the results demonstrated that a significant difference in GRK5 expression could be measured in HEK293 cells using qRT-PCR. Therefore the results reported in this thesis provide the basis for future studies utilising RNAi to investigate mechanisms underlying V1b-R desensitisation.

In the past decade, charge-coupled devices (CCDs) have rapidly become the astronomical imaging detector of choice for the visible and near-IR spectral regions. There are, however, several problems which have greatly reduced the availability of sufficient quality CCDs to the astronomical community. These include the low blue and ultraviolet quantum efficiency of thick devices, the lack of properly thinned devices, warped imaging surfaces, interference fringing, and the small size of the detectors themselves compared to telescope focal planes. This dissertation presents methods which can be used to optimize CCDs obtained from various manufacturers for astronomical observations. A new thinning technique which produces an optically flat surface across an entire CCD is demonstrated. A mounting technique which maintains a flat and stable imaging surface for thinned devices by bonding the CCD backside against a transparent glass support substrate is also demonstrated. Bump bonding of CCDs onto a silicon support before thinning is discussed as a future mounting/thinning technique. The design of antireflection coatings for the near-UV through near-IR spectral regions is explained and demonstrated on silicon diodes, allowing quantum efficiencies as high as 90% to be obtained. The reduction of interference fringing amplitudes by as much as 70% in the red and near-IR with AR coatings is also discussed. And finally, the design of CCD focal plane mosaics using the optimization techniques presented is discussed.