published_or_final_version / Botany / Doctoral / Doctor of Philosophy

Agrobacterium tumefaciens is a gram-negative soil bacterium that causes crown gall tumors on dicotyledenous plants. The transferred DNA (T-DNA) portion of the A. tumefaciens tumor-inducing (Ti) plasmid enters infected plant cells and integrates into plant nuclear DNA. The T-DNA is accompanied into plant cells by the VirD2 endonuclease covalently attached to its 5' end. VirE2, a cooperative, single-stranded DNA-binding protein is also transported into plant cells during infection by A. tumefaciens. VirD2 and VirE2 contain nuclear localization signals (NLSs) and are transported into the plant cell nucleus. The location of functional domains by the insertion of Xhol linker oligonucleotides throughout virE2 is reported. A ssDNA binding domain was located in the C-terminal half of VirE2, as well two domains involved in cooperative single-stranded DNA binding. Further, we isolated a mutation in the central region of VirE2 that decreased tumorigenicity, but did not affect ssDNA binding. / Graduation date: 1997

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Typescript (photocopy) / vi, 115 leaves, [25] leaves of plates : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Plant Pathology, 1982

Typescript (photocopy) / vi, 115 leaves, [25] leaves of plates : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Plant Pathology, 1982

Thesis (Ph. D.)--University of Adelaide, Dept. of Crop Protection, 1997. / Copy of author's previously published work inserted. Includes bibliographical references (leaves 114-136).

Thesis (Ph. D.)--University of Wisconsin--Madison, 1940. / Typescript. Includes abstract and vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 45-47).

Thesis (Ph. D.)--University of Hong Kong, 2000. / Includes bibliographical references (leaves 180-212).

Thesis (Ph. D.)--University of Wisconsin-Madison, 1941. / Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

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