The International Space Station provides a high-quality of microgravity and extended exposure time which makes it a platform of choice for microgravity research. In order to increase accessibility of onboard experimentation, Space Applications Services will soon launch the ICE Cubes facility as part of its ICE Cubes Service. The facility is foreseen to host standardized plug-and-play payload cubes to reduce overall cost and procedure time required to install payloads on the station. To remotely support the facility it is decided to develop a utility cube named Cube-Zero that will be launched and installed with the facility on the station. This thesis work included the complete design, development and documentation of the cube. The thesis started by conducting a preliminary needs and market study from which two specific purposes were defined for the cube. In addition to its original function of support-utility, the cube is tasked to be a technical commercial demonstrator for the service. This led to the conceptual design of the cube as a multidisciplinary framework able to host two user-defined experiment modules. The preliminary concept was further refined in this paper and with support of prototypes, simulations and analyses led to a final functional design for the Cube-Zero. The work is concluded with the manufacturing of an engineering model of the cube. The model is fully operational, can support the test of the facility before launch and can demonstrate to users its versatility and ease of use in operating any kind of experiment module. Eventually, the information gathered in this thesis report will support future users into developing their own Cube-Zero payload module and guide Space Applications Services into manufacturing, testing and operating the Cube-Zero protoflight model. / ICE Cubes