Internet Protocol version 6 (IPv6) is being adopted in networks around the world as the Internet Protocol version 4 (IPv4) addressing space reaches its maximum capacity. Although there are IPv6 applications being developed, there are not many production IPv6 networks in place in which these applications can be deployed. Simulation presents a cost effective alternative to setting up a live test bed of devices to validate specific IPv6 environments before actual physical deployment. OPNET Modeler provides the capability to simulate the IPv6 protocol and System-in-the-Loop, an add-on module, allows for real communication traffic from physical devices to be converted and sent over the simulated network. This research has developed a campus framework, modeled after the Virginia Tech Blacksburg campus, to verify and validate standard and custom IPv6 applications. Specifically, the framework was used to test MT6D, a custom IPv6 security application developed in the Virginia Tech IT Security Lab (ITSL) as well as test Voice over IP (VoIP) as a somewhat bandwidth demanding benchmarking standard application. The work presented shows that simulation helped to identify potential issues within the applications and verified the results after fixes were applied. It also reveals challenges and shortcomings of OPNET Modeler's IPv6 implementation and presents potential solutions to these problems. / Master of Science