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1	Molecular mechanisms regulating the epithelial barrier : key roles for Cx26 and ADAM17 during bacterial infection Simpson, Charlotte Louise January 2015 (has links) This study investigated how gastrointestinal and skin bacterial infections were affected by differential expression of connexin (Cx) 26 and a disintegrin and metalloprotease (ADAM) 17 in vitro. Cx26 is a component of gap junctions, which facilitate the transfer of small molecules between two cells. Recessive mutations in Cx26 cause non syndromic hearing loss (NSHL), and in certain populations, specific mutations account for the majority of Cx26 related NSHL. Their common occurrence suggests that they may provide a heterozygous, protective advantage to carriers. In this study adherence by the attaching and effacing pathogen Enteropathogenic Escherichia coli (EPEC) was significantly reduced in cells expressing mutant Cx26 compared to wild type Cx26. Furthermore, EPEC adherence and invasion of an alternative enteric pathogen, Shigella flexneri were reduced following treatment with Cx26 short-interfering-RNA in intestinal cells. These findings suggest that the loss of functional Cx26 expression improves protection against enteric bacteria. ADAM17 releases substrates including tumour necrosis factor alpha and ligands of the epidermal growth factor receptor and therefore is involved in the induction of immune responses and maintenance of the epidermal barrier. This study demonstrated that ADAM17 provides protection during Staphylococcus aureus infection of keratinocytes. Subsequently the protective effects of ADAM17 mediated protection were explored. Secretion of the proinflammatory cytokines Interleukins 6 and 8 correlated with ADAM17 activity. Additionally gene expression profiling was performed which identified the IL-17 signalling pathway, which is known to be important during S. aureus infection, as a potential downstream target of ADAM17. In summary, based on these findings, Cx26 and ADAM17 may represent potential therapeutic targets for gastrointestinal and skin bacterial pathogens. 616.9
2	"Eine vergleichende Genexpressionsanalyse von Gap- Junction- Strukturproteinen in oralen Plattenepithelkarzinomen und gesunder Schleimhaut" / A comparative gene expression study of gap-junction proteins in oral squamous cell carcinomas and normal mucosa Brodmann, Tobias 23 April 2012 (has links) No description available. 610 Medizin, Gesundheit Medicine Connexin Connexine Gap Junction oral;Plattenepithelkarzinom PEC Genexpression Expression Karzinogenese GJIC Cx 45 Cx 26 Connexin 45 Connexin 26 connexin connexins gap junction GJIC Cx 45 Cx 26 Cx-45 Cx-26 oral squamous cell carcinoma carcinogenesis OSCC gene expression comparative gene expression 44.81 MED 000: Medizin

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Molecular mechanisms regulating the epithelial barrier : key roles for Cx26 and ADAM17 during bacterial infection

"Eine vergleichende Genexpressionsanalyse von Gap- Junction- Strukturproteinen in oralen Plattenepithelkarzinomen und gesunder Schleimhaut" / A comparative gene expression study of gap-junction proteins in oral squamous cell carcinomas and normal mucosa