• Refine Query
  • Source
  • Publication year
  • to
  • Language
  • 50
  • 3
  • 2
  • 1
  • 1
  • Tagged with
  • 80
  • 80
  • 38
  • 21
  • 17
  • 15
  • 15
  • 14
  • 13
  • 11
  • 11
  • 11
  • 11
  • 11
  • 10
  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
51

Rational design of DNA-based lipid membrane pores

Göpfrich, Kerstin January 2017 (has links)
DNA nanotechnology has revolutionised our capability to shape and control three-dimensional structures at sub-nanometre length scales. In this thesis, we use DNA to build synthetic membrane-inserting channels. Porphyrin and cholesterol tags serve as membrane anchors to facilitate insertion into the lipid membrane. With atomic force microscopy, confocal imaging and ionic current recordings we characterise our DNA nanochannels that mimic their natural protein-based counterparts in form and function. We find that they exhibit voltage-dependent conductance states. Amongst other architectures, we create the largest man-made pore in a lipid membrane to date approaching the electrical diameter of the nuclear pore complex. Pushing the boundaries on the other end of the spectrum, we demonstrate the ultimately smallest DNA membrane pore made from a single membrane-spanning DNA duplex. Thereby, we proof that ion conduction across lipid membranes does not always require a physical channel. With experiments and MD simulations we show that ions flow through a toroidal pore emerging at the DNA-lipid interface around the duplex. Our DNA pores spanning two orders of magnitude in conductance and molecular weight showcase the rational design of synthetic channels inspired by the diversity of nature - from ion channels to porins.
52

Quadruplexes de guanines : formation, stabilité et interaction / Guanine quadruplexes : formation, stability and interaction

Tran, Phong Lan Thao 09 December 2011 (has links)
Les quadruplexes de guanines (G4) sont des structures non canonique d’acides nucléiques à quatre brins formées à partir de séquences ADN ou ARN riches en guanines. Ces structures reposant sur la formation et l’empilement de quartets de guanines sont très polymorphes, leur formation pourrait être envisagé dans de nombreux domaines d’application, aussi bien pour les biotechnologies que les nanotechnologies. L’étude de G4 tétramoléculaires modifiés présentée dans ce manuscrit a participé à la compréhension du mécanisme d’association de ces complexes. En particulier, nous avons montré que l’insertion de 8-méthyle-2’-déoxyguanosine à l’extrémité 5’ de la séquence favorise l’association et la stabilité du G4. Par ailleurs, l’étude de l’ADN en série L (image de l’ADN naturel dans un miroir) a montré la formation d’un G4 tétramoléculaire avec les mêmes propriétés que son énantiomère, à l’exception de sa chiralité, qui est inversée. L’étude a révélé également une auto-exclusion de deux énantiomères (forme D et forme L) démontrant un assemblage contrôlé des brins parallèles. Ce travail de thèse a aussi permis d’introduire un système simple et stable de visualisation de G4 tétramoléculaire antiparallèle, appelé “ADN synaptique”, sur une nanostructure d’ADN origami. In vivo, ces structures pourraient être impliquées de façon transitoire dans de nombreux processus biologiques, en particulier au niveau des télomères. Nous avons réalisé, au cours de cette thèse, une étude comparative de la structure et de la stabilité des séquences télomériques connues de différents organismes. Cette étude a permis d’enrichir les données nécessaires au développement d’un algorithme prédisant la stabilité de G4. Enfin, nous avons développé une méthode facile et peu coûteuse de criblage (G4-FID) sur plaques 96 puits permettant d’identifier l’interaction de ligands avec différentes séquences biologiques pertinentes. La stabilisation du G4 dans certaines régions du génome via des ligands spécifiques pourrait limiter la prolifération de cellules tumorales et est donc intéressante pour les thérapies anticancéreuses. / Guanine quadruplexes (G4) are non-canonical four-stranded nucleic acid structures formed by guanine-rich DNA and RNA sequences. Theses polymorphic structures are built from the stacking of several G-quartets and could be involved in many fields, in biotechnology as well as in nanotechnology. The study of modified tetramolecular G4 presented in this manuscript participated to the understanding of tetramolecular G4 formation. Especially, we showed that the insertion of 8-methyl-2’-deoxyguanosine at the 5’-end of the sequence accelerate G4 formation and increase its stability. Besides, we demonstrate here that short guanine rich L-DNA strands (mirror image of natural DNA) form a tetramolecular G4 with the same properties than their enantiomer, but with opposite chirality. The study revealed also self-exclusion between two enantiomers (D- and L- form), showing the controlled parallel self-assembly of different G-rich strands. This work introduced also a simple and stable system to observe tetramolecular antiparallel G4 formation, called “synaptic DNA”, into a DNA origami nanostructure. In vivo, such structures appear to be implicated in genome dynamics, and especially at telomeres. During this thesis, we dedicated a study to the comparison of G4 folding and stability of known telomeric sequences from different organisms. The present study allowed enriching the dataset necessary to build and refine algorithms predicting G4 stability. Last but not least, we developed a G4 ligand screening method onto 96-well plates allowing the comparison of different biological relevant sequences. The G4 stabilisation by specific ligands in some genome regions may prevent cancer cell proliferation, making it an attractive target for anticancer therapy.
53

Facile Fabrication of Meso-to-Macroscale Single-Molecule Arrays for High-Throughput Digital Assays

January 2019 (has links)
abstract: One of the single-most insightful, and visionary talks of the 20th century, “There’s plenty of room at the bottom,” by Dr. Richard Feynman, represented a first foray into the micro- and nano-worlds of biology and chemistry with the intention of direct manipulation of their individual components. Even so, for decades there has existed a gulf between the bottom-up molecular worlds of biology and chemistry, and the top-down world of nanofabrication. Creating single molecule nanoarrays at the limit of diffraction could incentivize a paradigm shift for experimental assays. However, such arrays have been nearly impossible to fabricate since current nanofabrication tools lack the resolution required for precise single-molecule spatial manipulation. What if there existed a molecule which could act as a bridge between these top-down and bottom-up worlds? At ~100-nm, a DNA origami macromolecule represents one such bridge, acting as a breadboard for the decoration of single molecules with 3-5 nm resolution. It relies on the programmed self-assembly of a long, scaffold strand into arbitrary 2D or 3D structures guided via approximately two hundred, short, staple strands. Once synthesized, this nanostructure falls in the spatial manipulation regime of a nanofabrication tool such as electron-beam lithography (EBL), facilitating its high efficiency immobilization in predetermined binding sites on an experimentally relevant substrate. This placement technology, however, is expensive and requires specialized training, thereby limiting accessibility. The work described here introduces a method for bench-top, cleanroom/lithography-free, DNA origami placement in meso-to-macro-scale grids using tunable colloidal nanosphere masks, and organosilane-based surface chemistry modification. Bench-top DNA origami placement is the first demonstration of its kind which facilitates precision placement of single molecules with high efficiency in diffraction-limited sites at a cost of $1/chip. The comprehensive characterization of this technique, and its application as a robust platform for high-throughput biophysics and digital counting of biomarkers through enzyme-free amplification are elucidated here. Furthermore, this technique can serve as a template for the bottom-up fabrication of invaluable biophysical tools such as zero mode waveguides, making them significantly cheaper and more accessible to the scientific community. This platform has the potential to democratize high-throughput single molecule experiments in laboratories worldwide. / Dissertation/Thesis / Doctoral Dissertation Biomedical Engineering 2019
54

Arranging multiple types of enzymes in defined space by modular adaptors / モジュール型アダプターを利用した複数酵素の特異的空間配置

NGUYEN, MINH THANG 25 March 2019 (has links)
京都大学 / 0048 / 新制・課程博士 / 博士(エネルギー科学) / 甲第21886号 / エネ博第387号 / 新制||エネ||75(附属図書館) / 京都大学大学院エネルギー科学研究科エネルギー基礎科学専攻 / (主査)教授 森井 孝, 教授 木下 正弘, 教授 片平 正人 / 学位規則第4条第1項該当 / Doctor of Energy Science / Kyoto University / DGAM
55

Tunable Nanocalipers to Probe Structure and Dynamics in Chromatin

Le, Jenny Vi, Le January 2018 (has links)
No description available.
56

Chemical Templating by AFM Tip-Directed Nano-Electrochemical Patterning

Nelson, Kyle A. 14 December 2011 (has links) (PDF)
This work has examines the creation and use of chemical templates for nanocircuit and other nanodevice fabrication. Chemical templating can be useful in attachment, orientation and wiring of molecularly templated circuits. DNA origami provides a suitable method for creating molecularly templated circuits as DNA can be folded into complex shapes and functionalized with active circuit elements, such as semiconducting nanomaterials. Surface attachment of DNA origami structures can be accomplished by hybridization of dangling single-stranded DNA (ssDNA) on the origami structures with complementary surface-bound strands. Chemical templating provides a pathway for placing the patterned surface-bound attachment points needed for surface alignment of the molecular templates. Chemical templates can also be used to connect circuit elements on the surface by selectively metallizing the templates to form local wiring. AFM tip-directed nano-oxidation was selected as the method for patterning to create chemical templates. This project demonstrates new techniques for creating, continuous metallization of, and DNA attachment to nanochemical templates. Selective-continuous metallization of nanochemical templates is needed for wiring of circuit templates. To improve the metallization density and enable the continuous nano-scale metallization of amine-coated surfaces, the treatment of amine-coated surfaces with a plating additive prior to metallization was studied. The additive treatment resulted in a 73% increase in seed material, enabling continuous nano-scale metallization. A new method was developed to create amine nanotemplates by selective attachment of a polymer to surface oxide patterns created by nano-oxidation. The treatment of the templates with the additive enabled a five-fold reduction in feasible width for continuous metallization. Nano-oxidation was also used in the nanometer-scale patterning of a thiol-coated surface. Metallization of the background thiols but not the oxidized patterns resulted in a metal film that was a negative of the patterns. The resulting metal film may be useful for nanometer-scale pattern transfer. DNA-coated gold nanoparticles (AuNPs) were selectively attached to amine templates by an ionic interaction between the template and ssDNA attached to the particles. Only the ssDNA on the bottom of the AuNPs interacted with the template, leaving the top strands free to bind with complementary ssDNA. Attempts to attach origami structures to these particles were only marginally successful, and may have been hindered by the presence of complementary ssDNA in solution but not attached to the origami, or the by the low density of DNA-AuNPs attached to the templates. The formation of patterned binding sites by direct, covalent attachment of ssDNA to chemical templates was also explored. Initial results indicated that ssDNA was chemically bound to the templates and able to selectively bind to complementary strands; however, the observed attachment density was low and further optimization is required. Methods such as these are needed to enable nano-scale, site-specific alignment of nanomaterials.
57

Metallization of DNA and DNA Origami Using a Pd Seeding Method

Geng, Yanli 15 January 2013 (has links) (PDF)
In this dissertation, I developed a Pd seeding method in association with electroless plating, to successfully metallize both lambda DNA and DNA origami templates on different surfaces. On mica surfaces, this method offered a fast, simple process, and the ability to obtain a relatively high yield of metallized DNA nanostructures. When using lambda DNA as the templates, I studied the effect of Pd(II) activation time on the seed height and density, and an optimal activation time between 10 and 30 min was obtained. Based on the Pd seeds formed on DNA, as well as a Pd electroless plating solution, continuous Pd nanowires that had an average diameter of ~28 nm were formed with good selectivity on lambda DNA. The selected Pd activation time was also applied to metallize "T"-shape DNA origami, and Au coated branched nanostructures with a length between 200-250 nm, and wire diameters of ~40 nm were also fabricated. In addition, I found that the addition of Mg2+ ion into the reducing agent and electroless plating solution could benefit the surface retention of Pd seeded DNA and Au plated DNA structures. This work indicated that DNA molecules were promising templates to fabricate metal nanostructures; moreover, the formation of Au metallized branched nanostructures showed progress towards nanodevice fabrication using DNA origami. Silicon surfaces were also used as the substrates for DNA metallization. More complex circular circuit DNA origami templates were used. To obtain high enough seed density, multiple Pd seeding steps were applied which showed good selectivity and the seeded DNA origami remained on the surface after seeding steps. I used distribution analysis of seed height to study the effect of seeding steps on both average height and the uniformity of the Pd seeds. Four-repeated palladium seedings were confirmed to be optimal by the AFM images, seed height distribution analysis, and Au electroless plating results. Both Au and Cu metallized circular circuit design DNA origami were successfully obtained with high yield and good selectivity. The structures were maintained well after metallization, and the average diameters of Au and Cu samples were ~32 nm and 40 nm, respectively. Electrical conductivity measurements were done on these Au and Cu samples, both of which showed ohmic behavior. This is the first work to demonstrate the conductivity of Cu metallized DNA templates. In addition, the resistivities were calculated based on the measured resistance and the size of the metallized structures. My work shows promising progress with metallized DNA and DNA origami templates. The resulting metal nanostructures may find use as conducting interconnects for nanoscale objects as well as in surface enhanced Raman scattering analysis.
58

Control of Dynamic DNA Origami Mechanisms Using Integrated Functional Components

Miller, Carl A. 20 May 2015 (has links)
No description available.
59

FOLDING DYNAMICS OF G-QUADRUPLEXES DURING TRANSCRIPTION AND IN A NANO-CONFINEMENT

Shrestha, Prakash 02 January 2018 (has links)
No description available.
60

Nucleosome Regulation of Transcription Factor Binding Dynamics: a Single-molecule Study

Luo, Yi January 2015 (has links)
No description available.

Page generated in 0.0331 seconds