No description available.

Thesis (M.A.)--Wheaton College Graduate School, 2002. / Bibliography: leaves 172-184.

Thesis (M.A.)--Wheaton College Graduate School, 2002. / Abstract. Includes bibliographical references (leaves 172-184).

Thesis (M.A.)--Wheaton College Graduate School, 2002. / Abstract. Includes bibliographical references (leaves 172-184).

Thesis (M.A.)--Wheaton College Graduate School, 2002. / Abstract. Includes bibliographical references (leaves 172-184).

No description available.

Thesis (M.A.)--Wheaton College Graduate School, 2002. / Includes bibliographical references (leaves 172-184).

Thesis (M.A.)--Briercrest Biblical Seminary, 1999. / Abstract. Includes bibliographical references (leaves 96-99).

Made available in DSpace on 2016-08-03T12:21:00Z (GMT). No. of bitstreams: 1 Andrea.pdf: 2250624 bytes, checksum: 33dd44c7d63479272ceb3b537a60e4a9 (MD5) Previous issue date: 2010-03-03 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / The belief in the rapture of the church is part of a fundamentalist eschatological system that is often called premillennial dispensationalism. Its appearance is noted to start in the XIX century through the teachings of Jonh Nelson Darby, a british evangelical preacher founder of the Plymouth Brethren. His teaching incompass the coming of Christ in two steps. One in secret for the church, taking it to heaven and saving it from seven years of tribulation that will follow, the second, a glorious return at the end of seven years for establishment the millennial kingdom on earth, the teaching of Darby were popularized in the footnotes of the Scofield Reference Bible published in 1909 by Cyrus I. Scofield, and it is still set in the eschatological beliefs of the majority of the evangelical fundamentalist churches, both in the EUA and Brazil. In 2002, the film was produced: Left Behind for portraying this belief as well as its update to recent times. However, further study of this belief exposes its doctrinal construct character in which biblical texts from different perspectives of the old and New Testaments are united to form an eschatological framework about to be fulfilled.(AU) / A crença no arrebatamento da Igreja faz parte de um sistema escatológico fundamentalista que costuma ser chamado de dispensacionalismo pré-milenista. Seu surgimento se dá a partir do século XIX, pelo ensino de John Nelson Darby, um pregador evangélico britânico, fundador dos Irmãos de Plymouth. Seu ensino aguarda a vinda de Cristo em duas etapas: uma, em secreto para a Igreja, há de levá-la ao Céu e poupá-la dos sete anos de tribulação que se seguirão; e outra, num aparecimento glorioso, ao final dos sete anos há de instaurar o reino milenial sobre a terra. O ensino de Darby foi popularizado nas notas de rodapé da Bíblia de Referência Scofield, publicada em 1909 por Cyrus I. Scofield, e ainda hoje se configura na crença escatológica da maioria das igrejas evangélicas fundamentalistas, tanto nos EUA quanto no Brasil. Em 2002 foi produzido o filme: Deixados para Trás que retrata esta crença bem como sua atualização para épocas recentes. Contudo, um estudo mais aprofundado desta crença expõe seu caráter de construto doutrinário, em que textos bíblicos de perspectivas diferentes, do Antigo e do Novo Testamento, são unidos para formar um quadro escatológico em vias de se cumprir.(AU)

Environmental chemicals may affect human health by disrupting endocrine function. Many of the endocrine disrupting chemicals (EDCs) are estrogens or estrogen-like molecules that have been classified as environmental estrogens or xenoestrogens (XEs). XEs include endosulfan, chlordance, nonylphenol, bisphenol A, octylphenol, and coumestrol, etc. Although these compounds have wide structural diversity, but all have in common the and/or other hydrophobic components. Many studies have shown that XEs affect cell viability. For instance, Nonylphenol is used in surfactants or plasticizers and bisphenol A (4, 4¡¦-isopropylidene-2-diphenol) is used as protective coatings on food containers and for composites and sealants in dentistry. Most previous studies have focused on the toxicity of XEs on development process and reproductive system, especially in aquatic ecosystems. Thus, the effects of these two environmental chemicals on the toxicological effect are still controversial. The aim of this study is to investigate the molecular mechanisms of nonylphenol and bisphenol A in induction of cell death in human gastric cancer (SCM-1) cells and Madin Darby canine renal tubular (MDCK) cells. First, WST-1 reduction assays and propidium iodide-staining assay were used to determine cell viability and apoptosis in the present of nonylphenol and bisphenol A. Furthermore, we will use immunoblotting to measure the activity of apoptotic markers caspase-3, mitogen-activated protein kinases (MAPKs) to survey how nonylphenol affects apoptotic pathways. Besides, I will explore bisphenol A whether induces cell death and the mechanisms underlying the [Ca2+]i rise in MDCK cells. The results may be helpful for understanding the pharmacological and toxicological effects of these two environmental chemicals in cells from important organs. Results showed that nonylphenol caused apoptosis via the activation of caspase-3 in cultured human gastric cancer (SCM-1) cells. Although nonylphenol could activate the phosphorylation of extracellular signal-regulated kinase (ERK), c-Jun NH2-terminal kinase (JNK) and p38 mitogen-activated protein kinase (p38 MAPK), only SB203580 (a p38 MAPK inhibitor) partially prevented cells from apoptosis. Nonylphenol was also found to induce [Ca2+]i increases and pretreatment with BAPTA/AM, a Ca2+ chelator, prevented nonylphenol-induced [Ca2+]i increases, and protect cells from death. These results suggest that nonylphenol induced apoptosis via a Ca2+- and p38 MAPK-dependent pathway. On the other hand, the effect of the environmental contaminant bisphenol A on cytosolic free Ca2+ concentrations ([Ca2+]i) in Madin Darby canine kidney (MDCK) cells is unclear. This study explored whether bisphenol A changed basal [Ca2+]i levels in suspended MDCK cells by using fura-2 as a Ca2+-sensitive fluorescent dye. Bisphenol A at concentrations between 50-300 £gM increased [Ca2+]i in a concentration-dependent manner. The Ca2+ signal was reduced partly by removing extracellular Ca2+. Bisphenol A induced Mn2+ influx, leading to quench of fura-2 fluorescence suggesting Ca2+ influx. This Ca2+ influx was inhibited by phospholiapase A2 inhibitor aristolochic acid, store-operated Ca2+ channel blockers nifedipine and SK&F96365; and protein kinase C inhibitor GF109203X. In Ca2+-free medium, pretreatment with the mitochondrial uncoupler carbonylcyanide m-chlorophenylhydrazone (CCCP) and the endoplasmic reticulum Ca2+ pump inhibitors thapsigargin or 2,5-di-tert-butylhydroquinone (BHQ) inhibited bisphenol A-induced Ca2+ release. Conversely, pretreatment with bisphenol A abolished thapsigargin (or BHQ)- and CCCP-induced [Ca2+]i rise. Inhibition of phospholipase C with U73122 abolished bisphenol-induced [Ca2+]i rise. Bisphenol A caused concentration-dependent decrease in cell viability via apoptosis in a Ca2+-independent manner. Collectively, in MDCK cells, bisphenol A induced [Ca2+]i rises by causing phospholipase C-dependent Ca2+ release from the endoplasmic reticulum and mitochondria and Ca2+ influx via phospholipase A2-protein kinase C-sensitive store-operated Ca2+ channels. Key words: calcium, apoptosis, human gastric cancer cells (SCM-1), Madin Darby canine kidney (MDCK), nonylphenol, bisphenol A.