Indiana University-Purdue University Indianapolis (IUPUI)

Indiana University-Purdue University Indianapolis (IUPUI)

Indiana University-Purdue University Indianapolis (IUPUI) / A study was made to develop a method for investigating the quantity and location of vascular leakage during acute inflammation of the dental pulp. Use was made of the method of "vascular labelling" by which carbon particles placed in the blood are deposited in the walls of leaking vessels. Three dogs were used, with cavities of varying depth being cut in their permanent teeth. Vascular leakage was induced in the skin and in some of the teeth by applying histamine phosphate solution. The tissues were studied in normal histologic sections and as cleared specimens. It was found that the number of leaking vessels was much less in the dental pulp than in the skin and that leakage was confined to the area immediately below the cavity preparation unless histamine had been applied to the cavity, when the labelled vessels were found further apically. The number of leaking vessels depended on the depth of the cavity, becoming greater as the cavity depth increased. These findings would support the common finding that the degree of inflammation beneath cavity preparations increases with cavity depth. The conclusion was that the method developed held promise as a tool in investigating vascular changes in the dental pulp.

Indiana University-Purdue University Indianapolis (IUPUI) / This was a histologic study of continued apical development in the pulpless permanent teeth of dogs. The vital pulps were exposed to the oral fluids for one week. The root canals were filled with either calcium hydroxide and camphorated parachlorophenol (CMCP) or calcium hydroxide and distilled water. A vital dye, Procion red was injected to demonstrate the formation of calcified tissue. After four months, the animals were sacrificed. Decalcified semi-serial sections were studied. In 31 of 61 pulpless permanent teeth, with incompletely developed roots, continued apical development occurred. Apical calcified tissue resembling cementum was observed in 55 per cent of the specimens treated with calcium hydroxide and CMCP and in 42 per cent of those, with calcium hydroxide and distilled water. Complete closure of the apical foramen was observed in 29 and 11 per cent of the specimens respectively. Inflammation of the periapical tissues was present in 48 per cent of the calcium hydroxide and CMCP group and in 79 per cent of the other group. A significant association was found between the degree of inflammation and apical closure, (P<.001) for the calcium hydroxide-CMCP group, (P<.005) for the calcium hydroxide-distilled water group. The results suggest that calcium hydroxide and CMCP was superior to calcium hydroxide and distilled water and that the addition of CMCP to the paste reduced inflammation. Procion dye was shown to be an effective in vivo dental hard tissue marker.

Indiana University-Purdue University Indianapolis (IUPUI) / The purpose of this investigation was to evaluate the association between the depth of a carious lesion and the sclerotic dentin deposited beneath a calcium hydroxide methyl cellulose base material. The sample chosen consisted of teeth with deep caries and possible pulp exposure, as evidenced by a critical radiographic examination. Clinical procedures consisted of a preoperative serial radiograph, followed by complete caries removal. A barium sulphate radiopaque solution was then applied to the base of the preparation, followed by a second serial radiograph. The barium sulphate was removed and a calcium hydroxide methyl cellulose base was applied and the tooth restored with a silver amalgam alloy. Subsequently one, three, six, and nine month serial radiographs were taken postoperatively. Calcification change of sclerotic dentin overlying the pulp was measured in relation to pulpal floor width by the television instrumentation. The conclusions of this study are as follows: 1. A calcium hydroxide methyl cellulose base material acts as a "trigger mechanism," in deep cavities, stimulating the deposition of sclerotic dentin. 2. The thinner the initial pre-restored pulpal floor, the more rapid and dramatic is the postoperative pulpal floor increase in width, which is apparently a protective pulpal response. 3. During the periods of time when a significant increase in pulpal floor width was noted, the percent change in calcification of this area decreased. Conversely, when little pulpal floor width increase occurred, the percent change in calcification increased.

Thesis (M.Sc.D.)--Boston University School of Graduate Dentistry, June 1967. / 168 pages plates 29 cm.

Thesis (M.S.)--University of Michigan, 1966.

Thesis (M.S.)--University of Michigan, 1972.

Thesis (M.S.)--University of Michigan, 1982.

Thesis (M.S.)--University of Michigan, 1983.