Thesis (M.S.D.)--Indiana University School of Dentistry, 2009. / Title from PDF t. p. (viewed Feb. 9, 2009) Advisor(s): Jeffrey A. Platt, Chair of the Research Committee, Tien-Min Gabriel Chu, Michael A. Cochran, Bruce A. Matis, Burak Taskonak, B. Keith Moore. Curriculum vitae. Includes abstract. Includes bibliographical references (leaves 71-76).

Submitted by Inês Marinho (bele_ballet@hotmail.com) on 2016-06-24T13:59:54Z No. of bitstreams: 1 Dissertação - Kleyver do Nascimento Sobrinho.pdf: 1970241 bytes, checksum: 9563817a411ce59d3b1fe44712e1f864 (MD5) / Approved for entry into archive by Divisão de Documentação/BC Biblioteca Central (ddbc@ufam.edu.br) on 2016-07-05T16:05:44Z (GMT) No. of bitstreams: 1 Dissertação - Kleyver do Nascimento Sobrinho.pdf: 1970241 bytes, checksum: 9563817a411ce59d3b1fe44712e1f864 (MD5) / Approved for entry into archive by Divisão de Documentação/BC Biblioteca Central (ddbc@ufam.edu.br) on 2016-07-05T16:08:27Z (GMT) No. of bitstreams: 1 Dissertação - Kleyver do Nascimento Sobrinho.pdf: 1970241 bytes, checksum: 9563817a411ce59d3b1fe44712e1f864 (MD5) / Made available in DSpace on 2016-07-05T16:08:27Z (GMT). No. of bitstreams: 1 Dissertação - Kleyver do Nascimento Sobrinho.pdf: 1970241 bytes, checksum: 9563817a411ce59d3b1fe44712e1f864 (MD5) Previous issue date: 2016-02-25 / FAPEAM - Fundação de Amparo à Pesquisa do Estado do Amazonas / The aim of this study was to evaluate, through microshear bond strength test (μSBS), the influence of a hemostatic solution (Aluminum Chloride 25%) to the bond strength on dentin. Thirty-six (36) bovine incisors free of any structural defects were prepared and randomly divided into 6 test groups (n = 6): SB - adhesive system Scotchbond Multi-purpose (3M/ESPE); CS – Adhesive system Clearfil SE Bond (Kuraray); SU – adhesive system Single Bond Universal (3M/ESPE); SBC, CSC and SUC groups received application of the same adhesives systems used in SB, CS and SU, respectively, with the prior application of aluminum chloride 25% hemostatic solution. Following, a nanohybrid composite Filtek Z350 XT (3M/ ESPE) was applied to the dentin surface using silicon tubes (Tygon) in order to obtain composite resin cylinders in each specimen, a total of 18 cylinders in each group. The specimens were maintained at 37 °C for 7 days and then submitted to μSBS test. The bond strength values were statistically analyzed for normality by Shapiro-Wilk test and compared using two-way ANOVA and Tukey's test at a significance level of 0.05, which showed no statistically significant differences regarding the type of adhesive and contamination. In this sense, the use of aluminum chloride 25% did not influence significantly the performances of the adhesives used on dentin in this study. / O objetivo deste estudo foi avaliar, através do teste de microcisalhamento (μSBS), a influência de uma solução hemostática (Cloreto de Alumínio 25%) na resistência de união à dentina. Trinta e seis (36) dentes incisivos bovinos isentos de quaisquer defeitos estruturais foram preparados e divididos aleatoriamente em 6 grupos (n=6) teste: SB – Sistema adesivo ScotchBond Multi-uso (3M/ESPE); CS – Sistema adesivo Clearfil SE Bond (Kuraray); SU – Sistema adesivo Single Bond Universal (3M/ESPE); os grupos SBC, CSC e SUC receberam a aplicação dos mesmos sistemas adesivos empregados nos grupos SB, CS e SU, respectivamente, no entanto, com a aplicação prévia da solução hemostática à base de Cloreto de Alumínio 25%. Na sequência, o compósito nanohíbrido Filtek Z350 XT (3M/ESPE) foi aplicado à superfície dentinária através de tubos de silicone (Tygon) de maneira a obter cilindros do compósito resinoso em cada espécime, totalizando 18 cilindros em cada grupo. Os espécimes foram mantidos em estufa (37ºC) durante 7 dias e em seguida, submetidos ao teste mecânico. Os valores de resistência de união foram analisados estatisticamente para normalidade através do teste Shapiro-Wilk e comparados usando-se ANOVA two-way e Teste de Tukey em um nível de significância de 0.05, os quais não demonstraram diferença estatística significante em relação ao tipo de adesivo e ao tipo de contaminação. Neste sentido, o uso do Cloreto de Alumínio 25% não influenciou de maneira significativa, em dentina, o desempenho dos sistemas adesivos utilizados neste estudo.

Indiana University-Purdue University Indianapolis (IUPUI) / Brasseler USA (Savannah, GA) developed and introduced a bioceramic putty called EndoSequence Root Repair Material (ERRM) that can be used as a retrofilling material for surgical endodontics. The material is said to have many of the same chemical, physical, and biological properties as mineral trioxide aggregate (MTA), but with superior handling characteristics. The material is composed of calcium silicates, monobasic calcium phosphate, zirconium oxide, tantalum oxide, proprietary fillers, and thickening agents. ERRM is said by the manufacturer to bond to adjacent dentin, have no shrinkage, be highly biocompatible, hydrophilic, radiopaque, and antibacterial due to a high pH during setting. Investigations on the sealing properties of this material have not yet been conducted. The purpose of this study was to compare the microbial leakage of Enterococcus faecalis in teeth with root-end fillings using ProRoot MTA and Brasseler’s ERRM in a dual-chamber bacterial leakage model as described by Torabinejad and colleagues. The aim of this investigation was to compare the bacterial microleakage of these two root-end filling materials exists. Sixty-two human, single-rooted, mandibular premolars in which extraction was indicated were accessed and instrumented in an orthograde fashion with hand and rotary files. Root resection of the apical 3 mm was then completed and root-end retropreparations were created for placement of root-end filling material. Twenty-seven of these premolars had root-end fillings using ProRoot MTA and 27 had root-end fillings using ERRM. Two teeth were used as a positive control group with no root-end filling, and two other teeth were used as a negative control group and were sealed and coated with dentin bonding agent. The teeth were then evaluated for microleakage using a dual-chamber bacterial microleakage model for 40 days as described by Torabinejad and colleagues. Microleakage was determined by the presence of turbidity in the lower chamber of the apparatus and was assessed each day. Fresh samples of E. faecalis were used every three days to inoculate the apparatus and serve as a bacterial challenge for the materials. Results were recorded every day for 30 days. The outcome of interest (bacterial turbidity) and time-to-leakage (in days) were determined for each of the samples. Survival analysis was used to compare the two groups with a Kaplan-Meier plot to visualize the results and a nonparametric log-rank test for the group comparison. The microleakage of ERRM was not statistically different (p > 0.05) than leakage of ProRoot MTA when subjected to E. faecalis over the 40 day observation period. Both groups had a small number of early failures (within 4 days) and no leakage was observed for the remaining 40 days of the study. Therefore, the null hypothesis was rejected. The results of this research support the use of either of these two materials when compared with the controls. The microleakage of Brasseler’s EndoSequence Root Repair Material was at least as good as ProRoot Mineral Trioxide Aggregate when tested with E. faecalis.