The sand dunes lying ten miles northwest of Kanab in Kane County, Utah, support a sparse plant cover with four species dominating the vegetation: Psoralea stenostachys, Sophora stenophylla, Oryzopsis hymenoides, and Wyethia scabra var. attenuata. Of these Psoralea and Wyethia are endemic to the dunes or to a limited area which includes the dunes. The pineer species gain a start in the valleys between dunes and occupy the area only until sand covers them or until sand is blown away from their roots. Stabilization of the soils is not permanently in the interdune valleys and the dunes continue to wander. The low fertility of the soils, the low moisture content, the extremes of temperature, light, and other environmental factors limits the number of individual plants which can occupy the dunes. The region will continue to have actively moving dunes until major climatic changes occur permittimg a denser plant cover which would tend to stabilize the sands.

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Kalaharituber pfeilii (Henn.) Trappe & Kagan-Zur commonly known as the “Kalahari truffle” is a desert truffle species identified from the Kalahari region of southern Africa. Two other species, Eremiomyces echinulatus (Trappe & Marasas) Trappe & Kagan-Zur and Mattirolomyces austroafricanus (Trappe & Marasas) Trappe & Kovacs are also known to occur in other parts of southern Africa. Truffles are hypogeous fruiting bodies of Ascomycetes, important to humans for their nutritional value and medicinal characteristics. These truffles are known as desert truffles as they prefer to occur under arid or semi-arid conditions characteristic of deserts. Truffle development depends on the presence of a mycorrhizal host, associated microorganisms as well as soil and climatic characteristics. It has been suggested that K. pfeilii has a suspected broad plant host range which includes herbaceous to woody trees and shrubs. However, these relationships have not been verified. Indigenous people of the Kalahari believe that truffles are found under grasses. In the Kalahari, truffle fruiting bodies are often found entangled in Stipagrostis ciliata (Desf.) De Winter var. capensis (Trin. & Rupr.) De Winter roots. S. ciliata, also known as the tall bushman-grass, is the most common grass found in the Kalahari. The objective of this study was to provide conclusive evidence that S. ciliata var. capensis is a host of the Kalahari truffle. Truffle fruiting bodies and grass roots from where the truffles were found were collected from Upington, South Africa. The fruiting bodies were identified by observing their morphological characteristics using the ‘Keys of Truffle genera’. All observed physical properties were similar to those of K. pfeilii and further identification was done using molecular techniques. DNA was extracted from the fruiting bodies, mycelial cultures, rhizosheaths and from the S. ciliata var. capensis grass roots, which were then amplified using the specific K. pfeilii specific primers TPF3 and TPR1 and sequenced. The obtained sequence results confirmed that the collected fruiting bodies were those of the K. pfeilii and the molecular techniques also confirmed that K. pfeilii DNA was present in the S. ciliata var. capensis rhizosheath and root cells. Microscopy showed an ectendomycorrhizal association between K. pfeilii and S. ciliata var. capensis. Mycorrhizal resynthesis experiments were conducted to establish this mycorrhizal association in-vitro. They were unsuccessful because of the structure of the grass and the availability of contaminants. And more...

The thesis focuses on ‘green’ sample preparation techniques that utilise minimal or no organic solvents thereby producing negligible volumes of organic waste, to ensure safety both to user and the environment. Volatile compounds were extracted and pre-concentrated from Kgalagadi desert truffles (kalaharituber) by headspace solid phase microextraction (HSSPME) and supercritical fluid extraction (SFE). PHWE was employed for the extraction of amino acids and fatty acids. Subsequent analysis of volatile compounds was carried out by gas chromatography coupled with mass spectrometry. Four types of HS-SPME fibers (PDMS 100 μm, PDMS 7 μm, Polyacrylate 85 μm, CAR/DVB/PDMS 50/30 μm) were evaluated. A total of 24 volatile compounds with a molecular weight range from 110 to 354, the most prominent peak being 2-t-Butyl-2,3-dimethyl-3-buten-1-ol (C₁₀H₂₀O, MW 156) were detected after sampling with a PDMS 100 μm fiber. Less volatile compounds were detected after SFE with CO2. A total of 16 amino acids were identified while 17 fatty acids (MW from 132.12 to 367.49) were also identified. The characteristic profile of the Kgalagadi desert truffle was found to contain mainly fatty acid methyl esters and unsaturated aliphatic hydrocarbons. The most prominent compound peaks identified were; 2-t-butyl-2,3-dimethyl- 3-buten-1-ol, disulfide, ethyl benzoic acid 2-4-dhydroxy-3,6-dimethyl-methyl ester, 8,11- octadecanoic acid methyl ester, benzoic acid, 2,4-dihydroxy-3,6-dimethyl-methyl ester, isoquinoline, 1 butyl-3,4-dihydro and 3-heptanone, 6 methyl. Optimization results indicated that fresh slices from the heart of truffles were the best to use for HS-SPME-GCMS volatile compound analysis as they showed a higher sensitivity

The objective of this project is to develop an activity guide for the California native plant trail located adjacent to the San Bernardino County Museum that allows families to discover native California plants while increasing environmental and cultural sensitivity.

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From the Proceedings of the 1977 Meetings of the Arizona Section - American Water Resources Assn. and the Hydrology Section - Arizona Academy of Science - April 15-16, 1977, Las Vegas, Nevada / The suitability of treated municipal sewage effluent for the irrigation of deep- rooting plant material for the rehabilitation of copper mine tailings was studied at the Cyprus Pima Mining Company. The effectiveness of treated sewage effluent was compared with well water on the growth and survival of trees, legumes and grasses. The species studied were eucalyptus (Eucalyptus rostrata), native mesquite (Prosopis juliflora), palo verde (Cercidium floridum), desert tobacco (Nicotiana lauca) barley (Hordeum vulgare), perennial rye grass (Lolium perenne), alfalfa (Medicago sativa), and blue lupine (Lupinus augustifolius). Sprinkler and tree -well irrigation methods were used to apply the treated sewage effluent and well water to steep tailing slopes. The treated municipal sewage effluent was found to be a practical irrigation substitute for well water and a good source of plant nutrients such as nitrogen and phosphorous. Effluent produced better survival and growth than did well water with or without augmentation.

From the Proceedings of the 1977 Meetings of the Arizona Section - American Water Resources Assn. and the Hydrology Section - Arizona Academy of Science - April 15-16, 1977, Las Vegas, Nevada / Soil water content was measured every two weeks during 1974-1975, using a neutron probe, at selected locations around the desert plant species creosote (Larria divaricata), bursage (Ambrosia deltoidea), and in an open space. The purpose of taking the measurements was to enable one to estimate the evapotranspiration rate of the desert plants by measuring soil moisture depletion. The sampling problem associated with measuring soil moisture, using neutron access tubes, is the number, location, and installation depth of the tubes. Analyses of the total soil moisture beneath the creosote plant showed greater variability between access tubes located near different plants the same distance from the crown of the plant than between tubes located around the same plant. Because of the size of the bursage plant, the variability in total soil moisture beneath the plant was greater among tubes around the same plant than between tubes at the same location at different plants.

From the Proceedings of the 1977 Meetings of the Arizona Section - American Water Resources Assn. and the Hydrology Section - Arizona Academy of Science - April 15-16, 1977, Las Vegas, Nevada / Transpiration rates (T) of riparian phreatophytes can be high. Antitranspirant (AT) sprays can curtail T without the ecological imbalance made by eradication. Saltcedar (Tamarix sp.) and cottonwood (Populus sp.) in 15-gal. drums enabled replicated trials on isolated plants or on canopies. T of isolate saltcedar plants could be 2x that of plants in a fairly dense canopy. T for a unit ground area of saltcedar varied from 2.2 (sparse -) to 15.8 (dense-stand) mm/day in July at Davis. Extrapolation of experimental T data to field sites must, therefore, be made carefully. Wax -based ATs increased foliar diffusive resistance (R), and reduced T of saltcedar and cottonwood 32-38% initially and 10% after 3 weeks. R increased naturally in the afternoon when evaporative demand was high and if soil water was low. Nocturnal T of salt cedar was 10% of day T. AT effectiveness increased with a higher ratio of day: night hours, and with lower soil water stress. Therefore, AT will be most effective on long summer days in riparian areas where ground water is available.