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BACKGROUND: Implant therapy has been the gold standard in the past decade when it comes to replacing partially or complete edentulous oral cavities. Patients favor this line of treatment since it does mimic their natural teeth in esthetic, function and phonetics. Unfortunately, some initially integrated implants end up diagnosed with peri-implantitis, which threatens the longevity of those implants in their respective alveolar bone. Several methods have been discussed aiming to either salvage the diseased implant or prolong the life of it in patients’ oral cavities. In this protocol we studied the efficacy of one of the suggested protocols that has been used frequently in periodontal practices aiming to decontaminate the surface of previously diseased implants. MATERIALS AND METHODS: This study looked at the efficacy through two analyses, a descriptive and a quantitative. In the descriptive, several peri-implantitis diagnosed implants were collected and distributed over 4 groups: Test, Negative Control, Positive control and compared to pristine implants. Osteoprogenitor cells were prepared in-vitro and seeded over these implants after applying the protocol on Test group only. The quantitative analysis used the EDX analysis to study the percentages of Titanium and Oxygen on contaminated implants before and after applying the protocol on. Deposits removal was tested as well to ensure efficacy of decontamination protocol. RESULTS: Descriptive analysis showed that osteoprogenitor cells had higher attachment and proliferation on implants that followed the decontamination protocol vs. other groups. Quantitative analysis showed statistically significant higher titanium percentages after decontamination. Oxygen levels were higher as well but not statistically significant. Deposits were statistically significant in removal after decontamination protocol. CONCLUSION: Decontamination of previously diseased implants following the mentioned protocol has efficiently increased the chances of re-establishment of osseointegration in previously contaminated implants.

Šis mokslinis darbas buvo atliktas Justinas Banelis Lietuvos sveikatos mokslų universitete. Vadovaujant prof. dr . Broniui Bakučiui konsultuojant lekt. dr. Violetai Baliukonienei. Šio tyrimo tema " Mikotoksinus detoksikuojančių profilaktikos priemonių pieniniams galvijams efektyvumo įvertinimas " . Puslapių skaičius 37 , 10 lentelių , 3 nuotraukos . Tyrimai buvo atlikti nuo 01/10/2011 iki 01/10/2012 LSMU VA ir gyvūnų gerovės laboratorijoje. Šio tyrimo tikslas buvo Įvertinti mikotoksinus detoksikuojančio preparato efektyvumą pieninių galvijų bandos sveikatingumui. Biocheminių , kraujo tyrimų parametrų ir pieno sudėties melžiamų karvių ir nustatyti mikotoksinu detoksikacija su komerciniu X adsorbentu. Buvo atrinktos 28 Lietuvos Žalosios veislės kliniškai sveikos vidutinio produktyvumo karvės. Karvės buvo nuo 3,43 ± 0,24 laktacijos trukmės laktacijos buvo 122 ± 15,9 dienų. Pieno išeiga karvės buvo 15,36 kg per dieną ,pieno riebalų vidurkis buvo 4,14 ± 0,25 % , baltymų kiekis 3,06 ± 0,04 % ir karbamido - 23,80 ± 1,35 mg% . Karvių pašarams buvo nustatytas mikotoksinų aflatoksino B1 ( AFL B1) , zearalenono ( ZON ), deoksinivalenolio ( DON ). Zearalenonas buvopagrindinis teršalas ir buvo rasta šiene iki 1000 mikrogramų / kg sausos medžiagos. Deoksinivalenolis buvoantras teršalų ir buvo rasta šieno lygiu iki 600 mikrogramų / kg sausos medžiagos. Didžiausias aflatoksino B1 koncentraciją - 10,0 mikrogramo / kg žemės miežių . Komercinis detoksikuojantis produktas X buvo duotas... [toliau žr. visą tekstą] / This scientific work was done by student Justinas Banelis of Lithuanian University of Health Sciences. He was supervised by prof. dr. Bronius Bakutis and consulted by lect. dr. Violeta Baliukoniene. The topic of this scientific research is “The effects of feeding mycotoxin binding products on the performance of lactating dairy cows“. Page count 37, 10 tables,3 pictures. The experiments was done from 01/10/2011 to 01/10/2012 in LUHS VA in the Animal Welfare Reaserch Laboratory. The aim of this study were to investigate the long-term exposure toxic effects of diet naturally contaminated with a low concentrations mycotoxins (AFL B1, ZON, DON) on biochemical, complete blood count parameters and milk composition of dairy cows and to determined the mycotoxins detoxification by with the commercial X adsorbent. 28 Lithuanian Red clinically healthy medium productive cows were selected. Cows were from 3.43±0.24 lactation and duration of lactation were 122±15.9 days. Milk-yield of cow was 15.36 kg per day, an average of milk fat was 4.14 ±0.25 %, protein content 3.06±0.04 % and urea – 23.80±1.35 mg %. In cow feeds were determined mycotoxins aflatoxin B1 (AFL B1), zearalenone (ZON), deoxynivalenol (DON). Zearalenone was the major contaminant and was found in the hay at level of up to 1000 µg/kg of dry matter. Deoxynivalenol was the second contaminant and was found in the hay at level of up to 600 µg/kg of dry matter. The biggest Aflatoxin B1 content - 10.0 µg/kg in ground barley. The... [to full text]

La photocatalyse est un procédé d'oxydation avancée qui consiste en l'activation du dioxyde de titane sous UV pour générer des espèces oxydantes. Ces dernières sont capables d'inactiver les cellules vivantes. Nos travaux ont porté sur l'analyse des mécanismes antimicrobiens de la photocatalyse à l'échelle cellulaire et moléculaire sur le modèle eucaryote Saccharomyces cerevisiae, champignon unicellulaire. Le traitement photocatalytique affecte de manière drastique la cultivabilité de cette levure. La diminution de la cultivabilité a été reliée à la perte de l'intégrité membranaire et à la perte de l'activité enzymatique intracellulaire, analysées par cytométrie en flux. L'exposition des levures à la photocatalyse provoque des dommages à toutes les macromolécules (acides nucléiques, lipides membranaires, protéines) et par conséquent aux structures cellulaires ce qui engendre la libération de constituants cellulaires (ions, acides aminés), de même que la formation de produits de dégradation (malondialdéhyde, acides organiques). Ces dommages peuvent être liés à un stress oxydant intracellulaire suggéré par l'accumulation des ions superoxyde dans les cellules traitées et l'augmentation de la résistance pour les souches surexprimant des enzymes de dégradation des ROS. Enfin, l'étude de l'impact de la photocatalyse sur des organismes fongiques ayant un impact environnemental ou sur la santé, a révélé l'existence de cellules ou de structures fongiques résistantes. Ces résultats ont apporté des éléments de connaissance inédits sur l'impact de la photocatalyse sur les cellules eucaryotes fongiques et ouvrent de nouvelles perspectives notamment dans la compréhension du phénomène de résistance / Photocatalysis is an advanced oxidative process that generates reactive oxygen species (ROS) and inactivates living cells. The aim of this work was to have a better understanding of the antimicrobial mechanisms generated by photocatalytic treatment. The cellular impact was monitored using the unicellular fungal model, Saccharomyces cerevisiae yeast. Photocatalysis reduces drastically the cultivability of yeast cells. Flow cytometry analyses revealed that the decrease of cell cultivability was related to both damages in plasma membrane and loss of intracellular enzymatic activity. During exposure to photocatalysis, multiple cellular macromolecules are damaged (lipids, proteins, nucleic acids). These damages are responsible for cellular structure dysfunction leading to a release of intracellular compounds (ions, amino acids) and the formation of by-products and pollutant (carboxylic acids, malondialdéhyde). The increase of intracellular superoxide ions amounts and the higher resistance of yeast strains overexpressing ROS detoxifying enzymes suggested an intracellular oxidative status responsible for described macromolecular damages. Finally, exploring photocatalytic treatment on other environmental and health impact fungi revealed the presence of resistant cells or structures. For the first time, an interdisciplinary work focusing on cellular impacts of photocatalysis was monitored leading to a better understanding and to new perspectives