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1	A microflow cytometer with simultaneous dielectrophoretic actuation for the optical assay and capacitive cytometry of individual fluid suspended bioparticles Romanuik, Sean 14 September 2009 (has links) Fluid suspended biological particles (bioparticles) flowing through a non-uniform electric field are actuated by the induced dielectrophoretic (DEP) force, known to be dependent upon the bioparticles’ dielectric phenotypes. In this work: a 10-1000 kHz DEP actuation potential applied to a co-planar microelectrode array (MEA) induces a DEP force, altering passing bioparticle trajectories as monitored using: (1) an optical assay, in which the lateral bioparticle velocities are estimated from digital video; and (2) a capacitive cytometer, in which a 1.478 GHz capacitance sensor measures the MEA capacitance perturbations induced by passing bioparticles, which is sensitive to the bioparticles’ elevations. The experimentally observed and simulated lateral velocity profiles of actuated polystyrene microspheres (PSS) and viable and heat shocked Saccharomyces cerevisiae cells verify that the bioparticles’ dielectric phenotypes can be inferred from the resultant trajectories due to the balance between the DEP force and the viscous fluid drag force. Microflow Microfluidic Cytometer Cytometry Dielectrophoretic Dielectrophoresis Interferometer Interferometric Capacitive Sensor Capacitance Sensing Capacitive Detector Capacitance Detection Polystyrene Optical Assay Electrokinetic Actuation Yeast Saccharomyces cerevisiae Single-cell Diagnostic Single-cell Diagnosis Dielectric Modeling COMSOL Tracker Trajectory Velocity Profile Capacitive Signature Capacitance Signature Microelectrode
2	A microflow cytometer with simultaneous dielectrophoretic actuation for the optical assay and capacitive cytometry of individual fluid suspended bioparticles Romanuik, Sean 14 September 2009 (has links) Fluid suspended biological particles (bioparticles) flowing through a non-uniform electric field are actuated by the induced dielectrophoretic (DEP) force, known to be dependent upon the bioparticles’ dielectric phenotypes. In this work: a 10-1000 kHz DEP actuation potential applied to a co-planar microelectrode array (MEA) induces a DEP force, altering passing bioparticle trajectories as monitored using: (1) an optical assay, in which the lateral bioparticle velocities are estimated from digital video; and (2) a capacitive cytometer, in which a 1.478 GHz capacitance sensor measures the MEA capacitance perturbations induced by passing bioparticles, which is sensitive to the bioparticles’ elevations. The experimentally observed and simulated lateral velocity profiles of actuated polystyrene microspheres (PSS) and viable and heat shocked Saccharomyces cerevisiae cells verify that the bioparticles’ dielectric phenotypes can be inferred from the resultant trajectories due to the balance between the DEP force and the viscous fluid drag force. Microflow Microfluidic Cytometer Cytometry Dielectrophoretic Dielectrophoresis Interferometer Interferometric Capacitive Sensor Capacitance Sensing Capacitive Detector Capacitance Detection Polystyrene Optical Assay Electrokinetic Actuation Yeast Saccharomyces cerevisiae Single-cell Diagnostic Single-cell Diagnosis Dielectric Modeling COMSOL Tracker Trajectory Velocity Profile Capacitive Signature Capacitance Signature Microelectrode

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A microflow cytometer with simultaneous dielectrophoretic actuation for the optical assay and capacitive cytometry of individual fluid suspended bioparticles

A microflow cytometer with simultaneous dielectrophoretic actuation for the optical assay and capacitive cytometry of individual fluid suspended bioparticles