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Cell transplantation and immunoisolation : studies on a macroencapsulation device /Rafael, Ehab, January 1900 (has links)
Diss. (sammanfattning) Stockholm : Karol. inst. / Härtill 5 uppsatser.
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Design and evaluation of chitosan and N-trimethyl chitosan chloride microspheres for intestinal drug delivery / Johannes Petrus VenterVenter, Johannes Petrus January 2005 (has links)
The absorption enhancing ability of chitosan, a linear polysaccharide, is mediated
by protonated amino groups on the C-2 position of the molecules that induce
interaction with the anionic sites on the cell membranes to subsequently alter
tight junction integrity. In neutral and basic environments, such as those found in
the small and large intestines, most chitosan molecules will lose their charge and
precipitate from solution rendering it ineffective as an absorption enhancer. To
increase the solubility of this polymer, methylation of the amino groups on the C-
2 position was proposed.
A partially quaternised and water soluble derivative of chitosan, N-trimethyl
chitosan chloride (TMC), which exhibits superior solubility in a basic environment
compared with other chitosan salts was synthesised and included in a chitosan
microbead solid drug delivery system. Two TMC derivatives were synthesised
by reductive methylation from high and medium molecular weight Chitoclear™
chitosan respectively. The degree of quaternisation calculated from the 1H-NMR
spectra for the medium molecular weight TMC (TMC-M) and the high molecular
weight TMC (TMC-H) polymers were 74.7 % and 48.5 % respectively. The mean
molecular weights of the synthesised TMC-M and TMC-H polymers were 64 100
g/mole and 233 700 g/mole respectively. The effect of different concentrations
TMC-M and TMC-H on chitosan microbeads was studied with results obtained
from scanning electron microscopy (SEM), TMC loading capacity and microbead
swelling behaviour. After selection of the most suitable TMC concentration, the
effect of varying concentration (0.1, 0.2 and 0.5 %) additives on TMC and
ibuprofen release was studied. Commonly used modified cellulose gum (Ac-di-sol®(ADS)), sodium starch glycolate (Explotab®(EXP)) and ascorbic acid (AA)
were added as disintegrants to different microbead formulations to promote
release of both the ibuprofen as model drug and TMC from the beads. It was
noticed that the loading (% drug loading capacity) of TMC-M was much lower
than that obtained with TMC-H while the inclusion of different additives in varying
concentrations did not seem to have a profound influence on the loading of either
TMC-M or TMC-H. It was further noticed from the fit factors (f1 and f2) for
dissolution profiles of eighteen chitosan microbead variations that the formulation
containing TMC-H and 0.5% (w/v) ascorbic acid was the only formulation with a
significantly higher ibuprofen and TMC-H release profile compared to all other
formulations tested.
The chitosan microbead formulation containing 2%(w/v) TMC-H and 0.5 % (w/v)
ascorbic acid (H-AA-0.5) was used for in vitro absorption studies through rat
intestine in Sweetana-Grass diffusion chambers. Chitosan containing TMC-H
(no ascorbic acid) (CHIT-H) only and a plain chitosan microbead (CHIT)
formulation was used as control formulations during the in vitro studies. Although
the H-AA-0.5 formulation exhibited the highest transport rate for ibuprofen, the
mean rate of transport (P app) obtained from the two formulations containing TMCH
(CHIT-H and H-AA-0.5) showed no significant difference in the transport rate of
ibuprofen. Compared to the CHlT formulation as control, both formulations
containing TMC-H exhibited increased ibuprofen transport across in vitro rat
jejunum. However, a statistical significant increase in transport was obtained
only from the H-AA-0.5 formulation in comparison with the CHlT formulation.
It can be concluded that the combination of high molecular weight TMC with a
low degree of quaternisation and ascorbic acid (0.5% w/v) in a chitosan
microbead lead to a statistical significant increase in the in vitro transport rate of
ibuprofen through rat jejunum. / Thesis (Ph.D. (Pharmaceutics))--North-West University, Potchefstroom Campus, 2006.
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Design and evaluation of chitosan and N-trimethyl chitosan chloride microspheres for intestinal drug delivery / Johannes Petrus VenterVenter, Johannes Petrus January 2005 (has links)
The absorption enhancing ability of chitosan, a linear polysaccharide, is mediated
by protonated amino groups on the C-2 position of the molecules that induce
interaction with the anionic sites on the cell membranes to subsequently alter
tight junction integrity. In neutral and basic environments, such as those found in
the small and large intestines, most chitosan molecules will lose their charge and
precipitate from solution rendering it ineffective as an absorption enhancer. To
increase the solubility of this polymer, methylation of the amino groups on the C-
2 position was proposed.
A partially quaternised and water soluble derivative of chitosan, N-trimethyl
chitosan chloride (TMC), which exhibits superior solubility in a basic environment
compared with other chitosan salts was synthesised and included in a chitosan
microbead solid drug delivery system. Two TMC derivatives were synthesised
by reductive methylation from high and medium molecular weight Chitoclear™
chitosan respectively. The degree of quaternisation calculated from the 1H-NMR
spectra for the medium molecular weight TMC (TMC-M) and the high molecular
weight TMC (TMC-H) polymers were 74.7 % and 48.5 % respectively. The mean
molecular weights of the synthesised TMC-M and TMC-H polymers were 64 100
g/mole and 233 700 g/mole respectively. The effect of different concentrations
TMC-M and TMC-H on chitosan microbeads was studied with results obtained
from scanning electron microscopy (SEM), TMC loading capacity and microbead
swelling behaviour. After selection of the most suitable TMC concentration, the
effect of varying concentration (0.1, 0.2 and 0.5 %) additives on TMC and
ibuprofen release was studied. Commonly used modified cellulose gum (Ac-di-sol®(ADS)), sodium starch glycolate (Explotab®(EXP)) and ascorbic acid (AA)
were added as disintegrants to different microbead formulations to promote
release of both the ibuprofen as model drug and TMC from the beads. It was
noticed that the loading (% drug loading capacity) of TMC-M was much lower
than that obtained with TMC-H while the inclusion of different additives in varying
concentrations did not seem to have a profound influence on the loading of either
TMC-M or TMC-H. It was further noticed from the fit factors (f1 and f2) for
dissolution profiles of eighteen chitosan microbead variations that the formulation
containing TMC-H and 0.5% (w/v) ascorbic acid was the only formulation with a
significantly higher ibuprofen and TMC-H release profile compared to all other
formulations tested.
The chitosan microbead formulation containing 2%(w/v) TMC-H and 0.5 % (w/v)
ascorbic acid (H-AA-0.5) was used for in vitro absorption studies through rat
intestine in Sweetana-Grass diffusion chambers. Chitosan containing TMC-H
(no ascorbic acid) (CHIT-H) only and a plain chitosan microbead (CHIT)
formulation was used as control formulations during the in vitro studies. Although
the H-AA-0.5 formulation exhibited the highest transport rate for ibuprofen, the
mean rate of transport (P app) obtained from the two formulations containing TMCH
(CHIT-H and H-AA-0.5) showed no significant difference in the transport rate of
ibuprofen. Compared to the CHlT formulation as control, both formulations
containing TMC-H exhibited increased ibuprofen transport across in vitro rat
jejunum. However, a statistical significant increase in transport was obtained
only from the H-AA-0.5 formulation in comparison with the CHlT formulation.
It can be concluded that the combination of high molecular weight TMC with a
low degree of quaternisation and ascorbic acid (0.5% w/v) in a chitosan
microbead lead to a statistical significant increase in the in vitro transport rate of
ibuprofen through rat jejunum. / Thesis (Ph.D. (Pharmaceutics))--North-West University, Potchefstroom Campus, 2006.
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Comparison of drug permeability in rat, pig and human in vitro models / Ruan JoubertJoubert, Ruan January 2015 (has links)
A crucial step in the drug discovery and development process is the assessment of membrane permeability properties of new chemical entities and researchers are constantly searching for cost-effective, high through-put models with as high as possible predictive value. In addition, a thorough understanding of the membrane permeability pathways and metabolism mechanisms are required when evaluating drug disposition and pharmacokinetics. Various in vitro methods/techniques are available to measure the rate of permeation of compounds across epithelial cell membranes to estimate oral drug absorption in humans.
The aim of this study is to compare three in vitro models (i.e. excised rat intestinal tissue, excised pig intestinal tissue and Caco-2 human cell cultures) in terms of drug permeability characteristics by means of different techniques including the Ussing type Sweetana-Grass diffusion chamber apparatus, everted sac glass apparatus and the Transwell® plate apparatus. The transport of abacavir sulphate was determined in two directions (i.e. apical-to-basolateral or AP - BL and basolateral-to-apical or BL - AP) across excised rat intestinal tissue, excised pig intestinal tissue and Caco-2 cell monolayers. The test solution was applied to the donor side and samples (200 μl) were drawn from the acceptor side at 20 min intervals for a period of 2 h. The concentration of abacavir in the samples was then measured by means of a validated high performance liquid chromatography (HPLC) method. The transepithelial electrical resistance (TEER) was measured before and after each transport experiment to give an indication of the integrity of the cell membranes. The apparent permeability coefficient (Papp) and efflux ratio (ER) values were calculated and used to compare the different methods and techniques in terms of drug permeation characteristics.
All three of the in vitro methods, in all of the techniques employed, showed higher transport of abacavir in the BL - AP direction than in the AP - BL direction. This indicates that all three in vitro methods had intact active efflux transporters over the entire study period. The excised rat intestinal method showed similar drug permeability characteristics in both techniques compared to that of the Caco-2 cell monolayers. In contrast, the excised pig intestinal method only showed similar drug permeability characteristics in the Sweetana-Grass diffusion apparatus when compared to the Caco-2 cell monolayers. This phenomenon can possibly be explained by the relatively large surface area of the pig tissue used in the everted sac technique where the role of physiological and other factors take effect. These factors may include the thickness of the membrane and mucus layer as well
as variables such as diet, age, gender and size of the pigs obtained from the abattoir that cannot be controlled. / MSc (Pharmaceutics), North-West University, Potchefstroom Campus, 2015
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Comparison of drug permeability in rat, pig and human in vitro models / Ruan JoubertJoubert, Ruan January 2015 (has links)
A crucial step in the drug discovery and development process is the assessment of membrane permeability properties of new chemical entities and researchers are constantly searching for cost-effective, high through-put models with as high as possible predictive value. In addition, a thorough understanding of the membrane permeability pathways and metabolism mechanisms are required when evaluating drug disposition and pharmacokinetics. Various in vitro methods/techniques are available to measure the rate of permeation of compounds across epithelial cell membranes to estimate oral drug absorption in humans.
The aim of this study is to compare three in vitro models (i.e. excised rat intestinal tissue, excised pig intestinal tissue and Caco-2 human cell cultures) in terms of drug permeability characteristics by means of different techniques including the Ussing type Sweetana-Grass diffusion chamber apparatus, everted sac glass apparatus and the Transwell® plate apparatus. The transport of abacavir sulphate was determined in two directions (i.e. apical-to-basolateral or AP - BL and basolateral-to-apical or BL - AP) across excised rat intestinal tissue, excised pig intestinal tissue and Caco-2 cell monolayers. The test solution was applied to the donor side and samples (200 μl) were drawn from the acceptor side at 20 min intervals for a period of 2 h. The concentration of abacavir in the samples was then measured by means of a validated high performance liquid chromatography (HPLC) method. The transepithelial electrical resistance (TEER) was measured before and after each transport experiment to give an indication of the integrity of the cell membranes. The apparent permeability coefficient (Papp) and efflux ratio (ER) values were calculated and used to compare the different methods and techniques in terms of drug permeation characteristics.
All three of the in vitro methods, in all of the techniques employed, showed higher transport of abacavir in the BL - AP direction than in the AP - BL direction. This indicates that all three in vitro methods had intact active efflux transporters over the entire study period. The excised rat intestinal method showed similar drug permeability characteristics in both techniques compared to that of the Caco-2 cell monolayers. In contrast, the excised pig intestinal method only showed similar drug permeability characteristics in the Sweetana-Grass diffusion apparatus when compared to the Caco-2 cell monolayers. This phenomenon can possibly be explained by the relatively large surface area of the pig tissue used in the everted sac technique where the role of physiological and other factors take effect. These factors may include the thickness of the membrane and mucus layer as well
as variables such as diet, age, gender and size of the pigs obtained from the abattoir that cannot be controlled. / MSc (Pharmaceutics), North-West University, Potchefstroom Campus, 2015
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Leukocyte transmigration and gene expression in healthy subjects and patients with renal failure-application of the skin chamber technique /Dadfar, Elham, January 2006 (has links)
Diss. (sammanfattning) Stockholm : Karol. inst., 2006. / Härtill 4 uppsatser.
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Aspectos do comportamento de gás metano dissolvido na água de reservatórios brasileiros / Aspects of dissolved methane behavior in water bodies of brazilian reservoirsSilva, Marcelo Bento da 07 December 2004 (has links)
Extensos corpos dágua como reservatórios hidrelétricos, aqui estudados, podem ser fontes consideráveis de gases de efeito estufa ('CH IND.4' e 'CO IND.2') devido à decomposição de parte da biomassa da floresta submersa e também pela biomassa alóctone e autóctone. Este trabalho apresenta concentrações e estimativas de fluxos difusivos e ebulitivos desses gases dissolvidos na coluna dágua, nos reservatórios hidrelétricos de Miranda (MG), Xingó (AL/SE), Ribeirão das Lages (RJ) e Represa do Lobo (SP) durante o período de outubro de 2001 a agosto de 2003. Utilizou-se, em perfis verticais, um sensor 'CH IND.4' de estado sólido comercial provido de detector de metano semicondutor, cuja superfície, ao adsorver o gás, varia sua resistência interna fornecendo sinal relacionado à concentração de gás. Foram usadas câmaras de troca difusiva na determinação de fluxos de troca de 'CH IND.4' e 'CO IND.2' na interface água-atmosfera e funis de captura de bolhas para processos ebulitivos. Mediram-se algumas variáveis físicas e químicas. Os perfis de gás metano dissolvido em água apresentaram elevadas concentrações, geralmente com valores crescentes desde próximo a superfície até a interface água-sedimento. Porém, sumidouros oxidativos presentes na coluna dágua, que consomem o gás metano no seu percurso até a superfície, juntamente com convecções impelidas pelo vento, foram responsáveis por perfis de diferentes tipos com concentrações alternadas, em conseqüência dessa ação. Fluxos difusivos também foram calculados a partir de experimentos de troca difusiva de gases dissolvidos na interface água-atmosfera resultando em emissões de 'CH IND.4' e 'CO IND.2' para atmosfera, e em alguns casos, absorção de 'CO IND.2' pelo reservatório / Extended water bodies such as the hydroelectric reservoirs here studied can be greenhouse gas sources ('CH IND.4' and 'CO IND.2') due to decay of part of submerged forest biomass and also due to allochtonous and autochtonous organic compounds. This work presents concentrations, ebullitive and diffusive fluxes of the gases dissolved in the water column in the following reservoirs : Miranda, Xingó, Ribeirão das Lages and Lobo, between October 2001 and August 2003. In the course of this work methane profiles were determined by a commercial methane sensor, a solid state device, the electrical resistance of which varies as a function of absorbed methane providing a signal related to dissolved gas concentration. Diffusion chambers were used to measure 'CH IND.4' and 'CO IND.2' exchange rates at the air-water interface; bubble capture funnels were used for ebullitive emission. Some physical and chemical variables were also measured. Dissolved methane profiles showed high concentrations, generally with increasing values from water surface to sediment. However, the oxidative methane sink in the water column consumes this gas in its upward migration path and together with wind-propelled convection produces different profile types. Diffusive flux was also calculated from diffusive exchange experiments of these gases at the air-water interface, which showed 'CH IND.4' and 'CO IND.2' emission, sometimes 'CO IND.2' absorption, by the reservoir
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Difusão transdentinária e citotoxicidade de sistemas adesivos autocondicionantesLanza, Célia Regina Moreira [UNESP] 06 July 2007 (has links) (PDF)
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lanza_crm_dr_arafo.pdf: 5945078 bytes, checksum: 092aa2094ca72bbfeb2e0b5301a1b69e (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Este estudo avaliou a difusão transdentinária e a toxicidade de sistemas adesivos autocondicionantes de diferentes valores de pH sobre células odontoblastóides MDPC-23. Sessenta discos de dentina (0,4 mm de espessura) foram obtidos de terceiros molares humanos hígidos e divididos em 6 grupos após a mensuração de sua condutância hidráulica pelo Flodec. Os discos foram montados em câmaras pulpares in vitro, onde 30.000 células foram plantadas no seu lado pulpar (área dentina exposta 0,28 cm2) e mantidas em cultura por 48 h. Após este período, os sistemas adesivos Clearfil SE Bond, Clearfil Protect Bond, Adper Prompt e Xeno III foram aplicados no lado oclusal dos discos. O sistema adesivo Single Bond foi usado como controle positivo e a solução tampão fosfato como controle negativo. A citotoxicidade foi avaliada após 24 horas pelo teste MTT e a morfologia celular por MEV. A difusão transdentinária foi qualificada por CG/EM. Os valores de MTT para os sistemas autocondicionantes, analisados pelos testes de Kruskall-Wallis e Mann-Whitney, foram estatisticamente diferentes do grupo controle negativo. Houve redução da viabilidade celular de 47,8%; 46,5%; 42,1% e 28,0% para o Clearfil SE Bond, Xeno III, Clearfil Protect Bond e Adper Prompt L-Pop, respectivamente. A redução da viabilidade celular foi inferior ao Single Bond (53,1%) para todos os sistemas. A análise por CG/MS identificou o HEMA como principal componente químico difundido pela dentina. Foi possível concluir que todos os sistemas adesivos autocondicionantes avaliados apresentaram difusão transdentinária resultando em redução do metabolismo celular, a qual não foi relacionada a sua capacidade de dissolução da smear layer e desmineralização da dentina subjancente. / This study evaluated the transdentinal diffusion and cytotoxicity of self-etching adhesive systems with different pH on the odontoblast cell line MDPC-23. Sixty dentin disks were cut from the crowns of extracted human permanent molar teeth with 0.4 mm thickness and were divided into 6 groups of similar hydraulic conductance (Flodec). The dentin disks were placed in an in vitro pulp chamber and 30.000 cells were planted on their pulpal side (area of exposed dentin 0.28 cm2). After 48 hours, the adhesive systems Clearfil SE Bond, Clearfil Protect Bond, Adper Prompt or Xeno III were applied on the occlusal side and light-cured for 10s. Single Bond was used as positive and phosphate buffer solution (PBS) as negative control group. The cytotoxicity was measured by MTT assay and cell characteristics were assessed by SEM, after 24 h. The transdentinal diffusion was qualified by GC/MS. Kruskall-Wallis and Mann-Whitney tests demonstrated a significant difference among the adhesives and PBS (p<0.05). The cellular viability reduction promoted by the self-etching systems was lower than that of Single Bond (53,1%). Cell metabolism was reduced in 47,8%; 46,5%; 42,1% and 28,0% for Clearfil SE Bond, Xeno III, Clearfil Protect Bond and Adper Prompt, respectively. HEMA was identified as the main diffused component. It was concluded that all investigated self-etching adhesives demonstrated transdentinal diffusion resulting in reduction of the cellular metabolism, which was not related to their capacity to dissolve the smear layer and demineralize the underlying dentin.
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Difusão transdentinária e citotoxicidade de sistemas adesivos autocondicionantes /Lanza, Célia Regina Moreira. January 2007 (has links)
Orientador: Josemeri Hebling / Banca: Roberval de Almeida Cruz / Banca: José Carlos Pereira / Banca: Elisa Maria Aparecida Giro / Banca: Carlos Alberto dos Santos Cruz / Resumo: Este estudo avaliou a difusão transdentinária e a toxicidade de sistemas adesivos autocondicionantes de diferentes valores de pH sobre células odontoblastóides MDPC-23. Sessenta discos de dentina (0,4 mm de espessura) foram obtidos de terceiros molares humanos hígidos e divididos em 6 grupos após a mensuração de sua condutância hidráulica pelo Flodec. Os discos foram montados em câmaras pulpares in vitro, onde 30.000 células foram plantadas no seu lado pulpar (área dentina exposta 0,28 cm2) e mantidas em cultura por 48 h. Após este período, os sistemas adesivos Clearfil SE Bond, Clearfil Protect Bond, Adper Prompt e Xeno III foram aplicados no lado oclusal dos discos. O sistema adesivo Single Bond foi usado como controle positivo e a solução tampão fosfato como controle negativo. A citotoxicidade foi avaliada após 24 horas pelo teste MTT e a morfologia celular por MEV. A difusão transdentinária foi qualificada por CG/EM. Os valores de MTT para os sistemas autocondicionantes, analisados pelos testes de Kruskall-Wallis e Mann-Whitney, foram estatisticamente diferentes do grupo controle negativo. Houve redução da viabilidade celular de 47,8%; 46,5%; 42,1% e 28,0% para o Clearfil SE Bond, Xeno III, Clearfil Protect Bond e Adper Prompt L-Pop, respectivamente. A redução da viabilidade celular foi inferior ao Single Bond (53,1%) para todos os sistemas. A análise por CG/MS identificou o HEMA como principal componente químico difundido pela dentina. Foi possível concluir que todos os sistemas adesivos autocondicionantes avaliados apresentaram difusão transdentinária resultando em redução do metabolismo celular, a qual não foi relacionada a sua capacidade de dissolução da smear layer e desmineralização da dentina subjancente. / Abstract: This study evaluated the transdentinal diffusion and cytotoxicity of self-etching adhesive systems with different pH on the odontoblast cell line MDPC-23. Sixty dentin disks were cut from the crowns of extracted human permanent molar teeth with 0.4 mm thickness and were divided into 6 groups of similar hydraulic conductance (Flodec). The dentin disks were placed in an in vitro pulp chamber and 30.000 cells were planted on their pulpal side (area of exposed dentin 0.28 cm2). After 48 hours, the adhesive systems Clearfil SE Bond, Clearfil Protect Bond, Adper Prompt or Xeno III were applied on the occlusal side and light-cured for 10s. Single Bond was used as positive and phosphate buffer solution (PBS) as negative control group. The cytotoxicity was measured by MTT assay and cell characteristics were assessed by SEM, after 24 h. The transdentinal diffusion was qualified by GC/MS. Kruskall-Wallis and Mann-Whitney tests demonstrated a significant difference among the adhesives and PBS (p<0.05). The cellular viability reduction promoted by the self-etching systems was lower than that of Single Bond (53,1%). Cell metabolism was reduced in 47,8%; 46,5%; 42,1% and 28,0% for Clearfil SE Bond, Xeno III, Clearfil Protect Bond and Adper Prompt, respectively. HEMA was identified as the main diffused component. It was concluded that all investigated self-etching adhesives demonstrated transdentinal diffusion resulting in reduction of the cellular metabolism, which was not related to their capacity to dissolve the smear layer and demineralize the underlying dentin. / Doutor
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Aspectos do comportamento de gás metano dissolvido na água de reservatórios brasileiros / Aspects of dissolved methane behavior in water bodies of brazilian reservoirsMarcelo Bento da Silva 07 December 2004 (has links)
Extensos corpos dágua como reservatórios hidrelétricos, aqui estudados, podem ser fontes consideráveis de gases de efeito estufa ('CH IND.4' e 'CO IND.2') devido à decomposição de parte da biomassa da floresta submersa e também pela biomassa alóctone e autóctone. Este trabalho apresenta concentrações e estimativas de fluxos difusivos e ebulitivos desses gases dissolvidos na coluna dágua, nos reservatórios hidrelétricos de Miranda (MG), Xingó (AL/SE), Ribeirão das Lages (RJ) e Represa do Lobo (SP) durante o período de outubro de 2001 a agosto de 2003. Utilizou-se, em perfis verticais, um sensor 'CH IND.4' de estado sólido comercial provido de detector de metano semicondutor, cuja superfície, ao adsorver o gás, varia sua resistência interna fornecendo sinal relacionado à concentração de gás. Foram usadas câmaras de troca difusiva na determinação de fluxos de troca de 'CH IND.4' e 'CO IND.2' na interface água-atmosfera e funis de captura de bolhas para processos ebulitivos. Mediram-se algumas variáveis físicas e químicas. Os perfis de gás metano dissolvido em água apresentaram elevadas concentrações, geralmente com valores crescentes desde próximo a superfície até a interface água-sedimento. Porém, sumidouros oxidativos presentes na coluna dágua, que consomem o gás metano no seu percurso até a superfície, juntamente com convecções impelidas pelo vento, foram responsáveis por perfis de diferentes tipos com concentrações alternadas, em conseqüência dessa ação. Fluxos difusivos também foram calculados a partir de experimentos de troca difusiva de gases dissolvidos na interface água-atmosfera resultando em emissões de 'CH IND.4' e 'CO IND.2' para atmosfera, e em alguns casos, absorção de 'CO IND.2' pelo reservatório / Extended water bodies such as the hydroelectric reservoirs here studied can be greenhouse gas sources ('CH IND.4' and 'CO IND.2') due to decay of part of submerged forest biomass and also due to allochtonous and autochtonous organic compounds. This work presents concentrations, ebullitive and diffusive fluxes of the gases dissolved in the water column in the following reservoirs : Miranda, Xingó, Ribeirão das Lages and Lobo, between October 2001 and August 2003. In the course of this work methane profiles were determined by a commercial methane sensor, a solid state device, the electrical resistance of which varies as a function of absorbed methane providing a signal related to dissolved gas concentration. Diffusion chambers were used to measure 'CH IND.4' and 'CO IND.2' exchange rates at the air-water interface; bubble capture funnels were used for ebullitive emission. Some physical and chemical variables were also measured. Dissolved methane profiles showed high concentrations, generally with increasing values from water surface to sediment. However, the oxidative methane sink in the water column consumes this gas in its upward migration path and together with wind-propelled convection produces different profile types. Diffusive flux was also calculated from diffusive exchange experiments of these gases at the air-water interface, which showed 'CH IND.4' and 'CO IND.2' emission, sometimes 'CO IND.2' absorption, by the reservoir
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