Made available in DSpace on 2017-07-10T14:57:33Z (GMT). No. of bitstreams: 1 Lucio_ paulo.pdf: 1888833 bytes, checksum: 7e440f531baf7b1f6f7f1d8c2cd63b02 (MD5) Previous issue date: 2016-02-19 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / The direct pulp capping aims to protect the pulp by a bioactive material, allowing the maintenance of pulp vitality and the formation of mineralized barrier. The objective of this study was histologically comparing the pulp response of different materials, for continuity and morphology of the mineralized structure. It was 108 (one hundred and eight) teeth of 54 (fifty-four) male Wistar rats, eight (8) weeks old. Circular cavities were prepared in mesio-occlusal surface of the upper first molars and capped in accordance with the Group, which included the animal: group 1 with calcium hydroxide (CH); Group 2: MTA and group 3: Biodentine®. All cavities were sealed with cement resin modified glass ionomer cement (3M Vitremer -ESPE) and the animals euthanized at predetermined periods of seven (7), fourteen (14) and 21 (twenty one) days. The jaws were removed and fixed slides prepared and stained with HE (hematoxylin and eosin) for descriptive histological evaluation of inflammation and the formation of mineralized barrier. The results showed that the MTA and the Biodentine® exhibit less intense inflammatory reactions when compared to the CH, and the statistical comparison (Kruskal-Wallis) of formation and quality of the formed dentin barrier statistically different at 21 days between the CH groups for MTA and Biodentine, which were similar to each other. It can be concluded that the MTA and Biodentine® induce mineralized architecture of a more continuous and uniform barrier and less intense when compared to pulp response to CH. / O capeamento pulpar direto visa à proteção da polpa por um material bioativo que permita a manutenção da vitalidade pulpar e a formação de barreira mineralizada. Assim, o objetivo deste estudo foi comparar histologicamente a resposta pulpar frente a materiais dentários, quanto à continuidade e morfologia da estrutura mineralizada. Foram utilizados 108 (cento e oito) dentes de 54 (cinquenta e quatro) ratos Wistar, machos com 8 (oito) semanas de idade. Cavidades circulares foram preparadas na face mesio-oclusal dos primeiros molares superiores e capeadas de acordo com o grupo ao qual pertencia o animal: grupo 1: com hidróxido de cálcio (CH); grupo 2: MTA e grupo 3: Biodentine®. Todas as cavidades foram seladas com cimento de ionômero de vidro modificado por resina (Vitremer 3M ESPE) e os animais eutanasiados em períodos predeterminados de 7 (sete), 14 (quatorze) e 21(vinte e um) dias. Posteriormente as maxilas removidas, fixadas e confeccionadas lâminas coradas H.E. (Hematoxilina e Eosina) para avaliação histológica descritiva da inflamação e da formação da barreira mineralizada. Os resultados demostraram que o MTA e o Biodentine® promovem reações inflamatórias menos intensas quando comparados ao CH. A comparação estatística (Kruskal-Wallis) da formação e da qualidade da barreira de dentina formada demonstrou diferença estatística aos 21 dias entre os grupos de CH para o MTA e Biodentine, e estes dois últimos similares entre si. Pode-se concluir que o MTA e o Biodentine® induzem a arquitetura de uma barreira mineralizada mais contínua e uniforme e a uma resposta pulpar menos intensa quando comparados ao CH.

A la suite d’une lésion carieuse ou traumatique du complexe pulpo-dentinaire, une inflammation locale est initiée. Cette inflammation peut être délétère car la pulpe dentaire est localisée au sein de parois dentinaires rigides, elle doit donc être résolue pour initier la régénération.Lors de lésions juxta-pulpaires, le praticien réalise un coiffage pulpaire direct afin de conserver la vitalité de la dent. Un biomatériau est placé au contact des tissus pulpaires afin de maintenir l’intégrité de la pulpe, en promouvant les processus de guérison tout en la protégeant du milieu buccal hostile.Les silicates de calcium sont aujourd’hui les matériaux de choix pour ces actes thérapeutiques. Des modifications de leur formulation visant à réduire leur temps de prise ont conduit au développement de silicates tricalciques photo-polymérisables. Cependant, la présence importante de résines dont la toxicité pulpaire est connue, soulève des questions. Cette thèse a pour objectif d’évaluer l’effet de différents biomatériaux de coiffage pulpaire sur l’inflammation et la régénération, au niveau cellulaire et tissulaire et d'étudier les conséquences de l'ajout de résines. Nos résultats démontrent que les matériaux de coiffage pulpaire affectent les étapes précoces de l'inflammation et de la régénération pulpaire. Alors que les silicates tricalciques modifient l'équilibre vers le processus de régénération, les biomatériaux contenant de la résine déplacent cet équilibre vers une réaction inflammatoire. Ce travail met en évidence l'importance capitale du choix du biomatériau de coiffage et restreint l'utilisation de biomatériaux contenant de la résine pour le coiffage pulpaire indirect. / Deep carious or physical lesions may lead to dental pulp injuries. Subsequently, both infiltrating bacteria and injured tissues initiate an inflammatory reaction. This may be detrimental to the healing process within the pulp inextensible environment.Clinically, during juxta-pulpal lesions, the practitioner performs a direct pulp capping in order to preserve the tooth vitality. A biomaterial is placed directly in contact with the pulp tissues to maintain pulp integrity, promoting the healing processes and protecting it from the hostile oral environment. Tricalcium silicates are now considered as the biomaterials of choice for vital pulp therapy. Modifications of this type of biomaterials aiming at reducing their setting time led to the development of light-cured tricalcium silicates. However, the presence of a high percentage of resins in this material raises questions about its possible effects on the pulp inflammatory reaction due to its reported toxicity. This aim of this thesis was to determine the influence of tricalcium silicate-based capping biomaterials on the initial steps of pulp inflammation and healing and to investigate the consequences of adding resins.Our results demonstrate that pulp capping biomaterials affect the early steps of pulp inflammation and healing. While tricalcium silicates shift the balance towards the healing process, resin-containing biomaterials drive this balance towards an inflammatory reaction. Overall, this work highlights the fact that the choice of pulp capping biomaterial is of prime importance in direct pulp capping procedures and does not support using resin containing biomaterials for direct pulp capping.