Typescript. / Thesis (Ph. D.)--University of Hawaii at Manoa, 1975. / Bibliography: leaves [130]-141. / xi, 141 leaves ill. (some col.)

Typescript. Thesis (Ph. D.)--University of Hawaii, 1934. Bibliography: leaves 122-124.

Thesis (M.S.)--University of Hawaii at Manoa, 2005. / Includes bibliographical references (leaves 66-68). / x, 68 leaves, bound col. ill. 29 cm

Biological, serological, and molecular characteristics of the P4 pathotype of pea seedborne mosaic potyvirus (PSbMV) were investigated. The characterization allowed P4 to be differentiated from other pathotypes of the same virus. P4 was distinguished from P1 on the basis of host symptomatology. It induced varied, but often distinctive symptoms in systemically-infected pea (Pisum sativum) cultivars. P4 inoculation of indicator hosts, notably Chenopodium album, resulted in the formation of local lesions. Pathotype P4 was seed transmitted at low frequencies relative to P1. In ten selected pea cultivars, seed transmission of P4 never exceeded 0.7%, while P1 was transmitted at frequencies as high as 33%. In mixed infections with P1, pathotype P4 was seed transmitted at 0- 2% as determined by P4-specific indirect ELISA. However, limited polymerase chain reaction (PCR) data suggest that P4 may be transmitted with P1 at higher rates than indicated by ELISA data. P4 was also aphid transmitted at relatively low rates. Pea aphids (Acyrthosiphon pisum) allowed 3 or 5 min acquisition access periods (AAP's) transmitted P4 at a maximum rate of 16% (3 aphids/plant). P4 transmission required short (1-7 min) AAP's and exhibited no bimodal character within tested parameters. P4 could be serologically distinguished from the other pathotypes using monoclonal and polyclonal antibodies. Tests with cross-reactive P4 antiserum suggest that P4 is more closely related to P2 than P1. P4-specific antiserum was derived by cross-absorbing diluted antiserum with P1- infected pea sap to eliminate P1- reactive antibodies. P1 and P4 RNA was detected and differentiated using pathotype-specific oligonucleotide primers in conjunction with PCR. Viral RNA sequences were amplified in pure preparations as well as crude pea tissue (leaf, root, pollen, and seed) extracts. / Graduation date: 1993

Copies of author's previously published articles inserted. / Bibliography: leaves 128-143. / xi, 143, [44] leaves, [36] leaves of plates : ill. (some col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / This thesis describes the development of serological and nucleic acid based diagnostic methods for pea-seed borne mosaic virus (PSbMV), the isolation of specific effects on infected pea plants, the collection and biological comparison of new PSbMV isolates from Pakistan, the cloning and sequencing of specific parts of the genome of selected isolates, nucleotide and amino acid sequence comparisons between selected isolates, and the development of a ribonuclease protection assay (RPA) for identifying genomic differences among the PSbMV isolates. It is the first comparison of a range of geographically different isolates of PSbMV on the basis of both biological and molecular properties. / Thesis (Ph.D.)--University of Adelaide, Dept. of Crop Protection, 1999

Bibliography: leaves 170-189. / xi, 189 leaves : ill. (some col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / This thesis looks at aphid feeding determinants by type and location. It examines the role of leaf surface chemicals in the discrimination of host plants and the deterrent effect of catechin and its oxidative condensation products. / Thesis (Ph.D.)--University of Adelaide, Dept. of Crop Protection, 1992

viii, 132 leaves : ill. photos, tables ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Entomology, 1978

x, 145 leaves : ill., tables, map graphs., photos ; 31 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.1980)--University of Adelaide, Dept. of Entomology, 1980

xi, 157 leaves : ill., graphs, tables ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Entomology, 1980

xiv, 253 leaves : ill. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.) from the Dept. of Entomology, University of Adelaide, 1971