Thesis (M.S.)--Oregon State University, 1978. / Typescript (photocopy). Includes bibliographical references. Also available on the World Wide Web.

Includes bibliographical references (leaves 263-268). / vii, 268 leaves : ill. (some col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Two heterocyclic chemistry projects were investigated to establish whether new methods for synthesis of substituted dendralene precursors and green tea catechins were viable. / Thesis (Ph.D.)--Adelaide University, Dept. of Chemistry, 2001

Thesis (Ph. D.)--University of Adelaide, Dept. of Crop Protection, 1992. / Includes bibliographical references (leaves 170-189).

Thesis (M. Phil.)--University of Hong Kong, 2008. / Includes bibliographical references (leaf 131-166) Also available in print.

Thesis (M. Med. Sc.)--University of Hong Kong, 1999. / Includes bibliographical references (leaves 32-36).

Thesis (Ph. D.)--University of Hong Kong, 2004. / Also available in print.

Thesis (M.Med.Sc.)--University of Hong Kong, 1999. / Includes bibliographical references (leaves 32-36). Also available in print.

Thesis (M. Med. Sc.)--University of Hong Kong, 2006.

by Tung Kai Chiu. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1999. / Includes bibliographical references (leaves 152-166). / Abstracts in English and Chinese. / STATEMENT --- p.i / ACKNOWLEDGEMENTS --- p.ii / ABBREVIATIONS --- p.iv / ABSTRACT --- p.vii / 撮要 --- p.x / TABLE OF CONTENTS --- p.xiii / Chapter CHAPTER 1: --- GENERAL INTRODUCTION / Chapter 1.1 --- AN OVERVIEW OF THE IMMUNE SYSTEM --- p.1 / Chapter 1.1.1 --- Innate Immunity --- p.1 / Chapter 1.1.2 --- Specific Acquired Immunity --- p.4 / Chapter 1.2 --- IMMUNE RESPONSE TO TUMOURS --- p.7 / Chapter 1.2.1 --- Specific Mechanisms --- p.7 / Chapter 1.2.1.1 --- Cellular Immune Response --- p.7 / Chapter 1.2.1.2 --- Humoral Immune Response --- p.9 / Chapter 1.2.2 --- Non-specific Mechanism --- p.10 / Chapter 1.2.2.1 --- Natural Killer Cells --- p.10 / Chapter 1.2.2.2 --- Lymphokine-activated Cells --- p.11 / Chapter 1.2.2.3 --- Tumour Infiltrating Lymphocytes --- p.12 / Chapter 1.2.2.4 --- Macrophages --- p.13 / Chapter 1.2.2.5 --- Other Cell Types Mediating Non-specific Immunity --- p.14 / Chapter 1.3 --- PHYTOCHEMICALS AS POTENTIAL IMMUNOMODULATORS and anti-tumour agents --- p.15 / Chapter 1.3.1 --- Modulation of the Immune System --- p.15 / Chapter 1.3.2 --- Induction of Cancer Cell Differentiation --- p.16 / Chapter 1.3.3 --- Stimulation of Apoptosis --- p.17 / Chapter 1.3.4 --- Other Anti-tumour Mechanisms --- p.18 / Chapter 1.4 --- GREEN TEA: GENERAL PROPERTIES AND PHARMACO- logical activities --- p.19 / Chapter 1.4.1 --- General Introduction --- p.19 / Chapter 1.4.2 --- Chemistry of Tea --- p.21 / Chapter 1.4.3 --- Physiological and Pharmacological Effects of Green Tea Catechins --- p.26 / Chapter 1.4.3.1 --- Anti-oxidative Activity --- p.26 / Chapter 1.4.3.2 --- Anti-mutagenic Activity --- p.27 / Chapter 1.4.3.3 --- Anti-carcinogenic Activity --- p.27 / Chapter 1.4.3.4 --- Anti-inflammatory Activity --- p.32 / Chapter 1.4.3.5 --- Hypocholesterolemic and Hypolipidemic Activities --- p.32 / Chapter 1.4.3.6 --- Anti-microbial Activity --- p.33 / Chapter 1.5 --- aims and scopes of this investigation --- p.34 / Chapter CHAPTER 2: --- MATERIALS AND METHODS / Chapter 2.1 --- MATERIALS --- p.36 / Chapter 2.1.1 --- Animals --- p.36 / Chapter 2.1.2 --- Cell lines --- p.36 / Chapter 2.1.3 --- Green Tea Catechins and Epicatechin Isomers --- p.38 / Chapter 2.1.4 --- Recombinant Murine Interleukin-2 (rmIL-2) --- p.38 / Chapter 2.1.5 --- Antibodies --- p.39 / Chapter 2.1.6 --- "Buffers, Culture Medium and Other Reagents" --- p.40 / Chapter 2.1.7 --- Reagents for Gel Electrophoresis --- p.47 / Chapter 2.1.8 --- Radioisotopes --- p.48 / Chapter 2.2 --- METHODS --- p.49 / Chapter 2.2.1 --- "Isolation, Purification and Characterization of Green Tea Catechins and Epicatechin Isomers" --- p.49 / Chapter 2.2.1.1 --- Extraction of Green Tea Catechins --- p.49 / Chapter 2.2.1.2 --- Analysis of Epicatechin Isomers by HPLC --- p.49 / Chapter 2.2.1.3 --- Determination of the Bio-toxicity of Green Tea Catechins --- p.50 / Chapter 2.2.1.4 --- Assay of In Vitro Cytotoxicity of Green Tea Catechins on Splenocytes --- p.51 / Chapter 2.2.2 --- Assays for the Immunomodulatory Activities of Green Tea Catechins --- p.52 / Chapter 2.2.2.1 --- Isolation and Preparation of Cells --- p.52 / Chapter 2.2.2.2 --- In Vitro Lymphocyte Transformation Assay --- p.53 / Chapter 2.2.2.3 --- Mixed Lymphocyte Culture --- p.53 / Chapter 2.2.2.4 --- Colorimetric Assay of Alloreactive Cytotoxic T Lymphocytes --- p.54 / Chapter 2.2.2.5 --- Foodpad Swelling Assay of Delayed-type Hypersensitivity --- p.55 / Chapter 2.2.2.6 --- Haemagglutination Assay of In Vivo Antibody Formation --- p.56 / Chapter 2.2.2.7 --- Characterization of the Lymphocyte Subpopulations from Spleen by Flow Cytometry --- p.56 / Chapter 2.2.2.8 --- In Vivo Migration of Macrophages --- p.57 / Chapter 2.2.2.9 --- In Vitro Assay of Phagocytic Activity of PEC --- p.58 / Chapter 2.2.2.10 --- In Vitro Assay of Macrophage-mediated Cytostatic Activity --- p.58 / Chapter 2.2.3 --- Assays for the Anti-tumour Activities of Green Tea Catechins --- p.60 / Chapter 2.2.3.1 --- Assay of In Vivo Anti-tumour Activity --- p.60 / Chapter 2.2.3.2 --- Induction and Assay of Natural Killer Cell Activity --- p.60 / Chapter 2.2.3.3 --- Induction and Assay of Lymphokine-activated Killer Cell Activity --- p.61 / Chapter 2.2.3.4 --- Assay of In Vitro Tumour Cell Proliferation --- p.62 / Chapter 2.2.3.5 --- In Vitro Tumour Clonogenicity Assay --- p.63 / Chapter 2.2.3.6 --- Induction of Myeloid Leukemic Cell Differentiation --- p.63 / Chapter 2.2.3.7 --- DNA Fragmentation Analysis --- p.64 / Chapter 2.2.3.8 --- Cell Cycle and DNA Content Evaluation --- p.66 / Chapter 2.2.4 --- Statistical Analysis --- p.66 / Chapter CHAPTER 3: --- "Extraction, Purification and Characterization of GTCs" / Chapter 3.1 --- INTRODUCTION --- p.67 / Chapter 3.2 --- RESULTS --- p.69 / Chapter 3.2.1 --- "Extraction of Catechins from the Chinese Green Tea, Ji Pin Long Jing" --- p.69 / Chapter 3.2.2 --- Analysis of Epicatechin Isomers by HPLC --- p.69 / Chapter 3.2.3 --- Bio-toxicity Determination by Brine Shrimp Bioassay --- p.71 / Chapter 3.2.4 --- Effect of GTCs on the Viability of Murine Splenocytes --- p.72 / Chapter 3.3 --- DISCUSSION --- p.74 / Chapter CHAPTER 4: --- The Immunomodulatory Activities of GTCs / Chapter 4.1 --- INTRODUCTION --- p.75 / Chapter 4.2 --- RESULTS --- p.77 / Chapter 4.2.1 --- Effects of GTCs on the In Vitro Proliferation of Murine Splenocytes --- p.77 / Chapter 4.2.2 --- In Vitro Co-mitogenic Effect of GTCs on Murine Splenocytes --- p.77 / Chapter 4.2.3 --- Stimulation of Lymphocyte Proliferation In Vitro by Intraperitoneal Administration of GTCs --- p.81 / Chapter 4.2.4 --- Effect of In Vivo Treatment of GTCs on In Vitro Mixed Lymphocyte Reaction --- p.81 / Chapter 4.2.5 --- Effect of In Vivo Administration of GTCs on Splenic Lymphocytes Subpopulations --- p.87 / Chapter 4.2.6 --- Effect of In Vivo Administration of GTCs on the Induction of Alloreactive Cytotoxic T Lymphocytes --- p.87 / Chapter 4.2.7 --- In Vivo Induction of Delayed-type Hypersensitivity (DTH) Response to Sheep Red Blood Cells (SRBC) by GTCs --- p.91 / Chapter 4.2.8 --- Primary Humoral Immune Response to SRBC in GTCs- treated Mice --- p.91 / Chapter 4.2.9 --- Effect of GTCs on In Vivo Migration of Macrophages --- p.95 / Chapter 4.2.10 --- Effect of GTCs on the Phagocytic Activity of Thioglycollate- elicited Macrophages In Vitro --- p.95 / Chapter 4.2.11 --- Effect of In Vivo Administration of GTCs on Phagocytic Activity of Thioglycollate-elicited Macrophages In Vitro --- p.98 / Chapter 4.2.12 --- Effect of GTCs on In Vitro Cytostatic Activity of Picolinic Acid-activated Macrophages --- p.98 / Chapter 4.2.13 --- Effect of In Vivo Administration of GTCs on the Cytostatic Activity of Picolinic Acid-activated Macrophages --- p.101 / Chapter 4.3 --- DISCUSSION --- p.103 / Chapter CHAPTER 5: --- THE ANTI-TUMOUR ACTIVITIES OF GTCs / Chapter 5.1 --- introduction --- p.107 / Chapter 5.2 --- RESULTS --- p.109 / Chapter 5.2.1 --- Effects of GTCs on the Growth of Transplantable Tumour Cells In Vivo --- p.109 / Chapter 5.2.2 --- Effect of GTCs on In Vivo Induction of Natural Killer Cells --- p.109 / Chapter 5.2.3 --- Effect of GTCs on In Vitro Induction of Lymphokine- activated Killer Cell Activity --- p.113 / Chapter 5.2.4 --- In Vitro Cytotoxicity of Green Tea Epicatechin Isomers on Murine and Human Tumour Cell Lines --- p.115 / Chapter 5.2.5 --- In Vitro Cytostatic Effect of EGCG on Various Tumour Cell Lines --- p.118 / Chapter 5.2.6 --- Effect of EGCG on the In Vitro Clonogenicity of Myeloid Leukemia Cells --- p.127 / Chapter 5.2.7 --- Induction of Apoptosis of Myeloid Leukemia HL-60 Cells In Vitro --- p.130 / Chapter 5.2.8 --- Effect of EGCG on the Cell Cycle Kinetics of Myeloid Leukemia HL-60 Cells In Vitro --- p.131 / Chapter 5.2.9 --- Effect of EGCG on the Morphological Changes of Myeloid Leukemia Cells --- p.136 / Chapter 5.2.10 --- Effect of EGCG on the Endocytic Activity of Myeloid Leukemia Cells --- p.136 / Chapter 5.3 --- discussion --- p.141 / Chapter CHAPTER 6: --- CONCLUSIONS AND FUTURE PERSPEC- TIVES --- p.145 / REFERENCES --- p.152

Chen Yu. / Thesis submitted in: November 2001. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2001. / Includes bibliographical references (leaves 98-112). / Abstracts in English and Chinese. / Acknowledgements --- p.I / List of publications --- p.II / Abstract --- p.III / Abstract (Chinese) --- p.IV / Abbreviations --- p.V / Chapter Chapter 1 --- Introduction --- p.1 / Chapter 1.1 --- Tea --- p.1 / Chapter 1.2 --- Green tea --- p.3 / Chapter a) --- Chemical composition of green tea --- p.3 / Chapter b) --- Pharmacological activities of green tea polyphenols --- p.6 / Chapter c) --- Pharmacokinetics of green tea polyphenols --- p.10 / Chapter 1.3 --- Objective --- p.14 / Chapter Chapter 2 --- Validation of analysis method for tea catechins --- p.15 / Chapter 2.1 --- Materials and methods --- p.16 / Chapter a) --- Preparation of a catechin-mixture from tea --- p.16 / Chapter b) --- Preparation of stock solutions --- p.18 / Chapter c) --- Preparation of biofluid samples --- p.18 / Chapter d) --- HPLC analysis of tea catechins --- p.19 / Chapter 2.2 --- Results --- p.21 / Chapter a) --- Catechin-mixture (tea extracts) --- p.21 / Chapter b) --- "Extraction from plasma, urine and feces" --- p.21 / Chapter c) --- HPLC analysis of biofluid samples --- p.23 / Chapter 2.4 --- Discussion --- p.26 / Chapter Chapter 3 --- Pharmacokinetics of tea catechins following administration of different doses of the catechin-mixture --- p.32 / Chapter 3.1 --- Materials and methods --- p.33 / Chapter a) --- Surgery and animal maintenance --- p.33 / Chapter b) --- Dosing and sample collection --- p.33 / Chapter c) --- Pharmacokinetics analysis of tea catechins --- p.35 / Chapter 3.2 --- Results --- p.36 / Chapter 3.3 --- Discussion --- p.50 / Chapter Chapter 4 --- Pharmacokinetics of tea catechins following administration of different doses of individual catechins --- p.52 / Chapter 4.1 --- Materials and methods --- p.53 / Chapter a) --- Chemicals and reagents --- p.53 / Chapter b) --- Pharmacokinetic study of tea catechins and the HPLC analysis --- p.53 / Chapter c) --- Pharmacokinetic analysis of tea catechins --- p.54 / Chapter 4.2 --- Results --- p.55 / Chapter 4.3 --- Discussion --- p.67 / Chapter Chapter 5 --- Plasma protein binding of tea catechins --- p.69 / Chapter 5.1 --- Introduction --- p.69 / Chapter 5.2 --- Materials and methods --- p.72 / Chapter a) --- Ultrafiltration --- p.72 / Chapter b) --- Preparation of stock solution --- p.73 / Chapter c) --- Determination of nonspecific binding of the catechins --- p.73 / Chapter d) --- Determination of ultrafiltration conditions --- p.74 / Chapter e) --- In vitro plasma protein binding assay --- p.74 / Chapter f) --- Statistical analysis --- p.75 / Chapter 5.3 --- Results --- p.76 / Chapter a) --- Nonspecific binding in ultrafiltration --- p.76 / Chapter b) --- Protein binding of the catechins --- p.76 / Chapter c) --- Statistical analysis --- p.76 / Chapter 5.4 --- Discussion --- p.80 / Chapter Chapter 6 --- Partition of tea catechins in red blood cell --- p.82 / Chapter 6.1 --- Materials and methods --- p.83 / Chapter a) --- Prepare of stock solution --- p.83 / Chapter b) --- In-vitro erythrocyte partition --- p.83 / Chapter c) --- Data Analysis --- p.83 / Chapter 6.2 --- Results --- p.85 / Chapter a) --- Selection of experiment conditions --- p.85 / Chapter b) --- Partition of catechins to RBC --- p.85 / Chapter 6.3 --- Discussion --- p.87 / Chapter Chapter 7 --- Comparison of pharmacokinetics of tea catechins in mixture form versus pure compound --- p.89 / Chapter Chapter 8 --- Conclusion --- p.95 / References --- p.98