Master of Science / Department of Grain Science and Industry / Yong Cheng Shi / Isolation of hemicellulose from distillers dry grain with solubles (DDGS) was investigated. Hemicellulose fiber gum (HFG) is a mixture of hemicelluose, protein, ash and starch. It was extracted from a commercial DDGS by heating with or without alkali. Three extraction methods (water heating, alkaline heating and alkaline hydrogen peroxide heating) were evaluated. Yield of HFG and the recovery of hemicellulose were obtained. High heating temperature (100 and 120°C), alkali or hydrogen peroxide facilitated the release of hemicellulose from the cell wall matrix. However combining alkali with 2.5% H2O2 did not extract more hemicellulose out than did alkali alone. The highest hemicellulose recovery was 32% achieved by cooking at 120°C with 2% alkaline solution. Hemicellulose can function as an emulsifier in the oil-in-water emulsions, such as beverage, and potentially replace gum arabic. HFGs obtained by a series of extracting methods were applied in both the concentrated emulsion with the gum: oil: water ratio of 0.5:1: 8.5 and the diluted emulsion with the gum: oil: water ratio of 0.005: 0.01: 1. The emulsion stability was evaluated by turbidity and creaming test. HFG extracted by 2% NaOH solution at 120°C and HFG extracted by 2% NaOH and 2.5% H2O2 solution at 100°C showed the best emulsifying ability among 15 HFG samples. DDGS was produced from corn, sorghum, wheat in the lab. HFGs extracted from sorghum and wheat DDGS were compared with that from corn DDGS. The composition of the three DDGS varied in protein, fat and non-starch carbohydrate contents. Sorghum and wheat DDGS contained higher levels of protein and lower levels of fat and non-starch carbohydrate than corn DDGS. HFG was extracted by 2% NaOH solution at 100°C for one hour and purified by 100% ethanol. The yield of HFG from corn, sorghum and wheat DDGS was 21.08, 11.07, 11.64% respectively, while the hemicellulose recovery was 30.95, 29.74, 22.71% respectively. The water extractable hemicelluloses from all three DDGS had similar ratios of arabinose to xylose.

This study assessed the value of triticale dried distillers grains with solubles(DDGS) in a feedlot finishing diet using 144 intact, and 16 ruminally cannulated crossbred yearling steers. Substituting triticale DDGS for a portion of dry-rolled barley grain (20% diet DM) decreased the prevalence of ruminal acidosis and tended to increase dry matter intake and fat deposition, but increased the incidence and severity of liver abscesses. Further substitution of triticale DDGS for barley silage (5 and 10% diet DM) increased the prevalence of ruminal acidosis, but tended to improve feed efficiency without affecting carcass characteristics. These findings suggest that feedlot finishing diets containing triticale DDGS allow producers to decrease dietary forage inclusion without affecting performance, but may require use of an antimicrobial to control liver abscesses. / Animal Science

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Master of Science / Department of Animal Sciences and Industry / Joel M. DeRouchey / A total of 6,858 pigs were used in 6 experiments to evaluate the effects of crude glycerol, dried distillers grains with solubles (DDGS), Ractopamine HCl (RAC), NutriDense corn, and feeder adjustment on growing and finishing pig performance. In Exp. 1, pigs were fed diets with 0, 2.5, or 5% crude glycerol with 0 or 20% DDGS. Adding DDGS increased ADFI and decreased G:F with no differences for pigs fed glycerol. Neither glycerol nor DDGS affected any carcass characteristics. Pigs fed DDGS had increased iodine value in carcass fat. In Exp. 2, pigs were fed diets with 0 or 5% glycerol with 0 or 7.5 ppm RAC. Feeding RAC increased ADG and G:F and decreased ADFI while glycerol tended to improve G:F. Ractopamine HCl improved carcass traits. Loin chop drip loss worsened when glycerol and RAC were added separately, however, drip loss decreased when the combination of both were fed. Glycerol did not affect loin characteristics. Neither RAC nor glycerol influenced iodine value of carcass fat. Exp. 3 and 4 were conducted to determine the 4th limiting amino acid in diets containing NutriDense corn. In Exp. 3, pigs fed the positive control and the diet with added Ile, Trp, and Val (in combination) had greater ADG. Pigs fed added Ile or Trp had greater ADG than pigs fed the negative control indicates these amino acids were co-4th limiting for 37 to 59 kg pigs. In Exp. 4, pigs fed the positive control, added Trp, or the combination of added Ile, Trp and Val had greater ADG then pigs fed the negative control or pigs fed either Ile or Val indicates these amino acids were co-4th limiting for 77 to 100 kg pigs. Exp. 5 and 6 evaluated feeder adjustment on growth performance of finishing pigs. In Exp. 5, reducing feeder opening decreased ADFI. In Exp. 6, pigs were fed at three feeder opening and either a corn-soybean meal or byproduct-based diet. Diet type did not affect pig performance. Widening feeder openings increased ADG and ADFI. Feeder setting tended to influence G:F with the best G:F at the intermediate opening.

Master of Science / Animal Sciences and Industry / John R. Jaeger / Cattle consuming low-protein forage (<7% CP) require additional supplemental protein to maintain BW and BCS. Daily delivery of protein supplements places undue financial burden on cattle producers. Supplementing cows as infrequently as once every 6 d) has resulted in similar changes cow BW and BCS when compared to daily supplementation. As calving season nears, producers may wish to increase supplementation frequency. The responses to a change in supplementation frequency during the third trimester of gestation have not been widely investigated. Therefore, our objective in Study 1 was to evaluate the effect of altering supplementation frequency during late gestation on performance of spring-calving cows grazing low-quality, dormant native range and supplemented with dried distillers grains with solubles (DDG). Angus × cows (n = 238; mean age = 6 ± 2.5 yr; average initial BW = 618 ± 56.2 kg; average initial BCS = 5.7 ± 0.03) were stratified by age, BW, BCS, and assigned randomly to 1 of 4 treatments: 1) DDG daily (D1); 2) DDG once every 6 d (D6); 3) DDG daily from d 1 to d 60 and then every 6 d (D1-D6); 4) DDG every 6 d from d 1 to d 60 and then daily (D6-D1). Treatments were initiated 100 d prior to expected onset of calving. Cow BW and BCS were measured every 28 d. Cows were sorted daily before supplementation at 0830 h. Supplement delivery was calculated to meet dietary CP requirements. Increasing supplementation frequency 28 d prepartum negatively affected final BW and BW change from d 61-88 for the D6-D1 supplementation group (P < 0.05) compared to other supplementation groups. Cow BW change for the study (d 1-88) was also less (P < 0.02) for the D6-D1 group compared to other groups but was also affected (P < 0.01) by year. Under the conditions of our study, increasing supplementation frequency 28 d before calving was not a viable means of increasing prepartum cow performance. The development of replacement heifers is a significant expense for cow-calf producers. Reducing the cost of heifer development programs while achieving high pregnancy rates is an industry-wide goal. Therefore, our objective in Study 2 was to determine if DDGS was a viable replacement for an oilseed meal-based protein supplement when developing heifers on low-quality, dormant native range. Treatments consisted of daily supplementation of either 1.65 kg DM DDG (DDG; 0.57 kg CP) or 1.37 kg DM of a 73.6% soybean meal and 26.4% rolled sorghum grain mixture (SBM-S; 0.56 kg CP). Treatments were administered from 1/15 until 4/8 (84 d). Initial BW and BCS were not different between treatments (P ≥ 0.29). Final BW and BCS also did not differ (P ≥ 0.55) between treatments; moreover, rates of BW and BCS change were not different (P ˃ 0.30) between treatments. Proportions of heifers pubertal before ovulation synchronization, first service conception rates, and final pregnancy rates were not affected (P > 0.40) by treatment. Under the conditions of our study supplemental CP fed at a rate of approximately 0.56 kg daily was sufficient to promote growth and BCS change adequate for optimal reproductive performance; moreover, supplement ruminal degradability of CP did not influence heifer performance over an 84-d development period.

This study was conducted to determine if nutrient utilization and enteric methane (CH4) emissions could be improved in overwintering beef cows consuming low-quality forage supplemented with protein in the form of dried distillers grain with solubles (DDGS) in thermal-neutral and cold-stressed environments. Thirty mature, dry and non-pregnant beef cows were divided into three treatment groups and fed diets consisting of low-quality (6.0% crude protein; CP) forage with no DDGS (control, CON), 10% DDGS (borderline sufficient CP, 8.7% CP), or 20% DDGS (excess CP, 11.6% CP). Cold acclimatization did not appear to affect nutrient intake and digestibility by beef cows, but increased N and P excretion by 1.2x and 2.5x, respectively. Cold acclimatized cows reduced energy excretion by 26.8% (7.1 vs. 5.2 ± 0.30% GEI in fall and winter, respectively; P < 0.0001) in accordance with a 33.8% increase in rumen fluid rate of passage (ROP). Supplementation with DDGS improved digestibility of N and P (40.6 vs. 61.2 ± 2.45% N and -23.9 vs. 5.7 ± 5.95% P for CON and 20%DDGS, respectively; P < 0.0001) by increasing digestible substrate in the diet. Protein supplementation increased rumen NH3-N concentrations (1.5, 2.1 and 3.1 ± 0.15 mg 100 mL-1; P < 0.0001) enough to increase rumen fermentation efficiency, resulting in 18.5% lower enteric CH4 emissions when CP was fed in excess of animal requirements. Total excretion of N and P were increased two- and 45-fold, respectively, when excess CP was fed. Reduced enteric CH4 emissions as a result of cold acclimatization suggest an advantage for the Canadian beef herd in terms of environmental sustainability. Supplementing CP in excess of cow requirements may improve nutrient utilization and rumen fermentation efficiency, and mitigate enteric CH4 emissions in beef cows fed low-quality forage diets, but may also contribute to greater N and P loading of soil and ground water.

This study was conducted to determine if nutrient utilization and enteric methane (CH4) emissions could be improved in overwintering beef cows consuming low-quality forage supplemented with protein in the form of dried distillers grain with solubles (DDGS) in thermal-neutral and cold-stressed environments. Thirty mature, dry and non-pregnant beef cows were divided into three treatment groups and fed diets consisting of low-quality (6.0% crude protein; CP) forage with no DDGS (control, CON), 10% DDGS (borderline sufficient CP, 8.7% CP), or 20% DDGS (excess CP, 11.6% CP). Cold acclimatization did not appear to affect nutrient intake and digestibility by beef cows, but increased N and P excretion by 1.2x and 2.5x, respectively. Cold acclimatized cows reduced energy excretion by 26.8% (7.1 vs. 5.2 ± 0.30% GEI in fall and winter, respectively; P < 0.0001) in accordance with a 33.8% increase in rumen fluid rate of passage (ROP). Supplementation with DDGS improved digestibility of N and P (40.6 vs. 61.2 ± 2.45% N and -23.9 vs. 5.7 ± 5.95% P for CON and 20%DDGS, respectively; P < 0.0001) by increasing digestible substrate in the diet. Protein supplementation increased rumen NH3-N concentrations (1.5, 2.1 and 3.1 ± 0.15 mg 100 mL-1; P < 0.0001) enough to increase rumen fermentation efficiency, resulting in 18.5% lower enteric CH4 emissions when CP was fed in excess of animal requirements. Total excretion of N and P were increased two- and 45-fold, respectively, when excess CP was fed. Reduced enteric CH4 emissions as a result of cold acclimatization suggest an advantage for the Canadian beef herd in terms of environmental sustainability. Supplementing CP in excess of cow requirements may improve nutrient utilization and rumen fermentation efficiency, and mitigate enteric CH4 emissions in beef cows fed low-quality forage diets, but may also contribute to greater N and P loading of soil and ground water.