A trial was conducted to evaluate the accuracy of the 1996 NCR beef model to predict DMI and ADG of pregnant cows under western Canadian conditions. Over two consecutive years, 90 Angus (587±147 kg) cows assigned to 15 pens (N=6) were fed typical diets ad libitum, formulated to stage of pregnancy. Data collection included pen DMI and ADG (corrected for pregnancy), calving date, calf weight, body condition scores and ultrasound fat measurements, weekly feed samples and daily ambient temperature. DMI and ADG for each pen of cows in each trimester was predicted using the computer program Cowbytes based on the 1996 NRC beef model. The results indicate that in the 2nd and 3rd trimester of both years the model under predicted (P≤0.05) ADG based on observed DMI. Ad libitum intake was over predicted (P≤0.05) during the 2nd trimester, and under predicted (P≤0.05) during the 3rd trimester of pregnancy. A second evaluation was carried out assuming thermal neutral (TN) conditions. In this case, it was found that during the 2nd and 3rd trimesters there was an over prediction (P≤0.05) of ADG relative to observed. Under these same TN conditions, the ad libitum intake of these cows was under predicted (P≤0.05) for both the 2nd and 3rd trimesters. These results suggest current energy equations for modelling environmental stress, over predict maintenance requirements for wintering beef cows in western Canada. The results also suggest that the cows experienced some degree of cold stress, but not as severe as modelled by the NRC (1996) equations. Further research is required to more accurately model cold stress felt by mature cattle, and their ability to acclimatise to western Canadian winter conditions.

A trial was conducted to evaluate the accuracy of the 1996 NCR beef model to predict DMI and ADG of pregnant cows under western Canadian conditions. Over two consecutive years, 90 Angus (587±147 kg) cows assigned to 15 pens (N=6) were fed typical diets ad libitum, formulated to stage of pregnancy. Data collection included pen DMI and ADG (corrected for pregnancy), calving date, calf weight, body condition scores and ultrasound fat measurements, weekly feed samples and daily ambient temperature. DMI and ADG for each pen of cows in each trimester was predicted using the computer program Cowbytes based on the 1996 NRC beef model. The results indicate that in the 2nd and 3rd trimester of both years the model under predicted (P≤0.05) ADG based on observed DMI. Ad libitum intake was over predicted (P≤0.05) during the 2nd trimester, and under predicted (P≤0.05) during the 3rd trimester of pregnancy. A second evaluation was carried out assuming thermal neutral (TN) conditions. In this case, it was found that during the 2nd and 3rd trimesters there was an over prediction (P≤0.05) of ADG relative to observed. Under these same TN conditions, the ad libitum intake of these cows was under predicted (P≤0.05) for both the 2nd and 3rd trimesters. These results suggest current energy equations for modelling environmental stress, over predict maintenance requirements for wintering beef cows in western Canada. The results also suggest that the cows experienced some degree of cold stress, but not as severe as modelled by the NRC (1996) equations. Further research is required to more accurately model cold stress felt by mature cattle, and their ability to acclimatise to western Canadian winter conditions.

Two experiments were conducted to determine efficacy of GnRH analogs, Cystorelin (CYS, gonadorelin diacetate tytrahydrate) and Factrel (FAC, gonadorelin hydrochloride), for use in beef timed AI synchronization. In Experiment one 342 beef cows from 7 herds were assigned CYS or FAC treatment as part of the Ovsynch protocol (GnRH d 0 and 9, Lutalyse d 7). Cattle treated with FAC had greater tendency (P=.09) to be pregnant at d 45. One individual herd demonstrated FAC-treated cows had more pregnancies at day 45. In Experiment two, 18 beef cows received either CYS or FAC as part of the Ovsynch protocol, intensive blood samples, from time -30 to 525 min post GnRH, were collected at each GnRH injection. Ultrasounds were conducted daily over the course of the protocol. A treatment by phase interaction (P=.03) was found for the time to maximum LH concentration, where CYS-treated follicular cows had a shorter interval than did FAC treated follicular or luteal cows. The duration of detectable LH response showed a treatment by phase interaction (P = .02) where follicular and luteal CYS-treated cows had shorter interval than follicular or luteal FAC-treated cows. The variables maximum LH concentration, and area under LH curve did not differ. Cows treated with CYS had more (P=.02) non-dominant follicles. In Experiment three, 16 ewes randomly received either CYS, FAT or Fertagyl (FER; gonadorelin diacetaate tytrahydrate), and FAT's induced LH maximum concentration occurred sooner (P=.02) than CYS. We conclude that either product may be used in beef cows without compromising fertility. / Master of Science

Over 2 years (Year 1, 2009-2010; Year 2, 2010-2011), two separate experiments were conducted to evaluate the effects of winter feeding system (n=3) on beef cow performance, reproductive performance, economics and forage degradability. The three systems (treatments) were grazing pea crop residue (PEA) cv. ‘Performance 40-10’ (Year 1, TDN = 50.2%, CP = 7.3%; Year 2, TDN = 56.9%, CP = 8.9%) in field paddocks, grazing oat crop residue (OAT) cv. ‘Baler’ (Year 1, TDN = 59.1%, CP = 2.9%; Year 2, TDN = 66.9%, CP = 5.3%) in field paddocks, and feeding mixed grass-legume hay in drylot pens (DL) (Year 1, TDN = 61.4%; CP = 8.8%; Year 2, TDN = 52.3%, CP = 12.3%). In the first experiment, 90 dry, pregnant Black Angus cows (Year 1, 629 kg ± 74 kg; Year 2, 665 ± 69 kg) stratified by body weight (BW) and days pregnant were randomly allocated to 1 of the 3 systems. Cows were allocated feed in the field or pen on a 3 d basis and supplemented oat grain daily at 0.4-0.6% BW depending on environmental conditions. Dry matter intake (DMI) was estimated for each system using the herbage weight disappearance method. Cow BW, body condition score (BCS), and rib and rump fat were measured at start and end of trial and cow BW was corrected for conceptus gain based on calving data. When data from the first 20 d were pooled over 2 years, initial cow BW was greater (P < 0.01) for the DL and OAT cows compared to the PEA cows and final cow BW was different (P < 0.01) between all 3 winter feeding systems. The change in BW was also greater (P < 0.01) for DL cows compared to cows on the OAT and PEA treatments. Analysis of the first 20 d of Year 1 study period and the total Year 2 study period, showed a significant (P < 0.01) year by treatment interaction for final BW and BW change. The differences (P < 0.01) in initial BW, final BW and BW change between the first 20 d of Year 1 study period and the total Year 2 study period (20 d) suggest feed quality, animal preference and weather conditions may cause difficulties when grazing residues in winter grazing systems. Analysis of the entire trial period in Year 1 (62 d) indicates differences (P < 0.01) for final BW and BW change between cows on all three systems. The change in rib and rump fat was also different (P < 0.01) between cows in all 3 systems. In Year 2 (20 d), initial BW, final BW and BW change were different (P < 0.01) between DL and PEA cows, and between (P < 0.01) OAT and PEA cows. No difference (P > 0.05) was found for cow rib and rump fat in Year 2 and no difference (P > 0.05) was found for BCS in either Year 1 or Year 2 for cows managed in all 3 systems. Differences (P < 0.05) were observed for calving rate and calf birth weight between the DL and OAT system cows, but not between (P > 0.05) cows managed in the DL and PEA or OAT and PEA systems. Costs per cow per day were $1.22, $1.01 and $2.77 for PEA, OAT and DL systems in Year 1, respectively. In Year 2, cow costs per day were $1.59, $1.44 and $1.84 for PEA, OAT and DL systems, respectively. In experiment 2, three ruminally cannulated, dry Holstein cows were fed a silage based total mixed ration (TMR) of 22 kg barley silage, 7 kg chopped alfalfa hay and 1 kg energy supplement (DAC-485). In-situ degradability was studied to determine the extent of degradation of pea, oat and grass-legume hay collected at start (SOT) and end of test (EOT) in experiment one. Rate of degradation (Kd) of DM was greater (P < 0.01) for PEA EOT compared to HAY, OAT SOT and OAT EOT. Dry matter rate of degradation for PEA SOT was greater (P < 0.05) compared to OAT SOT and OAT EOT. The effectively degradable fraction of CP was greater (P = 0.03) for HAY compared to PEA EOT. The ruminally undegradable fraction of CP was greater (P = 0.03) for PEA EOT compared to HAY. Acid detergent fiber rate of degradation (Kd) was greater (P = 0.01) for PEA EOT compared to HAY, OAT SOT and OAT EOT. Acid detergent fiber rate of degradation for PEA SOT was greater (P < 0.05) compared to OAT SOT and OAT EOT. No differences (P > 0.05) were observed between either OAT SOT and OAT EOT or PEA SOT and PEA EOT for S, D, U, ED or RU suggesting that weathering did not have an effect on the degradability of the forages. The results of these experiments show that it is possible to maintain cow BW through the winter months in Western Canada by grazing oat crop residues, which have the potential to reduce winter feeding costs.

Johne’s disease (JD) in cattle is a disease of economic importance caused by Mycobacterium avium subspecies paratuberculosis (MAP). Studies were conducted to estimate the losses due to lower weaning weight of beef calves from MAP test-positive dams, to compare the perceptions of producers and veterinarians on the burden and economic aspects of MAP infection in cow-calf herds, and to evaluate whether testing and culling MAP test-positive cows is economically beneficial. Calves from cows with strong-positive ELISA results were 21.5 kg lighter at weaning compared to calves from ELISA-negative cows. Calves from heavy MAP shedding cows were 58.5 kg lighter, and calves from moderate shedders were 40.8 kg lighter compared to the calves from fecal-culture negative cows. Based on average feeder calf value during 2007 to 2012, these losses corresponded to US $57 per calf for ELISA strong-positive dams, US $157 per calf for heavy fecal shedder dams, and US $109 per calf for a moderate fecal shedder dam. Seedstock producers and the producers enrolled in control programs were more likely to have MAP uninfected herds. The average prevalence reported by producers was 0.8%. Compared to the small herds (<50 head), the average test-positive percentages and estimated prevalences were reported to be higher in medium (50-149) and highest in large (≥150) herds. Veterinarians reported an overall animal level prevalence in their client herds of 5%. Seedstock herds had a lower prevalence and these producers were more likely to enroll in a JD control program. Income lost due to the presence of JD in an infected cattle herd was perceived to be higher by veterinarians. Compared to the veterinarians, seedstock producers were more likely to perceive genetic losses due to culling MAP positive cows. Average annual loss due to JD in a 100 cow herd with a 7% MAP prevalence was $1,644 and $1,747 based on information provided by producers and veterinarians, respectively. Herd level production decreased with increasing prevalence. Compared to test and cull after ELISA or ELISA followed by fecal culture, using fecal culture alone provided the fastest reduction in herd prevalence. Fecal culture was also the least costly alternative based on long-term cumulative costs of an annual test and cull program. Results from the current study suggest that although testing provides faster progress, limiting within herd transmission by sale of all weaned calves and purchasing only low-risk replacements can also reduce prevalence. Results suggest that MAP infection in cows causes significant losses for the calves that are produced. While the knowledge about JD varied between producers and veterinarians, seedstock producers were more enthusiastic about MAP control programs and had lower MAP prevalence in their herds. Overall losses due to MAP infection in the herd might be substantial. It is very costly to control or eliminate MAP once the infection is established in a herd.

The beef cattle industry has placed increased focus on mature cow size as a result of its influence on production efficiency and profitability. The objectives of this study were to evaluate relationships among lifetime measures of body weight (BW) and frame score (FS) in commercial beef females, and to assess the value of immature measures as predictors of mature cow size. Measurements of BW, hip height (HH), body condition score (BCS), and calculated FS were recorded at weaning (WN), breeding at 13 mo age (BR), and 8 subsequent periods, ceasing at approximately 5 yr of age for 232 Angus-cross females born 2004 through 2008. Correlation analysis revealed significant (P < 0.001) relationships among BW taken at WN and BR with BW measurements taken at 2.5, 3.8, and 4.8 yr of age (WN r = 0.70, 0.51, 0.61; BR r = 0.65, 0.57, 0.64, respectively). Significant relationships (P < 0.001) existed between FS collected at WN and BR, and FS at 2.5 and 3.8 yr (WN= 0.70, 0.72; BR= 0.79, 0.82, respectively). Repeatability of lifetime FS measures was 0.73. BCS was a significant (P < 0.001) source of variation in mature BW, with a unit change in BCS accounting for 41 kg BW change at 4.8 yr (P < 0.001). BW and FS were moderately to strongly related (P < 0.001) at WN, BR, 2.5, 3.8, and 4.8 yr (r = 0.62, 0.49, 0.62, 0.62, and 0.47 respectively). Prediction models for BW at 4.8 yr were similar using weaning BW alone, or with inclusion of both weaning BW and HH (R2 = 0.57 and 0.56). Similarly, breeding BW and HH were non-additive for prediction of 4.8 yr BW (R2 = 0.68, 0.58, and 0.68 for BW, HH, and BW +HH respectively). Performance at immature ages proves to be a satisfactory indicator of mature size, supporting continued incorporation of immature BW and HH and/or FS measurements into selection practices. / Master of Science

Orientador: Sandra Aidar de Queiroz / Coorientador: Roberto Carvalheiro / Banca: João Ademir de Oliveira / Banca: Patrícia Tholon / Resumo: No Brasil, são escassos os estudos referentes ao escore de condição corporal (ECC) de vacas de corte que, quando associado ao peso, têm impacto na produção de bezerros e na reprodução, pois reflete o balanço energético do corpo animal. Assim, o objetivo deste trabalho foi associar ECC com a prenhez de vacas Nelore e com o desempenho de seus produtos a desmama, bem como verificar se esta característica pode ser incluída nos critérios de seleção da raça Nelore. O ECC das vacas foi atribuído no diagnóstico de gestação ou no desmame, variando de 1 a 5. Por regressão logística, utilizando o método de seleção Stepwise, foram analisados os seguintes efeitos sobre o ECC: grupo de contemporâneos a desmama, peso e altura de garupa da vaca, ordem de parto, diagnóstico de gestação, histórico reprodutivo anterior à coleta do ECC, presença de bezerro ao pé e escores de C e P dos produtos a desmama. Na análise genética, elaborada usando-se inferência baeysiana, foram incluídos os mesmos efeitos anteriores, com peso e altura de garupa da vaca como co-variáveis, e acrescidos do efeito genético aditivo da vaca. Os resultados mostraram que vacas com ECC elevado emprenharam mais do que as de pior ECC, porém o desempenho dos seus bezerros foi inferior. Vacas multíparas foram mais pesadas e de escores mais magros (ECC 1 e 2). As correlações de Spearman entre ECC e suas fontes de variação foram, em sua maioria, de baixa magnitude. Os efeitos que mais explicaram a variação do ECC foram peso, grupo de contemporâneos e altura da garupa. A estimativa de herdabilidade do ECC foi igual a 0,22, com intervalo de credibilidade entre 0,13 e 0,31, valor de magnitude moderada, demonstrando a presença de ação gênica aditiva na determinação do ECC e que este pode ser utilizado como critério de seleção de vacas / Abstract: In Brazil few studies have been done on the body condition score (BCS) of beef cows. The knowledge of this score associated with body weight can produce an impact on calf production and cow's reproduction, because it reflects the energy balance of the animal body. The objective of this study was to associate BSC with pregnancy of cows and the performance of their calves at weaning, and to verify if this trait could be included as selection criteria. The BCS was recorded on pregnancy diagnosis or at weaning and ranged from 1 to 5. Applying logistic regression, using the Stepwise selection method, we analyzed the following effects on BSC: contemporary group at weaning, classes of weight and hip height of the cow, number of calvings, pregnancy diagnosis, reproductive history prior to collection BSC, the presence of calf and C and P score of calves at weaning. For the genetic analysis, performed using Baesyan inference, the same effects were considered, with weight and hip height as covariate, and also the genetic direct effect of the cow. The results showed that cows with higher BCS had better pregnancy rate than those with lower BSC, but the performance of their calves was worse. Multiparous cows were heavier and had leaner scores (BCS 1 and 2) than the primiparous ones. The Spearman rank correlation between BSC and their sources of variation were generally of low magnitude. Contemporary group, body weight and height of the cow were the effects that accounted for most of the variation of BSC. The heritability estimate of BCS was equal to 0.22 with credible interval ranging from 0.13 to 0.31, a moderate magnitude value that indicates the presence of additive genetic variation and that BSC can be used as selection criterion for cows / Mestre

Identifying molecular mechanisms regulating cellular energy utilization may lead to increased understanding of maintenance energy cost and improved feed efficiency in beef cows. Three experiments were conducted to characterize measures of residual feed intake (RFI) in pregnant beef cows; to examine the effects of moderate dietary restriction on visceral organ mass and proteins relating to energy metabolism; and to investigate the influence of pregnancy on visceral organ mass and proteins relating to energy metabolism. The first experiment combined data from five experiments using 321 pregnant Angus × Simmental cows. Including ultrasound fat measures and diet/management information increased the feed intake prediction model R2 by 7.3% and > 20%, respectively. Individual experiment RFI models varied greatly in accuracy. In the second experiment, 22 pregnant beef cows were fed at 85% (LOW; n=11) or 140% (HIGH; n=11) of net energy requirements during mid- to late-gestation. Tissue samples from liver, kidney, muscle, ruminal papillae, pancreas, and small intestinal muscosa were collected. Western blots were conducted to quantify abundance of: proliferating cell nuclear antigen, ATP synthase, ubiquitin, and Na/K+ ATPase for all tissues; peroxisome proliferator-activated receptor gamma, peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α), and 5’-adenosine monophosphate-activated protein kinase and phosphorylated-AMPK (pAMPK) for liver, muscle, and rumen; phosphoenolpyruvate carboxykinase for liver and kidney; and uncoupling protein 2 for liver. Cows fed HIGH had greater (P ≤ 0.04) ADG and final BW than cows fed LOW. Ubiquitin abundance in muscle was greater (P = 0.009) in cows fed LOW, and PCG-1α in liver was greater (P = 0.03) in cows fed HIGH. In the third experiment, 18 pregnant (PREG; n =9) or non-pregnant (OPEN; n=9) Angus × Simmental cows were fed for ad libitum intake during mid- to late-gestation. Tissues were weighed and collected and analyzed for protein abundance as described in the second experiment. Liver mass was lower (P ≤ 0.02), abundance of Na+/K+-ATPase was greater (P =0.04) and rumen pAMPK abundance was increased (P = 0.006) in PREG cows. These experiments indicate that measuring RFI in pregnant cows may pose some challenges, and nutrient restriction and pregnancy can influence molecular factors influencing feed efficiency. / Financial support was provided by Canadian Beef Cattle Industry Science Cluster, through funding provided by the Beef Cattle Research Council and Agriculture and Agri-Food Canada, Agriculture Adaptation Council-Farm Innovation Program, Ontario Ministry of Agriculture, Food and Rural Affairs, and the Ontario Cattleman’s Association.

Made available in DSpace on 2014-06-11T19:26:06Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-02-28Bitstream added on 2014-06-13T18:29:27Z : No. of bitstreams: 1 fernandes_afa_me_jabo.pdf: 322816 bytes, checksum: d60d045e58509fb91c9a8dc47345094d (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / No Brasil, são escassos os estudos referentes ao escore de condição corporal (ECC) de vacas de corte que, quando associado ao peso, têm impacto na produção de bezerros e na reprodução, pois reflete o balanço energético do corpo animal. Assim, o objetivo deste trabalho foi associar ECC com a prenhez de vacas Nelore e com o desempenho de seus produtos a desmama, bem como verificar se esta característica pode ser incluída nos critérios de seleção da raça Nelore. O ECC das vacas foi atribuído no diagnóstico de gestação ou no desmame, variando de 1 a 5. Por regressão logística, utilizando o método de seleção Stepwise, foram analisados os seguintes efeitos sobre o ECC: grupo de contemporâneos a desmama, peso e altura de garupa da vaca, ordem de parto, diagnóstico de gestação, histórico reprodutivo anterior à coleta do ECC, presença de bezerro ao pé e escores de C e P dos produtos a desmama. Na análise genética, elaborada usando-se inferência baeysiana, foram incluídos os mesmos efeitos anteriores, com peso e altura de garupa da vaca como co-variáveis, e acrescidos do efeito genético aditivo da vaca. Os resultados mostraram que vacas com ECC elevado emprenharam mais do que as de pior ECC, porém o desempenho dos seus bezerros foi inferior. Vacas multíparas foram mais pesadas e de escores mais magros (ECC 1 e 2). As correlações de Spearman entre ECC e suas fontes de variação foram, em sua maioria, de baixa magnitude. Os efeitos que mais explicaram a variação do ECC foram peso, grupo de contemporâneos e altura da garupa. A estimativa de herdabilidade do ECC foi igual a 0,22, com intervalo de credibilidade entre 0,13 e 0,31, valor de magnitude moderada, demonstrando a presença de ação gênica aditiva na determinação do ECC e que este pode ser utilizado como critério de seleção de vacas / In Brazil few studies have been done on the body condition score (BCS) of beef cows. The knowledge of this score associated with body weight can produce an impact on calf production and cow´s reproduction, because it reflects the energy balance of the animal body. The objective of this study was to associate BSC with pregnancy of cows and the performance of their calves at weaning, and to verify if this trait could be included as selection criteria. The BCS was recorded on pregnancy diagnosis or at weaning and ranged from 1 to 5. Applying logistic regression, using the Stepwise selection method, we analyzed the following effects on BSC: contemporary group at weaning, classes of weight and hip height of the cow, number of calvings, pregnancy diagnosis, reproductive history prior to collection BSC, the presence of calf and C and P score of calves at weaning. For the genetic analysis, performed using Baesyan inference, the same effects were considered, with weight and hip height as covariate, and also the genetic direct effect of the cow. The results showed that cows with higher BCS had better pregnancy rate than those with lower BSC, but the performance of their calves was worse. Multiparous cows were heavier and had leaner scores (BCS 1 and 2) than the primiparous ones. The Spearman rank correlation between BSC and their sources of variation were generally of low magnitude. Contemporary group, body weight and height of the cow were the effects that accounted for most of the variation of BSC. The heritability estimate of BCS was equal to 0.22 with credible interval ranging from 0.13 to 0.31, a moderate magnitude value that indicates the presence of additive genetic variation and that BSC can be used as selection criterion for cows

Doctor of Philosophy / Department of Animal Sciences and Industry / Jeffrey S. Stevenson / Four experiments were conducted in beef cows to determine factors that increased the probability of pregnancy per AI when cows are inseminated by appointment. Cows in all experiments were inseminated after a 7-d CO-Synch + CIDR program (100 μg GnRH [2 mL Factrel, Pfizer Animal Health, Whitehouse Station, NJ] 7 d before 25 mg PGF₂[subscript]α [d 0; 5 mL Lutalyse; Pfizer Animal Health]). Experiment 1 compared 1 vs. 2 inseminations and GnRH injection times at 60 and 75 h after the CO-Synch + CIDR program. Delaying AI until 75 h, according to interpretation of estrus-detection patches, for cows not in estrus by 60 h after CIDR insert removal increased (P < 0.05) pregnancy risk (PR) compared with cows not in estrus and inseminated at 60 h (51.4 vs. 41.7%), respectively. The necessity of GnRH injection concurrent with AI was tested in experiment 2. Cows displaying estrus by 65 h that were injected with GnRH had similar PR to cows in estrus and not treated with GnRH (61.9 vs. 60.4%), respectively. Cows in experiment 2 that did not display estrus, but were treated with a GnRH injection at 65 h and then inseminated at 84 h after CIDR insert removal had increased PR compared with similar cows not treated with GnRH (33.4 vs. 15.0%; P < 0.01), respectively. Experiments 3 and 4 were observational studies conducted to determine if blood metabolites glucose and beta-hydroxy butyrate (BHB experiment 3), or physical body and blood metabolites, (glucose, BHB, non-esterified fatty acids [NEFA], blood urea nitrogen [BUN], body weight, rump fat [RF], or BCS; experiment 4) were indicative of future reproductive success in suckled beef cows enrolled in a timed AI program. In experiment 3, plasma glucose concentration 10 d before AI was lesser (P = 0.01; 52.2 vs. 56.9 mg/dL) and serum BHB concentration was lesser (P < 0.01) in cows that became pregnant 35 d after timed AI than for cows that did not become pregnant (600 vs. 690 μM), respectively. Experiment 4 identified relationships between indicators and reproductive success including the finding that serum NEFA concentration 2 to 4 wk before AI is negatively correlated (P < 0.05) with PR to AI.