Thesis (PhD)--University of Stellenbosch, 2003. / ENGLISH ABSTRACT: The geographic genetic population structure and evolutionary history of the African four-striped mouse, Rhabdomys pumilio, was investigated using mitochondrial (mtDNA) cytochrome b gene (1140 bp) and control region (994 bp) sequences and a combination of cytogenetic banding techniques (G- and C-banding), and fluorescence in situ hybridization. Two cytotypes (2n = 46 and 2n = 48) were identified by cytogenetic analysis. No evidence of diploid number variation within populations was found nor were there differences in gross chromosome morphology, or subtle interchromosomal rearrangements at levels detected by ZOO-FISH. The comparative painting data (using the complete suite, N = 20, of Mus musculus chromosome specific painting probes) show that 10 mouse chromosomes have been retained as chromosomal arms, or intact chromosome blocks within the R. pumilio genome, six produced double signals, while the remaining four hybridized to three or more R. pumilio chromosomes. In total, the 20 mouse chromosome paints detected 40 segments of conserved synteny. Their analysis revealed eight R. pumilio specific contiguous segment associations, a further two that were shared by R. pumilio and other rodents for which comparable data are available, the Black (Rattus rattus) and Norwegian (Rattus nONegicus) rats, but not by the Chinese hamster, Cricetulus grise us. The results suggest that mouse chromosomes 1, 10, and 17 have undergone extensive rearrangements during genome evolution in the murids and may be useful markers for enhancing our understanding of the mode and tempo of chromosome evolution in rodents. Following initial studies using control region sequences, the phylogeographic appraisal of R. pumilio was done using cytochrome b gene sequences. Analyses based on a variety of analytical procedures resulted in the detection of two major mtDNA lineages that correspond roughly to the xeric and mesic biotic zones of southern Africa. One clade comprises specimens with 2n = 48, and the other representatives of two cytotypes (2n = 48 and 2n = 46). The mean sequence divergence (12.0%, range 8.3% -15.6%) separating the two mtDNA clades is comparable to among-species variation within murid genera suggesting their recognition as distinct species, the prior names for which would be R. dilecfus and R. pumilio. Low sequence divergences and the diploid number dichotomy within the mesic lineage support the recognition of two subspecies corresponding to R. d. dilecfus (2n = 46) and R. d. chakae (2n = 48). The data do not support subspecific division within the nominate, R. pumilio. Molecular dating places cladogenesis of the two putative species at less than 5 million years, a period characterised by extensive climatic oscillations which are thought to have resulted in habitat fragmentation throughout much of the species' range. / AFRIKAANSE OPSOMMING: Die geografiesebevolkingsstruktuur en evolusionêre verwantskappe binne die Afrika streepmuis, Rhabdoys pumilio, is ondersoek deur middel van mitochondriale ONS volgordebepaling van die geenfragment sitochroom b (1140 basispare) en die reguleerstreek (994 bp) in kombinasie met sitogenetiese tegnieke (G- en Cbandkleuring en f1uoreseerende in situ hibridisasie). Twee sitotipes (2n = 46 en 2n = 48) is geidentifiseer deur sitogenetiese analasie. Geen bewys van variasie in die 2n chromosoomgetal binne bevolkings is gevind nie. Verder is daar ook geen verskil in die morfologies struktuur van chromosome aanwesig binne bevolkings nie. Vergelykende data (verkry met behulp van die N = 20 Mus musculus chromosoomspesifiekepeilers) dui daarop dat 10 muis chromosome behoud gebly het as chromosoomarms of chromosoomblokke binne die R. pumilio genoom. Ses peilers het dubbel seine gelewer terwyl die oorblywende vier peilers gehibridiseer het aan drie of meer R. pumilio chromosome. In totaal het die 20 muischromosoomverwe 40 konserwatiewe segmente geidentifiseer. Die analise dui agt R. pumilio spesifieke aaneenlopende segmentassosiasies aan, met 'n addisionele twee wat deur R. pumilio en ander muisagtiges vir wie vergelykende data beskikbaar is, byvoorbeeld die swart (Rattus rattus) en Noorweegse (R. norvegicus) rot maar nie die Chinese hamster, Cricetulus grise us, gedeel word. Die resultate stel voor dat muischromosoom 1, 10 en 17 ekstensiewe herrangskikkings ondergaan het gedurende die genoom evolusie binne die Muridae en dat hulle waarskynlik waardevolle merkers kan wees om beide die patroon en tempo van chromosome evolusie in muisagtiges verder te kan verstaan. Die filogeografiese verwantskappe binne R. pumilio is ondersoek deur middel van ONS volgordebepalings van die reguleerstreek asook sitochroom b. Die resultate van hierdie studie het twee divergente mitochondriale ONS eenhede ontdek wat gekorreleer kan word met xeriese en mesiese klimaatsones binne suidelike Afrika. Een groep bestaan uit diere met 2n = 48, terwyl die ander genetiese groep twee sitotipes (2n = 46 en 2n= 48) insluit. 'n Gemiddelde genetiese divergensie van 12.0% (varieer tussen 8.3% - 15.5%) verdeel die twee mtDNS-groepe en is vergelykbaar met tussenspesievariasie binne ander muisagtige genera, wat moontlik daarop dui dat twee verskillende spesies teenwoordig is; die voorgestelde name is R. di/ectus en R. pumilio. Lae genetiese divergensie binne die mesiese groep versterk die moontlike teenwoordigheid van twee subspesies, R. d. di/ectus (2n = 46) en R. d. chakae (2n = 48). Die data verleen egter nie steun aan die divisie binne R. pumilio nie. Molekulêre datering van die twee spesies dui daarop dat die divergensie ten minste 5 miljoen jaar gelede plaasgevind het. Die periode was gekarakteriseer deur ekstensiewe klimaatsossilasies, wat gely het tot habitat fragmentasie in die spesie se verspreidingsgebied.

published_or_final_version / abstract / Statistics and Actuarial Science / Master / Master of Philosophy

published_or_final_version / abstract / Economics and Finance / Doctoral / Doctor of Philosophy

published_or_final_version / Statistics and Actuarial Science / Master / Master of Philosophy

Mosquitoes pose a serious threat to the economy, health status, and biosecurity of countries around the world. Mosquitoes kill an average of 700,000 people per year. The global expansion of air, sea, and land transport networks has greatly enhanced the spread of mosquitoes internationally. In the Pacific, the number of mosquito-borne diseases occurring has been on the rise in recent years, possibly as a result of human-mediated dispersal of larvae and adult mosquitoes. The Kingdom of Tonga has had numerous outbreaks of dengue fever and chikungunya virus in recent years. Previous research has catalogued species occurrences and distributions throughout Tonga. However, it is unknown whether new species have arrived in Tonga, and if distribution of previously found species has changed since the last comprehensive survey in 2006. Present research aims to update the literature by conducting a mosquito survey at 84 sites across the four islands of Tongatapu, Pangaimotu, ‘Oneata, and ‘Eua to record the distribution and occurrence of mosquito larvae. Nine mosquito species were collected: Aedes aegypti Linnaeus, A. albopictus Skuse, A. tongae Edwards, A. horrescens Edwards, A. vexans nocturnus Theobold, Culex annulirostris Skuse, C. albinervis Edwards, C. quinquefasciatus Say and C. sitiens Wiedemann. The collection of A. albopictus is the second time that this species has been recorded in Tonga. Moreover, the spatial extent of this species throughout Tonga was far greater than previously recorded. A major outcome of this survey has been the creation of an identification key for the mosquito larvae species of Tonga. This key should increase the accuracy of positive mosquito larvae identifications in Tonga. Mosquitoes were more frequently collected in artificial (e.g., used car tyres, fuel drums, containers) than natural (e.g., pools, ponds, tree holes) habitats. Car tyres, water containers, fuel drums, fridges, washing machines, and ponds were the most common habitats in which mosquito larvae were found. Aedes aegypti, A. albopictus, and C. quinquefasciatus were the three most common mosquito species collected, whereas A. tongae, A. horrescens, A. vexans nocturnus, C. annulirostris, C. sitiens, and C. albinervis were less frequently found. Multiple logistic regression analyses indicated that habitat volume had a significant positive effect on the presence of A. albopictus and A. tongae, whereas conductivity had a significant positive effect on the presence of C. annulirostris. Additionally, the volume by temperature interaction was a significant predictor of species presence for A. aegypti, A. albopictus, and C. annulirostris (as habitat volume increases, the effect of temperature went from neutral to negative). This suggests that larger, cooler habitats favour colonisation by these species. The number of artificial habitats (particularly used car tyres) present may have significantly increased since previous studies. Management should therefore focus on implementing community-run mosquito projects aimed at reducing the number of artificial habitats capable of being colonised by mosquito larvae. Covering, tipping out water, and infilling these habitats with soil to prevent mosquito oviposition is a pragmatic and straightforward mosquito control solution. This should immensely reduce the abundance of mosquitoes and may prevent disease outbreak in Tonga.

Recently, the conventional Matrix Converter has been considered for aerospace applications because of its compactness in weight and size due to absence of a DC-Link capacitor. In addition the Matrix Converter is capable of producing a variable output voltage with unrestricted input and output frequency. This is useful in aerospace applications which require a wide range of input frequency, sometimes from 360 Hz to 900 Hz. These key features are only obtained from the Matrix Converter without using any large passive components. The Matrix Converter has an inherent regeneration capability. However, the avoidance of the regeneration may be vital in many aerospace applications, such as aircraft surface actuation systems. According to current aircraft power quality specifications regeneration is not allowed and must be dissipated with in the Matrix Converter drive itself. This thesis proposes two novel methods which allow regeneration from the Matrix Converter motor drive to be avoided. These are the Bi-Directional Switch (BDS) method and Input Power Clamp (IPC) method. In order to detect regeneration in the Matrix Converter motor drive two techniques are used. These are the Power Comparison (PC) technique and Input Voltage Reference (IVR) technique. Finally, to validate the proposed methods a Matrix Converter has been designed and built with a Regeneration Control Circuit (RCC). Indirect vector control is used to control 4.0 kW Induction Motor. Based on the simulation results and experimental results using the BDS method to avoid regeneration with a Matrix Converter is feasible.

While solar photovoltaic (PV) panels can offset fuel, emissions, and water use at the power plant, high levels of installed rooftop PV capacity can have negative impacts on the stability and efficiency of the local grid because of power factor (PF) degradation. Specifically, electric utilities have noted voltage fluctuations from solar PV that occur more dynamically than legacy, electromechanical voltage regulation solutions like capacitor banks are designed to correct. At the same time, distributed power electronics devices like inverters can provide the type of dynamic voltage support that utilities seek to maintain reliability while juggling load growth and a greening grid. Using data from Pecan Street Inc. Smart Grid Demonstration Project in Austin, Texas and Austin Water Utility (AWU), this research examines the potential for a three-phase, four quadrant variable speed drive (VSD) in the water sector to provide PF correction to a load pocket of 63 homes experiencing varying levels of PV penetration. In the analysis, the VSD is not reserved for voltage support; instead it is predominantly used to drive a 30 kilowatt (kW) centrifugal sewage pump. The simulation determines the degree to which the VSD can restore PF to a threshold of 0.95, slightly below the targeted value for Austin's electric utility. Further, the economic viability of using the VSD as a grid device is explored by comparing the per unit cost of the PF correction it provides to a current utility solution for dynamic voltage regulation. / text

The purpose of this research is to develop a dataset for some of the most important fire characteristics, namely CO2 yield, CO yield, HCN yield, soot yield and heat of combustion for probabilistic analysis and modelling. Raw data in time series are required to mechanically reduce experimental data into yields (kg/kg) and effective heats of combustion (MJ/kg), which are expressions for the amount of products generated per unit mass of fuel. Mass loss rate thresholds were applied to all tests to define the beginning and end of tests. These species yields and heat of combustions were then grouped by material compositions and fitted with distribution functions to produce distributions curves. As fire species productions and heat of combustions are dependent on the fire conditions as it develops, different yields are expected at different fire stages. These have been identified as the growth (G), transition (T), and smouldering (S) stages in this research. These values are also compared against, and are generally in agreement with, other research data. Nonetheless, some discrepancies have occurred and require further information to ascertain the material characteristics and combustion conditions. In conclusion, design recommendations for these fire characteristics have been made for several material groupings and verified against other research results. Certain physical and chemical limitations exist for combustions and have not been reflected in the fitted distribution, including stoichiometric yields and unlimited air yields. As such, species yields and heat of combustions beyond these values should not be considered in fire engineering design and analysis. Research results on HCN including all required data parameters for yield conversions were difficult to obtain and require further research efforts. Tube furnace results were initially investigated. Unfortunately, without a continuous mass record, has proved to be challenging in producing reliable mass loss rate profiles for yield conversions. A semi-automated data reduction application UCFIRE was also used. However, certain technical difficulties were encountered and require modifications to broaden its applicability.

The advent of cheaper, more powerful substation relays, now commonly known as Intelligent Electronic Devices (IEDs), will have a substantial impact on Powerco information systems. Their impact is two-fold; the sheer volume of information associated with modern relays will require a more capable relay management system than the one currently being used. The second effect is the amount of data that can be gathered and used for network improvement. Improper management of both types of information will lead to worse outcomes for Powerco reliability performance, and ultimately its financial performance. This report details two projects concerned with the management of IED information.The first project is concerned with a proposed upgrade of the Powerco relay management system. This involved requirements engineering, investigation of database systems and a commercial tendering process. It was found that the data schema had a large effect on efficiency and efficacy of relay management systems. The optimal solution would be to procure a proven relay management system from an established vendor, given resource constraints and proven-for-purpose data schema. The second project is focused on the deployment of automated event report collection software. The impetus of this project was the inefficiencies that arise from manual collection and processing of network event data from substation relays. These hamper the network improvement process, negatively affecting Powerco network reliability. A Cost-Benefit Analysis was performed to determine if such functionality was worth deploying across the network. It was found that automated event report collection produced operational savings from reduced technician dispatch frequency. Other benefits include greater data captured, faster fault response time, and a transformation of the existing reactive network improvement engineering process to one that is proactive. This will require additional engineering resources to be dedicated to event analysis. A deployment schedule was formulated based on historical reliability and agreed service levels. The larger implications of this report are that additional resourcing, and careful consideration of the information management processes are required to take advantage of the greater volume of data generated by IEDs.

No description available.