A Baía de Guanabara, ambiente de localização do Arsenal de Marinha do Rio de Janeiro (AMRJ), sofre com a intensa pressão antrópica, principalmente no aspecto da qualidade das águas. Neste contexto, uma fração considerável da poluição decorre das atividades dos estaleiros, uma vez que a atividade industrial naval no Rio de Janeiro não tem mecanismo de controle de poluição à altura do seu real potencial poluidor. Esta ausência de fiscalização possibilita o lançamento na Baía de resíduos sólidos, esgotos sanitários, efluentes químicos, oleosos e tóxicos, tornando crescente a contaminação dessas águas, margens e mangues. Estes descartes cada vez mais são alvo das exigências ambientais da sociedade e das legislações. Devido a isto, a gestão de efluentes líquidos do Arsenal tornou-se prioritária, para tal, esse estudo foi proposto, tendo iniciado pela análise das oficinas do estaleiro, na qual as operações no dique foram identificadas como uma das mais impactantes do estaleiro. A partir desta constatação, estão apresentadas duas fontes de pesquisas para a redução dos impactos. Na primeira etapa, há o estudo das atividades geradoras de efluentes no dique de reparo, com os objetivos de propor a implantação das diretrizes de melhores práticas de gestão, de minimizar a geração de efluentes líquidos e de contribuir para a adoção de práticas ambientais proativas. Como segunda pesquisa, com base nas tecnologias mundiais, há a proposta de tratamento dos efluentes de um dique, na qual foram identificados os processos que irão atender às necessidades ambientais do estaleiro, com as opções de escolha entre o tratamento parcial, para o descarte na rede pública, ou com o prosseguimento do processo até o seu reúso. As conclusões deste estudo apontam para a implantação da gestão ambiental do dique sistematizada, rigorosa e integrada com a gestão das embarcações, acrescentando-se a isto, as necessidades de incorporação de tecnologias modernas e de sistema de tratamento dos efluentes, propiciando de maneira sustentável que haja a continuação do processo de produção do estaleiro e, ao mesmo tempo, permitindo o retorno da biodiversidade da Baía de Guanabara. / Guanabara Bay, located at Arsenal de Marinha do Rio de Janeiro (AMRJ), suffers from intense human pressure, especially on what is referred about water quality. In this context, a considerable part of the pollution comes from the shipyards activity, sine that the naval industrial activity in Rio de Janeiro has no mechanism for pollution control that matches its real potential polluter. This lack of inspection makes it possible the launch of solid waste in Guanabara Bay, in addition of sanitary sewage, chemical waste, oily and toxic, which increases the contamination of the water, shores and mangroves. These discard has been, more and more, a target of societys environmental demands and of laws also. Because of that, the management of wastewater from Arsenal has become a priority, for that, this study was suggested. Beginning with the analyses of the shipyard workshops, which dry docks activities have been identified as one of the most striking on the shipyard. From this conclusion, two research sources for impact reduction are presented. In the first stage there is a study of activities that creates wastewater in the dry dock of repair, with the purpose to propose the establishment of guidelines to improve the management practices, to minimize the production of liquid effluents and to help with the embracement of pro-active environmental practices. In the second research, with bases in world technologies, there is the purpose to treat the effluents from a dry dock, in which there were processes identified that would attend to the shipyards environmental needs, with options of choice between the partial treatment, for disposal in public network, or to the persecution of reuse procedure. The conclusions of this study point to the implantation of a systemized environmental management of the dry dock, rigorous and integrated with the management of vessels. Adding to it, the need to incorporate modern technologies and treatment systems of effluents, which will provide, in a sustainable way, that the process of production of the shipyard could proceed. And, at the same time, allowing the return of the biodiversity of the Guanabara Bay.

Orientador: Luis Paulo Barbour Scott. / Dissertação (mestrado) - Universidade Federal do ABC. Programa de Pós-Graduação em Engenharia da Informação.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2018-09-27T18:27:35Z No. of bitstreams: 1 2017 - Guilherme Santos Cabral de Oliveira.pdf: 2229116 bytes, checksum: 85b34776c7b3b4fd1ec2f5b644cd6d8e (MD5) / Made available in DSpace on 2018-09-27T18:27:35Z (GMT). No. of bitstreams: 1 2017 - Guilherme Santos Cabral de Oliveira.pdf: 2229116 bytes, checksum: 85b34776c7b3b4fd1ec2f5b644cd6d8e (MD5) Previous issue date: 2017-05-25 / This paper aims at understanding the dynamics established in the context, to what extent the experiences of dockworkers contributed to the acceptance of public policies related to the social security issue in Brazil in the 1930s and 1940s, Tracing as analysis of this process the years of operation of Institute of Retirement and Pensions of Stowage (IAPE), from its inception in 1938 until its incorporation to the Institute of Retirement and Pensions of Employees in Transport and Cargo (Iapetec) in 1945. The IAPE was considered by the State and the press as the "vanguard of Brazilian social security," sought to play the role of assistance in the labor context and aimed to be one of the catalysts of the corporatist project employed by the State in the 1930s and 1940s. However, in less than a decade had consummated its incorporation to Iapetec, after failing technical reforms. Nevertheless, the IAPE was an important tool for the use of Dockers to alleviate problems of their daily lives, of which we can highlight: insurance against sickness and accidents at work and birth assistance; Medium-surgical-hospital care. Absorbing some of the most urgent demands of these workers and their families. Nevertheless, we emphasize that the use of these public policies was also made possible by the strategic position in which the category was articulated in the midst of the national economic scenario, provoking an aspect of struggle for a workers' citizenship. Thus, we observe a confluence between interests of the Brazilian State in the proposed cut and the interests of dockworkers in the Federal Capital, revealing contradictions and more interpretations about the different conceptions of class. In order to do this, it was necessary to analyze the daily life of the workers in their work environments and places of sociability (sometimes both focus on the same space), to understand both the internal conflicts in which they were inserted and the variables that made them compose their class entity, the Union of Dock Workers. From this point on, we present different sources (interviews, periodicals, official documentation and minutes of the IAPE fiscal council), as the Brazilian State sought to undertake its social security policy and have to incorporate the demands of these workers. / O presente trabalho dedica-se a compreender diante das din?micas estabelecidas no contexto, em que medida as experi?ncias dos oper?rios estivadores contribu?ram para a acep??o de pol?ticas p?blicas relativas ? quest?o previdenci?ria no Brasil das d?cadas de 1930 e 1940, estabelecendo como fio condutor deste processo os anos de funcionamento do Instituto de Aposentadoria e Pens?es da Estiva (IAPE), desde de sua cria??o no ano de 1938 at? sua incorpora??o ao Instituto de Aposentadoria e Pens?es dos Empregados em Transportes e Cargas (Iapetec) no ano de 1945. O IAPE foi considerado pelo Estado e pela imprensa como a ?vanguarda do seguro social brasileiro?, procurou desempenhar o papel da assist?ncia no contexto trabalhista e objetivou ser um dos catalizadores do projeto corporativista empregado pelo Estado nos anos 30 e 40. No entanto, em menos de uma d?cada teve consumada sua incorpora??o ao Iapetec, ap?s malfadas reformas t?cnicas. N?o obstante, o IAPE constituiu uma importante ferramenta de utiliza??o dos trabalhadores estivadores para ameniza??o de problemas de seu cotidiano, dos quais podemos destacar: seguros contra doen?a e acidentes de trabalho e aux?lio natalidade; assist?ncia m?dia-cirurgica-hospitalar. Absorvendo algumas das demandas mais urgentes desses trabalhadores e suas fam?lias. Todavia, salientamos que o emprego dessas pol?ticas p?blicas foram possibilitadas tamb?m pela posi??o estrat?gica em que se articulava a categoria em meio ao cen?rio econ?mico nacional, suscitando um aspecto de luta por uma cidadania oper?ria. Dessa forma, observamos uma conflu?ncia entre interesses do Estado brasileiro no recorte proposto e os interesses dos trabalhadores estivadores da Capital Federal, revelando contradi??es e mais interpreta??es sobre as diferentes concep??es de classe. Para tal, nos fez necess?rio analisar o cotidiano dos trabalhadores estivadores em seus ambientes de trabalho e locais de sociabilidade (por vezes ambos se concentram no mesmo espa?o), para compreender tanto os conflitos internos nos quais estavam inseridos quanto as vari?veis que os faziam comp?r sua entidade de classe, a Uni?o dos Oper?rios Estivadores. A partir deste ponto apresentamos, convergindo fontes distintas (entrevistas, peri?dicos, documenta??o oficial e atas do conselho fiscal do IAPE), Como o Estado brasileiro buscou empreender sua pol?tica de previd?ncia social tendo de compreender as demandas dos trabalhadores.

This dissertation examines the livelihood strategies of African dock workers in Durban, South Africa, between the Anglo-Boer War and the 1959 strikes. These labourers did not conform to common conceptions of radical dock workers or conservative African migrant workers. While Marxist scholars have been correct to stress the working class consciousness of Durban’s dock workers, this consciousness was also more ambiguous. These workers and their leaders displayed a peculiar mix of concern for workers’ issues and defences of the rights and interests of African traders. Many of Durban’s dock workers were not only wage labourers. In fact, only a minority had wages as their only source of income. The Reserve economy played a role in sustaining the consumption levels of their households and, more importantly, more than half of the former dock workers interviewed for this research engaged in some form of commercial enterprise, often based on the pilferage and sale of cargoes. Some also teamed up with township women who sold pilfered goods while the men were at work. This combination of commercial strategies and wage labour has often been overlooked in the literature. By looking at these livelihood strategies, this dissertation considers how rural and urban economies interacted in households’ strategies and reinterprets the reproduction of labour and the household in order to move beyond dichotomies of proletarian versus rural consciousness. The dock workers’ households were neither proletarian households that were forced to reside in the countryside because of apartheid, nor traditional rural homesteads with a missing migrant member. The households were reproduced in three geographically separate spheres of production and consumption, none of which could reproduce the household on its own. These spheres were dependent on each other, but also separate, as physical distance gave the different household members some autonomy. Such multi-nodal households not only bridged the rural and the urban, but equally straddled the formal/informal divide. For many, their employment on the docks made their commercial enterprises possible, which allowed them to retire early from urban wage labour. Consequently, the interests of wage labourers could not be divorced from those of African small-scale entrepreneurs. / Thesis (Ph.D, History) -- Queen's University, 2011-09-26 17:14:17.474

A Baía de Guanabara, ambiente de localização do Arsenal de Marinha do Rio de Janeiro (AMRJ), sofre com a intensa pressão antrópica, principalmente no aspecto da qualidade das águas. Neste contexto, uma fração considerável da poluição decorre das atividades dos estaleiros, uma vez que a atividade industrial naval no Rio de Janeiro não tem mecanismo de controle de poluição à altura do seu real potencial poluidor. Esta ausência de fiscalização possibilita o lançamento na Baía de resíduos sólidos, esgotos sanitários, efluentes químicos, oleosos e tóxicos, tornando crescente a contaminação dessas águas, margens e mangues. Estes descartes cada vez mais são alvo das exigências ambientais da sociedade e das legislações. Devido a isto, a gestão de efluentes líquidos do Arsenal tornou-se prioritária, para tal, esse estudo foi proposto, tendo iniciado pela análise das oficinas do estaleiro, na qual as operações no dique foram identificadas como uma das mais impactantes do estaleiro. A partir desta constatação, estão apresentadas duas fontes de pesquisas para a redução dos impactos. Na primeira etapa, há o estudo das atividades geradoras de efluentes no dique de reparo, com os objetivos de propor a implantação das diretrizes de melhores práticas de gestão, de minimizar a geração de efluentes líquidos e de contribuir para a adoção de práticas ambientais proativas. Como segunda pesquisa, com base nas tecnologias mundiais, há a proposta de tratamento dos efluentes de um dique, na qual foram identificados os processos que irão atender às necessidades ambientais do estaleiro, com as opções de escolha entre o tratamento parcial, para o descarte na rede pública, ou com o prosseguimento do processo até o seu reúso. As conclusões deste estudo apontam para a implantação da gestão ambiental do dique sistematizada, rigorosa e integrada com a gestão das embarcações, acrescentando-se a isto, as necessidades de incorporação de tecnologias modernas e de sistema de tratamento dos efluentes, propiciando de maneira sustentável que haja a continuação do processo de produção do estaleiro e, ao mesmo tempo, permitindo o retorno da biodiversidade da Baía de Guanabara. / Guanabara Bay, located at Arsenal de Marinha do Rio de Janeiro (AMRJ), suffers from intense human pressure, especially on what is referred about water quality. In this context, a considerable part of the pollution comes from the shipyards activity, sine that the naval industrial activity in Rio de Janeiro has no mechanism for pollution control that matches its real potential polluter. This lack of inspection makes it possible the launch of solid waste in Guanabara Bay, in addition of sanitary sewage, chemical waste, oily and toxic, which increases the contamination of the water, shores and mangroves. These discard has been, more and more, a target of societys environmental demands and of laws also. Because of that, the management of wastewater from Arsenal has become a priority, for that, this study was suggested. Beginning with the analyses of the shipyard workshops, which dry docks activities have been identified as one of the most striking on the shipyard. From this conclusion, two research sources for impact reduction are presented. In the first stage there is a study of activities that creates wastewater in the dry dock of repair, with the purpose to propose the establishment of guidelines to improve the management practices, to minimize the production of liquid effluents and to help with the embracement of pro-active environmental practices. In the second research, with bases in world technologies, there is the purpose to treat the effluents from a dry dock, in which there were processes identified that would attend to the shipyards environmental needs, with options of choice between the partial treatment, for disposal in public network, or to the persecution of reuse procedure. The conclusions of this study point to the implantation of a systemized environmental management of the dry dock, rigorous and integrated with the management of vessels. Adding to it, the need to incorporate modern technologies and treatment systems of effluents, which will provide, in a sustainable way, that the process of production of the shipyard could proceed. And, at the same time, allowing the return of the biodiversity of the Guanabara Bay.

abstract: Cyanovirin-N (CVN) is a cyanobacterial lectin with potent anti-HIV activity, mediated by binding to the N-linked oligosaccharide moiety of the envelope protein gp120. CVN offers a scaffold to develop multivalent carbohydrate-binding proteins with tunable specificities and affinities. I present here biophysical calculations completed on a monomeric-stabilized mutant of cyanovirin-N, P51G-m4-CVN, in which domain A binding activity is abolished by four mutations; with comparisons made to CVN<super>mutDB</super>, in which domain B binding activity is abolished. Using Monte Carlo calculations and docking simulations, mutations in CVN<super>mutDB</super> were considered singularly, and the mutations E41A/G and T57A were found to impact the affinity towards dimannose the greatest. <super>15</super>N-labeled proteins were titrated with Man&#945;(1-2)Man&#945;, while following chemical shift perturbations in NMR spectra. The mutants, E41A/G and T57A, had a larger Kd than P51G-m4-CVN, matching the trends predicted by the calculations. We also observed that the N42A mutation affects the local fold of the binding pocket, thus removing all binding to dimannose. Characterization of the mutant N53S showed similar binding affinity to P51G-m4-CVN. Using biophysical calculations allows us to study future iterations of models to explore affinities and specificities. In order to further elucidate the role of multivalency, I report here a designed covalent dimer of CVN, Nested cyanovirin-N (Nested CVN), which has four binding sites. Nested CVN was found to have comparable binding affinity to gp120 and antiviral activity to wt CVN. These results demonstrate the ability to create a multivalent, covalent dimer that has comparable results to that of wt CVN. WW domains are small modules consisting of 32-40 amino acids that recognize proline-rich peptides and are found in many signaling pathways. We use WW domain sequences to explore protein folding by simulations using Zipping and Assembly Method. We identified five crucial contacts that enabled us to predict the folding of WW domain sequences based on those contacts. We then designed a folded WW domain peptide from an unfolded WW domain sequence by introducing native contacts at those critical positions. / Dissertation/Thesis / Doctoral Dissertation Biochemistry 2014

Here we present a novel Smartphone-based colorimeter and demonstrate its application to the measurements of glucose and protein concentrations in biological samples. The key innovation of our approach was to combine powerful image processing encoded into a mobile phone application with a low cost 3D printed sample holder that allowed to control lighting conditions and significantly improved sensitivity. Different solutions with protein and glucose concentrations ranging from 0 to 2000 mg/dL were prepared and analyzed using our system. The Smartphone-based colorimeter always correctly classified the corresponding reagent strip pads, what confirms that it can be used as a low cost alternative for commercial test strip analyzers.

This diploma thesis is devoted to the topic of riverside docks in Prague. Riverside dock is a coastal area, which is on a lower level than an embankment. Previously used as docks for cargo and passenger ships and storage of sand. Nowadays they fulfill mainly recreational and socio-cultural function. The case study is focused on one of Prague's riverside docks, the riverside dock on Rašínovo nabrezi. In this thesis, I will focus on the description and explanation of the use of the riverside dock on Rašínovo nábřeží. Concentrating on its practical operation, conflicts of interests of individual participants and the impact on the environment and on the direct surroundings.

Protein-protein interactions build large networks which are essential in understanding complex diseases. Due to limitations of experimental methodology there are problems with large amounts of false negative and positive interactions; and a large gap in the amount of known interactions and structurally determined interactions. By using computational methods these problems can be alleviated. In this thesis the quality of a newly developed pipeline (InterPred) were investigated for its ability to generate coarse interaction models and score them. This ability was investigated by performing docking experiments in Rosetta on models generated in InterPred. The results suggest that InterPred is highly successful in generating good starting points for docking proteins in silico and to distinguish the quality of models.

No description available.