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The genus dombeya (sterculiaceae) in continental Africa : based on an unpublished doctoral thesis presented at Oxford university and supervised by F. White /Seyani, J. H. White, Frank, Bamps, Paul. January 1991 (has links)
Texte remanié de: Doctoral thesis--Philosophy--Oxford university, 1982. / Bibliogr. p. 175-184.
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Identification and characterisation of compounds with antimycobacterial activity from the leaves of Dombeya rotundifolia HochsMashilo, Matsilane Lethabo January 2023 (has links)
Thesis (M.Sc. (Microbiology)) -- University of Limpopo, 2023 / Plants are not only an important source of medicines, but also play a significant role
in drug development for the treatment of diseases such as Tuberculosis (TB). TB is a
pulmonary disease that is caused by Mycobacterium tuberculosis complex. The aim
of the study is to identify and characterise antimycobacterial compounds from
Dombeya rotundifolia. The plant was collected from the University of Limpopo, dried
and ground into fine powder. Extraction was done using different solvents that differ
in polarity. The plant was screened and analysed for phytochemicals. Three major
phytochemicals were quantified using reagent assays and analysed using standard
curves. The antioxidant activity of the plant was determined using 2,2-Diphenyl-1-
picrylhydrazyl (DPPH) and ferric reducing power assay. The antibacterial activity of
the plant extracts was tested against Mycobacterium smegmatis using bioautography
and serial microplate broth dilution assay. The antibiofilm activity of the plant extracts
were evaluated using crystal violet assay. The anti-inflammatory activity of the plant
was determined using egg albumin protein denaturation assay. The cytotoxic effects
of the extracts were evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl
tetrazolium bromide (MTT) assay on THP-1 cell line. The antimycobacterial
compounds were isolated and purified using bioassay-guided assay incorporated with
column chromatography and preparative TLC. The isolated compounds were
analysed and identified using nuclear magnetic resonance spectroscopy (NMR). The
results obtained in this study showed that water was the best extractant, extracting
57.6 mg of the plant material, followed by methanol with 38.1 mg and hexane as the
least extractant with 12.4 mg. In the phytochemical analysis of TLC plates, there was
a separation of compounds in all the mobile systems, and a clear separation of
compounds was observed in the BEA mobile system, followed by the EMW system.
The plant has all major phytochemicals that are needed for drug development. The
plant extracts had antioxidant activity, which was confirmed by the quantitative assays.
The high inhibitory activity of the extracts was indicated by low MIC values that
inhibited the bacterial growth. The butanol extract had the lowest MIC value (0.13
mg/mL). The plant extracts were able to prevent the formation of biofilm at different
concentrations. The plant was proven to have anti-inflammatory properties by having
a high inhibition capacity to prevent protein denaturation. The cytotoxicity results
showed that the plant was toxic to the cells. The isolated antimycobacterial
compounds were identified as Eicosanoic acid and Docosanoic. They have moderate
activity with the MIC value of 0.25 mg/mL. The study indicated that the isolated
compounds have antimycobacterial activity, which showed that the plant has a
potential to treat TB or symptoms related to TB. However, further studies are needed
to evaluate their toxic effects before use. / The National Research Foundation (NRF)
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Ecologie et évolution de la dioécie et du dimorphisme sexuel de la taille des fleurs chez les "dombeya"(sterculiacées) endémiques de La RéunionHumeau, Laurence 09 February 1999 (has links) (PDF)
Les forts taux d'espèces dioïques sur les îles ont longtemps fasciné les biologistes. Or le fonctionnement de la dioécie en milieu insulaire a été peu abordé. Les variations dans l'expression de la dioécie et du dimorphisme sexuel de la taille des fleurs sont aujourd'hui examinés dans l'archipel des Mascareignes. A la réunion, les espèces unisexuées du genre "Dombeya" (sterculiacées) sont dioïques cryptiques mais présentent une variabilité dans l'expression de la dioécie. Certaines espèces strictement dioïques constituent de grandes populations en altitude alors que les espèces partiellement dioïques forment de petites populations à basse altitude. Par ailleurs, l'hybridation entre espèces strictement dioïques et partiellement dioïques semble engendrer des taxons hermaphrodites autocompatibles. Ces résultats demandent confirmation par une étude moléculaire plus poussée, mais l'hybridation en milieu insulaire semble être un processus évolutif important dans ce genre. Nos résultats montrent que le dimorphisme sexuel de la taille des fleurs de la flore unisexuée indigène de la Réunion est un phénomène largement répandu. Le biais observé est souvent en faveur des individus mâles, ce qui peut être corrélé à la zoogamie, fréquente sur cette île. Le dimorphisme est plus abondant chez les espèces endémiques que chez les taxons indigènes et est variable entre les espèces apparentées. Un modèle allométrique est proposé pour illustrer ces variations au sein du genre "Dombeya". Cette thèse apporte les connaissances de base sur la biologie du genre "Dombeya" à la Réunion dont certaines espèces constituent les éléments clés des forêts primaires et d'autres sont des espèces rares des milieux fragmentés.
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