Extending the use of Doppler spectroscopy as an important plasma diagnostic -- by developing a multi-channel system capable of tomography -- is the foundation of this thesis. A system which can simultaneously measure the emissivity, temperature and flow velocity of plasma ions has been installed, calibrated and operated on the H-1NF heliac, yielding comprehensive and interesting results. The measurements are time-resolved, made from a large range of viewing positions and, using scalar tomographic inversion methods, can be unfolded to give two-dimensional images of ion emissivity and temperature. The flow velocity profiles, while not inverted, nevertheless lead to a greater understanding of the plasma behaviour.¶ Fifty-five lens-coupled optical fibres, mounted on a large rotatable stainless steel ring, encircle the plasma poloidally and transport light to a multi-channel Fourier-transform spectrometer. This `coherence-imaging' spectrometer employs an electro-optically modulated birefringent crystal plate to monitor the coherence of an isolated spectral line. Measurement of the intensity, fringe visibility and phase of the resulting interferogram leads to values for the emissivity, ion temperature and flow velocity. Using a multi-anode photomultiplier assembly, allows the time-resolved detection of all optical channels simultaneously.¶ The system has been fully calibrated, including a measurement of the spatial response of each line-of-sight. The calibration procedure accounts for the relative channel sensitivities, the response of the line filter and the removal of detector cross-talk. In situ light sources are installed provide routine and accurate relative intensity calibration of the system.¶ Methods of tomography provide the unfolding of the measured plasma parameters to construct two-dimensional images of ion temperature and emissivity. Methods of inversion include the iterative ART routine -- using projection data gathered with the light-collecting optics rotated to different viewing positions -- and linear composition of Fourier-Bessel basis functions -- with the data obtained from a single unrotated viewing position. ART reconstructions of the emissivity are performed without the need for a priori information while those of the ion temperature are computed using regularising functions to help stabilise the inversion.¶ This new system -- named ToMOSS for Tomographic Modulated Optical Solid-state Spectrometer -- enables a more detailed study of various plasma phenomena observed in H-1NF. Among other results, this thesis presents the first tomographic reconstructions of emissivity and temperature fluctuations associated with a large-scale coherent instability.

Cette thèse présente l'étude de l'interaction de lumière cohérente avec une couche sub-longueur d'onde de vapeur alcaline atomique confinée en nano-cellule et applications pour la formation de résonances optiques étroites.Nous développons un modèle théorique décrivant l'interaction résonante de lumière laser avec la couche mince de vapeur alcaline en présence d'un champ magnétique. Nous montrons qu'en raison d'un régime transitoire d'interaction, seuls les atomes lents contribuent au signal et leur spectre de transmission est essentiellement sans effet Doppler. La nature des spectres obtenus fait de la spectroscopie de transmission, en nano-cellule, une technique pratique pour l'étude de transitions très rapprochées et l'évolution de leur comportement dans un champ magnétique. Des expériences réalisées pour des champs magnétiques jusqu'à 7000 G montrent un excellent accord entre théorie et expérience.Nous explorons aussi la rotation Faraday du plan de polarisation de la lumière lors de sa propagation dans la couche mince atomique. Bien que l'angle de rotation soit très faible, nous observons que les résonances des spectres de rotation Faraday sont plus étroites que celles de transmission. Enfin, nous étudions de nouvelles possibilités pour former des résonances optiques étroites et montrons qu'un traitement par deuxième dérivée des spectres de transmission donne le meilleur rétrécissement de raies parmi toutes les méthodes étudiées dans cette thèse. / This thesis presents the study of coherent light interaction with a sub-wavelength atomic alkali vapor layer confined in a nano-cell and applications for the formation of narrow optical resonances.We develop a theoretical model describing the resonant interaction of the laser light with the thin alkali vapor layer in the presence of an external static magnetic field. We show that due to a transient regime of interaction, only slow atoms contribute to the signal and their transmission spectrum is essentially Doppler-free. The nature of the obtained spectra makes the transmission spectroscopy from a nano-cell a convenient technique to perform studies of closely-spaced atomic transitions and investigate their behavior in magnetic fields. Experimental realizations for magnetic field up to 7000~G show an excellent agreement between theory and experiment.We also explore the Faraday rotation of the plane polarization of light with the propagation through the thin atomic slab. We see that despite a small angle of rotation, Faraday rotation spectra exhibit resonances narrower than that for transmission. At last, we investigate new possibilities to form narrow optical resonances in nano-cells and show that second derivation processing of transmission spectra yields the strongest line narrowing among all methods studied in this thesis.

Noise-immune cavity-enhanced optical heterodyne molecular spectro-metry (NICE-OHMS) is one of the most sensitive laser-based absorption techniques. The high sensitivity of NICE-OHMS is obtained by a unique combination of cavity enhancement (for increased interaction length with a sample) with frequency modulation spectrometry (for reduction of noise). Moreover, sub-Doppler detection is possible due to the presence of high intensity counter-propagating waves inside an external resonator, which provides an excellent spectral selectivity. The high sensitivity and selectivity make NICE-OHMS particularly suitable for trace gas detection. Despite this, the technique has so far not been often used for practical applications due to its technical complexity, originating primarily from the requirement of an active stabilization of the laser frequency to a cavity mode. The main aim of the work presented in this thesis has been to develop a simpler and more robust NICE-OHMS instrumentation without compro-mising the high sensitivity and selectivity of the technique. A compact NICE-OHMS setup based on a fiber laser and a fiber-coupled electro-optic modulator has been constructed. The main advantage of the fiber laser is its narrow free-running linewidth, which significantly simplifies the frequency stabilization procedure. It has been demonstrated, using acetylene and carbon dioxide as pilot species, that the system is capable of detecting relative absorption down to 3 × 10-9 on a Doppler-broadened transition, and sub-Doppler optical phase shift down to 1.6 × 10-10, the latter corresponding to a detection limit of 1 × 10-12 atm of C2H2. Moreover, the potential of dual frequency modulation dispersion spectrometry (DFM-DS), an integral part of NICE-OHMS, for concentration measurements has been assessed. This thesis contributes also to the theoretical description of Doppler-broadened and sub-Doppler NICE-OHMS signals, as well as DFM-DS signals. It has been shown that the concentration of an analyte can be deduced from a Doppler-broadened NICE-OHMS signal detected at an arbitrary and unknown detection phase, provided that a fit of the theoretical lineshape to the experimental data is performed. The influence of optical saturation on Doppler-broadened NICE-OHMS signals has been described theoretically and demonstrated experimentally. In particular, it has been shown that the Doppler-broadened dispersion signal is unaffected by optical saturation in the Doppler limit. An expression for the sub-Doppler optical phase shift, valid for high degrees of saturation, has been derived and verified experimentally up to degrees of saturation of 100.

<div>Biological dynamics have been studied by many methods. Fluorescence dynamic microscopy and optical coherence tomography provided fundamental understandings of biological systems. However, their high NA optics only represent local characteristics. Biodynamic imaging (BDI) technique implements a low NA optics and acquires the statistical average of Doppler shifts that occurred by dynamic light scattering with biological dynamic subsystems provided globally averaged dynamic characteristics. </div><div>BDI is used for this study to investigate biomedical applications. The chemotherapy efficacy measurement by BDI demonstrated a good agreement between the Doppler spectral phenotypes and the preclinical outcomes. Also, dynamic responses of microbiomes by chemical stimuli demonstrated featured Doppler characteristics. The bacterial infection of epithelial spheroids showed consistent spectral responses and antibiotic-resistant E. coli infection treatment with a sensitive and resistive antibiotic showed a dramatic contrast. Furthermore, the phase-sensitive characteristics of BDI provided a clue to understanding the characteristics of the random process of biological systems. Levy-like heavy-tailed probability density functions are demonstrated and </div><div>the shape changed by infection will be discussed. </div>

Background: Topical negative pressure wound therapy (TNPWT) is a regularly used method in modern wound treatment with a growing and diverse potential for clinical use. So far positive effects on microcirculation have been observed and examined, although precise statements on the underlying mechanism appear unsatisfying. Objective: The aim of our study was to extend the understanding of the effect of TNPWT on tissue perfusion and determine the time frame and the extent to which the tissue perfusion changes due to TNPWT. Material and methods: TNPWT was applied to the anterior thighs of 40 healthy individuals for 30 min, respectively. Before and up to 90 min after the application, measurements of the amount of regional haemoglobin (rHb), capillary venous oxygen saturation (sO2), blood flow (flow) and velocity were conducted with spectrophotometry (combining white light spectrometry and laser Doppler spectroscopy) within two different depths/skin layers. A superficial measuring probe for depths up to 3 mm and a deep measuring probe for up to 7 mm were used. Results: All parameters show significant changes after the intervention compared to baseline measurements. The greater effect was seen superficially. The superficially measured rHb, sO2 and flow showed a significant increase and stayed above the baseline at the end of the protocol. Whereas deeply measured, the rHb initially showed a decrease. The flow and sO2 showed a significant increase up to 60 min after the intervention. Conclusion: The application of TNPWT on healthy tissue shows an increase in capillary-venous oxygen saturation and haemoglobin concentration of at least 90 min after intervention. A possible use in clinical practice for preconditioning to enhance wound healing for high-risk patients to develop wound healing disorder, requires further studies to investigate the actual duration of the effect.