Spelling suggestions: "subject:"dragline milk"" "subject:"dragline silk""
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Biomimetic Polymer Systems via RAFT Polymerization - Routes to High-Performance MaterialsHendrich, Michael 02 December 2016 (has links)
No description available.
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Characterization of a New Family of Cysteine Rich Proteins in Black Widow Spider SilkWilliams, Caroline 01 January 2016 (has links)
Spiders are capable of producing a variety of silk types, each with their own unique protein composition and function. Dragline silk in particular, has been of great interest due to its high tensile strength and extensibility. In the past, synthetically produced dragline fibers have not been able to match the superior properties of natural silk. A recent discovery in the western black widow spider, Latrodectus hesperus, might be the missing link between the current state of synthetic silks and naturally produced fibers. Our research is centered around the discovery a new family of five low-molecular-weight cysteine-rich proteins (CRPs) and their potential function within dragline silk. This study focuses on the characterization of recombinantly expressed CRP1, CRP2, and CRP4. Through structural analysis using circular dichroism, it has been determined that the CRP family members have mostly alpha-helical secondary structure and exhibit small differences in their ability to maintain their structure in the presence of changing environmental conditions. The study also covers the effects of temperature and pH on the folding and unfolding of the CRPs. It appears that pH is the dominant influence on protein unfolding within the major ampullate gland.
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Study of Physical Protein-Protein Interactions Between the MaSp1 C-Terminal Domain and Small Cysteine-Rich Proteins Found in the Major Ampullate Gland of Latrodectus hesperusRabara, Taylor Renee 01 January 2016 (has links)
Spiders spin a wide variety of different silk types with different biological functions that are known for their extraordinary mechanical properties. Dragline silk has predominantly captured the interest of researchers because it exhibits high tensile strength and toughness while maintaining its elasticity. This thesis has focused on the characterization of a family of small molecular weight proteins recently discovered in dragline silk. These proteins were discovered in the western black widow spider, Latrodectus hesperus, and have been termed Cysteine-Rich Proteins (CRPs) due to their high conserved cysteine content. CRP family members were used in protein-protein interaction studies to determine if there is any interaction with the major ampullate spidroins (MaSps). After affinity chromatography and co-expression studies in bacteria, there were no detectable interactions between the CRPs and MaSp1. Further studies
which could be an important role in the natural silk assembly process. Further protein interaction studies in different salt and pH conditions can further determine the function of the CRPs in dragline silk formation.
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Does Larinoides cornutus major ampullate silk have shape memory property?Su, Yuhan 28 April 2021 (has links)
No description available.
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