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Vasa function in Drosophila pole plasmLiang, Lu January 1996 (has links)
No description available.
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The role of vasa during oogenesis /Styhler, Sylvia. January 1998 (has links)
No description available.
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The molecular basis of the tumorous head phenotype in drosophila melanogasterRamoth, Lance L. 01 April 2001 (has links)
No description available.
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Genetic analysis of the gene Additional sex combs and interacting lociNicholls, Felicity K. M. January 1990 (has links)
In order to recover new mutant alleles of the Polycomb group gene Additional sex combs (Asx), mutagenized chromosomes were screened over the putative Asx allele XT129. Thirteen new mutant strains that fail to complement XT129 were recovered. Unexpectedly, the thirteen strains sorted into four complementation groups. Recombination mapping suggests that each complementation group represents a separate locus. The largest group fails to complement a deletion of Asx and maps in the vicinity of 2-72, the published location of Asx. All new mutant strains enhance the phenotype of Polycomb mutant flies and are not allelic to any previously discovered second chromosome Polycomb group genes. Therefore, the new mutants may be considered putative new members of the Polycomb group. This study suggests that Asx belongs to a sub-group of genes displaying intergenic non-complementation. / Science, Faculty of / Zoology, Department of / Graduate
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Isolation and characterization of a cyclin-dependent kinase-activating kinase in Drosophila melanogasterLarochelle, Stéphane. January 1998 (has links)
Protein phosphorylation is now recognized to be one of the most important means of regulating protein activity. An approach was taken that was aimed at identifying new protein Serine/Threonine kinase genes in the fruit fly Drosophila melanogaster. Three of the kinases identified were chosen for molecular characterization: a Map kinase-activated protein kinase-2 homolog (DmMAPKAPK-2); a novel female germline specific kinase ( loki); and the homolog of the vertebrate cdk7 genes (Dmcdk7). Among those, Dmcdk7 was chosen for in depth molecular and genetic characterization. Cdk7 has previously been shown in vertebrate systems to phosphorylate and activate many different Cyclin-dependent kinases (Cdks) in vitro. However, conclusive evidence that Cdk7 could act as a Cdk-activating kinase (CAK) in vivo had remained elusive, and became even controversial. Adding to the controversy was the fact that in the budding yeast S. cerevisiae, CAK activity is provided by the CAK1/Civ1 protein which is unrelated to Cdk7. It was therefore proposed that the CAK activity of Cdk7 may be an in vitro artefact. In an attempt to resolve this issue null and temperature sensitive mutations of the Dmcdk7 gene have been created. The results obtained using these mutant alleles of Dmcdk7 demonstrate that cdk7 is necessary for CAK activity in vivo in a multicellular organism. It is shown that cdk7 activity is required for the activation of both Cdc2/Cyclin A and Cdc2/Cyclin B complexes, and for cell division. In addition to validate the function of Cdk7 as a bona fide regulator of the cell cycle, these results suggest that there may be a fundamental difference in the way metazoans and budding yeast effect a key modification of Cdks. Phosphorylation events at different sites (including the T-loop) are also known to be involved in stabilizing the Cdk7/Cyclin H dimer in vitro, and have been shown to occur in vivo. Surprisingly, the in vivo analysis of different phosphorylation mutant forms of DmCdk7 f
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Isolation and characterization of a cyclin-dependent kinase-activating kinase in Drosophila melanogasterLarochelle, Stéphane. January 1998 (has links)
No description available.
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Evolutionary and functional relationships of insect immune proteins / Marco Fabrri.Fabbri, Marco January 2003 (has links)
"May 2003" / Bibliography: leaves 72-87. / iii, 87 leaves : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Applied and Molecular Ecology, 2003
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Regulation by male-specific fruitless of neural circuitry used during courtship and copulation behavior in Drosophila melanogasterLatham, Kristin Lynn 09 June 2005 (has links)
Courtship and copulation behaviors in Drosophila melanogaster males are
regulated by sex-specific products from the gene fruitless (fru). Male-specific FRU
proteins (FRU[superscript M]) are putative transcription factors of the BTB-ZnF family that likely
act by controlling development and maintenance of the neural circuitry used during
male sexual behavior. However, which neuronal characteristics are regulated by
FRU[superscript M] is mostly unknown and how FRU[superscript M] neurons are grouped into circuits and the
role that specific neuronal circuits play in sexual behavior has not been elucidated. I
have identified a subset of FRU[superscript M] neurons that co-express the transcription factor,
Engrailed (En). After fru[superscript M]-RNAi-induced targeted removal of FRU[superscript M] proteins from
FRU[superscript M]/En neurons, males were impaired in their ability to initiate or maintain
copulation. Further, I examined two characteristics, the initial projections and
neurotransmitters used by FRU[superscript M]/En neurons. Males and females showed a difference
in the neurochemistry of FRU[superscript M]/En neurons in the thoracic ganglia; this
neurochemistry is disrupted in fru mutant males.
For one cohort of serotonergic neurons in the abdominal ganglion that were
previously shown to be dependent on FRU[superscript M] for expression of serotonin, I determined
that FRU[superscript M] works in conjunction with other sex-specific genes, TAKEOUT (TO) and
DOUBLESEX (DSX), to induce of serotonin expression in males; in females
serotonin expression is repressed by DSX and TO.
Finally, I performed a genetic screen for genes that interact with, or are
downstream targets of, fru, dsx, or dissatisfaction (dsf). I assessed fertility, copulation
success, and abdominal muscle development of EMS-mutagenized flies, resulting in
one fly line in which homozygous mutant animals had a novel muscle phenotype. By
genetic tests, the mutation was found to be allelic to string, which encodes a Cdc25-
like phosphatase.
Taken together, my research demonstrates that subsets of FRU[superscript M] neurons
function in circumscribed circuits to regulate specific portions of sexual behavior, and
that FRU[superscript M], along with other sex-specific genes, controls development of these
neurons in part by determining neurochemistry. Further, FRU[superscript M] likely directs multiple
downstream targets, in different subsets of neurons in which it is expressed, which
collectively provide correct development of neural circuits underlying courtship and
copulation behavior. / Graduation date: 2006
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The genetic dissection of the fruitless gene's functions during embryogenesis in Drosophila melanogasterSong, Ho-Juhn 16 August 2001 (has links)
The fruitless (fru) gene in Drosophila melanogaster is a multifunctional
gene having sex-specific functions in the regulation of male sexual behavior and
sex-nonspecific functions affecting adult viability and external morphology. While
much attention has focused on fru's sex-specific roles, little is known about its sex-nonspecific
functions. The embryonic central nervous system (CNS) is a prime
model system in which to study the genetic control of axonal outgrowth and proper
CNS formation. I have examined fru's sex-nonspecific role in embryonic neural
development. fru transcripts and FRU proteins from sex-nonspecific promoters are
expressed beginning at the earliest stages of neurogenesis and subsequently in both
neurons and glia. In embryos that lack most or all fru function, Fasciclin II- and
BP102-positive axons appeared to defasciculate from their normal pathway and
fasciculate along aberrant neuronal pathways, suggesting that one of fru's sex-nonspecific
roles is to regulate axonal differentiation. I next examined whether the
loss of fru function in FRU-expressing neuronal precursors causes neuronal fate
change. Analysis of fru mutant embryos revealed a lack of Even-skipped (Eve)
staining in Eve-expressing neurons, ectopic Eve staining in non-Eve-expressing
neurons and mispositioned dorsal Eve-expressing neurons, which suggests that fru
functions to maintain neuronal identity rather than to specify neuronal fate. In fru
mutants these defects in axonal projections and in Eve staining were rescued by the
expression of specific fru transgenes.
To better understand fru's function in the formation of the embryonic CNS,
I dissected out fru's function in neuron and glia through a genetic interaction study.
fru genetically interacts in neurons with longitudinal lacking to make proper axonal
projections. In addition, fru might be in the same genetic pathway as roundabout
(robo), a repulsive guidance receptor, and commissureless, a downregulator of
Robo, to ensure proper axonal pathfinding. Surprisingly, fru interacts with
tramtrack and glial cells missing to repress neuronal differentiation in the lateral
glia and with single-minded for the development of midline glia. Taken together,
fru function is required for proper axonal pathfinding in neurons and for proper
development of lateral and midline glia. / Graduation date: 2002
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Identification of Prospero targets during neurogenesis of Drosophila melanogasterChoksi, Semil P. January 2006 (has links)
No description available.
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