Two populations derived by crossing LDN x LDN Dic-3A (Population I) and LDN x LDN Dic-3B (Population II) were genetically characterized for the seed dormancy present on chromosome 3A and 3B of durum wheat. The genes for seed dormancy in these two populations were contributed by the wild parent T. dicoccoides. Although the populations showed transgressive segregants for both dormant as well as nondormant parent, the populations were similar to the dormant parent at Langdon and Prosper 2006 field locations for Population I and at Langdon 2007 and Autumn greenhouse season for Population II. Genotypic and phenotypic analysis over the combined populations showed an environmental effect on expression of the trait. Different QTL were identified for both field and greenhouse season for the population derived from the cross between LDN x LDN Dic-3A. Five QTL for seed dormancy were identified on chromosome 3A for the QTL analysis performed over combined field locations. One QTL ranging between marker interval Xcfa2193 and Xcfd2a was consistently present for the 30 day period of seed germination and was also found to be linked to red grain color trait. The QTL analysis performed on the population derived from the cross between LDN x LDN Dic-3B identified only one major QTL on the long arm of chromosome 3B between the marker interval Xbarc84 and Xwmc291. This QTL was consistently present for all the field and spring greenhouse season for the seed germination period of 30 days. The QTL x E effect was also observed for this QTL, however it was very small.

Association mapping (AM), based on linkage disequilibrium, is a complementary strategy to traditional quantitative trait loci (QTL) mapping for describing associations between genotypes and phenotypes in crop plants. Yellow endosperm colour, an important quality trait in durum wheat (<i>Triticum turgidum L. var. durum</i>), was studied to determine the potential of AM to (1) identify previously reported QTL using a genome wide scan and (2) to determine allelic association of the phytoene synthase 1 (Psy1) gene using a candidate gene analysis. At present, a number of QTL for endosperm colour have been identified, and phytoene synthase, the initial enzyme of the carotenoid biosynthetic pathway, has been associated with QTL on the group 7 chromosomes which are considered to play a significant role in expression of yellow pigment concentration. CIE 1976 b*, a light reflectance measurement, and water-saturated butanol extracted pigments were assessed on a collection of 93 elite accessions from a variety of geographic origins, and genotyped with 245 markers. Population structure was assessed using genetic distance and Bayesian model based approaches, identifying five sub-populations consistent with breeding origin and pedigree. Association analysis identified significant associations with yellow endosperm colour on all chromosomes, including several previously identified QTL as well as new regions for genomic dissection. Pairwise LD mapping of Psy1-B1 and Psy1-A1 located the genes to chromosomes 7B and 7A respectively, to regions which have previously been identified for yellow pigment concentration QTL. The results of this study indicate that AM can be used to complement traditional QTL mapping techniques, and identify novel QTL for further study.

Yellow pigment (YP) concentration is an important quality trait in durum wheat (<i>Triticum turgidum</i> L. var <i>durum</i>) and is comprised primarily of carotenoids. The main objective of our study was to measure the accumulation of carotenoids during the grain fill period to improve our understanding of the physiological basis for differences among durum wheat cultivars. Thirteen cultivars and breeding genotypes with large variation in total YP concentration (<6 µg g-1 to >15 µg g-1) were studied. Spikes were sampled from replicated field plots in 2007 and 2008 near Saskatoon and Swift Current, Saskatchewan, Canada, at 14, 21, 28 and 35 days after heading (DAH). The remainder of each plot was combined at grain maturity for YP and carotenoid analysis. Carotenoids were extracted with 1:1 methanol:dichloromethane (0.1% BHT) and quantified with HPLC. <i>Trans</i> (E)-lutein was the predominant carotenoid at maturity and was detected at 14 DAH in all genotypes. The rate and duration of E-lutein accumulation was variable among genotypes expressing high, intermediate and low YP. The accumulation of all carotenoids was lowest in genotypes expressing low YP, and suggests rate limitations early in the carotenoid biosynthetic pathway. E-zeaxanthin concentrations were highest in mature grain, but no significant differences were detected among genotypes. However, the ratio of E-zeaxanthin to E-lutein was inversely correlated with total YP, suggesting that the â,å branch of lycopene cyclization is favoured over the â,â branch in high-YP genotypes. These results provide insights to the regulation of the carotenoid biosynthetic pathway during grain fill stage in durum wheat and will facilitate breeding for higher carotenoid concentration.

Association mapping (AM), based on linkage disequilibrium, is a complementary strategy to traditional quantitative trait loci (QTL) mapping for describing associations between genotypes and phenotypes in crop plants. Yellow endosperm colour, an important quality trait in durum wheat (<i>Triticum turgidum L. var. durum</i>), was studied to determine the potential of AM to (1) identify previously reported QTL using a genome wide scan and (2) to determine allelic association of the phytoene synthase 1 (Psy1) gene using a candidate gene analysis. At present, a number of QTL for endosperm colour have been identified, and phytoene synthase, the initial enzyme of the carotenoid biosynthetic pathway, has been associated with QTL on the group 7 chromosomes which are considered to play a significant role in expression of yellow pigment concentration. CIE 1976 b*, a light reflectance measurement, and water-saturated butanol extracted pigments were assessed on a collection of 93 elite accessions from a variety of geographic origins, and genotyped with 245 markers. Population structure was assessed using genetic distance and Bayesian model based approaches, identifying five sub-populations consistent with breeding origin and pedigree. Association analysis identified significant associations with yellow endosperm colour on all chromosomes, including several previously identified QTL as well as new regions for genomic dissection. Pairwise LD mapping of Psy1-B1 and Psy1-A1 located the genes to chromosomes 7B and 7A respectively, to regions which have previously been identified for yellow pigment concentration QTL. The results of this study indicate that AM can be used to complement traditional QTL mapping techniques, and identify novel QTL for further study.

Yellow pigment (YP) concentration is an important quality trait in durum wheat (<i>Triticum turgidum</i> L. var <i>durum</i>) and is comprised primarily of carotenoids. The main objective of our study was to measure the accumulation of carotenoids during the grain fill period to improve our understanding of the physiological basis for differences among durum wheat cultivars. Thirteen cultivars and breeding genotypes with large variation in total YP concentration (<6 µg g-1 to >15 µg g-1) were studied. Spikes were sampled from replicated field plots in 2007 and 2008 near Saskatoon and Swift Current, Saskatchewan, Canada, at 14, 21, 28 and 35 days after heading (DAH). The remainder of each plot was combined at grain maturity for YP and carotenoid analysis. Carotenoids were extracted with 1:1 methanol:dichloromethane (0.1% BHT) and quantified with HPLC. <i>Trans</i> (E)-lutein was the predominant carotenoid at maturity and was detected at 14 DAH in all genotypes. The rate and duration of E-lutein accumulation was variable among genotypes expressing high, intermediate and low YP. The accumulation of all carotenoids was lowest in genotypes expressing low YP, and suggests rate limitations early in the carotenoid biosynthetic pathway. E-zeaxanthin concentrations were highest in mature grain, but no significant differences were detected among genotypes. However, the ratio of E-zeaxanthin to E-lutein was inversely correlated with total YP, suggesting that the â,å branch of lycopene cyclization is favoured over the â,â branch in high-YP genotypes. These results provide insights to the regulation of the carotenoid biosynthetic pathway during grain fill stage in durum wheat and will facilitate breeding for higher carotenoid concentration.

Durum wheat (Triticum durum Desf.) is produced primarily in the Northern Great Plains and the Pacific Southwest of the United States. Current germplasm is predominantly of the spring growth habit. The objective of this study was to determine the feasibility of winter durum production in Virginia based upon both yield and quality parameters. Adaptation and yield potential of available winter durum lines were assessed from 1993-1998, in three physiographic regions in Virginia. The highest average durum yields were produced in the northern piedmont plateau at the Orange County location. Winter durum yields generally averaged 1600-2800 kg/ha less than soft red winter wheat, traditionally grown in the state. The price premium for high quality durum can compensate for this difference in yield. Based on average durum yields, and assuming the grain meets U.S. No. 2 Hard Amber Durum standards, durum production in Virginia would have been more profitable than soft red winter wheat production in 1994 and 1997. Physical and chemical quality analyses of the top 19 performing durum lines were performed to determine grain marketability, suitability for pasta, and potential consumer acceptance of the end product. Protein content and gluten strength of the Virginia grown durum were acceptable. Color, firmness, and cooking loss of pasta produced from Virginia grown durum were comparable to pasta produced from commercial semolina. Requirements for U.S. No. 2 Amber Durum were met by 21% of the lines in both 1996 and 1997. Overall, the wet, humid Virginia climate was the greatest hindrance to durum production and quality. The field trials and quality analyses showed that high quality durum production in Virginia is possible, but not consistent over all years. / Master of Science

Bibliography: leaves 148-160. A series of experiments was conducted to examine the growth and nutrient uptake of durum and bread wheat at a number of sites in South Australia. The experiments examined response to water stress, the pattern of root and shoot growth, soil water extraction and nutrient uptake among a range of adapted bread wheat and durum wheat cultivars.

Title page, table of contents and abstract only. The complete thesis in print form is available from the University of Adelaide Library. / Transient salinity in soils is characterised by high concentrations of salts in the subsoil. Durum wheat (Triticum turgidum L. Var. durum) is less tolerant of transient salinity than locally developed bread wheat (Triticum aestivum) varieties, and this results in reliable durum production being restricted to relatively unaffected soils. Field trials were conducted to assess the relative impact of transient salinity, boron toxicity and bicarbonate on crop production and highlighted the importance of combining tolerance to all three subsoil constraints into varieties intended for widespread adoption; and if the area of durum production is to be expanded. The Na exclusion locus from the landrace Na49 was found to improve the adaptation of durum to sites affected by transient salinity and is now being intogressed into a wide range of breeding material. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1152134 / Thesis (Ph.D.) -- University of Adelaide, School of Agriculture and Wine, 2005

Genetic resistance to the orange wheat blossom midge (Sitodiplosis mosellana; OWBM) is an important breeding target to prevent yield and quality losses of durum wheat produced in western Canada. To date, only a single characterized midge resistance gene, Sm1, has been identified. Sm1 confers antibiosis resistance to the OWBM. It has been genetically localized to chromosome 2BS of hexaploid wheat (Triticum aestivum L.). Sm1 has been introgressed into locally adapted germplasm. Currently, no Sm1 carrying durum wheat lines are available for commercial production, and no studies have characterized the influence of Sm1 on yield and end-use quality of durum wheat. The main objectives of this study were: 1) To determine the effect of Sm1 on grain yield and end-use quality. 2) To genetically map the Sm1 introgression. For this work, 122 F5:9 recombinant inbred lines (RILs) derived from a cross between the midge susceptible durum wheat cultivar CDC Verona (Sm1 “-”) and resistant experimental line DT780 (Sm1 “+”). Agronomic and end-use quality traits of the mapping population were analyzed. The results from each environment were used for quantitative trait loci (QTL) analysis at Kernen (SK) in 2009 and 2010, and at Indian Head (SK) in 2009. On average, the presence of Sm1 was associated with higher grain yield and yellow pigment content, but lower kernel weight, reduced grain protein content, and weaker gluten properties. However, it was possible to identify RIL lines carrying Sm1 that expressed higher kernel weight, grain protein content, and stronger gluten. A genetic linkage map spanning 58 cM on chromosome 2B near Sm1 was constructed. QTL mapping suggested that the total length of the Sm1 introgression into durum wheat was approximately 11cM. Nearly all traits measured showed QTLs associated with Sm1. For grain protein content, a QTL proximal to Sm1 was identified, suggesting that Sm1 per se may not be contributing to the reduced grain protein observed in the Sm1 carriers of the RIL mapping population. The results presented here suggest that on average, Sm1 is associated with higher grain yield and some reduced end-use quality factors, but that it may be possible to combine Sm1 with high grain yield and end-use quality equivalent to current check cultivars.

La faible efficience des ressources non renouvelables comme l’eau et l’azote, constitue un frein à un accroissement durable de la production des céréales. L’augmentation simultanée de l’efficience de l’utilisation de ces deux facteurs et des rendements constitue un défi majeur pour la production céréalière en méditerrané, principalement la production de blé dur. L'objectif de la thèse est d’analyser la variabilité des rendements de blé dur en Tunisie vis-à-vis des deux facteurs de production l’eau et l’azote et les possibilités d’augmenter conjointement la productivité et l’efficience dans les systèmes de culture à base de blé dur. Cette étude s’appuie sur des données expérimentales, de statistiques régionales et d’enquêtes entreprises auprès des agriculteurs sur 537 situations agronomiques (la combinaison d’une parcelle de blé dur, une année et un précédent cultural) de blé dur dans 4 régions qui fournissent une partie importante de la production céréalière. Les données collectées nous ont permis d’établir un cadre d’analyse des compromis entre l’efficience de l’utilisation de la ressource (eau et azote) et le rendement, en se basant sur l’approche de la décomposition de l’efficience et les concepts de facteur limitant, de frontière d’éco-efficience et d’écart de rendement. Testé avec des données expérimentales et appliqué sur les données d’agriculteurs, pour le cas de l’azote, ce cadre a démontré qu’avec un précédent non céréalier, particulièrement des légumineuses, on peut obtenir un potentiel d’absorption d’azote beaucoup plus élevé qu’en monoculture. Les précédents culturaux diversifiés ont permis aussi d’augmenter simultanément la production et l’efficience de l’utilisation d’azote. En revanche, l’augmentation de la frontière d’efficience de la captation d’azote a été marquée par un accroissement des écarts d’absorption à cette frontière, indiquant un effet plus marqué des autres facteurs limitants dans le cas de rotations diversifiées. L’eau et ses interactions avec l’azote pourraient être considérées parmi les principaux facteurs qui peuvent affecter l’absorption azotée et par conséquent les rendements de blé dur. Ce travail permettrait de combiner d’une part le diagnostic des facteurs limitant la production au niveau des parcelles d’agriculteurs et d’autre part l’analyse des scénarios de gestion des compromis production efficience avec les agriculteurs et les décideurs. / The low efficiency of non-renewable resources such as water and nitrogen is a constraint for a sustained increase in cereal production. The simultaneous increase in water and nitrogen use efficiency and yields is a major challenge for cereal production in the Mediterranean, mainly for durum wheat production. The aim of the thesis is to analyze the variability of durum yields in Tunisia with respect to the two factors of production water and nitrogen and the opportunities of jointly increasing productivity and efficiency in a durum wheat-based cropping systems. This study is based on experimental data, regional statistics and farm surveys conducted on a sample of 537 agronomic situations (the combination of a farmer’s field, a year and a previous crop) which are representative of the diversity of farmers’ practices in four grain-producing regions that provide a major part of cereal production. Based on the efficiency decomposition approach and the concepts of limiting factor, eco-efficiency frontier and yield gap, data collected allowed us to establish a framework allowing to analyze trade-offs between the resource (water and nitrogen) use efficiency and yield. Tested with experimental data and applied to farmers' data for the case of nitrogen, this framework demonstrated that non-cereal preceding crops, especially legumes, result in a higher potential for N uptake by durum wheat than with a monoculture. Diversified rotations result also to an increase in both yield and N-use efficiency of the following durum wheat. The increase in the efficiency frontier of nitrogen capture is partly of-set by increased N uptake gaps in farmers’ fields indicating the presence of other limiting factors. Water and its interactions with nitrogen could be considered among the main factors that may affect nitrogen uptake and hence yields of durum wheat. This work would allow combining, on the one hand, the diagnosis of limiting factors at the level of farmer’s fields and on the other hand the analysis of scenarios for the management of trade-offs between production efficiency with farmers and decision-makers.