The main target of the master thesis “GIS application for marking of cycle paths” is to investigate the opportunities of cycle tourism in Lithuania, and to mark the cycle tour route and cycle route as technical design using GIS technology. The cycle route designing principles, marking possibilities In Lithuania and abroad, also cycle rout designs and cyclists flows are analyzed while estimating the opportunities of cycling tourism. Basing on the collected data and the implemented pools,, the GIS sub-system of the cycle tour rout, and its’ development technology is described. Such information would be useful for the cyclists while planning the tour rout and during the tour, however such system would be even more useful if there would be more marked and classified cycle paths. After creating the common Lithuanian cycle paths database we could gather and propose an information on the cycle tourism possibilities to the cyclists, which could benefit further development of tourism in Lithuania. The Dzukija cycle route technical design data were used during development of GIS subsystem of cycle paths. Herefore the rout were marked by hands. The developed GIS subsystem allows wider information appliance. The work consists of 76 pages, 16 tables, 43 pictures, 5 annexes, 29 sources.