In this project we investigate the feasibility of detecting the signatures of Pop III stars in metal poor second generation stars and in gas clouds at high redshifts. First, the nucleosynthetic yields of Pair Instability Supernova and how they are manifested in gas clouds are presented. Next, some basic quantities of radio astronomy are explained and the requirements of SKA are shown. Then, the minimum detectable hydrogen column density of SKA for gas clouds at high redhsift is calculated and after that the basic principles of spectroscopy and the requirements of the HiReS instrument of E-ELT are demonstrated. Finally, suggestions about where the observations with HiReS should focus are made.