Student Number :0318744F - MA research report - School of Arts - Faculty of Humanities / Title: LIVING DANGEROUSLY Subtitle: HIV/Aids, masculinity and the post-apartheid generation: A case study AIM: to investigate via the story of one young South African man the complexity of dealing with HIV/Aids in South Africa. RATIONALE: With the ending of apartheid and the rise of HIV/Aids, there has been a clear crisis of masculinity in the wake of social change. Government response to the epidemic has been ambiguous. Fana Khaba, aka Yfm DJ Khabzela, was the first young black celebrity to publicly declare he had Aids. I plan to follow his story and to look at HIV/Aids campaigns and to examine why they are not working. METHODOLOGY: Through a literature review, an examination of statistics and public health messaging on HIV/Aids and my investigation into the life of Fana Khaba, I will show the complexities currently not being considered in the compilation of public health messaging. The reason I chose to follow the story of Fana Khaba is because I am a South African deeply concerned about HIV/Aids. I found his life compelling because it encapsulated so much of the rapid and intense culture shift that followed the arrival of democracy in 1994. And because his life echoed that of a pivotal generation in the apartheid struggle: the generation who grew up in Soweto in the seventies and eighties and came to adulthood with democracy. The so-called “lost generation” who later became known as the “Y generation”, they are deeply affected by the pandemic. I intend to show that Fana Khaba was a hugely popular iconic figure for the generation because he spoke to their aspirations and their anxieties. I will argue that because his life experience resonated so strongly with this generation, it is reasonable to draw more general lessons from it. The chief executive officer of Yfm was a friend of mine and, through him, I am able to gain access to Khabzela, his family, friends and colleagues at Yfm. This is an exceptional opportunity to gain an inside view of a life not readily available to relative outsiders such as myself. Clearly there is an ethical issue here. I will at all times keep my interviewees informed about the purpose of my research. I hope to help shed light on this anguished, important and under-debated sphere of life in South Africa.. The format I choose is part investigative journalism, part biography. The reason for this is that I have worked as a journalist for 25 years so all my skills and training point me in that direction. I wanted to make it accessible in order to reach as many people as possible. The narrative-biographical form is conducive to this because it is easy to engage with. In order to give the narrative tension and focus, I shall repeatedly employ the central question of why Fana Khaba refused to take the anti-retrovirals which might have saved his life.

This thesis is part of a creative arts PhD which explores the possibilities of constructed images and the memory of the South African Border War. It was presented together with an exhibition of constructed photographic images entitled BOS. In the thesis I argue that the memory of the war, an event now almost three decades past, continues to be problematic. I also argue that photographs are themselves complex and constructed objects that do not provide a simple truth about either history or memory. Photographs can supplement or support memories but they are always to be viewed with suspicion. In Chapter One I explore the limitations imposed on the speech of conscripts, both during the conflict and in the years following the conclusion of hostilities. In Chapter Two I examine the recent appearance of several ‘anti-­‐ heroic’ memoirs of the conflict written by conscripts. The use of the medical diagnosis of post-­‐traumatic stress syndrome (PTSD) in these writings is critically examined. Chapter Three focuses on a development in the ideas of the two most influential figures in the field of Anglophone photographic theory, Susan Sontag and Roland Barthes. I argue that their initial hostility to the photographic image on ethical/political grounds has been replaced by a more nuanced engagement with the power of the image. I then examine the views of two contemporary writers on photography, both deeply involved with the analysis of traumatic images: Ariella Azoulay and Susie Linfield. In Chapter Four, I engage with the artistic practice of the American photographer, David Levinthal, an important reference point for this project because of his photographic work with miniatures and toys and his place within what I describe as ‘critical postmodernism’. In Chapter Five, I examine the themes of silence and censorship as these pertain to the photography of the Border War using Susan Sontag’s notion of the “ecology of images”. I analyze the types of images which have been produced from the war, looking at the “limited photojournalism” of John Liebenberg and the role of iconic images in the propaganda war. Finally, in Chapter Six, I present an account of the process of creating the work for the BOS exhibition in which I employed a combination of strategies involving appropriation, miniaturization, and re-­‐staging.

Blood flow to the previously active skeletal muscle remains elevated for several hours following an acute bout of aerobic exercise and is dependent on activation of H1 and H2 histamine receptors. Many questions remain unanswered in humans regarding the mechanisms mediating this sustained post-exercise vasodilation and what benefits come of this physiological phenomenon. The studies detailed in this dissertation were designed to examine the upstream mechanisms and explore a potential benefit associated with sustained post-exercise vasodilation. In chapter IV, we examined if oxidative stress is the upstream exercise-related factor mediating sustained post-exercise vasodilation. Intravenously infusing the antioxidant ascorbate blunted sustained post-exercise vasodilation, and this reduction was similar in magnitude to that observed with H1/H2 blockade. However, ascorbate may directly degrade histamine and may also inhibit its formation. Therefore, we conducted a follow-up study to verify the findings in study 1. In this study, we intravenously infused n-acetylcysteine, a potent antioxidant with no known histaminergic interactions. We found that n-acetylcysteine had no effect on sustained post-exercise vasodilation, indicating that exercise-induced oxidative stress is not the exercise related factor mediating sustained post-exercise vasodilation. In chapter V, we attempted to measure interstitial histamine in an effort to demonstrate that exercise induces the local formation of histamine in previously active skeletal muscle. We found that histamine is increased in the interstitial fluid within skeletal muscle during and after exercise. Additionally, we determined that de novo synthesis via histidine decarboxylase contributes to the rise in histamine during and following exercise. We also demonstrated a possible role of mast cells as an additional mechanism augmenting histamine in skeletal muscle. Collectively, these studies demonstrate that histamine is the ligand activating histamine receptors and activation is due to the induction of histidine decarboxylase and mast cell activation. In chapter VI, we attempted to determine if histamine receptor activation contributes to the expression of pro- and anti-angiogenic growth factors during the recovery from exercise. Our preliminary findings indicate that activation of histamine receptors may play a role in the expression of pro-angiogenic growth factors during the recovery from acute aerobic exercise.

This dissertation consists of three different essays evaluating solutions to postharvest challenges faced by farmers in Kenya and Tanzania. In the first essay we see that demand for a new storage technology the Purdue Improved Crop Storage (PICS) bags in Western Kenya, a completely new technology for almost the entire sample, was highly elastic and that a small proportion of the population would buy at the current market price. In the second essay we find evidence that farmers, who are primarily growing for maize consumption are more concerned about food safety in maize than traders, who are willing to pay less to keep the maize safer. In the third essay in Tanzania, we find that liquidity concerns at harvest prevent farmers from optimizing maize storage and sales decisions.

Philosophiae Doctor - PhD / As a literary intervention, The Post-Genocidal Condition: Ghosts of Genocide, Genocidal Violence, and Representation is situated at the intersection of genocide studies, psychoanalysis, and literature so as to enable a critical engagement with the question of genocide and an attempt to think beyond its formulation as phenomenon. As the dominant framework for thinking genocide within international jurisprudence, and operating as the guiding terrain for interventions by scholars such as Mamood Mamdani, Linda Melvern, and William Schabas, the presumption that genocide may be reduced to a marked beginning and end, etched out by the limits of its bloodiness, is, I argue, incomplete and thus a misdiagnosis of the problem, to various effects. Moreover, I contend that it is this misdiagnosis that has led to what I name as the post-genocidal condition: a deferred return to the latent violences of genocide; enabled often through various mechanisms of transitional justice. This intervention is not a denial that under the rubric of the crime of genocide, as an attempt to destroy in whole or in part what Raphael Lemkin referred to as an “enemy group”, millions of people have died. Rather what I posit is that the physical violence of genocide is a false limit – that the bloodiness of genocide has been mistaken for the thing-in-itself. Thus this intervention is an attempt to offer another way of thinking the question of genocide by reading it as concept, enabling a consideration of its more latent violences, its ghosts. As such, I argue that genocide is first an attack on the minds of the persons who form the targeted people or group, through the destruction of cultural apparatuses, such as books, works of art, and the language of a people, to name but a few; and is lastly an attempt to physically exterminate a people. Thus this intervention invites a return to Lemkin’s formulation of the term in Axis Rule in Occupied Europe: Laws of Occupation, Analysis of Government, Proposals for Redress (1944); that the word genocide is meant to “signify”, and as such offers a reading of the question of genocide as signifier, understood, I suggest, in the Lacanian sense. Thus, I posit that genocide, as signifier, operates on both the levels of metaphor and metonym, and as such both condenses and displaces its violence(s). The metaphor for genocide as signifier is, furthermore, rather than the signifying chain as Lacan would have it, the network. As such genocide is marked as text, rather than work; its perpetrators not authors, as Lemkin and various pieces of legislation have described them, but writers; and those who engage with the question of genocide, to whatever degree, as readers rather than critics. Consequently, this intervention stages the question of the reach of impunity and complicity, beyond the limit of judicial guilt and innocence. Metonymically, the relational displacement at work within the network of genocide allows for a reading of the various constitutive examples of the violence(s) that, in combinations and as collective, produce a new signification, other than that of the definitional referent.

Mating in insects influences suites of behavioural and physiological changes in females. These changes can include key female traits such as dispersal, foraging, oviposition and female remating or receptivity. Whilst much is known at the phenotypic level about post-mating changes in reproductive biology across many species, much less is known at the genetic level, especially outside of established model organisms such as Drosophila melanogaster. In the parasitic wasp Nasonia vitripennis courtship behaviour, rather than copulation, is believed to be primarily responsible for driving changes in female post-mating behaviour. Here we have studied female receptivity and post-mating gene expression changes associated with courtship and copulation in Nasonia vitripennis. Firstly we considered the influence of the duration of various elements of courtship and mating on female re-mating rates. We were able to identify an association between long pre-copulatory courtship durations and females which are less likely to re-mate (after 24 hours) and suggest that this may be driven by females which are generally less receptive. We also observed that males may be capable of determining female mating state, taking longer to engage in courtship with mated females than virgin females. To further explore the influence of mating on female post-mating behavioural and physiological processes, we explored changes in gene expression occurring in response to mating. To do this we utilised two different transcriptomic sequencing approaches developed for the Illumina next-generation sequencing platform. Using a tag-seq approach we considered the differential gene expression occurring in response to mating in head and body (comprising of the thorax and abdomen) tissues across two time-points (30 minutes and four hours). We were able to identify large changes in expression in head tissues across time-points in comparison to more subtle changes in body tissues. We suggest that head tissues may be more closely associated with post-mating changes in behaviour, whilst body tissues are perhaps physiologically more associated with egg production and influenced less by mating per se. Finally, using an RNA-seq approach, we considered the gene expression changes occurring in female body tissues in response to three elements of male courtship across two time-points (30 minutes and 24 hours). We hoped to narrow down the role of male courtship and/or insemination in post-mating gene expression differences, addressing first the more limited changed in body tissues. We showed that time-point was the most important factor associated with post-mating gene expression, with the courtship components tested being associated with very little expressional change. The data presented in this thesis suggests that male courtship may not be that important for driving the post-mating behavioural and genetic changes seen in Nasonia, perhaps limiting the scope for sexual conflict over reproduction in this species.

This thesis studies the intersection between contemporary international political theory and global environmental politics. It asks whether concern for global environmental degradation requires a rethinking of the assumptions that underlie international political theory as a field of study within the discipline of International Relations. Answering this question, the thesis introduces three ‗images‘ of international political theory: the liberal cosmopolitan, the critical-theoretical, and the anti-foundationalist. It investigates the contributions of these three images of international political theory to global environmental politics. Assessing, through the three images, the status of contemporary international political theory in light of environmental concerns, the thesis suggests that while international political theory offers many important insights into discussions of global environmental politics it also appears significantly limited when dealing with environmental concerns. Key among its limitations is the human-centred framework and mission of contemporary international political theory that an encounter with environmental concerns helps expose. The thesis argues that international political theory, both to be true to its purpose – that is, the extension of moral and political inclusion in world politics – and to maintain its relevance in the contemporary world, must seek a more thorough engagement with environmental concerns. The thesis contends that a fundamental rethinking of the assumptions that underlie contemporary international political theory forms an important – and necessary – part of this engagement.

The thesis explores current debates ,around postfeminism and neoliberalism, and young women's articulations of femininity within the context of young women's excessive drinking practices. Alcohol plays a key ro le in UK culture today, and for young people, getting drunk is an accepted, expected and indeed normalised part of a night out in the current 'culture of intoxication'. It is also a space for enacting highly visible displays of gender, femininities and class, and one that represents an important 'space of attention' for exploring contemporary subjectivity. As such this space provides a productive source for carrying out in-depth analysis of how young women negotiate and manage 21st century femininities in the UK. Data is provided in the form of white working-class women's accounts of excessive drinking in various drinking venues within the county of Hampshire, England. Thirty-three women, aged between 18 and 24 years, took part in several phases of data collection, and these include individual interviews, friendship group discussions, and ethnographic methods. I employed a version of Foucauldian discourse analysis to identify key themes and discourses in the young women's talk, and note how young women use excessive alcohol for confidence within what has become a drinking culture of hyper-sexuality, where the emphasis is on the traditional male gaze, but also and possibly even more powerfully, the postfeminist female gaze. The young women draw on a number of discourses to construct drunkenness as a routine part of going out, and how the female gaze plays an important role in 'mirroring' and/or 'othering' women in terms of their feminine recognition. Furthermore, the women draw on postfeminist discourses to emphasise how painful and hard it is becoming a young female subject today.

"The purpose of this project is to design a non-snagging guardrail post. The procedure will be to first develop a simple finite element (FE) model of a single post, wheel and suspension to explore the snag potential for some existing standard guardrail posts. The next step in the procedure will be to develop appropriate design changes that could prevent wheel snagging and investigate if they do by using a one-post sub-model. An attempt to validate the used material model for wood will also be done by comparison between laboratory tests and finite element simulations."

OBJECTIVE: Osteoarthritis (OA) is a leading cause of disability worldwide. This disease is characterized by the inflammation and degradation of the cartilage and surrounding tissue in a joint. The disease manifests as either a result of years of wear and tear or after a joint injury. Post-traumatic osteoarthritis, as this latter case is named, is frequently studied since the exact trigger of the disease is known. In addition to several changes within the joint space, a significant alteration is the degradation of cartilage caused primarily by the release of inflammatory cytokines including interleukin-1 and 6 and tumor necrosis factor α. One current pharmacological treatment for the pain caused by OA is an intra-articular injection of glucocorticoids such as dexamethasone. As this is a common treatment, the goal of this research was to determine if, at the cellular level, this treatment impacts cell viability in the presence of pro-inflammatory cytokines. Another goal was to investigate how such treatment affects the progression of cartilage degradation caused by cytokines. OA results in the loss of the key extracellular matrix molecule, aggrecan, which contains negatively charged glycosaminoglycan (GAG) chains. Measurement of the amount of GAGs lost is an early indicator of cartilage degradation. In addition, biosynthesis of GAG chains can be measured to estimate the overall metabolic health of the cells. We hypothesized that dexamethasone blunts the harmful effects of proinflammatory cytokines and improves GAG biosynthesis and chondrocyte viability. METHODS: Cylindrical cartilage explants were collected from bovine knee joints and trimmed to a uniform 3 millimeters in diameter and 1 millimeter thick. Each treatment group consisted of n=6 explants from the same knee joint. In one set of experiments, these explants were subjected to two different doses of interleukin-1α (1 ng/mL and 10 ng/mL) with and without dexamethasone at 100 nM. In another set of experiments, explants were subjected to both interleukin-1α and tumor necrosis factor-α (1 ng/mL and 25 ng/mL respectively). The explants were cultured in medium for 6 days and were digested for outcome measurements on the final day. On day 4, 35S-sulfate was added to the explant medium for later measurement of radiolabel incorporation as a measure of GAG biosynthesis. Cell viability was measured on day 5 using red/green fluorescent viability dyes fluorescein diacetate (FDA) which stains live cells green and propidium iodide (PI) which stains dead cells red. RESULTS: Compared with untreated controls, explants subjected to the pro-inflammatory cytokines interleukin-1α and tumor necrosis factor-α exhibited greater glycosaminoglycan loss and a decrease in GAG biosynthesis. These treatments also decreased cell viability. Addition of dexamethasone improved cell viability compared to treatment with the cytokines. In addition, dexamethasone prevented glycosaminoglycan loss and increased GAG biosynthesis in the presence of interleukin-1α. However, dexamethasone did not prevent tumor necrosis factor-α mediated loss of GAGs. CONCLUSION: These studies demonstrated that dexamethasone inhibited specific aspects of cartilage degradation associated with inflammation in early OA. This therapeutic counteracts the degradative changes initiated by inflammatory cytokines such as interleukin-1α without compromising cell viability. Future studies are needed to identify the mechanisms of dexamethasone action and the ideal concentration to use if it is to be used as a treatment for OA following acute joint injury.