Tetraciklinų nustatymas piene atliktas efektyviąja skysčių chromatografija ir įteisintas remiantis ES išleista direktyva 2002/657/EB. Metodas atitinka visus kriterijus aprašytus šioje direktyvoje. Antibakterinių medžiagų likučiai iš pieno ekstrahuojami Mcllvaine-EDTA buferiniu tirpalu, o po to valymas atliekamas naudojant OASIS HLB kietafazės ekstrakcijos kolonėles. Chromatografinėje kolonėlėje atskyrimas atliekamas naudojant 10 mM oksalo rūgšties vandeninio tirpalo ir ACN gradientinį režimą, tėkmės greitis 0,9 ml/min, 100 μl injekcija, bangos ilgis 355 nm ir optimali kolonėlės temperatūra (LiChroCART Superspher 60 RP C8 (250 x 4,6 mm, dalelių dydis 5 m)) – 30 oC. Visi rezultatai apskaičiuoti naudojant kompiuterinę duomenų apdorojimo programą “Interval”. Tetraciklinų išgavos nuo 86,20 % iki 107,19 %, o nustatymo ribos yra intervale nuo 26,7 μg/l iki 49,3 μg/l. / Analysis of tetracycline compounds was performed using high performance liquid chromatography and validated according to EU commition decision 2002/657/EC. Method is compliant at all criteria described in the decision. Antibacterial material residues were extracted from milk with Mcllvaine-EDTA buffer followed by clean-up on OASIS HLB cartridges. Separation was performed using 10 mM oxalic acid/ACN gradient with flow rate 0.9 ml/min, 100 μl injection, detection at 355 nm and the optimal temperature of column (LiChroCART Superspher 60 RP C8 (250 x 4.6 mm, size of particles 5 m)) – 30 oC. All the results were evaluated statistically with “Interval” data processing program. Recoveries are at the range from 86.20 % to 107.19 %. The determination limits for tetracyclines were carried out from 26.7 μg/L to 49.3 μg/L in this study.

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Every year Malaria ruins millions of lives. Can we change it? There are more and more resistant Malaria forms so efficacy of drugs is lesser. I participated on non clinical trials with new malaria drug in France, Montpellier1 University, faculty of pharmacy, clinical pharmacokinetic laboratory. It’s always necessary investigate pharmacokinetic parameters (clearance, half-life, volume of distribution etc.) in study with animals. Can we calculate these parameters to human using parameters of animals? In this work you can find malaria aetiology and epidemiology, the ways how to protect from this disease and revue of drugs for treatment. There you can find structure-activity action of new molecule. Also there explained choice of analytical method and allometry. In practice part of my work there is quantity analysis and preparation for it, stability and method validation results. Analytical samples prepared with pig’s, dog’s, monkey’s, rat’s and mouce’s plasma. All calculated pharmacokinetic parameters were used in allometric analysis and extrapolation to the man. The main tasks of my work were: • Calculate concentration and pharmacokinetic parameters for animals; • Verify allometric relation for the new drug; • Calculate pharmacokinetic parameters for the man by using allometric methods. From obtained information we can indicate prior drug absorbsion, metabolism and efficacy to the man.

Thesis (M.S.)--Wisconsin State University (La Crosse), 1967. / Includes bibliographical references (leaves [167-173]).