A maioria dos hidrozoários é marinha, mas eles também são frequentemente encontrados em estuários. Estes ambientes são considerados um dos principais em número de invasões biológicas. A fauna brasileira de hidrozoários estuarinos é praticamente desconhecida, exceto por alguns registros esparsos. Os objetivos deste estudo são (1) inventariar a fauna de hidroides planctônicos e bentônicos em quarto estuários, Cananéia, Paranaguá, Guaratuba e Babitonga das regiões Sudeste e Sul do Brasil, investigando as afinidades entre os estuários e também a relação entre pólipos estuarinos e seus substratos; (2) caracterizar e comparar as comunidades de hidrozoários planctônicos e bentônicos no gradiente salino (3) e entre o verão (estação chuvosa) e o inverno (estação seca); e (4) inferir padrões que possam auxiliar no entendimento das invasões dos medusozoários. As amostras foram realizadas nos verões e invernos de 2007, 2008 e 2009, em seis isohalinas, sempre que possível. Nós também copilamos dados da literatura com registros de espécies invasoras e criptogênicas e contrastamos estes registros com as características biológicas das espécies. Nós identificamos 37 espécies de hidrozoários (21 medusas e 17 pólipos). Liriope tetraphylla foi a medusa mais frequente em amostras nos estuários de Cananéia e Guaratuba, mas Clytia spp. e Blackfordia virginica foram as medusas mais abundantes nas baías de Paranaguá e Babitonga, respectivamente. Bougainvillia muscus, Clytia gracilis e Obelia bidentata foram as espécies de pólipos mais frequentes em todos os estuários. Os pólipos frequentemente utilizaram cracas, bivalves, hidrozoários e partes de mangue como substrato. Clytia gracilis foi a espécie encontrada sobre maior número de substratos diferentes, enquanto Acharadria crocea foi encontrada mais frequentemente sobre substratos artificiais. Entre as Entre as medusas, Ectopleura dumortieri foi encontrada apenas na salinidade mais alta (30) em todos os estuários, enquanto Moerisia inkermanica foi encontrada apenas nas salinidades 10 e 15. Entre os pólipos A. crocea restringiu-se a salinidade 30 em todos os estuários. A maioria das espécies de medusas (67%) e de pólipos (65%) amostrada era eurialina, ocorrendo em pelo menos quatro salinidades diferentes. As comunidades das medusas variaram sazonalmente, principalmente pela variação na abundância das espécies mais ubíquas. Enquanto as comunidades de pólipos parecem ter sido menos influenciadas pelas estações, exceto por A. crocea que ocorreu apenas no inverno. Nós listamos 43 (37 Hydrozoa e 6 Scyphozoa) como invasoras e 15 espécies de Hydrozoa como criptogênicas. Nós observamos que alguns padrões de invasão nos Medusozoa podem estar relacionados à biologia das espécies e com a filogenia dos grupos. / The majority of the hydrozoans is marine, but they are also often recorded in estuaries. This environment is considered one of the most important in number of biological invasions. The Brazilian brackish hydrozoan fauna is practically unknown, except for some scattered records. The goals of this study are (1) to survey the planktonic and benthic hydrozoans fauna in four estuaries, Cananéia, Paranaguá, Guaratuba e Babitonga, from southern Brazil, investigating the affinities among these estuaries and also the relationship between estuarine polyps and their substrates; (2) characterize and compare the planktonic and benthonic hydrozoan communities in salinity gradients (3) and between summer (raining station) and winter (dry station); and (4) infer patterns that might help to understand Medusozoan invasion. Samplings were carried out during summer and winter of 2007, 2008 and 2009, at six isohalines for each estuary, whenever it was possible. We also compiled data of literature records of invasive and cryptogenic species and contrasted those records with biological features of these species. We have recorded 37 species of hydrozoans (21 medusa and 17 polyps). Liriope tetraphylla was the most abundant and frequent medusa in the samples of Cananéia and Guaratuba estuaries, but Clytia spp. and Blackfordia virginica were the most abundant medusae in Paranaguá and Babitonga bays, respectively. Bougainvillia muscus, Clytia gracilis and Obelia bidentata were the most frequent polyps in all estuaries. Polyps frequently used barnacles, hydrozoans, mussels shells and mangrove parts as substrates. Clytia gracilis was the species recorded in the largest number of substrates, while Acharadria crocea was recorded more often on artificial substrates. Among the medusae, Ectopleura dumortieri was found only in the highest salinity (30) in all estuaries, while Moerisia inkermanica was recorded in the salinities 10 and 15. Among the polyps, A. crocea was constrained to salinity 30 in all estuaries. The majority of the species of medusa (67%) and polyp (65%) sampled was euryhaline, occurring in at least four different salinities. Communities of medusae vary seasonally, mainly by the abundance variation of the most ubiquitous species. Whilst the community of polyps seems to be less influenced by season, except for A. crocea that occurred only in winter. We have listed 43 (37 Hydrozoa and 6 Scyphozoa) species recorded as invaders and 15 hydrozoan species record as cryptogenic. We have observed that some patterns of invasion in Medusozoa may be related with the species\' biology and with the phylogeny of the group

Orientador: Sueli Yoshinaga Pereira / Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Geociências / Made available in DSpace on 2018-08-26T07:20:33Z (GMT). No. of bitstreams: 1 Gasparini_FelipeBalieiro_M.pdf: 60117835 bytes, checksum: bc9cbbddb75978f83fffc1bc63e6b3ab (MD5) Previous issue date: 2014 / Resumo: O estudo hidrogeológico de aquíferos estuarinos em áreas industriais apresenta complexidade em seu entendimento devido às interações físicas naturais e/ou antrópicas que interferem na sua dinâmica, tais como as flutuações da maré, alterações do regime de rios, mudanças sazonais das chuvas, e os sistemas de remediação instalados nas áreas impactadas. A presente dissertação visa, portanto, caracterizar os tipos de aquíferos e a dinâmica das águas subterrâneas em um ambiente estuarino adjacente ao rio Cubatão situado no município de Cubatão/SP, em uma área sob influência da maré e processos industriais. A pesquisa abordou os seguintes estudos: (1) caracterização e levantamento do histórico do uso da área pela indústria; (2) caracterização da geometria e monitoramento das águas dos aquíferos locais; (3) análise do monitoramento dos parâmetros climáticos como pressão e temperatura do ar atmosférico e intensidade das chuvas, e; (4) monitoramento hidrológico e da influência da maré oceânica no rio Cubatão. O comportamento dos aquíferos (livre e confinado) e do rio Cubatão foi monitorado nos períodos chuvoso (novembro de 2012 a janeiro de 2013) e pouco chuvoso (julho e outubro de 2013). Em síntese, os resultados não indicaram influência das chuvas na recarga do aquífero livre. No entanto, existe uma forte relação entre o aquífero livre, as alturas da maré oceânica no rio Cubatão e a injeção de ar pelo sistema de remediação. Há o predomínio da dinâmica natural do rio Cubatão, seja em períodos de vazante como de cheia. O trecho do rio Cubatão na área pesquisada é influente em relação ao aquífero livre, devido a relação da curvatura desse e à diferença das cargas hidráulicas entre o rio e o aquífero, excetuando os momentos de operação do sistema de remediação do módulo mais próximo. Os valores de condutividade elétrica apresentam-se similares entre o rio Cubatão e o aquífero livre, e baixos nas águas do aquífero confinado, indicando a influencia do rio com o aquífero no primeiro caso e a descarga de águas doces regionais no segundo. Este trabalho contribuiu para o melhor entendimento da dinâmica das águas subterrâneas em aquíferos estuarinos sob forte influência de condicionantes externas, como variação do rio e da maré e operações de atividades antrópicas (sistemas de remediação de contaminantes) / Abstract: The complexity of hydrogeological studies of estuarine aquifers on industrial areas is due to natural and/or anthropogenic physical interactions that interfere on its dynamics, such as tide fluctuation, changes in the state of rivers, seasonal rain variation rates, and remediation systems installed on impacted areas. This work aimed to characterize the aquifer types and the groundwater dynamics on a estuarine environment adjacent to Cubatao river, located on Cubatao city (Sao Paulo State, Brazil), on an area influenced by tides and industrial processes. The research approached the following topics: (1) characterization and survey of the area¿s historical use by the industry; (2) hydrogeological characterization and monitoring of the local aquifers; (3) climate parameters analysis, as pressure and temperature of the atmospheric air and rain intensity; and (4) monitoring of the hydrology and the tide influence over Cubatao river. The behavior of the unconfined and confined aquifers and the Cubatao river was monitored on rainy (November of 2012 to January of 2013) and less rainy (July and October of 2013) periods. In summary, the results indicated no rain influence on the unconfined aquifer recharge. However, there is a strong relation between the unconfined aquifer, the oceanic tide heights on Cubatao river and the air injection by the remediation system at the industry plant. Nonetheless, the influence of natural dynamics on the Cubatao river prevail, whether in periods of high or low tides. The stretch of the Cubatao river at the study area is characterized as a losing river in relation to the unconfined aquifer, due to its curvature and the hydraulic head difference between the river and the aquifer, except during operational moments of the nearest module of the remediation system. Moreover, the electrical conductivity values were similar between the Cubatao river and the unconfined aquifer, and lower on the confined aquifer waters, indicating the river influence over the aquifer on the first case and the discharge of fresh regional water on the second. Finally, this research contributed to improve the understanding of estuarine aquifers dynamics under strong influence of external factors, such as river and tide variation and anthropic activities (contaminant remediation systems) operation / Mestrado / Geologia e Recursos Naturais / Mestre em Geociências

The overarching goal of this thesis is to develop a diagnostic and predictive model to quantify the estuarine CO2 dynamics across scales – from catchment to the globe – using an approach that explicitly resolves the strong physical and biogeochemical gradients typically observed in these systems.Chapter 1 provides fundamental definitions and descriptions of estuaries, as well as an assessment of their role in the global carbon cycle. It also raises the specific objectives and research questions tackled in the present study. Chapter 2 presents the rationale behind the novel modelling approach (C-GEM, Carbon-Generic Estuary Model) developed in the framework of this thesis. First, the dominant processes that control the estuarine biogeochemistry in estuaries are discussed in detail. Then, the power of reactive-transport models (RTMs) in understanding and quantifying the estuarine biogeochemical functioning is illustrated on the basis of local modelling studies. Finally, trends in estuarine biogeochemical dynamics across different geometries and environmental scenarios are briefly explored with C-GEM and results are discussed in the context of improving the modelling of estuarine carbon dynamics at regional and global scales. In Chapter 3, a detailed description of C-GEM, both in terms of structure and set-up, is provided and model’s performance is successfully evaluated through comprehensive model-data and model-model comparisons in the macro-tidal Scheldt estuary (BE/NL). In Chapter 4, C-GEM is combined with a generic set of forcing conditions and parameter values to quantify the carbon dynamics (net ecosystem metabolism, CO2 exchange at the air-water interface, carbon filtering capacity) in three idealized estuaries subject to temperate climatic conditions. Their hydro-geometrical characteristics span the wide diversity of estuarine morphological characteristics. Model results are used to upscale the estuarine CO2 dynamics under present-day conditions and to quantify the response of the estuarine filter to future atmospheric CO2, land use and climate change scenarios. In Chapter 5, C-GEM is applied to derive estimations of carbon export and CO2 outgassing from all tidal estuaries discharging in the North Sea. Overall, our results suggest that the estuarine carbon filtering capacity and the contribution of these land-ocean transition systems to the atmospheric CO2 budget might not be as high as previously thought. Finally, a conclusive chapter (Chapter 6) provides a synthesis of the key findings and arguments projected by the present research work. Moreover, recommendations are given in the light of further applications of the modelling approach developed during this thesis. / Doctorat en Sciences / info:eu-repo/semantics/nonPublished

There is a growing interest in tidal energy, owing to its predictable nature in comparison to other renewable sources. In the case of the UK, its importance also lies on the availability of exploitable areas as well as their total capacity, which is estimated to cover more than 20% of the country demand. However, the level of development of this kind of technology is still far behind other types of renewable energy. However, several studies focused on a variety of individual devices, followed by more recent research on the deployment of large arrays or tidal farms. Potential sites for energy extraction can be found in narrows between islands and the coast or estuaries. The latter present some advantages for the installation and the connection to the grid but estuaries are often prone to flood risk from tides and surges. Therefore, the objective of this thesis is to evaluate the effect that very large groups of turbines could have on peak water levels during flooding events in the case of being deployed in estuarine areas. For that purpose, a new methodology has been developed, which implies the use of a numerical model (MIKE 21 by DHI), and it has been demonstrated against a real case study in the UK: the Solway Firth estuary. Another objective has consisted of integrating in this thesis the results from detailed CFD modelling and optimisation techniques involved in the project. A literature review has been carried out in order to identify the current state of the art for the different subjects considered in the thesis. Different aspects of the numerical model used for this study (MIKE 21) have been presented and the modelling of the turbines within the code has been validated against experimental and CFD data. The procedure to include large numbers of turbines in the code is also developed. An analysis has been done of the different estuaries existing in the UK suitable for tidal energy extraction, identifying their main geometrical features. Based on this, idealised models of estuaries have been used to assess the influence that the channel geometry could have on the impact of tidal farms under extreme water levels. The effect has been measured by comparing the results of the numerical model between the case with and without turbines under different flooding scenarios. Finally, the same methodology has been applied to a real case study selected from the previous group of estuaries namely the Solway Firth. An initial model has been created, according to the available data at the start of the research, which contained some errors related to the water depth at the intertidal areas in the upper estuary. Therefore, when a more realistic dataset became available, an improved model was created. The improved model has been used to assess the effects of tidal farms in the estuary under a coastal flooding event. It is concluded that there is significant influence of the channel geometry over the locations where the maximum changes in water levels due to the tidal farms will happen. Nevertheless, the effects seem to be more relevant in terms of the decrease rather than the increase of peak water levels for all geometries and the maximum changes seem to be in the order of dm. This is in agreement with the results of the Solway Firth models and can be summarised as a positive net effect over flood risk. On the other hand, a concern has been raised about the impact on intertidal areas, which could be the subject of future research.

The spatial and temporal (seasonal and diel) patterns in the hyperbenthic community structure (>500 μm) was investigated in the warm temperate, permanently open Kariega Estuary situated along the south-eastern coastline of South Africa. Spatial and seasonal patterns in the hyperbenthic community structure were assessed monthly at six stations along the length of the estuary over a period of twelve months. Data were collected using a modified hyperbenthic sledge, comprising two super-imposed nets. Physico-chemical data indicate the presence of a constant reverse salinity gradient, with highest salinities measured in the upper reaches and lowest at the mouth of the estuary. Strong seasonal patterns in temperature, dissolved oxygen and total chlorophyll-a (chl-a) concentrations were evident. Total average hyperbenthic densities ranged between 0.04 and 166 ind.m-3 in the lower net and between 0.12 and 225 ind.m-3 in the upper net. Hyperbenthic biomass values ranged between 0.02 and 11.9 mg.dry weight.m-3 in the lower net and between 0.02 and 17.4 mg.dry weight.m-3 in the upper net. A spatial and temporal pattern in total densities was detected with an increase in abundance over the period of September to October 2008 particularly in the middle reaches (Stations 3 and 4). Both the lower and upper nets were numerically dominated by decapods (mainly brachyuran crab zoeae) with the exception of June and July 2008 when mysids (mainly Mesopodopsis wooldridgei) dominated, making up 72.4 ± 58.14% of the total abundance in the lower net. A redundancy analysis (RDA) indicated that 99.2% of the variance in the hyperbenthic community structure could be explained by the first two canonical axes. Axis one, which accounted for 96.8% of the total variation detected in the ordination plot was highly correlated with sedimentary organic content and to a lesser extent the chl-a concentration within the Kariega Estuary. The correlations with the second canonical axis (2.4%) were less obvious, however, salinity and seston concentration were weakly correlated with this axis. Diel variability in the hyperbenthic community structure was assessed during March 2009. Samples were collected during the day and night (n = 6 for each period) using sampling gear described above. Total average hyperbenthic densities during the day (497.9 ± 254.1 ind.m-3) were significantly higher than night-time estimates (129.9 ± 38.5 ind.m-3; p<0.05). There were no significant differences in the average dayand night-time estimates of hyperbenthic biomass (p>0.05). A hierarchical cluster analysis identified two significantly distinct groupings, designated the day and night samples. Results from the SIMPER procedure indicated that the high densities of crab zoeae recorded during the day-time accounted for the majority of the dissimilarity between the day and night groupings (44.7%). In addition, it is apparent that several benthic species, especially from the cumacean and isopod orders, were absent from the hyperbenthos during the day-time and emerged into the water column at night.

Temporarily open/closed estuaries (TOCEs) are the dominant estuary type in South Africa. These systems are often characterized by extensive beds of submerged macrophytes, which form important foraging and shelter habitats for fishes, especially for estuary-dependent fish species such as the Cape stumpnose Rhabdosargus holubi and Cape moony Monodactylus falciformis that are commonly associated with them. A loss of submerged macrophytes from an estuary has been shown to affect the fish community as well as reducing overall system productivity. The TOC East Kleinemonde Estuary, situated in the warm-temperate biogeographic region of South Africa has been subject to an ongoing long-term fish monitoring project since 1995. During the period 1995 to 2002, this estuary contained large beds of the submerged macrophytes Ruppia cirrhosa and Potamogeton pectinatus. However, subsequent to a major flood event in 2003 these macrophytes have been largely absent from this system. The effect of the loss of submerged macrophytes on the East Kleinemonde fish assemblage was investigated through an analysis of seine and gill net catch data. Seine net catches for a 12 year period, encompassing six years of macrophyte presence and six years of macrophyte senescence, revealed changes in the relative abundance of certain fish species. Vegetation-associated species such as R. holubi and M. falciformis decreased in abundance whereas sediment-associated species, especially members of the family Mugilidae, increased in abundance following loss of the macrophytes in this estuary. The critically endangered pipefish Syngnathus watermeyeri was only recorded in catches during years in which macrophyte beds were present. In addition to the analysis of catch data, the importance of macrophytes as a primary energy source for selected estuarine fishes was explored through the analysis of carbon and nitrogen stable isotopes. Prior to conducting these analyses, common methodological practices to address the presence of carbonates and lipids within isotope samples were evaluated. A subset of samples were either acid washed to remove carbonates, or lipids were removed according to the method of Bligh and Dyer (1959) as both of these compounds have been shown to affect stable carbon and nitrogen isotope ratios. The suitability of the lipid normalization models of Fry (2002) and Post et al. (2007) for samples of estuarine fish muscle were also tested. Based on this evaluation both models are suitable for use with estuarine fish muscle tissue, however since neither carbonate nor lipid content of any of the samples used in this study was high all samples were left untreated in the following analysis. Carbon isotope ratios from a wide range of fish species collected from the East Kleinemonde Estuary during the macrophyte-senescent phase were compared with individuals of the same species from the neighbouring West Kleinemonde Estuary (where extensive beds of R. cirrhosa and P. pectinatus were present) and revealed the influence of submerged macrophyte material in the diet of fishes in the latter system. However, it was apparent that these plants are not directly consumed but rather contribute to the detrital pool that forms a food source for most invertebrate and some fish species. The most significant source of carbon for East Kleinemonde fishes during the macrophyte senescent phase appeared to have a more depleted origin; probably from benthic or pelagic microalgae. In conclusion, while the importance of macrophyte beds as shelter and foraging habitats for estuarine fishes are well documented, their role in terms of the structuring and functioning of fish assemblages in TOCEs remains somewhat uncertain. The findings of this study were possibly masked by the resilience of vegetation-associated species to the loss of this habitat, as well as by life history characteristics of species such as R. holubi that allow their numerical dominance despite habitat change. Nonetheless, macrophyte senescence in the East Kleinemonde Estuary resulted in the loss of at least one species and the reduced abundance of vegetation-associated species, probably reflective of reduced food resources and/or increased vulnerability to predation. As a result, beds of submerged macrophytes are an important habitat within TOCEs.

Introduction. On the coast of the Bathurst Division, Eastern Cape Province, some 42 miles south- east of Grahamstown, lie two estuaries opening within a few yards of one another. These are known as the Kleinemonde. This paper embodies the results of an ecological survey of the fauna of the Larger Kleinmond Estuary. The chief concern has been the aquatic fauna but animals living along the banks have also been dealt with as far as possible, as have the birds. This is the second of South Africa's blind estuaries to be studied, the Klein River, Hermanus, having been investigated in 1947-49 by workers from the University of Cape Town. Though common in South Africa, blind estuaries are rare in parts of the world such as Europe, and present an almost entirely new field of research for the ecologist. It is hoped, therefore, that the investigation of the Kleinmond Estuary and a comparison with the results obtained from the Klein River, Hermanus, will add to our knowledge of this subject and be of service to future investigators. The history of the Kleimnond River is worthy of mention, for the lagoon was not always blind and shallow. The early maps of the 1820 settlers show the river as a navigable stream; sailing vessels anchored out at sea and sent lighters up what was then known as the Lydendoch River to collect fresh meat. The area at the mouth of the river was called Port Jessie. In these early days the region abounded with game. Hippo and elephant were common. the former inhabiting deep pools in the upper reaches of the river; pools which have since completely silted up. Constant ploughing of the slopes adjoining the river has caused vast amounts of soil to be washed by rains into the stream, and today what was within living memory a deep, clear stream is a shallow, blind and muddy river, a reflection of man's destructive methods of farming. These changes must have had a far-reaching effect on the fauna of the estuary; an effect about which we can, alas, only speculate. It is perhaps significant that though rich in numbers, the fauna is poor in species, tunicates and echinoderms being apparently entirely absent. The work involved in this survey was undertaken between September 1952 and November 1953, under the direction of Professor J . Omer-Cooper of Rhodes University, frequent and regular visits being made to the estuary. The entire month of February, 1953, was spent on the shore of the lagoon, so that animals could be observed over a continuous period. The starting date of the survey was particularly fortunate, as on September 28th, 1952, the river came down in flood and broke through the sand-bar separating the lagoon from the sea. The work thus began at a definite point in the estuarine cycle, the importance of which it is hoped to show.

INTRODUCTION: Estuaries have received considerable attention from biologists in southern Africa in the past thirty years. Professor J.H. Day and the Zoology Department of the University of Cape Town have undertaken ecological surveys of a large number of estuaries of a variety of types, laying the important groundwork for more detailed studies of single species or problems. Their studies showed that the fish populations of southern African estuaries consist mainly of marine species. Why and when these species enter or leave the estuaries and whether they grow or breed in them were not established. The only relatively detailed study of a fish in southern African estuaries was that of Talbot (1955) who investigated aspects of the growth, feeding and reproduction of Rhabdosargus globiceps (Cuvier). No quantitative information has been published on any of the fish species, perhaps due to the difficulty of obtaining such data; results such as population estimates, mortality rates and growth rates are particularly unreliable when the area being investigated is in direct contact with the sea. Studies relating to even a single species of fish are hampered by immigration and emigration between estuary and sea, which make the population continuously variable. The larger estuaries are also difficult to sample adequately, especially with regard to netting, except perhaps on a very large scale using expensive commercial equipment. Along the southern African coast many of the smaller estuaries with a limited catchment area are cut off from the sea for most of the year due to erratic rainfall and longshore drift of sand. These closed or 'blind' estuaries of south eastern Africa, of which there are at least thirty in the eastern Cape Province, provide excellent areas for studying estuarine fish populations which enter when the estuary is open to the sea, but become isolated once it closes. These fish which become cut off in closed estuaries are subject to a new series of conditions. They are exposed to the wider temperature and salinity fluctuations of an estuary and they are forced to utilise the food resources of the estuary. Additionally they may be subjected to predation from piscivorous birds, and those predatory fish which are also cut off in the estuary. These factors will affect the size, mortality, and growth rates of a fish population. Since the fish are isolated no recruitment from the sea can take place, and any increase in numbers would have to come from breeding within the estuary. It is inevitable that the most numerous species should receive attention first. Rhabdosargus holubi (Steindachner) (Synonomy : Sargus holubi Stndr, Austrosparus tricuspidens Smith, Rhabdosargus tricuspidens (Smith))(Plate 1) is one of the most abundant fish in the estuaries of the eastern Cape Province. According to Smith (1965) it is endemic to southern Africa, occurring from the Cape to Zululand, being most common between Mossel Bay and East London. It is largely replaced by Rhabdosargus sarba (Forskal) in the north of its range and by Rhabdosargus globiceps (Cuvier) in the south. Commonly called the 'flatty' or silver bream it is considered only as being of nuisance value by fishermen, seldom exceeding 30 cm in length. In this study the ecology of R.holubi was examined in relation to the closed West Kleinemond estuary which is a small 'blind' estuary typical of those found along the eastern Cape coast. The growth, mortality and population size of R.holubi in this estuary were investigated and linked where possible with predation and detailed studies into the tolerances, food, and feeding of the species. It was not known at the commencement of the study whether breeding could occur in estuaries, although Smith (1965) stated that R.holubi does breed in estuaries. During the course of the sampling programme in the West Kleinemond estuary, measurements of the growth and population structure of two other species of fish were recorded for comparison with R.holubi: the sparid Lithognathus lithognathus (Cuvier) (White Steenbras) and the piscivorous carangid Hypacanthas amia (L.) (Leervis or garrick). Laboratory studies on R.holubi were possible due to the fact that large numbers of this species could be caught in eastern Cape estuaries and transported to Grahamstown, where they could be maintained in captivity for up to four months in recirculating seawater aquaria. During the course of the study over 2000 fish were kept in captivity at different times for a variety of experiments. Another 2500 were preserved for gut analyses and lipid determinations, while over 5000 were captured, examined and released in the field.

Dissolved organic matter (DOM) is a complex mixture of organic compounds and represents the largest reservoirs of carbon (C) on earth. Particulate organic matter (POM) is another important carbon component in C cycling and controls a variety of biogeochemical processes. Estuaries, as important interfaces between land and ocean, play important roles in retaining and transforming such organic matter (OM) and serve as both sources and sinks of DOM and POM. There is a diverse array of both autochthonous and allochthonous OM sources in wetland/estuarine ecosystems. A comprehensive study on the sources, transformation and fate of OM in such ecosystems is essential in advancing our understanding of C cycling and better constraining the global C budget. In this work, DOM characteristics were investigated in different estuaries. Dissolved organic matter source strengths and dynamics were assessed in a seagrass-dominated subtropical estuarine lagoon. DOM dynamics controlled by hydrology and seagrass primary productivity were confirmed, and the primary source of DOM was quantified using the combination of excitation emission matrix fluorescence with parallel factor analysis (EEM-PARAFAC) and stable C isotope analysis. Seagrass can contribute up to 72% of the DOM in the study area. The spatial and temporal variation of DOM dynamics was also studied in a freshwated dominated estuary fringed with extensive salt marshes. The data showed that DOM was primarily derived from freshwater marshes and controlled by hydrology while salt marsh plants play a significant role in structuring the distribution patterns of DOM quality and quantity. The OM dynamics was also investigated in a mangrove-dominate estuary and a comparative study was conducted between the DOM and POM pools. The results revealed both similarity and dissimilarity in DOM and POM composition. The dynamics of both OM pools are largely uncoupled as a result of source differences. Fringe mangrove swamps are suggested to export similar amounts of DOM and POM and should be considered as an important source in coastal C budgets. Lastly, chemical characterizations were conducted on the featured fluorescence component in OM in an attempt to better understand the composition and origins of the specific PARAFAC component. The traditionally defined ‘protein-like’ fluorescence was found to contain both proteinaceous and phenolic compounds, suggesting that the application of this parameter as a proxy for amino acid content and bioavailability may be limited.

Many of the larger estuaries along the South African coastline are highly degraded due to human impacts, highlighting the need to conserve pristine systems. There are approximately 100 micro-estuaries along our coastline which have not yet been identified or classified and hence their ecological functioning is unknown. The aim of this study was to identify and classify micro-estuaries along the Eastern Cape coast. Specific objectives of the study were to: 1) map the distribution of estuarine habitats in a selection of micro-estuaries and assess changes over time; 2) measure the physico-chemical characteristics of the micro-estuaries in the field; 3) investigate the distribution of vegetation, identify macrophyte species and habitat integrity and classify the micro-estuaries sampled using abiotic and botanical characteristics. A total of 30 systems were identified of which 10 were selected for sampling which took place seasonally (2015-2016). A relationship between open water surface area and short term rainfall was only evident in Cunge, Sandy Bottom and Ichabana. In the field it was observed that after heavy rainfall, open water surface area increased and mouth breaching occurred. Salinity was the only water column characteristic that showed differences; salinity increased in response to over wash events. Sand was dominant in all systems and there were no differences in sediment characteristics. A total of 8 macroalgal species, 16 estuarine macrophyte species, six freshwater species and 72 riparian plant species were found. Seed bank reserves were low in all systems despite them being vegetated. Only abiotic characteristics were used for classification using Principle Components Analysis since there were no botanical differences between systems. Micro-estuaries were larger (> 0.03 ha), had a higher salinity (oligohaline, mesohaline and polyhaline conditions) and a constant water body. Micro-outlets were smaller (< 0.03 ha), had a lower salinity (oligohaline conditions were most common) and showed periods of drying. Five micro-estuaries (Shelbertsstroom, Kwesani, Cunge, Mtwendwe and Mtendwe) and five micro-outlets (Stromatolite, Sandy Bottom, Ichabana, Palm Tree and Black Rock) were classified. This study has shown that the micro-estuaries along the Eastern Cape coast are pristine (i.e. intact riparian zone and catchment), untouched and highly dynamic systems (i.e. great variation in water surface area and mouth condition) highlighting their need for protection.