Global ETD Search

1	Investigation on Cell-Cell Junctions by Inhibition of Na,K-ATPase Activity / Studie av cell till cell kontakter genom inhibering av Na,K-ATPas aktivitet Boström, Caroline January 2021 (has links) This thesis report investigates the effect on cellcell junction proteins when the Na,K-ATPase (NKA) is inhibited. The main goal is to develop an understanding of how the NKA activity regulates the cell junction proteins. The investigated proteins are the adherens junction protein ECadherin, and the tight junction proteins Occludin and Claudin7.The NKA is inhibited by introducing the cardiotonic steroid Ouabain to the cells. The treatment is tested for different time lapse and different concentrations. The results show that all proteins are down regulated when treated with high concentrations (500 nM) of Ouabain. ECadherin is up regulated when treated with lower concentrations (10 nM) of Ouabain while Claudin7 is down regulated at low levels. / Detta examensarbete undersöker effekten på cell-cell junctions när Na,K-ATPas (NKA) inhiberas. Målet med rapporten är att få en förståelse för hur NKA aktiviteten reglerar cell-cell junction proteinerna. Proteinerna som undersöks är adherens junction proteinet ECadherin, och tight junction proteinerna Claudin7 och Occludin. NKA inhiberas genom att cellerna behandlas med den kardiotoniska steroiden Ouabain. Behandlingen testas under olika tidsperioder och för olika koncentrationer. Resultaten visar att alla proteiner är nedreglerade när de behandlas med höga koncentrationer (500 nM) av Ouabain. ECadherin blir uppreglerad när det behandlas med lägre koncentrationer (10 nM) av Ouabain medan Claudin7 nedregleras vid låga nivåer. NaK-ATPase Ouabain Cell junctions ECadherin Occludin Claudin7 Western Blot Fluorescent Imaging Tight junctions Adherens Junctions Immunocytochemistry Ouabain NaK-ATPase ECadherin Occludin Claudin7 Western Blot Immunocytokemi Cellkontakter Fluorescernsmikroskopi Physical Sciences Fysik
2	Effekte von Calcitriol auf die renale Fibrogenese / Effects of calcitriol on the process of renal fibrogenesis Volland, Marcel 30 May 2012 (has links) No description available. 610 Medizin, Gesundheit MED 418 MED 419 Medicine Renale Fibrose Calcitriol Fibroblasten EMT Kollagen Typ I Fibronektin E-Cadherin Tk173 Tk188 HK-2 renal fibrosis calcitriol fibroblasts EMT collagen fibronectin Ecadherin Tk173 Tk188 HK-2 44.61 44.88 44.89

Search results

Investigation on Cell-Cell Junctions by Inhibition of Na,K-ATPase Activity / Studie av cell till cell kontakter genom inhibering av Na,K-ATPas aktivitet

Effekte von Calcitriol auf die renale Fibrogenese / Effects of calcitriol on the process of renal fibrogenesis