Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2018-04-24T20:39:20Z No. of bitstreams: 1 2014 - Maria Clara da Silva Negreiros Botelho.pdf: 2378053 bytes, checksum: bfc9cb9e12f70927d727da1fba44c017 (MD5) / Made available in DSpace on 2018-04-24T20:39:21Z (GMT). No. of bitstreams: 1 2014 - Maria Clara da Silva Negreiros Botelho.pdf: 2378053 bytes, checksum: bfc9cb9e12f70927d727da1fba44c017 (MD5) Previous issue date: 2014-02-26 / CNPq / FAPUR (UFRRJ) / The aim of this study was to evaluate the efficacy of a collar impregnated with deltamethrin and propoxur to control infestations R. sanguineus and Ctenocephalides felis felis in beagle dogs and to evaluate the safety of treatment by clinical, haematological and biochemical tests, and to assess the dosage of serum cholinesterase in dogs after the treatment with a carbamate. Twenty beagles dogs were selected, divided into two groups: control and treated with a collar containing a combination of deltamethrin 4g and propoxur 12g. Each animal was infested with 50 R. sanguineus ticks (25 males and 25 females) and 100 C. felis felis fleas adults (50 males and 50 females). For the assessment of efficacy, 48 hours after each infestation, the mechanical removal of the parasites was performed. Each animal was infested with fleas and ticks on days -7 (before treatment), -2, +5, +12, +19 and +26. After day 26, every 14 days infestations were performed until the day +208. After this experimental day, the animals were infested with fleas only every 7 days until the termination of the study on day +271. For haematological and biochemical evaluations, blood samples were collected from animals in both groups every seven days until day +14. This experimental day up to +266, samples were collected every 14 days. After removal of the collars (day 274), three collections were made on days: +275, +281 and +288 , to assess the possible occurrence of changes in serum levels of serum enzyme butyrylcholinesterase (BChE). The acaricides effectiveness of the test product was 100 % on days +7, +21 and +28. The efficacy values were above 90% until day 126. As from day +182, the efficacy reached values lower than 80%. Only after day +168, levels declined below 80%. Pulicide efficiencies of the test product was 100 % on days +21, +28, +42, +56, +98 and +112. The efficacy values were above 90% until day +266, reducing 89.48% on day 273. The animals showed no changes in clinical and laboratory examinations that could be associated with adverse effects from the use of the product tested. It is concluded that a collar containing a combination of deltamethrin 4g and propoxur 12g was effective in controlling R. sanguineus ticks for up to 126 days and control C. felis felis fleas for up to 266 days in addition to proving to be safe for use in dogs. The animals showed no clinical and laboratory changes that could be associated with adverse effects from the use of the product tested, thus demonstrating that the association deltamethrin and propoxur in an impregnated collar is safe for use in dogs. / O objetivo do presente estudo foi avaliar a efic?cia de uma coleira impregnada com deltametrina e propoxur no controle de infesta??es por Rhipicephalus sanguineus e Ctenocephalides felis felis em c?es da ra?a beagle e avaliar a seguran?a cl?nica do tratamento atrav?s de exames cl?nicos, hematol?gicos e bioqu?micos, al?m de avaliar a dosagem da colinesterase s?rica em c?es submetidos ao tratamento com um carbamato. Para isso, foram selecionados 20 animais da ra?a beagle em bom estado sanit?rio, distribu?dos em dois grupos: controle e tratado com uma coleira impregnada com 4g de deltametrina e 12g de propoxur. Cada animal foi infestado com 50 carrapatos R. sanguineus (25 machos e 25 f?meas) e 100 pulgas C. felis felis adultas (50 machos e 50 f?meas). Para a avalia??o da efic?cia, 48 horas ap?s cada infesta??o, foi realizada a remo??o mec?nica dos parasitos. Cada animal foi infestado com pulgas e carrapatos nos dias -7 (antes do tratamento), -2, +5, +12, +19 e +26. Ap?s o dia +26 foram realizadas infesta??es a cada 14 dias at? o dia +208. Ap?s este dia experimental, os animais foram infestados somente com pulgas a cada sete dias, at? o encerramento do estudo, no dia +271. Para avalia??o hematol?gica e bioqu?mica, amostras de sangue foram coletadas dos animais de ambos os grupos a cada sete dias at? o dia +14. Deste dia experimental at? o dia + 266, as coletas foram realizadas a cada 14 dias. Ap?s a retirada das coleiras (dia +274), foram realizadas mais tr?s coletas nos dias +275, +281 e +288, para avaliar a poss?vel ocorr?ncia de altera??es nos n?veis s?ricos da enzima butirilcolinesterase s?rica (BChE). A efic?cia carrapaticida do produto em teste foi de 100% nos dias +7, +21 e +28. Os valores de efic?cia foram superiores a 90% at? o dia+126. Apenas a partir do dia +182, a efic?cia atingiu valores inferiores a 80%. Apenas ap?s o dia +168 os n?veis declinaram abaixo dos 80%. A efic?cia pulicida do produto em teste foi de 100% nos dias +21, +28, +42, +56, +98 e +112. Os valores de efic?cia foram superiores a 90% at? o dia+266, reduzindo a 89,48% no dia +273. Os animais n?o apresentaram altera??es em exame cl?nico e laboratoriais que pudessem ser associados a efeitos adversos provenientes do uso do produto testado. Conclui-se que a coleira impregnada com 4g de deltametrina e 12g de propoxur foi eficaz no controle de carrapatos R. sanguineus por at? 126 dias e no controle de pulgas C. felis felis por at? 266 dias. Os animais n?o apresentaram altera??es cl?nicas e laboratoriais que pudessem ser associadas a efeitos adversos provenientes do uso do produto testado, demonstrando assim que a associa??o deltametrina e o propoxur em uma coleira impregnada ? segura para uso em c?es.