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Call number: LD2668 .T4 1986 C87 / Master of Science / Chemical Engineering

Ethylene and carbohydrate deprivation were investigated as possible causes of bud abortion in Lilium longiflorum Thunb. Silver thiosulfate (STS) was investigated as an inhibitor of ethylene-induced abortion. Fourteen days of 92.5% irradiance reduction increased bud abortion when plants were exposed to 2.07 mM ethephon. Percent bud abortion was 39% and 60% for plants grown in full irradiance and reduced irradiance, respectively. Ethephon resulted in 54% abortion, regardless of irradiance at 4.15 mM. A 70% irradiance reduction for 14 days did not increase bud abortion when plants were treated with ethephon. STS was applied to plants at visible bud +2 weeks followed by ethephon application 2 days later. Bud abortion was reduced from 69 to 13% with 2 mM STS; the STS x ethephon interaction was significant. STS inhibited ethephon-induced bud abortion when applied at visible bud, 4 weeks prior to ethephon application; However, STS application at flower bud initiation did not prevent/reduce ethephon-induced bud abortion.

The microorganisms colonizing plants can have a significant effect on host phenotype, mediating such processes as pathogen resistance, stress tolerance, nutrient acquisition, growth, and reproduction. Research regarding plant-microbe interactions has focused almost exclusively on vascular plants, and we know comparatively little about how bryophytes -- including mosses, liverworts, and hornworts -- are influenced by their microbiomes. Ceratodon purpureus is a dioecious, cosmopolitan moss species that exhibits sex-specific fungal communities, yet we do not know whether these microbes have a differential effect on the growth and physiology of male and female genotypes. Using a common-garden design, we reared ten axenic genotypes of C. purpureus in a controlled environmental chamber. Clonal C. purpureus replicates, with and without the addition of a microbial inoculation, were used to test the effect of a mixed microbial community on vegetative growth, sex expression, photosynthetic efficiency (Fv/Fm and ETR), and chlorophyll content (CFR) for male and female mosses. We found that microbes had a negative impact on the growth and photosynthesis efficiency of C. purpureus, and this effect varied among genotypes of C. purpureus for ETR and growth. Microbes also had a positive, sex-specific effect on chlorophyll content in C. purpureus, with males exhibiting lower CFR values in the absence of microbes. C. purpureus sex expression was marginally negatively affected by microbe addition, but gametangia production was low overall in our experiment. We also conducted preliminary surveys using direct counts from moss ramets to assess the community composition of epiphytic algae associated with our microbe addition and control C. purpureus. These surveys identified three algal morphospecies in association with the microbe addition C. purpureus genotypes, as well as cyanobacteria, nematodes, rotifers, and testate amoeba. No algae, cyanobacteria, or micro-fauna were observed in the control plants. Transplantation of a mixed microbial community from field-to-laboratory conditions may be applied to other bryophyte species under varying environmental conditions to provide insight into how these diminutive yet important ecosystems will respond to environmental perturbation.

Oxidative stress is a natural consequence of photosynthetic oxygen evolution and redox enzyme processes. Trp oxidation to N-formylkynurenine (NFK) is a specific, reactive oxygen species (ROS)-mediated reaction. This thesis work describes the identification and functional characterization of NFK in oxygen evolving Photosystem II (PSII). Although proteomics studies have confirmed NFK modifications in many types of proteins, limited knowledge on the biochemical significance exists. In vitro studies in thylakoids and PSII membranes were used to establish a correlation between oxidative stress, NFK formation, and photoinhibition. The in vivo effect of preventing Trp oxidation to NFK was assessed by site-directed mutation in the cyanobacteria Synechocystis sp. PCC 6803. This work provides insight into the role of NFK in photosynthetic oxygen evolution and photoinhibition. Based on the current knowledge of NFK, ROS, and repair, a new model is described. In this modified model for photoinhibition and repair, NFK plays a role in signaling for turnover of damaged proteins. NFK may play a similar role in replacement of damaged proteins in other systems.

The lateral spatial separation between the circular polarization components of a linearly polarized light beam impinging at off-normal incidence on an air-semiconductor interface is investigated experimentally and theoretically. This fundamental optical phenomenon is referred to as the Spin Hall effect of light (SHEL). An optical pump-probe technique is demonstrated to resolve in situ the nanometer size SHEL displacement of a beam transmitted inside an absorptive material. Three different types of optical interactions in silicon and GaAs demonstrate the technique’s general applicability. First, resonant ∼150 fs pump and probe pulses at λ = 820 nm resolve the SHEL displacement via free-carrier absorption in a 10 μm thick silicon sample. The measured SHEL displacements for a p-polarized probe beam are obtained between −10 to 150 nm as a function of the angle of incidence on the sample. Different angles of incidence are achieved by keeping a fixed angular separation between the pump and the probe beams while rotating the sample about the axis perpendicular to the plane of incidence. In another experiment, an optically thin (500 nm thick) GaAs sample allows one to use Pauli-blocking as an optical interaction to investigate the polarization and angular dependence of the SHEL in the probe beam. For such a polarization-dependent imaging technique, the SHEL displacement in the pump beam also contributes to the measured signal and is evaluated experimentally. A probe beam at normal incidence is used to measure a SHEL displacement of ∼180 nm in a transmitted p-polarized pump beam impinging on the sample with an angle of incidence of 55 degrees. Finally, two-photon absorption is used to resolve the SHEL in a (001) oriented 500 μm thick GaAs wafer using an optical source generating sub-bandgap radiation (λ = 1550 nm) with a pulse duration of 120 fs. Linearly p- and s- co-polarized pump and probe beams are also used to investigate the polarization dependence of the SHEL. All the experimental results obtained using these different optical interactions agree with the theory within the experimental error. Finally, analytical expressions of the shifts experienced by the circular components of a beam impinging at an interface between two optical media are also derived for an incident beam with an arbitrary spatial distribution.

AbstractSome morphological and physiological changes resulting from prolonged plant growth under plastic screening materials (Lifelite) now being marketed in this country are reported.Lifelite filtered out all light wavelengths between 500 and 580 manometers, lowered the transmission level to 26 percent in the 380 to 500 nanometer range and transmitted up to 62 percent of the wavelengths in the 580 to 700 nanometer range.Lifelite enhanced the germination of spinach seeds, inhibited the germination of lettuce and tomato seeds and had no effect on the germination of cabbage or onion seeds.The only positive morphological change elicited by Lifelite was a considerable increase in stem elongation because of an enlargement of cells. All plants., except cabbage, grown under Lifelite showed a substantial decrease in pigmentation.Ball State UniversityMuncie, IN 47306

The lateral spatial separation between the circular polarization components of a linearly polarized light beam impinging at off-normal incidence on an air-semiconductor interface is investigated experimentally and theoretically. This fundamental optical phenomenon is referred to as the Spin Hall effect of light (SHEL). An optical pump-probe technique is demonstrated to resolve in situ the nanometer size SHEL displacement of a beam transmitted inside an absorptive material. Three different types of optical interactions in silicon and GaAs demonstrate the technique’s general applicability. First, resonant ∼150 fs pump and probe pulses at λ = 820 nm resolve the SHEL displacement via free-carrier absorption in a 10 μm thick silicon sample. The measured SHEL displacements for a p-polarized probe beam are obtained between −10 to 150 nm as a function of the angle of incidence on the sample. Different angles of incidence are achieved by keeping a fixed angular separation between the pump and the probe beams while rotating the sample about the axis perpendicular to the plane of incidence. In another experiment, an optically thin (500 nm thick) GaAs sample allows one to use Pauli-blocking as an optical interaction to investigate the polarization and angular dependence of the SHEL in the probe beam. For such a polarization-dependent imaging technique, the SHEL displacement in the pump beam also contributes to the measured signal and is evaluated experimentally. A probe beam at normal incidence is used to measure a SHEL displacement of ∼180 nm in a transmitted p-polarized pump beam impinging on the sample with an angle of incidence of 55 degrees. Finally, two-photon absorption is used to resolve the SHEL in a (001) oriented 500 μm thick GaAs wafer using an optical source generating sub-bandgap radiation (λ = 1550 nm) with a pulse duration of 120 fs. Linearly p- and s- co-polarized pump and probe beams are also used to investigate the polarization dependence of the SHEL. All the experimental results obtained using these different optical interactions agree with the theory within the experimental error. Finally, analytical expressions of the shifts experienced by the circular components of a beam impinging at an interface between two optical media are also derived for an incident beam with an arbitrary spatial distribution.

Mimusops caffra, Euclea netetensis, Olea woodiana and Peddiea africana are tree species associated with different successional stages in a coastal dune forest. Saplings of these tree species were established in four different light intensities. These were full sun, 40% shade, 70% shade and 90% shade. The hypothesis proposed that the species from different successional stages are preadapted for a particular light environment and are disadvantaged in other light environments. Growth, morphological, biochemical and physiological aspects of the four species in the four light environments were determined. Growth rates showed no consistent pattern with respect to light intensity. However, most species, irrespective of their successional status, had the best growth response in either 40% or 70% shade treatments. All the species showed typical sun and shade responses for morphological, anatomical and some biochemical characteristics. Photosynthetic responses were complex and showed no relationship between the successional status of the species and the light conditions in which they were grown. There appears to be very little relationship between the growth responses, the measured biochemical and morphological aspects, assimilation rates and the successional status of the species. Light intensity is therefore not the sole driving force of forest succession but one of the many factors that contribute to the overall process. / Thesis - (M.Sc.)-University of Natal, Durban, 1992.

Primary production at a single station in Kaneohe Bay, Oahu, Hawaii was studied over a six-month period. Vertical profiles of production, plant biomass, light, and temperature were obtained and the data applied to a production model. The diel changes in surface production were measured and used to estimate daily production. Primary production per unit surface area was found to average 1.5 grams carbon per square meter per day and was higher on days with little vertical stratification and with lower incident radiation. Light appeared to limit production below .12 langleys per minute which occurred below about five meters depth. / Typescript. Bibliography: leaves 39-41.