Ultraviolet light (UV) and hydrogen peroxide (H2O2) decrease eggshell bacteria. However, when combined, the optimum amount of each and effects on hatchability are unknown. In Experiment 1, when compared to other concentrations of H2O2 and lengths of UV, the combination of 1.5% H2O2 and 8 minutes of UV yielded optimum results with a 3 log10 CFU/egg reduction in bacteria on the eggshell. In Experiment 2, exposing eggs to this optimum combination yielded a 1000 fold reduction in eggshell bacteria but only a numerical increase in hatch of set and hatch of fertile. In Experiment 3, eggs exposed to repetitive treatments of H2O2 and UV yielded a 4 log reduction in eggshell bacteria but no differences in hatchability or chick characteristics. In conclusion, the combination of H2O2 and UV proved to be effective for eggshell sanitization, especially when used repetitively, and did not alter hatchability.