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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
71

Inactivation of Escherichia coli O157:H7 and Salmonella enteritidis in liquid egg products using pulsed electric field

Amiali, Malek January 2005 (has links)
No description available.
72

Evaluation of lactic acid bacteria for the acceleration of cheese ripening using pulsed electric fields

Briggs, Stephanie Sheryl January 2003 (has links)
No description available.
73

Effect of pulsed electric fields on physical properties of apples and potatoes

Arévalo, Patricio January 2003 (has links)
No description available.
74

Proteins in High Electric Fields

Landry, David Michael 01 December 2013 (has links) (PDF)
Being able to control protein function directly and in real time is attractive. In this thesis, I discuss controlling protein activity using alternating current electric fields on the order of 1 MV/m. Since protein structures result in localized and/or protein-wide charge gradients, it is expected that a sufficiently high electric field applied across a protein should result in structural distortions which can temporarily alter or halt protein function. The field is set to oscillate above the influences of the electrochemical double layer effects (1 kHz) and below the level needed for hydrodynamic rotation of proteins (10 MHz). A device is used to pass this field across a small volume of sample while allowing the solution to still be observed. Through application of high electric fields, we are able to temporarily reduce the activity of a bioluminescent luciferase reaction. Activity is inferred by measurement of the intensity and wavelength of the light emitted by the luciferase reaction. As this process is explored further, it could lead to the ability to electrically control protein function.
75

Effects of an Electric Field on Water Uptake in Single Roots of Intact Onion Plants

Sardarabadi, Bahram M. (Bahram Moory) 08 1900 (has links)
Water uptake by a single root of an onion plant (Allium cepa) was measured potentially before, during and following exposure of shoots to an external electric field (EEF). The field strength used was 9kV/m DC and AC (60-Hz) brought about a statistically significant increase (44-71%) in water uptake if the shoot chamber was at almost 100% humidity.
76

Cascaded High Voltage Converter with Variable Control for Pulsed Electric Field Applications

Loza, Emmanuel 01 June 2012 (has links) (PDF)
Living a sustainable lifestyle while facing increasing population and decreasing natural resources has become one of humanity’s largest challenges. Locating fossil fuels is becoming more difficult while the demand for them to power our societies is ever increasing. Instead of finding more efficient methods of extracting fossil fuels, developing technologies that create renewable substitutes for fossil fuels is now the strategy. Algae biofuel matches fossil fuel performance while also meeting the criteria for renewable energy. The focus now shifts to finding methods for commercially producing algae biofuel. Therefore, the objective of this thesis is to develop a system that provides the flexibility in finding the optimum operating conditions for lysing algae. Lysing is the process of disrupting the cell membrane in order to isolate the cellular components necessary to produce biofuel. The proposed system consists of cascaded power converters that provide a pulse output voltage in order to create a pulsed electric field (PEF) to lyse algae. The proposed system is unique from any known PEF systems because it provides the ability to independently adjust peak voltage, pulse width and frequency of the output voltage. This in turn provides great flexibility in determining optimum pulse voltage at various operating conditions for lysing algae. The system was tested on its ability to control the required variables while maintaining independence from the other variables. The new network was also designed and tested on how well it regulated the specific output waveform under the effects of different load currents as well as variations in the input voltage.
77

THE EFFECT OF ELECTRIC FIELDS ON MACROVOID PORES IN POLYMERIC MEMBRANES

RAY CHAUDHURI, SILADITYA 04 September 2003 (has links)
No description available.
78

Suppression of Thermoacoustic Instabilities Using An Electric Field and Feedback Control

Dustin L Cruise (13130481) 21 July 2022 (has links)
<p>In this work, a mechanism is developed for an electric field to modulate the heat-release of a laminar flame. This mechanism is then used with a feedback controller to suppress thermoacoustic instabilities in a Rijke tube.</p>
79

Investigation of Single-Cell and Blood-Brain Barrier Mechanics after Electroporation and in Primary Brain Cancers

Graybill, Philip Melvin 31 August 2021 (has links)
Cell-level and tissue-level mechanical properties are key to healthy biological functions, and many diseases and disorder arise or progress due to altered cell and tissue mechanics. Pulse electric field (PEFs), which employ intense external electric fields to cause electroporation, a phenomenon characterized by increased cell membrane permeability, also can cause significant changes to cell and tissue mechanics. Here, we investigate the mechanics of brain and brain cancer cells, specifically focusing on how PEFs impact cell mechanics and PEF-induced blood-brain barrier disruption. In our first study, we investigate single-cell mechanical disruption of glioblastoma cells after reversible electroporation using Nanonet Force Microscopy (NFM). A precise network of extracellular-matrix mimicking nanofibers enabled cell attachment and contraction, resulting in measurable fiber deflections. Cell contractile forces were shown to be temporarily disrupted after reversible electroporation, in an orientation and field-dependent manner. Furthermore, we found that cell response is often a multi-stage process involving a cell-rounding stage, biphasic stage, and a cell re-spreading stage. Additionally, cell viability post-PEFs was orientation-dependent. In another study, we investigated the mechanical properties of brain cancer for various-grade glioma cells (healthy astrocytes, grade II, grade III, and grade IV (glioblastoma) cells). A microfluidic constriction channel caused cell deformation as cells, driven by hydrostatic pressure, entered a narrow constriction. Finite element models of cell deformation and a neural network were used to convert experimental results (cell entry time and cell elongation within the channel) into elastic modulus values (kPa). We found that the that low-grade glioma cells showed higher stiffnesses compared to healthy and grade IV glioma cells, which both showed similar values. These results warrant future studies to investigate these trends further. PEFs can induce Blood-brain barrier (BBB) disruption, an effect we studied using a multiplexed, PDMS microdevice. A monolayer of human cerebral endothelial cells on a semi-permeable membrane was used to model the BBB, and permeability was assessed by the diffusion of a fluorescent dye from an upper to lower channel. A custom tapered channel and branching channel design created a linear gradient in the electric field within the device that enabled six electric field strengths to be tested at once against two unexposed (control) channels. Normalization of permeability by the control channels significantly removed experimental noise. We found that after high-frequency bipolar irreversible electroporation (HFIRE) electric pulses, permeability transiently increased within the first hour after electroporation, in a voltage- and pulse-number dependent manner. However, we found significant electrofusion events after pulsing at high voltages, which reduced monolayer permeability below baseline values. This device enables efficient exploration of a wide range of electroporation parameters to identify the optimal conditions for blood-brain barrier disruption. In another blood-brain barrier study, we incorporate dense, polystyrene nanofiber networks to create ultra-thin, ultra-porous basement-membrane-mimics for In vitro blood-brain barrier models. Fiber networks are fabricated using the non-electrospinning Spinneret-based Tunable Engineered Parameters (STEP) technique. Endothelial cells cultured on one side of the fiber network are in close contact with supporting cell types (pericytes) cultured on the backside of the fibers. Contact-orientation co-cultures have been shown to increase blood-brain barrier integrity, and our nanofiber networks increase the physiological realism of basement-membrane mimics for improve modeling. Finally, we investigate how cell viability post-electroporation is impacted by cell morphology. The impact of cell morphology (shape and cytoskeletal structure) on cell survival after electroporation is not well understood. Linking specific morphological characteristics with cell susceptibility to electroporation will enhance fundamental knowledge and will be widely useful for improving electroporation techniques where cell viability is desirable (gene transfection, electrofusion, electrochemotherapy) or where cell viability is undesirable (tumor ablation, cardiac ablation). Precise control of cell shape and orientation enabled by nanofiber scaffolds provides a convenient and expedient platform for investigating a wide variety of factors (morphological and experimental) on cell viability. Altogether, these investigations shed new light on cell mechanical changes due to disease and pulsed electric fields, and suggest opportunities for improving brain cancer therapies. / Doctor of Philosophy / In biology, structure and function are interrelated. Cells and tissue have structures that enable them to perform their proper function. In the case of disease, cell and tissue properties are altered, leading to dysfunction. Alternatively, healthy structures sometime hinder effective treatments, and therefore can be therapeutically disrupted to improve treatments. In this study, we investigate single-cell and multi-cellular mechanical change due to disease or after pulsed electric fields (PEFs), with a specific focus on the brain. Pulsed electric fields (PEFs) use electrodes to deliver short, intense pulses of electrical energy to disrupt cell membranes and change cell mechanics. We studied as single-cell contractility, cancer cell stiffness, and blood-brain barrier (BBB) disruption by PEFs. We found that PEFs cause significant change to cell shape and mechanics, and can disrupt the BBB. By studying several grades of brain cancers, we found that low-grade brain cancer (gliomas) showed increased stiffness compared to healthy and highly diseased (grade IV) cells. To mimic the BBB, we used microfluidic devices to grow specialized brain cells (endothelial cells) on permeable membranes and nanofibers networks and showed that these devices can mimic structures found in animals/humans. Finally, we studied how cell properties (such as shape) determine whether cells will survive PEFs. Taken together, our investigations improve the understanding of brain mechanics during disease and after PEFs, and suggest the usefulness of PEFs for improved brain cancer therapies.
80

Quantitative In Vitro Characterization of Membrane Permeability for Electroporated Mammalian Cells

Sweeney, Daniel C. 16 April 2018 (has links)
Electroporation-based treatments are motivated by the response of biological membranes to high- intensity pulsed electric fields. These fields rearrange the membrane structure to enhance the membrane's diffusive permeability, or the degree to which a membrane allows molecules to diffuse through it, is impacted by the structure, composition, and environment in which the cell resides. Tracer molecules have been developed that are unable to pass through intact cell membranes yet enter permeabilized cells. This dissertation investigates the hypothesis that the flow of such molecules may be used to quantify the effects of the electrical stimulus and environmental conditions leading to membrane electroporation. Specifically, a series of electrical pulses that alternates between positive and negative pulses permeabilizes cells more symmetrically than a longer pulse with the same total on-time. However, the magnitude of this symmetric entry decreases for the shorter alternating pulses. Furthermore, a method for quantitatively measuring the permeability of the cell membrane was proposed and validated. From data near the electroporation threshold, the response of cells varies widely in the manner in which cells become permeabilized. This method is applied to study the transient cell membrane permeability induced by electroporation and is used to demonstrate that the cell membrane remains permeable beyond 30 min following treatment. To analyze these experimental findings in the context of physical mechanisms, computational models of molecular uptake were developed to simulate electroporation. The results of these simulations indicate that the cell's local environment during electroporation facilitates the degree of molecular uptake. We use these models to predict how manipulating both the environment of cells during electroporation affects the induced membrane permeability. These experimental and computational results provide evidence that supports the hypothesis of this dissertation and provide a foundation for future investigation and simulation of membrane electroporation. / PHD

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