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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Simulating the electric field mediated motion of ions and molecules in diverse matrices

Hickey, Joseph 01 June 2005 (has links)
Electroporation is a methodology for the introduction of drugs and genes into cells. This technique works by reducing the exclusionary nature of the cell membrane [125, 129, 186, 189]. Electroporation has successfully been used in electrochemotherapy and electrogenetherapy [57, 68, 86, 87, 110, 112, 131]. The two major components of electroporation are an induced transmembrane potential and the motion of the deliverable through a compromised cell membrane into the target cell [38, 55, 62, 114, 131]. These two components are both dependent on the electrophoretic motion of charged species in an applied electric field [45, 64, 75, 77, 177]. Currently, the methods outlined for understanding electroporation have been focused on either a phenomenological perspective, e.g. what works, or modeling the electric fieldstrength in certain regions [12, 56, 87, 129, 146, 204, 205]. While this information is necessary for the clinician and the laboratory scientist, it doesn't expand the understanding of how electric field mediated drug and gene delivery works or EFMDGD. To increase the understanding of EFMDGD, new models are required that predict the motion of ions and deliverables through tissues to target areas [75, 77]. This document examines the design and creation of an electric field mediated drug and gene delivery model, EFMDGDM. Two example scenarios, ionic motion in tissues and gel electrophoresis, are examined in depth using the EFMDGDM. The model requires tuning for each scenario but only utilizes experimental parameters and one tunable parameter that is computed from regressed experimental data. The EFMDGDM successfully describes the two examples. Future work will incorporate the EFMDGDM as the backbone of an electric field mediated drug and gene delivery modeling package, EFMDGDMP.

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