Bacillus anthracis secretes a toxin which consists of three proteins that is the cause of the anthrax disease symptoms leading to death. They are called the protective antigen (PA), edema factor (EF) and lethal factor (LF). The three proteins self-assemble into toxic complexes after PA binding to its receptors present on host cells. The toxin receptor complexes are then internalized and acidic endosomal pH triggers pore formation by PA and translocation of the LF and/or EF into the cytosol. In this study, we labeled PA with 2-fluorohistidine, an analog of histidine with a dramatically reduced side-chain pKa, in order to test the hypothesis that histidine protonation triggers the pH dependent change from a prepore to a pore. We have analyzed its functional properties. It can be cut by furin or trypsin and it can also bind with one of its receptor, the VWA domain of CMG2 and form a heptamer as wild type PA. However, the pore formation can be blocked by this labeled protein when bound to CMG2. Independent experiments show that 2F-His labeled PA can also block translocation. By modifying the protein with 2F-His, we show that histidine in PA and CMG2 does not play as a pH-sensitive trigger in the pore formation process. We provide hypotheses for these findings. / Thesis (M.S.)--Wichita State University, College of Liberal Arts and Sciences, Dept. of Chemistry. / "July 2006." / Includes bibliographic references (leaves 96-102).

EDM is widely accepted in tool and die industries for its versatility in machining hardened steels. Its ability to machine heat treated hardened steels eliminates the conventional method of heat treatment after machining which results in distortions. However one draw back of the EDM process, which makes its uses limited, is the high cost and time for the electrode manufacture. The die sinking EDM process employs complex shaped electrodes which require specialized machining operation and often results in high lead times. Solid free form fabrication is found to be an excellent alternative for the conventional all metal electrodes for EDM. Rapid prototyping of the electrode models and electroforming with copper is a fervently studied method of producing cost effective electrodes. However most of the studies have reported premature failure of the electroformed electrodes, limiting its commercial use on a large scale. This study attempts to analyze the failure mechanism of electroformed stereo-lithography electrodes by conducting a finite element analysis of the electrode model using LSDYNA- 970. It has been shown that high temperatures and coefficient of thermal expansion mismatch are the primary reasons for the premature failures experienced in these types of electrodes. The study also proposes some design modifications to improve the electrode performance during repeated thermal loading. The modified electrodes showed significantly improved performance in analysis studies. / Thesis (M.S.)--Wichita State University, College of Engineering, Dept. of Industrial and Manufacturing Engineering / "December 2005." / Includes bibliographic references (leaves 109--111)

Thesis (M.F.A.)--Wichita State University, College of Liberal Arts and Sciences, Dept. of English / Full text of this thesis is not available

Thesis (M.F.A.)--Wichita State University, College of Liberal Arts and Sciences, Dept. of English / Full text of this thesis is not available

Thesis (M.F.A.)--Wichita State University, College of Liberal Arts and Sciences, Dept. of English / Full text of this thesis is not available

Thesis (M.F.A.)--Wichita State University, College of Liberal Arts and Sciences, Dept. of English / Full text of this thesis is not available

Grievance -- Makers of wreckage -- The birth. / Thesis (M.F.A.)--Wichita State University, College of Liberal Arts and Sciences, Dept. of English / Full text of this thesis is not available

Thesis (M.F.A.)--Wichita State University, College of Liberal Arts and Sciences, Dept. of English / Full text of this thesis is not available

Performance relationships on various behaviorally tested modalities i.e., verbal, and visual-spatial, supported the possibility that lower-level, working memory measures might be predictive of higher-level, long-term memory performances. Experiment 1 examined these relationships as they related to modality and the type of task/test used (Visual-Spatial Working Memory Span vs. Long-Term Memory Ability Tests). Span was related to performance on a standardized test of reading comprehension but not to the ability to mentally rotate objects. Thus, Experiment 2 examined the Span Task, finding that a central executive-consuming secondary task reduced span, but incongruent loop interference did not. This supported the task as a measure of working memory, according to Alan Baddeley’s fractionated model, and its use in subsequent experiments. Experiment 3 explored higher-level self-reported memory processes for parking location. Five factors of parking memory: Internal and External Interference Susceptibility, Concern, Confidence, and Strategy, were derived. An episodic component likely underlay both working and long-term location memory processes. As such, Experiment 4 examined the difference between participant’s forced ordering and non-forced ordering of locations on the Span Task and as a function of low or high parking factor scores. Overall, inter-factor correlations, their directionality, and correlations with the Span Task logically supported the factors. Individual differences in External Interference and Concern interacted with ordering Span Task terminal and primacy locations, respectively. Lower and higher-level memory process relationships likely exist. / "May 2006." / Includes bibliographic references (leaves 95-101)

Thesis (M.S.)--University of North Carolina Wilmington, 2009. / Title from PDF title page (February 16, 2010) Includes bibliographical references (p. 66-67)