Mini-dissertation submitted in partial compliance with the requirements of the Master's Degree in Technology: Homoeopathy, Durban Institute of Technology, 2003. / The aim of this study was to establish the efficacy of Withania somnifera in tincture, 1X and 6X homoeopathic dilutions (in 62% v/v ethanol) as an antimicrobial agent against the in vitro growth of Bacillus cereus, Pseudomonas aeruginosa, Klebsiella pneumonia, Escherichia coli and Staphylococcus aureus, as compared to 62% v/v ethanol only. The disc diffusion method was employed. W somnifera is indigenous to southern Africa and its use is well established amongst the traditional healers for many varied complaints. Infusions, decoctions and tinctures of the fresh and dry whole root are used. (Gericke and Van Wyk, 2000:150.) For this study 20 plates of Mueller-Hinton agar were inoculated with each bacteria, resulting in a total of 100 plates. Four dry discs previously impregnated with the test substances and two antibiotic discs were equidistantly placed on each plate and incubated at 3rC. The vancomycin and gentamycin discs were included to account for plate-to-plate variations in the sensitivity of the bacteria to the antimicrobial substances. The plates were observed at 18, 24 and 48-hour intervals. ' Statistical analysis was performed using the Friedman test to compare test and control substances at each observation interval. The Mann-Whitney-U test was used to compare the mean inhibition zones between test and control substances / M

Mini-dissertation submitted in partial compliance with the requirements for the Master's Degree in Technology: Homoeopathy, Durban Institute of Technology, 2003. / The purpose of this study was to determine the efficacy of Alepidea amatymbica tincture and homoeopathic dilutions to the 1st and 6th decimal potency as compared to ethanol (negative control) in the in vitro inhibition of Escherichea coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus and Bacillus cereus in terms of the disc diffusion test. Vancomycin and gentamicin were included in the study as positive controls in order to account for plate-ta-plate variations in the sensitivity of the bacterial strains to antimicrobial substances. Antimicrobial activity was expressed as the ratio of the inhibition zone (mm) produced by the test substance and the inhibition zone (mm) produced by the two combined antibiotic discs. For this study 20 Mueller-Hinton agar plates were assigned to each bacterial species and were inoculated with their respective bacteria. Four dry filter paper discs and two antibiotic discs were placed equidistantly on each agar plate. The filter paper discs had been previously impregnated with one of the test substances or the negative control using a triple impregnation technique utilizing a micro-pipette. The plates were then incubated at 37\xB0 C. The diameters of the zones of inhibition were measured at 18 hours, 24 hours and 48 hours. Data was analysed by means of the Statistical Package for Social Sciences (SPSS). Statistical methods utilized were Friedmans' test, Mann-Whitney U test, and Kruskall - Wallis Non-Parametric Analysis of Variance by Rank test. / M

Los bosques del Sur del Ecuador tienen un alto valor para la conservación puesto que están muy amenazados pero también poseen altos niveles de biodiversidad y endemismo. Sin embargo, como el proceso de conservación es inherentemente espacial, la baja disponibilidad de información espacial acerca de los factores que promueven la vulnerabilidad de estos bosques y acerca de la distribución espacial de los objetivos de conservación ha provocado varios obstáculos para la conservación de los mismos. El objetivo principal de esta tesis fue generar información espacial acerca de las amenazas y los valores biológicos que ocurren en esta región para priorizar áreas de conservación. El segundo capítulo analizó los patrones de deforestación y fragmentación en la región desde 1976 usando fotografías aéreas e imágenes satelitales para identificar las áreas de bosque remanente, los frentes de deforestación, las tasas anuales de deforestación y las dinámicas de la composición y configuración del paisaje del Sur del Ecuador. Se registraron tasas anuales de deforestación de 0.75% (1976 – 1989) y 2.86% para dos periodos consecutivos. Se encontró también que el Sur del Ecuador está bajo un creciente proceso de fragmentación debido al incremento en el número de parches, el decremento en el tamaño promedio de los parches y el incremento en el aislamiento de los parches. Este estudió también contribuyó al mejor entendimiento de las dinámicas de cambio de los bosques tropicales. Se registró que la mayor superficie de bosque natural fue degradado o convertido a pastizales y que los principales frentes de deforestación están localizados en las zonas de bajas altitudes en los bosques siempreverdes premontanos. El tercer capítulo describe los patrones espaciales y temporales de la presión humana, debido a que este es uno de los principales factores que influencian la efectividad de las estrategias de conservación. Para esto se adaptó el Índice de Impacto Humano (HF) generado por Sanderson et al. (2002) y así evaluar los cambios espaciales en el HF durante 26 años a nivel de paisaje y de ecosistema. Esta información permitió identificar algunos “hotspots de cambio” y las áreas con menor influencia para de esta forma evaluar como los diferentes factores humanos contribuyen al HF y demostrar cuan efectiva ha sido el área protegida más importante de la región para reducir la presión humana al interior y exterior de sus límites. Los resultados muestran un notable incremento en los niveles de presión humana en el Sur del Ecuador y un decremento en el número de áreas sin influencia. Se identificó también que uno de los más importantes “hotspots de cambio” está localizado en la región occidental del área de estudio y en la cuenca baja del Río Zamora. Los tipos de vegetación con mayor influencia humana fueron los bosques secos estacionales y los matorrales en donde la densidad de la población fue el principal factor humano que contribuyó a los niveles de presión humana observados. Finalmente, se encontró que el Parque Nacional Podocarpus ha sido parcialmente efectivo para reducir la presión humana en su interior y exterior, puesto que los niveles de HF se incrementaron al interior del área protegida y en el área buffer pero fueron menores a los observados en las áreas circundantes. El cuarto capítulo analizó los patrones de diversidad alfa y beta de plantas endémicas para evaluar la congruencia entre ambos patrones e identificar áreas con prioridad para ser conservadas. Se encontró que el “hotspot” de diversidad alfa para las plantas endémicas está localizado en los Andes y que es poco congruente con las zonas que muestran los más altos niveles de diversidad beta, los cuales están concentrados mayormente en las estribaciones orientales y occidentales de los Andes y en las cordilleras costeras y amazónicas. También se encontró que aproximadamente 40% de la superficie con altos niveles de diversidad alfa y beta ha desaparecido debido a la deforestación y que solo el 30% remanente está bajo alguna categoría de conservación. De esta forma se proponen 12 áreas potenciales con alta prioridad para la conservación, las cuales mayormente esta localizadas en el Sur del Ecuador, para de esta forma mejorar la representatividad y complementariedad de la actual red de reservas. Finalmente, el quinto capítulo analiza los principales hallazgos de esta investigación remarcando las implicaciones para la conservación y sugiriendo áreas potenciales para ser conservadas con base a los niveles de presión humana, vegetación remanente y patrones de diversidad alfa y beta de plantas endémicas en la región Sur del Ecuador.

A research report submitted to the Faculty of Science, University of the Witwatersrand, Johannesburg, in partial fulfilment of the requirements for the Degree of Masters of Science by Coursework and Research Report. Johannesburg November 2017. / Mountains are complex environments owing to their varying topography and geographic range, and as a result are havens for a variety of eco-systems and biodiversity. Mountain systems around the globe are potentially transforming due to increasing pressure from human-driven climate change. The possible effects of these pressures and overall consequences of the changes are difficult to predict due to the complexity of mountain habitats. Previous studies have recorded increasing temperatures in mountain systems as one of the consequences of climatic change. As a consequence of this warming trend, plant species that typically grow in lower altitudes may migrate to higher altitudes as those habitats become suitable. There are many different effects this may have on the local eco-system, such as the possibility of the migrating species outcompeting the local species or even hybridization occurring, resulting in a new species. Regardless, the movement of species from low to high elevations will have a direct effect on plant community dynamics in the area. South Africa is experiencing warming temperatures and has experienced a reported increase in mean annual temperature by 0.96 ºC over the last five decades. This research aims to understand the implications of inter-annual temperature and precipitation trends in the alpine, upper and lower montane thermal zones in the Drakensberg on two endemic plant genera, Rhodohypoxis (Hypoxidaceae) and Glumicalyx (Scrophulariaceae). A thermal zone refers to a temperature gradient at a specific altitude in a mountain system. Temperature and precipitation data from 1994 to 2014 were collected from four weather stations: Sani Pass (2874 m), Shaleburn (1614 m) and Giants Castle (1759 m) in South Africa, and Mokhotlong (2209 m) in Lesotho. These sites represent three thermal zones; Sani Pass is in the alpine zone, Mokhotlong is in the upper montane zone, and Shaleburn and Giants Castle are in the lower montane zone. The objectives of this research are to analyse and compare the temperature and precipitation trends, inter-annual variability, and annual number of frost days at each data collection site from 1994 to 2014, as well as infer the potential impact these changes may have on the local endemic plant genera. Results show a more pronounced increase in temperature in the lower thermal zones and a larger decrease in precipitation in higher thermal zones. The lower montane zone experienced the highest increase in temperature of up to 0.6 ºC over two decades. The alpine zone showed the largest decrease in precipitation of on average 27.5 mm of rainfall per annum over 20 years, while the lower montane zone displayed the largest inter-annual variability in both temperature and precipitation variables. The upper montane zone had a larger decrease in frost days over the 20 year period relative to the lower montane zone. Interestingly this work showed an increased warming pattern in the lower thermal zones relative to upper zones, which contrasts with work in other mountain ecosystems. This warming may create larger intermediate regions which could encourage the movement of endemic flora into neighbouring thermal zones. Movement between thermal zones may increase hybridization within plant genera which could change the structure of the plant communities and possibly result in altered floral populations. Keywords: Climatic change, Drakensberg, endemic flora, thermal zones, temperature and precipitation trends, inter-annual variability / LG2018

Submitted by Angela Maria de Oliveira (amolivei@uepg.br) on 2018-07-24T18:20:09Z No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Fabiane Paulitsch.pdf: 1288582 bytes, checksum: c019c172d12983ecb197493b2d5df4d8 (MD5) / Made available in DSpace on 2018-07-24T18:20:09Z (GMT). No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Fabiane Paulitsch.pdf: 1288582 bytes, checksum: c019c172d12983ecb197493b2d5df4d8 (MD5) Previous issue date: 2017-02-22 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / Levantamentos florísticos realizados nos “Campos Gerais” (Paraná, Brasil) indicam que Fabaceae é uma das famílias com maior riqueza e abundância da região. Esses estudos também apontam a elevada diversidade e endemismo de Mimosa spp. Nosso estudo relata a diversidade de rizóbios isolados de nódulos radiculares de plantas nativas de Mimosa gymnas Barneby. Vinte e nove estirpes foram obtidas de M. gymnast nodulíferas em três áreas do Parque Estadual do Guartelá, uma das mais importantes unidades de conservação dos Campos Gerais; os solos dessas áreas foram caracterizados como arenosos, ácidos, pobre em nutrientes e matéria orgânica e com elevados teores de alumínio. A árvore filogenética construída com sequencias parciais do gene 16S rDNA agrupou as estirpes em um grande clado sendo que algumas estipes foram mais relacionados com a espécie Paraburkholderia nodosa enquanto outras foram mais relacionadas com P. bannensi. A análise de perfil genômico baseada em BOX-PCR revelou um elevado grau de variabilidade intraespecífica entre os isolados. Resultados baseados na filogenia de sequencias concatenadas dos genes recA-gyrB dividiram os isolados em dois grandes grupos. O clado II não agrupou nenhuma estirpe tipo e os isolados apresentaram uma identidade nucleotídica de no máximo 97% com P. nodosa. A filogenia do gene nodC agrupou todos os isolados em um grupo único com elevado suporte estatístico, não agrupando com a sequencia correspondente de nenhuma estirpe tipo do gênero Paraburkholderia. Nossos resultados reforçam a constatação de que rizóbios do gênero Paraburkholderia são os preferenciais simbiontes de Mimosa na América do Sul e a associação de estirpes nodulíferas do gênero com condições edáficas particulares. Ainda, os resultados das análises filogenéticas sugerem que os isolados agrupados no clado II na árvore filogenética dos genes recA-gyrB podem representar uma nova espécie de Paraburkholderia, reinterando a importância de estudos de diversidade com plantas leguminosas nativas e endêmicas. . / Floristic surveys performed in the “Campos Gerais” (Paraná, Brazil) indicate that Fabaceae is one of the most species-rich and abundant families. These studies also pointed out the high diversity and endemism of Mimosa spp. Our study report the diversity of rhizobia isolated from root nodules of native Mimosa gymnas Barneby. Twenty-nine strains were obtained from from nodulating M. gymnas in three areas of Guartelá State Park, one of the most important conservation unities of the “Campos Gerais”; soils of these areas were characterized as sandy, acid, poor in nutrients and organic matter and with high aluminum contents. The phylogenetic tree constructed with partial 16S rDNA sequences grouped the strains in a larger cluster and some strains were more related with Paraburkholderia nodosa, although some strains were more related to P. bannensi. The analysis of the genomic profile by BOX-PCR revelead a high degree of intraspecific variability among the isolates. Results based on phylogeny of concatened genes recA-gyrB divided the strains in two large clusters. The cluster II didn’t grouped with any type strain and the isolates showed a nucleotide identity of 16S rDNA of at most 97% with P. nodosa. The nodC phylogeny grouped all the strains into a well-supported clade, not grouping with any know strain of Paraburkholderia genus. Our results support that Paraburkholderia are the main symbionts of Mimosa in South America and the association of nodulating strains of this genera with particular edaphic conditions. Also, the results of the phylogenetic analysis suggest that the isolates grouped in cluster II in the recA-gyrB phylogenetic tree may represent a new species of Paraburkolderia, highlighting the importance of diversity studies with native and endemic leguminous plants.

Thesis (M.A. (African Languages)) --University of Limpopo, 2007 / Refer to the document

This thesis examines Greater Melbourne’s indigenous plants movement from the 1930s to the early twenty first century. It demonstrates the important scientific and educational role of the public intellectual, Professor John Turner, and of the Melbourne University Botany School which he led for thirty five years. The case study of the movement within the City of Sandringham and its successor the City of Bayside reveals how the inhabitants of an urbanised are responded to threats to the indigenous trees and wildflowers of their neighbourhood, stimulating botanists to assist them and using political means in order to achieve their conservation objectives. The thesis draws upon a range of local archives, conservation literature and private papers.

Conservation strategies are mainly focused on species existing in an environment shaped by natural and anthropogenic pressures. Yet, evidence shows that climate is changing faster than ever and expected to continue to change in the near future, which can be devastating for plants with restricted ranges. Turkey harbors many endemic species that might be affected from these changes. However, available data is scarce and biased, complicating the anticipation of future changes. Aim of this study is to improve our understanding of endemic species distributions and forecasting effects of climate change via species distribution modelling (SDM). The study is based on two Anatolian (Crocus ancyrensis and Crataegus tanacetifolia) and two Ankara (Salvia aytachii and Centaurea tchihatcheffii) endemics. Independent presence and absence data (ranging between 19-68 and 38-61, respectively) for each species was collected through fieldwork in and around the Upper Sakarya Basin in 2008 and 2009. With the software Maxent, SDMs were performed by using 8 least correlated environmental features and random presence records (of which 25% were used for confusion matrix). SDMs for current distributions of C. ancyrensis, C. tchihatcheffii and C. tanacetifolia were reliable enough for future extrapolations despite errors originating from scale, non-equilibrium status and biotic interactions, respectively. The model for S. aytachii failed due to absence of limiting factor (soil type) in the model. Future projections of those three species modelled using CCCMA-CGCM2 and HADCM3 climate models indicated three possible responses to climate change: (1) Extinction, especially for habitat specialists / (2) Range expansion, especially for generalist species / and (3) Range contradiction, especially for Euro-Siberian mountainous species. Species modelling can be used to understand possible responses of plant species to climate change in Turkey. Modelling techniques should to be improved, however, especially by integrating other parameters such as biotic interactions and through a better understanding of uncertainties.

Thesis (M.S.)--Oregon State University, 2010. / Printout. Includes bibliographical references (leaves 31-39). Also available on the World Wide Web.

Submitted in fulfillment of the requirements for the Degree of Master of Technology: Biotechnology, Durban University of Technology, 2009. / Dichrostachys cinerea, Carpobrotus dimidiatus, Capparis tomentosa and Leonotis leonurus are all plants that are indigenous to South Africa. These plants are used in traditional medicine to treat various ailments. However, there is little or no scientific data to justify these traditional uses. Furthermore, it is difficult to reconcile traditional knowledge with scientific evidence because of the overwhelming targeting of signal-responsive systems by plant defensive compounds, multiple sites of action and the connectedness of the signaling pathways, which provide many cures and have pleiotropic effects. In order to evaluate the action spectrum of these plants, and validate its widespread use, this research evaluated the antibacterial, antioxidant, anti-inflammatory, anti-mosquito and immunomodulatory properties of these plants. Antimicrobial activity of the extract was determined by evaluating the bactericidal and fungicidal action using the agar disc diffusion assay. Anti-oxidative properties of the extracts were tested using the DPPH photometric assay. Anti-inflammatory properties were carried out using the 5-lipoxygenase assay. The larvicidal, repellency and insecticidal assay was determined against A.arabiensis. The safe use of these plant extracts was determined by evaluating toxicity, a brine shrimp lethality assay and an in vitro cell culture system using human myelogenous leukemia cell line. Potential carcinogenic activity was evaluated using the Ames Salmonella Mutagenecity assay. The immunomodulatory activity of the extracts on human peripheral blood mononuclear cells 6 was evaluated on freshly harvested lymphocytes using the MTT assay. Cytokine response was evaluated by measuring the secretion of interferon-gamma and interleukin-10. Elucidation of the B cells, T cells, activated T cells, CD 4+, CD 8+ and NK cells was performed by flow cytometry. The extracts showed anti-microbial activity against Escherichia coli, Pseudomonas aeruginosa, Klebsiella oxytoca, Salmonella typhimurium, Serratia marcescens, Bacillus cereus and Tricoderm sp. The highest activity was shown by methanolic and aqueous extracts of L. leonurus leaves followed by methanolic and aqueous extracts of D. cinerea. Extracts of C. tomentosa and D.cinerea demonstrated a higher degree of free radical scavenging than rutin, which was used as a standard indicating that these plants have strong antioxidant properties. None of the plants showed significant anti-inflammatory activity when compared to NDGA. In the anti-mosquito assays, the extracts showed strong repellency and insecticidal activity. L. leonurus extracts demonstrated the highest insecticidal and repellency activity against the mosquito, and was also found to cause ‗knockdown‘ and mortality. The extracts display no toxicity, cytotoxicity and mutagenicity. The immunological studies for immune modulation showed that the methanol extracts of these plants induce a Th1- predominant immune response because they significantly suppressed the secretion of IL-10 and augment IFN-γ production, which are hallmarks used to indicate a stimulation of the innate immune response. This study also provides new information, with respect to the potential use of these plants in producing a mosquito repellent and an immunostimulant.