O objetivo do presente trabalho foi avaliar a resposta tecidual em 48 pré- molares (96 raízes) de cães, após obturação dos canais radiculares com diferentes materiais. Os dentes foram submetidos à biopulpectomia, ao preparo biomecânico e divididos em oito grupos, onde os canais radiculares foram obturados com os seguintes materiais: Grupos I e V pasta Calen® espessada com óxido de zinco; Grupos II e VI cimento EndoREZ; Grupos III e VII cimento Sealapex®, e Grupos IV e VIII cimento de óxido de zinco e eugenol. Decorridos os períodos de 30 e 90 dias, os animais foram mortos, as peças removidas e submetidas ao processamento histológico. A avaliação histopatológica foi baseada nos seguintes parâmetros: intensidade do infiltrado inflamatório, espessura do ligamento periodontal, reabsorção do tecido ósseo, limte de obturação e presença ou ausência de selamento apical. Os resultados foram submetidos à análise estatística pelo teste não paramétrico de Kruskal-Wallis, com nível de significância de 5%. Com base nos resultados obtidos e considerando as condições específicas deste trabalho, pôde-se concluir que a pasta Calen® espessada com óxido de zinco foi o material que apresentou a melhor resposta tecidual, seguida pelos cimentos Sealapex® e óxido de zinco e eugenol. O cimento EndoREZ apresentou resposta tecidual desfavorável . / The objective of the present work was evaluate the tissue response in 48 premolars( 96 roots), of dogs teeth, after pulpectomy, with different roots canals filling materials. The teeth were submitted to the biomechanical preparation and divided in eigth groups, where the canals were filled with following materials: Groups I and V - Calen® paste thickened with zinc oxide; Groups II and VI - EndoREZ sealer; Groups III and VII - Sealapex® sealer; and Groups IV and VIII Zinc oxide and eugenol cement. After periods of 30 and 90 days, the animals were killed, the pieces reduced and submitted to the histological processing. The histopathological evaluation was based on the following parameters: intensity of the inflammatory infiltrate, thickness of the periodontal ligament, bone resorption,filling limit and presence or absence of sealed apical. The results were submitted to the statistical analysis by the non parametric test of Kruskal-Wallis, with significance level was 5%. Based on the results obtained in histopathological analysis and methodologies employed in the present work, it may be conclued that: Calen® paste thickened with zinc oxide was the material that showed the better tissue response, following by Sealapex® sealer and zinc oxide and eugenol cement. EndoREZ sealer showed tissue response unfavorable.

O objetivo do presente trabalho foi avaliar a resposta tecidual em 48 pré- molares (96 raízes) de cães, após obturação dos canais radiculares com diferentes materiais. Os dentes foram submetidos à biopulpectomia, ao preparo biomecânico e divididos em oito grupos, onde os canais radiculares foram obturados com os seguintes materiais: Grupos I e V pasta Calen® espessada com óxido de zinco; Grupos II e VI cimento EndoREZ; Grupos III e VII cimento Sealapex®, e Grupos IV e VIII cimento de óxido de zinco e eugenol. Decorridos os períodos de 30 e 90 dias, os animais foram mortos, as peças removidas e submetidas ao processamento histológico. A avaliação histopatológica foi baseada nos seguintes parâmetros: intensidade do infiltrado inflamatório, espessura do ligamento periodontal, reabsorção do tecido ósseo, limte de obturação e presença ou ausência de selamento apical. Os resultados foram submetidos à análise estatística pelo teste não paramétrico de Kruskal-Wallis, com nível de significância de 5%. Com base nos resultados obtidos e considerando as condições específicas deste trabalho, pôde-se concluir que a pasta Calen® espessada com óxido de zinco foi o material que apresentou a melhor resposta tecidual, seguida pelos cimentos Sealapex® e óxido de zinco e eugenol. O cimento EndoREZ apresentou resposta tecidual desfavorável . / The objective of the present work was evaluate the tissue response in 48 premolars( 96 roots), of dogs teeth, after pulpectomy, with different roots canals filling materials. The teeth were submitted to the biomechanical preparation and divided in eigth groups, where the canals were filled with following materials: Groups I and V - Calen® paste thickened with zinc oxide; Groups II and VI - EndoREZ sealer; Groups III and VII - Sealapex® sealer; and Groups IV and VIII Zinc oxide and eugenol cement. After periods of 30 and 90 days, the animals were killed, the pieces reduced and submitted to the histological processing. The histopathological evaluation was based on the following parameters: intensity of the inflammatory infiltrate, thickness of the periodontal ligament, bone resorption,filling limit and presence or absence of sealed apical. The results were submitted to the statistical analysis by the non parametric test of Kruskal-Wallis, with significance level was 5%. Based on the results obtained in histopathological analysis and methodologies employed in the present work, it may be conclued that: Calen® paste thickened with zinc oxide was the material that showed the better tissue response, following by Sealapex® sealer and zinc oxide and eugenol cement. EndoREZ sealer showed tissue response unfavorable.

Magister Chirurgiae Dentium (MChD) / The goal of root canal treatment is to eradicate microorganisms in the root canal system of the tooth. However; it has been found that no method of tooth preparation is efficient in eliminating all microorganisms present in root canals. Therefore, obturation materials with anti-microbial properties are advantageous, so that any residual microorganisms in the root system of the tooth can be eliminated. Therefore, the aim of the study was to assess the antimicrobial effect of 3 endodontic sealers: Sealapex™, EndoREZ™ and Guttaflow bioseal™ against Enterococcus faecalis. The Direct Contact test was used to assess the antibacterial effect of the 3 sealers against E. Faecalis. Sample size was n=50 per sealer. The survival of bacteria was assessed by culturing aliquots of 100 μL onto Tryptic Soy Agar plates after 10-fold serial dilutions. After incubation for 24 hours at 37⁰C, colonies on the plates were counted, and the CFU/mL was calculated. The experiments were performed in triplicates. Testing after setting enabled the assessment of the antimicrobial activity of aged sealers after 7 days, 14 days, 21 days and 28 days. All 3 sealers displayed evidence of antibacterial activity against E. Faecalis with various degrees of antibacterial activity at day 0, 7, 14, 21 and 28. Antibacterial activity was displayed by all 3 sealers against E. Faecalis which will have an effect on entombed bacteria.