Commissioned for this report is Ramboll, Bridge and Tunnel Unit in Stockholm. The idea throughout the work has been to primarily help bridge designers compiling advice and instructions on how fatigue verification for railway bridges in reinforced concrete shall be performed under the new rules, the Eurocodes. After the introduction of the Eurocodes in 2011 has major problems emerged among designers around Sweden. In some areas, it differs in the calculation rules compared with rules from the Swedish Transport Administration and the National Board of Housing, Building and Planning calculation. Fatigue verification is one area where the new calculation rules are different from the past. A large part of the report has been to interpret and find the relevant information in the Eurocodes concerning fatigue. When questions or doubts have surfaced, this has been discussed with Elisabeth Helsing at the Swedish Transport Administration and bridge designers at Ramboll. The result is a report where the structure is organized in the way that the bridge designers can move from cover to cover and realize that fatigue verification in reality follows the same order as the report's sections. The issues have been resolved and reported regularly through the report with complementary findings as a result. Some of the greatest advances in the report are the load combinations to be used for the method for fatigue verification, how the dynamic factor and the magnification factor should be used and how the trainloads to be designed for each method for fatigue verification.