Circadian clocks are responsible for scheduling many behavioral and physiological processes to occur at the most appropriate time of day. The resulting daily rhythms also synchronize (entrain) to external environmental cues, known as zeitgebers. This phenomenon of entrainment enables organisms to anticipate daily changes in environmental conditions such as sunrise/sunset, temperature variations, availability of prey, etc. Given the critical nature of entrainment to survival, it is no surprise that the mechanism is conserved across taxa. The misalignment of the intrinsic clock with the external environment results in a plethora of negative consequences, made apparent by studies involving shift work and jet lag. The focus of the present study is to investigate the chronobiology of Larinioides cornutus (Araneidae), a nocturnal orb-weaving spider, with an emphasis on its entrainment to light:dark cycles. Because spiders have received scarce attention with respect to their chronobiology, it is instructive to compare the properties of spider circadian systems with those of the more established circadian model systems, such as Drosophilaand Murines. We found that both lights-off and lights-on are equally influential zeitgeber cues for (determines the phasing of) both activity onset and offset. Locomotor activity typically begins within a half hour after nightfall, continues throughout the night, and ceases just prior to dawn. Phase shifting experiments show that these spiders can re-entrain within 2 days to a light/dark cycle shifted by 6 hours, and within 3 days when shifted by 12 hours. These rates of re-entrainment occur at an extremely accelerated rate compared to mammals, which readjust to a time shift at a rate of around 1 day/ 1 hour of phase shift. In other words, spiders have a minimal jet-lag response. This suggests an increased level of plasticity in the spider circadian clock that has yet to be observed in other organisms. Typical of circadian rhythms in nearly all organisms, activity also persisted (free-runs) under constant conditions. However, in constant darkness (DD), a drastic change in periodicity was revealed in 66% of individuals, from 23.4 to 25.2. This particular phenomenon is rare and likely indicates the possible interaction of multiple oscillators. Further evidence to support this interpretation is the consistent periods of the rhythm displayed before and after the change. In contrast, under constant light (LL) conditions, 65% of spiders were arrhythmic, with 4 individuals ceasing activity completely. Significant periods detected in LL were normally distributed over an unusually broad range, from 16.7 to 34.9 hours, suggesting a high sensitivity to light. Because of the unusual rates of re-entrainment to light/dark cycles, the spontaneous changes in free-running period under DD, and arrhythmicity in LL, we propose that spiders are valuable comparative model organism for elucidating fundamental mechanisms of circadian clocks.

Most organisms maintain a nearly 24-hour circadian rhythm which allows them to anticipate daily events in the Earth’s solar day. Circadian clocks can be regulated by external time cues such as light/dark (LD) cycles, allowing nocturnal organisms to synchronize their rhythms around dusk in a process known as entrainment. In the absence of external time cues, these rhythms persist, revealing the period of the organism’s internal clock. In nature, day-night cycles have gradual transitions at dawn and dusk, yet these transitions often are not reflected in laboratory studies. Most utilize instantaneous transitions from light to dark, including previous work on spider circadian behavior. To mimic natural conditions, the next logical step is to evaluate how a gradual, dusk transition affects activity patterns and entrainment in nocturnal spiders. Two spider species were used: Pholcus phalangioides and Metazygia wittfeldae. Three hypotheses were identified: 1) spider circadian behavior is unaffected by the type of dusk transition; 2) spider activity entrains to a proportional decrease in light intensity; and 3) spider activity entrains to a specific dimness threshold. Spiders were placed in activity monitors and exposed to 12:12 LD cycles with high (8934 lux) and low (2918 lux) light intensities. There were two groups within each light intensity: one with instantaneous, dusk transitions and one with linear 4-hour transitions. In all experiments, spiders were switched to constant darkness after 5 days of LD cycles to identify the phasing of the circadian clock. In P. phalangioides, there were no significant differences among onsets of activity for high and low light intensities with ramped transitions, nor high and low intensities with instantaneous transitions. The average onset of activity was 0.2 ± 0.1 hours after absolute darkness. For M. wittfeldae, onsets of activity were significantly earlier in ramped than instantaneous LD cycles. However, there was no observable difference in activity onset associated with absolute light intensity for either transition type. The average onset of activity for ramped transitions was 1.9 ± 0.5 hours before complete darkness, while the average onset for instantaneous transitions was 0.5 ± 0.1 hours after complete darkness. Pholcus phalangioides requires complete darkness before activity begins, supporting the hypotheses that either the activity onset is unaffected by the type of dusk transition or requires a very low threshold of light. However, M. wittfeldae began activity about 2 hours earlier or around when light decreases by 50%, supporting the hypothesis that this species entrains to a proportional decrease in light intensity. These activity patterns match their ecologies, as P. phalangioides typically sits and waits in a permanent web while the orbweaver M. wittfeldae begins building a new web during the dusk transition. Overall, these results suggest that ramping light cycles should be considered when circadian rhythms are evaluated in the lab.

Air entraining admixtures (vinsol or non-vinsol) are used in concrete mixes that will be exposed to freeze-thaw cycles or sulfate attack. Entrained air is intended to improve the durability and resistance of hardened concrete to freeze-thaw cycles. However in many instances a significant loss of compressive strength has been observed in concrete mixes containing non-vinsol admixtures. Mixture forensic analysis has determined air void coalescing to be the main factor in observed low compressive strengths. The result of air void coalescing is the over design of compressive strength to account for the possible lower strengths and decreased confidence in the compressive strengths obtained during the quality control/assurance process.

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Even without formal training, adults can easily perceive, clap, tap, and move in time to a musical beat, but these behaviours are more difficult for children and the development of these abilities in childhood is not well understood. Until the present thesis, there were no developmentally appropriate tasks to separately assess musical beat perception and beat synchronization in children. In Chapter 2, I created a child friendly video judgment task to assess beat perception in the context of both simple and complex musical timing, and demonstrated that five-year-old children’s ability to perceive both tempo- and phase-driven beat misalignments is affected by metric complexity. In Chapter 3, I again used the complex Beat Alignment Task (cBAT) to show that the detection of beat misalignment is not significantly affected by the inclusion of dynamic video stimuli compared to static images. Chapter 4 expanded the perception task by adding a tapping synchronization component, and tested both five- and seven-year-old children. The complex Beat Alignment and Tapping Task (cBATT) showed that although children’s overall perceptual sensitivity improves with age, the perceptual bias for simple structures persists. However, although children were significantly better at tapping to metronomes than to songs, musical tapping synchronization was not obviously affected by metric complexity. Instead, performance related to other acoustic characteristics of the music, such as spectral flux, energy, and density. Together, these findings suggest that musically untrained children are sensitive to phase and tempo information in a perception-only task, and show perceptual specialization for culturally typical musical metres, but this is not the case for production. Thus, beat synchronization ability appears to be somewhat dissociated from beat perception in children. These studies represent the first use of a developmentally appropriate task to separately assess children’s beat perception and synchronization while also examining the role of metre and early experience. / Thesis / Doctor of Philosophy (PhD)

Humans must process fleeting auditory information in real time, such as speech and music. The amplitude modulation of the acoustic waveforms of speech and music is rhythmically organized in time, following, for example, the beats of music or the syllables of speech, and this property enables temporal prediction and proactive perceptual optimization. At the neural level, external rhythmic sensory input entrains internal neural oscillatory activities, including low-frequency (e.g., delta, 1-4 Hz) phase, high-frequency (e.g., beta, 15-25 Hz) power, and their phase-amplitude coupling. These neural entrainment activities represent internal temporal prediction and proactive perceptual optimization. The present thesis investigated two critical but previously unsolved questions. First, do these multiple entrainment mechanisms for tracking auditory rhythm have distinct but coordinated perceptual functions? Second, does regularity in the temporal (when) domain associate with prediction and perception in the orthogonal spectral (what) domain of audition? This thesis addressed these topics by combining electroencephalography (EEG), psychophysics, and statistical modeling approaches. Chapter II shows that beta power entrainment reflects both rhythmic temporal prediction (when events are expected) and violation of spectral information prediction (what events are expected). Chapter III further demonstrates that degree of beta power entrainment prior to a pitch change reflects how well an upcoming pitch change will be predicted. Chapter IV reveals that rhythmic organization of sensory input proactively facilitates pitch perception. Trial-by-trial behavioural-neural associations suggested that delta phase entrainment reflects temporal expectation, beta power entrainment reflects temporal attention, and their phase-amplitude coupling reflects the alignment of these two perceptual mechanisms and is associated with auditory-motor communication. Together, this thesis advanced our understanding of how neural entrainment mechanisms relate to perceptual functions for tracking auditory events in time, which are essential for perceiving speech and music. / Thesis / Doctor of Science (PhD) / Perceiving speech and musical sounds in real time is challenging, because they occur in rapid succession and each sound masks the previous one. Rhythmic timing regularities (e.g., musical beats, speech syllable onsets) may greatly aid in overcoming this challenge, because timing regularity enables the brain to make temporal predictions and, thereby, anticipatorily prepare for perceiving upcoming sounds. This thesis investigated the perceptual and neural mechanisms for tracking auditory rhythm and enhancing perception. Perceptually, rhythmic regularity in streams of tones facilitates pitch perception. Neurally, multiple neural oscillatory activities (high-frequency power, low-frequency phase, and their coupling) track auditory inputs, and they are associated with distinct perceptual mechanisms (enhancing sensitivity or decreasing reaction time), and these mechanisms are coordinated to proactively track rhythmic regularity and enhance audition. The findings start the discussion of answering how the human brain is able to process and understand the information in rapid speech and musical streams.

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Bedload transport is a complicated phenomenon in gravel-bed streams. Several factors account for this complication, including the different hydrologic regime under which different stream types operate and the wide range of particle sizes of channel bed material. Based on the hydrologic regime, there are two common types of gravel-bed streams: perennial and ephemeral. In terms of channel bed material, a gravel bed may have either unimodal or bimodal sediment. This study examines more closely some aspects of bedload transport in gravel-bed streams and proposes explanations based on fluvial mechanics. First, a comparison between perennial and ephemeral gravel-bed streams is conducted. This comparison demonstrates that under a wide range of Shields stresses, the trends exhibited by the bedload transport data of the two stream types collapse into one continuous curve, thus a unified approach is warranted. Second, an empirical bedload transport relation that accounts for the variation in the make-up of the surface material within a wide range of Shields stresses is developed. The accuracy of the relation is tested using available bedload transport data from streams with unimodal sediment. The relation is also compared against other formulae available in the literature that are commonly used for predicting bedload transport in gravel-bed streams. Third, an approach is proposed for transforming the bimodal sediment into two independent unimodal fractions, one for sand and another for gravel. This transformation makes it possible to carry out two separate computations of bedload transport rate using the bedload relation developed in this study for unimodal sediment. The total bedload transport rate is estimated by adding together the two contributions. / Ph. D.

In the past 10 years there has been a significant amount of research into two-phase particle transport. The terrorist events of September 11, 2001 sparked a series of studies analyzing particle entrainment and deposition in turbulent airflows. One area of research needing further attention has been the study of particles entrained in axisymmetric air jets. An experimental rig was designed and built to study entrainment properties and deposition of Newtonian particles, after injection into a turbulent axisymmetric free air jet. Newtonian spherical particles, ranging from 1mm to 6mm in diameter, were injected into a turbulent airstream and blown through a nozzle into a large, open space. As the particles fell out of the jet stream, their linear distances, from nozzle to initial-ground-contact, were recorded and analyzed. The experiments conducted indicated particle size and density to be significant factors when considering Newtonian particle entrainment. Additionally, particle deposition distribution revealed a consistent positive skewness, as opposed to an expected Gaussian form. The data presented in this paper provide a starting point for understanding entrainment of Newtonian spherical particles in jets. The simple experimental rig geometry and results also provide an opportunity for computational fluid dynamics models to be validated, answering a call from the 2006 Annual Review of Fluid Mechanics. / Master of Science