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The use of constructed wetlands and wet detention basins has proven to be highly effective in removing pollutants from industrial discharges and stormwater runoff throughout the world. This is attributed to design of the key treatment components in a constructed wetland, catchment source characteristics and climatic conditions. A disproportionate amount of research and monitoring effort has gone into constructed wetlands due to their cost effectiveness and ability to optimize multiple benefits. In Western Australia, several wetland monitoring studies on the role of constructed wetlands especially in Swan-Canning estuary have been done, but often do not address their design efficiencies in stormwater treatment. Two wetlands (Liege St and Tom Bateman wetland) constructed for nutrient stripping proximal to the Swan-Canning estuary have been monitored for two years. Liege St wetland was constructed to reduce the nutrients reaching the Canning River directly and improve the amenity value of the area. Similarly, Tom Bateman wetland was constructed to reduce nutrients of the Banister Creek catchment draining into the Canning River as well as for stormwater management and habitat use. Physicochemical and biological indicators were used to assess the nutrient stripping efficiency of the wetlands. In some cases, data from previous studies were used to determine the health and viability of the selected wetland sites. The limnological indicators used included; dissolved oxygen, pH, water temperature, electrical conductivity and nutrient levels. The biological included; bacteria, nutrients and chlorophyll in periphyton, macroinvertebrates and diatoms. Differences in the community structure of periphyton, macroinvertebrates and water quality were found from the inlet to the outlet in both Liege St and Tom Bateman wetlands. / Despite the poor water quality, Liege St wetland exhibited significant nutrient removal efficiencies for TP while Tom Bateman wetland had very high removal efficiency for TN. The TP removal in Liege St wetland was attributed to the design of key treatment components which included a gross pollutant trap, concrete lined sedimentation pond, vegetated sumplands, weirs and clay lining for the wetland bed. In contrast, Tom Bateman wetland lacked the above key treatment components. Additionally, the wetland experienced short-circuiting especially during high flow periods. The high TN removal in Tom Bateman wetland was attributed to assimilation by plants and micro-organisms especially by the dense growth of Potamogeton crispus observed on the wetland floor and the non- biological transformation processes such as volatilisation, sorption and sedimentation. The poor water quality of the inflow in both wetlands was attributed to catchment characteristics which were not fully investigated in this study. In an attempt to improve the nutrient stripping function of Liege St and Tom Bateman wetland, changes to the wetland design and routine maintenance were suggested for Tom Bateman and Liege St wetland respectively. Also the use of the Swan-Canning Cleanup Programe (SCCP) water quality targets as opposed to the ANZECC trigger values in water quality assessments in constructed wetlands in the Swan-Canning estuary is suggested among others.

The knowledge and understanding of sediment transport is essential for the development of effective management strategies for nutrient and sediment loading in estuarine systems. Estuarine suspended sediment (in high concentrations), has the ability to adversely impact upon surrounding ecosystems, such as the Great Barrier Reef, Australia. Due to a recent decline in water and sediment quality, it has recently been mandated that a number of tropical, coastal estuarine systems in Queensland, such as the Fitzroy River estuary, have their sediment loading reduced. In order to meet these requirements, a greater understanding of the sediment transport dynamics and driving processes (such as flocculation and settling velocity) needs to be achieved, and the accuracy of estimation improved. This research project was motivated by the need to improve the general accuracy of field measurements for estuarine suspended sediment transport and dynamics. Field-based measurements (especially settling velocity) are necessary for the parameterisation of sediment transport models. The difficulty in obtaining accurate, in situ data is well documented and is generally limited to methods that isolate a water sample from its natural environment, removing all influences of estuarine turbulence. Furthermore, the water samples are often extracted from points (Eulerian) where the history of the suspended particles is generally unknown. These sampling methods typically contain intrinsic errors as suspended sediment transport is essentially Lagrangian (i.e., flows with the net motion of flow-field) in nature. An investigation into different drogue systems conducted in parallel with a study into the tidal states of the Fitzroy River estuary led to the development of a novel Lagrangian drogue device, the LAD. Additionally, the water-tracking ability of the LAD was tested and found to accurately follow a parcel of estuarine water over a slack water period. Therefore the LAD was deployed in the Fitzroy River to assist in the further understanding of complex sediment transport processes such as flocculation and settling velocity in a natural estuarine flow field. The final device (the LAD - Lagrangian Acoustic Drogue) was developed, utilizing the principals of acoustic backscatter intensity-derived SSC measurements. The investigation of a series LAD deployments (during slack water) in the Fitzroy River estuary, revealed the dominant suspended sediment processes and also gave an insight into the prevailing flow-patterns. Results showed the presence of a settling lag mechanism between low and high tide, which can initiate a net sediment flow upstream with each flood tide. The bulk settling velocity showed comparable results at both low and high water. The LAD derived bulk settling velocity as a function of concentration (SSC), yielding a strong positive correlation (r2 = 0.73). Also the importance of flocculation in the bulk settling and clear up of the water column during periods of still water (high and low tide) was demonstrated as all in situ settling velocities (0.33 - 1.75 mm s-1) exceeded single grain approximations (0.47 mm s-1). This research demonstrates the potential for Lagrangian drogue studies as an effective measuring platform for the accurate quantification of estuarine suspended sediment dynamics. The application of the LAD in the Fitzroy River has lead to a significant improvement in the understanding of the system's real sediment transport processes. This research has provided an effective and accurate technique for measuring real settling velocities for input into numerical models or for the validation of existing model outputs. Furthermore, this technique shows great potential for application in other estuarine systems.

Hydraulic lock structures have been used for hundreds of years to control and maintain water levels in waterways. The most common are gated water regulation structures used to catch and divert water, and form an essential and critical part of many flood control and agricultural schemes. Although there are clear economic advantages to building the structures, they can contribute to major water quality problems for the waterways they influence (i.e. increased residence times and a change in mixing ability). Further, in most cases, the methods previously used to assess how the structures and their operations influence the flow regimes between the two connected systems were limited, thus hydraulic designers rely on simple formulations, existing literature and experience. Consequently, the objectives of this thesis were to undertake a detailed field study and develop a methodology and computer simulation tool to calculate the flow through a hydraulic structure connecting two water bodies so that future designs can be undertaken based upon sound knowledge. To demonstrate the outcomes of this thesis, the methodology and model were applied to an existing hydraulic structure (referred to as Structure C). Structure C is used to connect and exchange water between the tidally dominated section of the Nerang River estuary and an artificial lake system (Burleigh Lakes) on the Gold Coast, Australia. The gates of this structure open four times each day (once during each semi-diurnal tidal phase) and remain open for a period of 2 hours, allowing alternative and partial exchange between the two water bodies. To gain a better understanding of the dynamics of each waterbody under the influence of the structure, a series of detailed field experiments were initially undertaken to understand and quantify the exchange of water and its mixing ability. Tide gauges deployed within the lake indicated a water level change during each opening of up to 22 cm, equating to 413,600 m3 of water entering the lake over the 2 hour discharge period. Salinity profiles showed that the structure permitted the exchange of saline and freshwater between the two systems, during each tidal cycle, in turn maintaining the lake system as a saline (brackish environment). However, the field study also revealed that the controlled exchange of water between the systems perpetuated a permanently stratified environment on both sides of the structure. To simulate the flow dynamics influenced by Structure C, new routines were incorporated into an existing hydrodynamic model (BFHYDRO) within the model's grid and computational code, as part of this thesis. To achieve this, the flow in and out of the hydraulic structure cell (used to represent the hydraulic structure's location within the model grid) was calculated entirely from the local water level gradients on either side of the structure at each time-step, and not prescribed. This was found to be essential for complex tidally-dominated systems, such as the Nerang River. Routines were also developed to replicate the opening and closing times of the gates. Following the development of the methodology, the hydraulic structure cells were tested and applied to simulate the flow through Structure C and the complex exchange between the estuary and lake, in 2 and 3-dimensions. Tests indicated that the opening and closing times of the gates and the calibration of the discharge coefficient (which forms part of the broad-crested weir formula) were the most sensitive parameters to ensure the correct volume of water exchange between the two systems. Statistically, the model-predicted results compared very well with available surface elevation data within the estuary and lake, and thus, quantified the ability of the hydraulic structure cells to simulate the flux between the estuary and lake for each opening. Following the model validation process, results from the existing configuration were compared with hypothetical design alternatives and are documented herein. Further, part of the thesis also explored a practical and effective computer based learning strategy to introduce and teach hydrodynamic and water quality modelling, to the next generation of undergraduate engineering students. To enhance technology transfer a computer based instructional (CBI) aid was specifically developed to assist with the setup, execution and the analysis of models' output, in small easy steps. The CBI aid comprised of a HTML module with links to recorded Lotus Screen cam movie clips. The strategy proved to be a useful and effective approach in assisting the students to complete the project with minimum supervision, and acquire a basic understanding of water quality modelling. Finally, it is anticipated that this new modelling capability and the findings detailed herein will provide managers with a valuable tool to assess the influence of these structures on water circulation for present and future operations within the region. This model can also be set up at other sites to pre-assess various design configurations by predicting changes in current flows, mixing and flushing dynamics that a particular design might achieve, and assist with the selection process before the final selection and construction.

The following four broad aims were addressed in this study. (1) To ascertain whether the characteristics of the benthic macroinvertebrate assemblages within the different nearshore marine habitat types identified by Valesini et al. (2003) on the lower west coast of Australia differ significantly, and whether the pattern of those spatial differences matches those among the environmental characteristics that were used to distinguish those habitat types; (2) To develop a quantitative approach for classifying nearshore habitats in estuarine waters that employs readily-available data for a range of enduring environmental characteristics, and to use that approach to classify the various habitat types present in nearshore waters of the Swan-Canning Estuary on the lower west coast of Australia; (3) To test the hypothesis that the characteristics of the benthic macroinvertebrate assemblages in the in the Swan-Canning Estuary differ significantly among nearshore habitat types, and that the pattern of those differences matches that among the environmental characteristics used to distinguish those habitat types and (4) To test the hypothesis that, as a result of environmental changes in the Swan-Canning Estuary, the characteristics of the benthic macroinvertebrate assemblages at various habitats in this estuary in 1986/7 differ from those in 2003/4. To address the first aim, benthic macroinvertebrates were sampled seasonally for one year in the subtidal waters and intertidal zone (upper and lower swash zones) at the six nearshore habitat types that were identified by Valesini et al. (2003) on the lower west coast of Australia. The habitat types, which differed mainly in the extent of their exposure to wave activity and whether seagrass and/or nearshore reefs were present, had been distinguished quantitatively using values for a suite of seven statistically-selected enduring environmental characteristics. The faunal samples yielded a total of 121 species representing eight phyla, among which the Polychaeta, Malacostraca and Bivalvia were the most speciose classes and contributed ~ 38, 23 and 10%, respectively, to the total number of individuals. The total number of species and mean density of macroinvertebrates was far greater at the most protected habitat type (1), which also contained dense beds of seagrass, than at any other habitat type, i.e. 70 species and 209.2 individuals 0.1 m-2, compared to 32 species and 36.9 individuals 0.1 m-2 at the most exposed habitat type (6), which had a substrate comprised only of sand. Differences among habitat type influenced the benthic macroinvertebrate species composition to a greater extent than differences among either zones or seasons. Significantly different faunal compositions were detected among those latter two factors only at the most protected habitat type. The faunal assemblage at habitat type 1 was clearly the most distinct from those at the other five habitat types, particularly in the subtidal zone (R-statistics=0.642-0.831, p=0.1%), and was typified by five abundant polychaete species that were adapted to deposit-feeding. In contrast, the fauna at habitat type 6 was typified by four crustacean species and a species of bivalve and polychaete, whose mobility and tough external surface facilitated their survival and feeding in those turbulent waters. The extents of the differences in species composition among the six habitat types was significantly matched with that among the suite of enduring environmental characteristics that distinguished those habitat types, particularly in the case of the subtidal zone (Rho=0.676). Such results indicated that the environmental variables used to distinguish the nearshore habitat types could be used to reliably predict the types of benthic macroinvertebrate species likely to occur at any site along the lower west coast of Australia. The above biological validation of the nearshore marine habitat classification scheme developed by Valesini et al. (2003) provided the justification for the approach to the second broad aim of this study, namely to develop a quantitative scheme for classifying habitat types in the Swan-Canning Estuary. This approach was similar to that employed by Valesini et al. (2003) in that it considers that differences among habitat types are well reflected by differences in a suite of enduring environmental variables. However, it improves on that earlier method by employing a completely objective and quantitative approach. Thus, a large number of environmentally-diverse nearshore sites (102) were initially selected throughout the Swan-Canning Estuary and a suite of 13 enduring environmental variables quantified at each using remotely-sensed images of the estuary in a Geographic Information System. Such variables were chosen to reflect either (i) the type of substrate and submerged vegetation present, (ii) the extent of exposure to wave action or (iii) the location of the site within the estuary with respect to its vicinity to marine and fresh water sources. These data were then subjected to the CLUSTER routine and associated SIMPROF procedure in the PRIMER v6 multivariate statistical package to quantitatively identify those groups of sites that did not differ significantly in their environmental characteristics, and thus represented habitat types. Eighteen habitat types were identified, which were shown to well reflect spatial differences in a suite of non-enduring water quality and sediment characteristics that were measured in situ at a range of estuarine sites during both summer and winter in 2005 (Rho=0.683 and 0.740, respectively, p=0.1%). However, those latter environmental characteristics required far more time in the field and laboratory to quantify than the enduring variables used to identify the habitat types. Benthic macroinvertebrates were sampled during summer and winter in 2005 in the shallow subtidal regions (~1 m depth) at sites representing eight of the habitat types identified in the Swan-Canning Estuary. These samples contained a total of 51 and 36 species during summer and winter, respectively, and, in both seasons, represented nine phyla, namely Annelida, Crustacea, Mollusca, Sipuncula, Nematoda, Platyhelminthes, Cnidaria, Uniramia and Nemertea. The compositions of the benthic macroinvertebrate assemblages differed significantly among habitat types and, to a similar extent, between seasons (Global R-statistic=0.408 and 0.409, respectively, p=0.1%). However, the spatial differences were considerable greater in winter than in summer (Global R-statistic=0.536 vs 0.280, p=0.1%), presumably due to the greater spatial variation in particular non-enduring in situ environmental characteristics, such as redox depth and salinity. While the number of species, overall density and taxonomic distinctness of benthic macroinvertebrates also differed significantly among habitats, those variables differed to a greater extent between seasons, being greater in winter than in summer. While the measures of taxonomic distinctness tended to be greater at habitat types located in the lower to middle reaches, i.e. habitat types 6, 7, 9, 10, 13 and 18, than the upper reaches i.e. habitat types 1 and 3, the number of species and overall density reflected this trend only during winter. During summer, the mean numbers of species at habitat types 1, 3, 6 and 10 (3.4-6.0) were significantly lower than those at habitat types 7, 13, and 18 (8.8-10.9), whereas the overall density of benthic macroinvertebrates was far greater at habitat type 7 (32260 individuals 0.1 m-2)than at any other habitat type in this season (3135-18552 individuals 0.1 m-2). Overall, the greatest differences in assemblage composition occurred between those at habitat types 1 and 18 (R-statistic=0.669, p=0.1%), which were located in the uppermost region of the estuary and the lower reaches of the basin, respectively, and differed to the greatest extent in their enduring environmental characteristics. The assemblage at habitat type 1, and also that at habitat type 3, located just downstream, were relatively distinct from those at all other habitat types, particularly during winter (R-statistics=0.666-0.993, p=0.1%). The fauna at the first of these habitat types was relatively depauperate, containing low numbers of species and densities, and was characterised by the polychaetes Leitoscoloplos normalis and Ceratonereis aequisetis and the bivalve Arthritica semen. The assemblage at habitat type 3 was also characterised by those three species and the amphipod Paracorophium minor and the polychaete Boccardiella limnicola. In contrast, the assemblage at habitat type 18 was characterised by a more diverse assemblage, i.e. the polychaetes Capitella capitata, C. aequisetis, L. normalis and Pseudopolydora kempi, the amphipods, Grandidierella propodentata and Corophium minor and the bivalve Sanguinolaria biradiata. The number of species was among the highest at this habitat type during both seasons, which was also reflected in the high taxonomic diversity, and the overall density was the highest in winter and second highest in summer. Despite the above faunal differences, those between assemblages at habitat types 7 and 9, which were both located in the basin of the Swan-Canning Estuary, were similar in magnitude to those that occurred between pairs of habitat types located in two different regions of the estuary. Although both habitat types 7 and 9 were characterised by a similar suite of species, i.e. Oligochaete spp., C. aequisetis, C. capitata, C. minor, G. propodentata, L. normalis, and S. biradiata, the substantial differences in assemblage composition between these habitat types in both summer and winter (R-statistics=0.570 and 0.725, respectively) was due to marked differences in the relative contributions of each of these species. Significant and strong correlations were shown to exist in both summer and winter between the pattern of differences in the benthic macroinvertebrate assemblages among habitat types and that among the enduring environmental characteristics used to identify those habitat types (Rho=0.625 and 0.825, respectively, p=0.1%). Furthermore, these correlations were greater than those obtained between the benthic macroinvertebrate fauna and any combination of the non-enduring environmental characteristics (i.e. water quality and sediment parameters) recorded in situ at each habitat type (Rho=0.508 and 0.824, in summer and winter, respectively, p=o.1%). This demonstrates the greater capacity of surrogate enduring environmental characteristics to account for differences in the range of variables that may influence the distribution of benthic invertebrate fauna. Thus, the lists of characteristic benthic macroinvertebrate taxa produced for each of the eight habitat types studied in the Swan-Canning Estuary provide a reliable benchmark by which to gauge any future changes in those fauna. Moreover, these results indicate that the above habitat classification scheme can be used to reliably predict the types of benthic macroinvertebrate fauna that are likely to occur at any nearshore site of interest in this estuarine system. The final component of this study showed that the benthic macroinvertebrate assemblages at four sites in the middle reaches of the Swan-Canning Estuary in 2003/4 differed significantly from those recorded at the same sites in 1986/7. Such differences were reflected in (1) changes in the relative densities of a suite of ten species that were responsible for distinguishing the faunas in these two periods, (2) the absence of 22 rare species in 2003/4 (i.e. 42% of the number of species recorded in 1986/7), (3) the presence of 17 new species in 2003/4, including an abundant polychaete that is likely to have been introduced and (4) a far greater extent of seasonal variation in the number of species and densities of benthic macroinvertebrates in 2003/4. Such changes are likely to be related to lower sediment oxygen levels in certain seasons in 2003/4, as well as an altered hydrological regime due to increased temperatures and decreased rainfall in that more recent period. The fact that these changes have occurred within the Swan-Canning Estuary highlights the need for effective management tools, such as the habitat classification scheme and associated faunal survey undertaken in this study. Such data will provide a sound basis by which to examine the ways in which fauna vary spatially within the system, and allow for the establishment of comprehensive benchmarks for detecting future changes.

Includes bibliographical references (p.166-167)

<p>Coastal eutrophication is a complex process that is caused largely by anthropogenic nutrient enrichment. Estuaries are particularly susceptible to nutrient impairment, owing to their intimate connection with the contributing watersheds. Estuaries experiencing accelerating eutrophication are subject to a loss of key ecological functions and services. This doctoral dissertation presents the development and implementation of an integrated approach toward assessing the water quality in the Neuse Estuary following the implementation of the total maximum daily load (TMDL) program in the Neuse River basin. In order to accomplish this task, I have developed a series of water quality models and modeling strategies that can be effectively used in assessing nutrient based eutrophication. Two watershed-level nutrient loading models that operate on a different temporal scale are developed and used to quantify nitrogen loading to the Neuse Estuary over time. The models are used to probabilistically assess the success of the adopted mitigation measures in achieving the 30 % load reduction goal stipulated by the TMDL. Additionally, a novel structure learning approach is adopted to develop a Bayesian Network (BN) model that describes chlorophyll dynamics in the Upper Neuse Estuary. The developed BN model is compared to pre-TMDL models to assess any changes in the role that nutrient loading and physical forcings play in modulating chlorophyll levels in that section of the estuary. Finally, a set of empirical models are developed to assess the water quality monitoring program in the estuary, while also exploring the possibility of incorporating remotely sensed satellite data in an effort to augment the existing in-situ monitoring programs.</p> / Dissertation

Fixed nitrogen is important as a nutrient for organic matter formation and as an electron donor (nitrification) and acceptor (denitrification) for energy generation, but it is scarcely available in aquatic systems. Nitrification oxidizes ammonium to nitrite and nitrate. Denitrification uses these fixed species to form dinitrogen gas. The classic understanding of the nitrogen cycle requires dissolved oxygen for nitrification and assumes denitrification reduces nitrate to dinitrogen through various intermediates in anaerobic conditions. The global nitrogen budget is imbalanced with more marine denitrification measrued than previously estimated in the classic nitrogen cycle, suggesting alternative anaerobic nitrification and denitrification pathways exist. One alternative denitrification pathway is anammox, which directly oxidizes ammonium to dinitrogen with nitrite as the electron acceptor. Other alternative pathways for both nitrification and denitrification involve redox metals as catalysts. Manganese-catalyzed anaerobic nitrification and denitrification are thermodynamically favorable at neutral pH. However, experimental evidence for these processes is still lacking. This investigation seeks to uncover evidence of manganese-catalyzed nitrification and denitrification in saltmarsh sediments. Batch reactors with anaerobic sediment slurries from a saltmarsh in coastal Georgia were incubated in the presence and absence of colloidal manganese oxides and isotope-labeled ammonium and nitrate to trace dinitrogen formation. Results show that denitrification is more prominent in the manganese-treated reactors and that the classic denitrification pathway may not be substantial in shallow saltmarsh sediments. These data indicate that anammox and/or manganese-coupled denitrification are major contributors to the removal of fixed nitrogen. Ammonium removal in the manganese-treated reactors is accompanied by a high nitrite production compared to the nitrogen-only treatment, indicating manganese-coupled denitrification exists and/or anammox is promoted in the presence of manganese. Primary productivity is generally high in saltmarshes, but oxygen penetrates less than a few millimeters in the sediment. These observations suggest that oxygenic nitrification does not fuel denitrification below the sediment-water interface. The data show that manganese may play a role in the formation of nitrite and nitrate in oxygen-limited sediments.

Water, suspended particle and sediment samples from Kao-ping estuary were collected and measured for concentrations of polycyclic aromatic hydrocarbons (PAHs) during March 2004 and April 2005. In addition, sediments from neighboring coastal area were also analyzed to estimate distribution, transportation and possible sources of PAHs. Total PAH concentrations varied from 33.0 to 910 ng/g dry weight (dw) in coastal sediments, and diagnostic ratios reflect a mixed sources of petrogenic and pyrolytic inputs. Due to the contribution of Kao-ping River, spatial distribution of PAH concentrations at coastal sediments near river mouth varied dramatically. Results of hierachical cluster analysis showed that PAH concentration distribution was influenced by Kao-ping canyon, and biogenic source might be the major PAH source for offshore sediments. Total PAH concentrations in river sediment varied from 63.0 to 720 ng/g dw. Higher concentration was measured between the Water Main pipe and Shuang-yuan Bridge, and possible sources were from both petrogenic and pyrolytic sources. Sediment of Dung-gang harbour had highest concentration, 28,000ng/g dw, in this study, which was contributed from petrogenic sources due to its intensive boating activities. Except fluorene and phenanthrene in harbour sediments, individual PAH concentrations of other sediments are lower or near the Effect Range Low value, concentrations might lead to possible adverse effects upon organism. Total PAH concentrations varied from 5.0 to 82.0 ng/L in suspended particulate phase and from 5.5ng/L to 46.0ng/L in dissolved phase, respectively. Most of high molecular weight PAH concentrations (>5-ring PAHs) in dissolved phase were below method detection limits. The partition coefficients¡]Koc¡^values of PAHs were 1 to 2 orders higher than predicted values. It might be attributed to soot particles which have extremely high sorption capacities. Correlation coefficients between total PAH concentrations in sediments versus total organic carbon¡]TOC¡^ and fine particle content¡]<63£gm%¡^were significant ¡]R=0.575, 0.800, 0.851 and 0.657, P<0.01¡^. In addition, PAHs in suspended particulate phase and dissolved phase were also significantly correlated to particulate organic carbon¡]POC¡^ and dissolved organic carbon¡]DOC¡^, respectively. The distribution of calculated PAH concentrations from organic carbon was higher in surface water than bottom water. Unlike salinity, there was no decreasing or increasing trend of these concentrations among river samples. It is possible that contamination was not come from upstream, but from estuary area where plume was lifted and diffused upstream by neat seawater. The flux in Wan-da Bridge was higher than downstream estuary area that might be due to PAH concentrations reduction by sedimentation or degradation.

Understanding the salt marsh ecosystem in the Guadalupe Estuary is needed because wetlands in this system support the endangered whooping crane (Grus americana). The marsh plant research and monitoring described herein were based in the salt marshes at Aransas National Wildlife Refuge (ANWR), which are utilized by the cranes each winter. Past research indicates that the Carolina wolfberry (Lycium carolinianum) contributes 21-52% of crane energy intake early in the wintering period (Chavez 1996). Beginning in Fall 2003, vegetation transects were sampled along an estuarine gradient at ANWR. Species diversity and composition was similar at the three sites, with all sites containing the same 6-7 common species. While Spartina alterniflora is only a minor part of this vegetation community, it dominates the few low inter-tidal, fringe areas present. Species composition exhibited little variability from Year 1 to Year 2 of the study. Densities and biomass of L. carolinianum were not significantly different between sites or years. L. carolinianum, while important to salt marsh ecology, accounts for only a small portion of the overall productivity. Based on correlation coefficients, L. carolinianum was found in association with some of the common species in the vegetation community, indicating that its growth and survival requirements are typical to the salt marshes at ANWR. Also beginning in Fall 2003, I repeatedly sampled L. carolinianum in permanent plots along the estuarine gradient. L. carolinianum exhibits strong temporal patterns. Leaf production peaked in early spring and again just prior to peak berry abundance. Flowering of L. carolinianum occurred in October and November. Peak berry abundance coincided with the cranes’ arrival in late October and early November. Berry production occurred in October, November, and December; berries were virtually non-existent in the marshes for the remainder of the year. Stepwise regression showed stem diameter alone was a good estimator of aboveground biomass of this species in ANWR marshes, accounting for 94% of the variability (p<0.001). Changes in aboveground biomass followed no distinct patterns in the year of monitoring, perhaps due to the woody stem of the plant. Spatial patterns in L. carolinianum were not explained by water quality parameters alone; it is suggested that soil properties may help to account for the spatial variability.