Spelling suggestions: "subject:"anevolution anda devevelopment (0412)"" "subject:"anevolution anda agentdevelopment (0412)""
1 |
Contribution of the canonical Wnt pathway in Tribolium anterior-posterior axis patterningFu, Jinping January 1900 (has links)
Doctor of Philosophy / Department of Biology / Susan J. Brown / How animals polarize and establish the main axis during embryogenesis has been one
of the most attractive questions in Biology. Increasing body of work in various model
organisms implicates that most metazoans utilize the canonical Wnt signaling pathway to
pattern the anterior-posterior (AP) axis, despite the limited evidence from arthropods. In
Drosophila, a highly derived insect, canonical Wnt activity is not required for global AP
patterning, but in typical insects including Tribolium castaneum, loss of canonical Wnt
activity results in posterior truncation. To determine the eff ects of increased canonical Wnt
levels, I analyzed the function of axin, encoding a highly conserved negative regulator of the pathway. Tc-axin transcripts are maternally localized to the anterior pole in freshly laid
eggs. Parental RNAi for Tc-axin produced progeny phenotypes that ranged from mildly
a ffected embryos with cuticles displaying a graded loss of anterior structures, to severely
a ffected embryos lacking cuticles and condensing to the posterior pole of the egg without any de finable structures. Altered expression patterns of several blastodermal markers indicated anterior expansion of posterior fates. Epistasis analysis of other canonical Wnt pathway components and the expansion of Tc-caudal expression, a Wnt target, suggest that the eff ects of Tc-axin depletion are mediated through this pathway and that canonical Wnt activity must be repressed for proper anterior development in Tribolium. These studies provide unique evidence that canonical Wnt activity must be carefully regulated along the AP axis in an arthropod, and support an ancestral role for Wnt signaling in de fining AP polarity and patterning in metazoan development.
Additionally, as an anterior structure, the extraembryonic serosa is reduced in Tc-axin
RNAi progeny. However, in Tc-pangolin (Tc-pan, a homolog of Wnt downstream component) RNAi progeny, an interesting phenotype was produced that serosa was not only reduced but also separated into distinct anterior and dorsal domains. I carefully recorded this phenomenon with live imaging using a Tribolium transgenic line that expresses GFP in each nucleus. Through careful examination with embryonic fate-map markers, I found that the tissue between separated serosa domains is dorsally extended head lobe. And I also found that in severe phenotype, dorsal serosa was completely gone while anterior serosa not, suggesting independent regulation mechanisms for anterior and dorsal serosa formation. This descriptive data will complement future study in the genetic mechanism underlying serosa formation by providing more details in morphogenesis.
|
2 |
Genomics and physiological evolution of cold tolerance in Drosophila melanogasterGerken, Alison Renae January 1900 (has links)
Doctor of Philosophy / Division of Biology / Theodore J. Morgan / Thermal stress impacts animals around the globe and understanding how organisms adapt to changes in temperature is of particular interest under current climate change predictions. My research focuses on the evolutionary genetics involved in cold tolerance and plasticity of cold tolerance using both artificially selected and naturally segregating populations, while tying the genes of interest to their physiological components. First I address cross-tolerance of stress traits following artificial selection to a non-lethal cold tolerance metric, chill-coma recovery. Using these artificial selection populations, we found that stress traits such as desiccation tolerance, starvation tolerance, acclimation, and chronic and acute cold tolerance do not correlate with level of cold tolerance as defined by chill-coma recovery time. We next assessed lifetime fitness of these different cold tolerance lines and found that only at low temperatures did fitness differ among cold tolerance levels. We then analyzed gene expression differences between resistant and susceptible populations at three time points to understand where selection pressures are hypothesized to act on genomic variation. Our gene expression analyses found many differences between resistant and susceptible lines, primarily manifesting themselves in the recovery period following cold exposure. We next utilized a community resource, the Drosophila melanogaster reference panel, to identify naturally segregating variation in genes associated with cold acclimation and fitness. We specifically asked if long- and short-term acclimation ability had overlapping genetic regions and if plasticity values from constant rearing environments were associated with demographic parameters in fluctuating environments. We found that long- and short-term acclimation are under unique genetic control and functionally tested several genes for acclimation ability. We also found that acclimation ability in constant environments and fitness in fluctuating environments do not correlate, but that genotypes are constrained in their fitness abilities between a warm and cool environment. Our analyses describe several novel genes associated with cold tolerance selection and long- and short-term acclimation expanding our knowledge of the complex relationship between demographic components and survivorship as well as a unique investigation of the change in gene expression during cold exposure.
|
3 |
Family planning in context: sensitivity of fertility desires and intentions to ecological cuesAdair, Lora E. January 1900 (has links)
Doctor of Philosophy / Department of Psychological Sciences / Gary Brase / Although fertility decision-making has been the source of considerable theoretical and empirical investigation, the effect of several contextual variables on individuals’ fertility decision-making processes are not yet understood. For example, are individuals more strongly influenced by social forces that are informational or normative? Also, do individuals change their fertility intentions based on their current and developmental economic conditions? Further, how ‘shared’ are reproductive decisions within a couple, are males or females more likely to get what they want? This 3-study program of research used both experimental and exploratory qualitative methods to elucidate the nature of these unresolved issues within the domain of fertility decision-making. Study 1 (N = 344, M[subscript]age = 23, SD[subscript]age =6.41, 59.3% female) found that highly motivated individuals’ fertility intentions were more susceptible to informational, compared to normative messages (the opposite was true for unmotivated participants). Study 2 (N = 249, M[subscript]age = 24, SD[subscript]age =6.10, 61.4% female) found that exposure to mortality primes up-regulated fertility intentions for individuals with “fast” life history strategies, but facilitated the down-regulation of fertility intentions for individuals with “slow” life history strategies. Interestingly, resource scarcity primes were associated with the postponement of fertility plans in individuals’ with “fast” life history strategies. Study 3 (N = 120, M[subscript]age = 21, SD[subscript]age =4.96, 50% female) found that, contrary to predictions, the similarity of couples’ gender role attitudes, career-orientations, and education levels did not significantly predict the frequency of their use of statements coded as compromise and agreement or persuasion and disagreement in their discussions regarding their future reproductive plans. Findings do suggest that individuals with higher levels of education were more likely to use persuasion and disagreement statements in their child timing and number discussions with their romantic partner, indicative of greater decision-making power in that particular social exchange. Further, men and women in study 3 were equally likely to use statements coded as compromise and agreement, persuasion and disagreement, and concession when discussing both their future fertility plans as well as their future financial plans.
|
4 |
Patterns of reproductive allocation in aphidophagous lady beetles and their response to various levels of resource availabilityVargas Orozco, German Andres January 1900 (has links)
Doctor of Philosophy / Department of Entomology / J.P. Michaud / James R. Nechols / The manner in which organisms allocate reproductive resources for reproduction is a central question with respect to life history theory. The main objectives of this research were to i) examine lifetime patterns of reproductive allocation in the lady beetles Coleomegilla maculata (DeGeer) and Hippodamia convergens (Guérin-Menéville) (Coleoptera: Coccinellidae) while manipulating environmental conditions that affect female body size (i.e., larval food supply), ii) to study the interaction between factors underlying female body size and the resources available during reproduction, and iii) to explore the maternal effects of female size and age on the development and survival of progeny. When different size classes of females were produced and adult females were maintained with unlimited food, there were no differences in egg size across female size in C. maculata, but egg size increased over time in all females. In H. convergens, only larger females increased egg size over time, and they laid larger eggs, on average, than did small females. Maternal body size was positively correlated with the number of eggs laid per day in both species. When three size classes of females were subjected to a fluctuating food supply as adults, female size was again positively correlated with egg and daily fecundity. Whereas both species varied daily fecundity in response to adult food supply, egg size was unaffected and demonstrated a fixed pattern of change with female age and species-specific effects of maternal body size. To observe maternal effects in H. convergens, three female size classes were again produced and progeny were reared from three different periods of each female‟s reproductive life. Offspring from later oviposition days and larger females developed faster and achieved larger adult size than those reared from earlier oviposition days. Egg size showed inconsistent correlations with developmental parameters and adult progeny size, so other, more cryptic, maternal signals were inferred to signal phenotype development in progeny. A fixed program of producing faster-developing offspring that mature to larger sizes late in the oviposition cycle is adaptive for exploiting ephemeral aphid blooms that exhibit predictable dynamics of declining prey abundance and increasing competition. In the case of H. convergens, resource limitation during development constrained not only body size, fecundity and egg size, but also maternal ability to manipulate progeny phenotypes.
|
Page generated in 0.0849 seconds